WO2012072799A1 - New methods for the in vitro measurement of the biological activity of an ultra low molecular weight heparin sample - Google Patents
New methods for the in vitro measurement of the biological activity of an ultra low molecular weight heparin sample Download PDFInfo
- Publication number
- WO2012072799A1 WO2012072799A1 PCT/EP2011/071633 EP2011071633W WO2012072799A1 WO 2012072799 A1 WO2012072799 A1 WO 2012072799A1 EP 2011071633 W EP2011071633 W EP 2011071633W WO 2012072799 A1 WO2012072799 A1 WO 2012072799A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ulmwh
- standard
- sample
- arg
- activity
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
Definitions
- the present application concerns Ultra Low Molecular Weight Heparins (ULMWH) and the assays for measuring their biological activity.
- ULMWH Ultra Low Molecular Weight Heparins
- Low Molecular Weight Heparins are defined in the European Pharmacopoeia (European Pharmacopoeia 6.0 (01/2008:0828, 2041 -2043)) by their molecular mass (less than 8000 Daltons) and their potency (an anti-factor Xa activity not less that 70 IU per milligram and a ratio of anti-factor Xa activity to anti-factor lla activity not less than 1 ,5).
- semuloparin developed by Sanofi-Aventis (AVE5026), belongs to a new generation of hemisynthetic heparins. It is a new ultra-low molecular weight heparin (ULMWH), with a mean molecular weight of 2400 Daltons and a novel antithrombotic profile resulting from high anti-factor Xa activity (-160 U/mg) and residual anti-factor lla activity (-2 U/mg), as described by C. Viskov et al. in Journal of Thrombosis and Haemostasis, 2009, vol. 7, 1 143-1 151 . Semuloparin, in the form of its sodium salt, is in clinical development for venous thromboembolism prevention.
- Semuloparin is obtained by selective and controlled depolymerization of heparin, as described in the patent application WO 2004/033503 and in J. Thromb. Hameost. ⁇ ibid). It was shown that its unique structural features result from the highly selective depolymerization of heparin by the phosphazene base that protects the antithrombin (AO- binding site from destruction.
- LMWH Low Molecular Weight Heparins
- the anti-factor Xa and anti-factor lla activities are measured by using an International Standard as defined by the European Pharmacopoeia (European Pharmacopoeia 6.0 (01/2008:0828, 2041 -2043)).
- the patent application WO 2004/033503 teaches to measure the anti-factor Xa activity of semuloparin by the amydolytic method on a chromogenic substrate as described by Teien et al., Thrombosis Research, 10, 399-410 (1977), using as a standard the first International Standard for low molecular weight heparins.
- it teaches to measure its anti-factor lla activity by the method described by Anderson L. O. et al., Thrombosis Research, 15, 531 -541 (1979), with as a standard the first International Standard for low molecular weight heparins.
- WO 2005/010051 discloses new heparin-derived oligosaccharide mixtures characterized by a mean molecular weight comprised between 1800 and 2400 Daltons, an anti-factor Xa activity comprised between 190 lU/mg and 450 lU/mg, and virtually no anti-factor lla activity. Said oligosaccharide mixtures are obtainable by a re-depolymerization process of an ULMWH.
- the anti-factor Xa activity of these mixtures is measured relative to a reference ULMWH having an anti-factor Xa activity of 140 to 180 lU/mg (on dry basis) and an anti-factor lla activity of 2,1 lU/mg, the activity of this reference ULMWH being measured relative to the International Standard for low molecular weight heparins.
- LMWH Low Molecular Weight Heparins
- LMMH Low Molecular Mass Heparins
- the inventors have identified a standard suitable for the in vitro measurement of the biological activity of an ULMWH sample, where said standard comprises said ULMWH sample.
- the present invention thus concerns a method for the in vitro measurement of the biological activity of an ULMWH sample, wherein said method is carried out relative to a standard comprising the same ULMWH.
- ULMWH Ultra Low Molecular Weight Heparins, as defined by the European Pharmacopoeia in force, supra. In the framework of the instant invention, it includes heparin derivatives having a molecular weight of 2000 to 4000 Daltons. ULMWH include in particular semuloparin.
- Semuloparin has a mean molecular weight of 2000-3000 Daltons (about 2.4 kDa on average), a high anti-factor Xa activity (approx. 160 U/mg on dry basis), a low anti- factor lla activity (approx. 2 U/mg on dry basis) and a high anti-factor Xa / anti-factor lla ratio (>30).
- the term "semuloparin” as used herein is used to refer to semuloparin in the free form or any of its salts, including semuloparin sodium salt.
- standard refers to the standard, or calibrator used for measurement of in vitro activity to assign the potency of a sample.
- the standard is generally expressed in units (U).
- the standard comprising the same ULMWH is herein referred to as "the ULMWH standard”.
- the ULMWH standard is a preparation of semuloparin.
- Said preparation is preferably a solution of semuloparin.
- the ULMWH standard has a pre-determined biological activity; more particularly, the ULMWH standard has a pre-determined anti- factor Xa activity, generally an anti-factor Xa activity of around 160 U/mg on dry basis and an anti-factor lla of around 1 to 5 U/mg on dry basis.
- the method of the invention is a dilution assay, wherein the ULMWH sample to be measured is supposed to have the same potency as the ULMWH standard.
- the measurement of the biological activity of an ULMWH sample is carried out by determination of the relative potency(ies) of said sample. This determination requires the parallelism of the curves of the ULMWH sample to be measured and those of the ULMWH standard.
- the measurement of said biological activity includes the measurement of the anti- factor Xa activity and/or the measurement of the anti-factor I la activity of an ULMWH sample.
- These assays determine the ability of said sample to accelerate the inhibition of factor Xa (anti-factor Xa activity) and/or of factor I la (or thrombin), by antithrombin III (ATI 11), according to the following reactions: ATIII + ULMWH ⁇ [ATIII-ULMWH]
- the amount of the paranitroaniline, measured at 405 nm, is inversely proportional to that of the ULMWH sample.
- curves refers to the graphic representation obtained by plotting the optical density measured in the above reaction as function of the concentration of the sample.
- the measurement of said biological activity is preferably made by adapting the method described in the monograph on LMWHs of the European Pharmacopoeia in force, such as European Pharmacopoeia 6.0 (01/2008:0828, 2041 -2043).
- said method comprises an amidolytic assay on a chromogenic substrate, according to the method described in the monograph on LMWHs of the European Pharmacopoeia in force, as above.
- Said chromogenic substrate depends on the activity to be measured.
- Suitable chromogenic substrates for measuring the anti-factor Xa activity may be chosen from the group consisting in S-2222TM (Bz-lle-Glu(g-OR)-Gly-Arg-pNA-HCI) and S-2765TM (N-a-Z- D-Arg-Gly-Arg-pNA 2HCI also referred to as N-a-benzyloxycarbonyl-D-arginyl-L-glycyl-L- arginine-4-nitroanilide dihydrochloride) both available from Chromogenix; CBS 31 .39 (CH 3 -S0 2 - D -Leu-Gly-Arg-pNA) available from Stago; BIOPHEN® CS-1 1 (22) (Mixture (50%-50%) of Bz-lle-Glu (YOCH3)-Gly-Arg-pNa (form 1 ) and Bz-lle-Glu (yOH)Gly-Arg
- Suitable chromogenic substrates for measuring the anti-factor I la activity may be chosen from the group consisting in S-2238TM (H-D-Phe-Pip-Arg-pNA-2HCI also referred to as D-phenylalanyl-L-pipecolyl-L-arginine-4-nitroanilide dihydrochloride) available from Chromogenix, CBS 34.47 (H- D -CHG-But-Arg-pNA) and CBS00.68 both available from Stago, BIOPHEN® CS-01 (81 ) (Tos-Gly-Pro-Arg-pNa.
- BIOPHEN® CS- 01 (38) H-D-Phe-Pip-Arg-pNa, 2HCI) both available from HYPHEN BioMed. More preferably, said chromogenic substrate for measuring the anti-factor lla activity is S- 2238TM.
- the method of the invention may be conducted in solutions of biological samples such as plasma, or in buffer solutions, preferably in buffer solutions.
- Buffers may be chosen from any known buffers, in particular those having a pH close to biological media, such as those buffers having a pH comprised between 6.5 and 9.
- Buffer solutions include in particular tris(hydroxymethyl)aminomethane sodium chloride pH 7.4, tris(hydroxymethyl)aminomethane-EDTA pH 8.4, and tris(hydroxymethyl)aminomethane-NaCI-PEG pH 7.4. They may be prepared according to routine, well-known procedures.
- the method of the invention comprises the following steps:
- Step a determining the biological activity of the ULMWH sample against the calibration curve obtained in step b).
- the ULMWH standard in a form such as a water for injection solution, may generally be prepared by diluting a given amount of the ULMWH into a given amount of the chosen solution such as buffer solution.
- the given amounts are advantageously expressed in volumes.
- Said ULMWH standard and ULMWH sample are generally processed in the same way, in the same medium, e.g. buffer solution.
- the ULMWH sample may be prepared in solution of water for injection, by weighing an appropriate quantity in order to obtain activities in U/mL equivalent to those of the ULMWH standard. The sample is then diluted in the same medium (e.g. buffer solution) as the ULMWH standard.
- medium e.g. buffer solution
- a potency (e.g. anti-factor Xa or anti-factor I la activity) expressed in U/mL is assigned to the given amount of the ULMWH, before dilution. A corresponding potency may thus be calculated for the diluted sample, depending on the dilution rate.
- From 4 to 14 calibration points may be performed.
- the method of the invention involves 4 or 14 calibration points depending on whether a routine procedure or experimentation procedure is carried out, respectively.
- the procedure may be carried out according to the 5-parameters methodology (in particular for the 14 calibration point method) or the parallel lines methodology (in particular for the 4 calibration point method).
- the assay for carrying out the method of the invention may be performed by known procedures, such as on a micro-plate reader and dispenser.
- the calibration curve may thus be obtained by correlating the calculated potencies with the obtained Optical Density (OD).
- the activity in U/mL of the ULMWH sample may be obtained by correlating the activity and the obtained Optical Density (OD), according to the calibration curve obtained with the ULMWH standard.
- OD Optical Density
- the anti-factor Xa activity assigned to the ULMWH standard before dilution is 100 U/mL.
- the points of the calibration curve are comprised between approximately 0.002 and 0.8 U/mL for the anti-factor Xa activity curve in the 14 calibration points method.
- the range of the calibration points for the 4 point calibration curve is generally comprised within the above range, such as 0.04-0.07 ⁇ 0.002 U/mL.
- the anti-factor I la activity assigned to the ULMWH standard before dilution is 1 .8 U/mL.
- the points of the calibration curve are comprised between 0.0007 and 0.3 U/mL for the anti-factor I la activity curve in the 14 calibration points method.
- the range of the calibration points for the 4 point calibration curve is generally comprised within the above range, such as 0.007-0.012 ⁇ 0.001 U/mL.
- the biological activity (relative potency) of the ULMWH sample is calculated from the calibration curve obtained with the ULMWH standard.
- the present invention also concerns a standard suitable for measuring the in vitro biological activity of an ULMWH sample, which comprises the same ULMWH than the sample to be measured.
- said standard comprises semuloparin.
- Said ULMWH standard is suitable for conducting the method of the invention.
- Said ULMWH standard is preferably obtained by diluting a sample of the ULMWH to be measured, which is calibrated by comparison with a recognized standard of LMWH, such as the second international standard of LMWH, currently available.
- the ULMWH standard is generally assigned an anti-factor Xa activity around 160 U/mg on dry basis and an anti-factor I la of around 2 U/mg on dry basis.
- Figures 1 and 2 illustrate the anti-factor Xa activity of bemiparin and semuloparin according to the 14 calibration points procedure.
- FIGS 3 and 4 illustrate the anti-factor I la activity of bemiparin and semuloparin according to the 14 calibration points procedure.
- Figures 5, 6, 7 and 8 illustrate the anti-factor Xa activity and anti-factor lla activity of semuloparin compared with a standard of Low Molecular Mass Heparin for assay Biological Reference Preparation batch 5 (BRP5) according to the 14 calibration points procedure.
- Figures 9, 10, 1 1 , 12 illustrate the anti-factor Xa activity and anti-factor lla activity of semuloparin compared with a heparin standard (K5, USP357) according to the 14 calibration points procedure.
- Figure 13 and 14 illustrate the anti-factor Xa activity and anti-factor lla activity of semuloparin and a standard comprising semuloparin (3000ET) according to the 4 calibration points procedure.
- the curve profile of biological activity of the semuloparin was compared to that of bemiparin, the LMWH reference standard of the European Pharmacopoeia BRP5, and the heparin standard (K5, USP357).
- the anti-factor lla and anti-factor Xa activities were measured by assays according to the following protocols: PREPARATION OF REAGENTS
- Equipment is given indicatively - any other equipment validated for this method can be used.
- PACKARD MULTIPROBE II STD Automated liquid handling system (dilution robot).
- MR 7000 or DIAS from LABSYSTEM Microplate reader, incubator and dispenser. PREPARATION OF SOLUTIONS TO BE ASSAYED
- Semuloparin the LMWH standard of the European Pharmacopoeia (BRP5), the heparin standard (K5 USP 357) and the semuloparin reference standard (3000ET) are diluted in the same way, and the ranges in U/mL or UI/mL are given in Tables 1 and 2.
- Anti-factor Xa activity the LMWH standard of the European Pharmacopoeia (BRP5), the heparin standard (K5 USP 357) and the semuloparin reference standard (3000ET) are diluted in the same way, and the ranges in U/mL or UI/mL are given in Tables 1 and 2.
- Anti-factor Xa activity the LMWH standard of the European Pharmacopoeia (BRP5), the heparin standard (K5 USP 357) and the semuloparin reference standard (3000ET) are diluted in the same way, and the ranges in U/mL or UI/mL are given in Tables 1 and 2.
- the assay was performed using MR7000 or DIAS (dynex) from LABSYSTEM.
- DIAS dinex
- Results are illustrated in Figures 1 and 2 for the anti-factor Xa activity and in figures 3 and 4 for the anti-factor lla activity.
- Results are illustrated in Figures 5 and 7 for the anti-factor Xa activity and in figures 6 and 8 for the anti-factor lla activity
- Results are illustrated in Figures 9 and 1 1 for the anti-factor Xa activity and in figures 10 and 12 for the anti-factor lla activity.
- This method may be used as a routine procedure. Anticoagulant activity of semuloparin was determined in vitro by chromogenic assays. The methods were adapted from those recommended by the European Pharmacopoeia for low molecular weight heparin (LMWH).
- LMWH low molecular weight heparin
- the biological activity of the sample was compared to that of a well characterised in-house semuloparin reference standard.
- the anti-factor lla and anti-factor Xa activities were measured by assays according to the following protocols:
- the assay is performed using MR7000 or DIAS from LABSYSTEM, ANTI-FACTOR Xa ACTIVITY:
- the relative potency is expressed in U (anti-FXa or anti-Flla)/mg on the dried basis.
- semuloparin was compared to a standard comprising semuloparin (3000ET).
- the anti-FXa and anti-Flla activities of semuloparin containing samples were determined according to the 4-points calibration methodology disclosed above, using a standard comprising semuloparin.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present application concerns Ultra Low Molecular Weight Heparins (ULMWH) and the assays for measuring their biological activity.
Description
New methods for the in vitro measurement of the biological activity of an Ultra Low Molecular Weight Heparin sample
The present application concerns Ultra Low Molecular Weight Heparins (ULMWH) and the assays for measuring their biological activity.
Low Molecular Weight Heparins are defined in the European Pharmacopoeia (European Pharmacopoeia 6.0 (01/2008:0828, 2041 -2043)) by their molecular mass (less than 8000 Daltons) and their potency (an anti-factor Xa activity not less that 70 IU per milligram and a ratio of anti-factor Xa activity to anti-factor lla activity not less than 1 ,5).
By contrast, semuloparin, developed by Sanofi-Aventis (AVE5026), belongs to a new generation of hemisynthetic heparins. It is a new ultra-low molecular weight heparin (ULMWH), with a mean molecular weight of 2400 Daltons and a novel antithrombotic profile resulting from high anti-factor Xa activity (-160 U/mg) and residual anti-factor lla activity (-2 U/mg), as described by C. Viskov et al. in Journal of Thrombosis and Haemostasis, 2009, vol. 7, 1 143-1 151 . Semuloparin, in the form of its sodium salt, is in clinical development for venous thromboembolism prevention.
Semuloparin is obtained by selective and controlled depolymerization of heparin, as described in the patent application WO 2004/033503 and in J. Thromb. Hameost. {ibid). It was shown that its unique structural features result from the highly selective depolymerization of heparin by the phosphazene base that protects the antithrombin (AO- binding site from destruction.
Generally, for Low Molecular Weight Heparins (LMWH), the anti-factor Xa and anti-factor lla activities are measured by using an International Standard as defined by the European Pharmacopoeia (European Pharmacopoeia 6.0 (01/2008:0828, 2041 -2043)).
In particular, the patent application WO 2004/033503 teaches to measure the anti-factor Xa activity of semuloparin by the amydolytic method on a chromogenic substrate as described by Teien et al., Thrombosis Research, 10, 399-410 (1977), using as a standard the first International Standard for low molecular weight heparins. Similarly, it teaches to measure its anti-factor lla activity by the method described by Anderson L. O. et al., Thrombosis Research, 15, 531 -541 (1979), with as a standard the first International Standard for low molecular weight heparins.
WO 2005/010051 discloses new heparin-derived oligosaccharide mixtures characterized by a mean molecular weight comprised between 1800 and 2400 Daltons, an anti-factor Xa activity comprised between 190 lU/mg and 450 lU/mg, and virtually no anti-factor lla activity. Said oligosaccharide mixtures are obtainable by a
re-depolymerization process of an ULMWH. The anti-factor Xa activity of these mixtures is measured relative to a reference ULMWH having an anti-factor Xa activity of 140 to 180 lU/mg (on dry basis) and an anti-factor lla activity of 2,1 lU/mg, the activity of this reference ULMWH being measured relative to the International Standard for low molecular weight heparins.
However, the inventors have now shown that the International Standard generally used in assays for Low Molecular Weight Heparins, herein called "LMWH" or Low Molecular Mass Heparins (LMMH) International Standard, is not suitable for Ultra Low Molecular Weight Heparins, such as semuloparin. More specifically, it was surprisingly shown that in determining potency of semuloparin against said LMWH International Standard using 5-parameters data reduction model, the whole curves for samples were not found parallel, thus failing to comply with the parallelism pre-requisite. The parallelism requirement between sample and standard is commonly used for the bioassays data reduction (R.G. Das et al., BioProcess International, 2008, 46-56) and recommended by the Pharmacopoeia: comparison of the high and low asymptotes and the slope of curves is recommended in chapter 5.3 of the European Pharmacopoeia and in part <1 1 1 > of the US Pharmacopeia. In particular, the methodology developed by Dunn et al. (Measuring Parallelism, Linearity and Relative Potency in Bioassay and Immunoassay Data, Journal of Biopharmaceutical Statistics, 2005, 15:437-463; The five- parameter logistic: A characterization and comparison with the four-parameter logistic, Analytical Biochemistry, 2005, 343:54-65, both incorporated herein by reference) involves inter alliae measuring parallelism by comparing two "dose-response" curves for mathematical similarity of shape, determination of logistic curves by using the 4 or 5- parameters model, determination of the distance of two sets of data from "perfect" parallelism.
In view of the lack of parallelism obtained for semuloparin, in particular when measuring the anti-factor lla activity, it appears that the LMWH International Standard is not suitable as a unique reference standard for measuring the biological activity of an ULMWH, as this leads to a lack of parallelism, and, hence, a failure in the statistical analysis of the potency estimation results, as well as a lack of compliance towards the methodology set forth in the European Pharmacopoeia for anti-factor lla and anti-factor Xa titrations.
There is therefore a need to provide straightforward and reproducible methods of measuring the biological activities of ULMWH.
More particularly, there is a need for a standard suitable for determining relative potencies of ULMWH samples such as semuloparin samples.
The inventors have identified a standard suitable for the in vitro measurement of the biological activity of an ULMWH sample, where said standard comprises said ULMWH sample.
According to a first object, the present invention thus concerns a method for the in vitro measurement of the biological activity of an ULMWH sample, wherein said method is carried out relative to a standard comprising the same ULMWH.
ULMWH is used herein to refer to Ultra Low Molecular Weight Heparins, as defined by the European Pharmacopoeia in force, supra. In the framework of the instant invention, it includes heparin derivatives having a molecular weight of 2000 to 4000 Daltons. ULMWH include in particular semuloparin.
Semuloparin has a mean molecular weight of 2000-3000 Daltons (about 2.4 kDa on average), a high anti-factor Xa activity (approx. 160 U/mg on dry basis), a low anti- factor lla activity (approx. 2 U/mg on dry basis) and a high anti-factor Xa / anti-factor lla ratio (>30). The term "semuloparin" as used herein is used to refer to semuloparin in the free form or any of its salts, including semuloparin sodium salt.
The term "standard" as used herein refers to the standard, or calibrator used for measurement of in vitro activity to assign the potency of a sample.
The standard is generally expressed in units (U).
The standard comprising the same ULMWH is herein referred to as "the ULMWH standard".
According to the instant invention, the ULMWH standard is a preparation of semuloparin. Said preparation is preferably a solution of semuloparin.
According to a preferred embodiment, the ULMWH standard has a pre-determined biological activity; more particularly, the ULMWH standard has a pre-determined anti- factor Xa activity, generally an anti-factor Xa activity of around 160 U/mg on dry basis and an anti-factor lla of around 1 to 5 U/mg on dry basis.
According to a preferred embodiment, the method of the invention is a dilution assay, wherein the ULMWH sample to be measured is supposed to have the same potency as the ULMWH standard. The measurement of the biological activity of an ULMWH sample is carried out by determination of the relative potency(ies) of said sample. This determination requires the parallelism of the curves of the ULMWH sample to be measured and those of the ULMWH standard.
The measurement of said biological activity includes the measurement of the anti- factor Xa activity and/or the measurement of the anti-factor I la activity of an ULMWH sample. These assays determine the ability of said sample to accelerate the inhibition of factor Xa (anti-factor Xa activity) and/or of factor I la (or thrombin), by antithrombin III (ATI 11), according to the following reactions: ATIII + ULMWH → [ATIII-ULMWH]
[ATIII-ULMWH] + FactorExcess → [ATIII-ULMWH-Factor] + FactorResidual
FactorRes iduai + chromogenic substrate → paranitroaniline
The amount of the paranitroaniline, measured at 405 nm, is inversely proportional to that of the ULMWH sample.
The term "curves" above refers to the graphic representation obtained by plotting the optical density measured in the above reaction as function of the concentration of the sample. The measurement of said biological activity is preferably made by adapting the method described in the monograph on LMWHs of the European Pharmacopoeia in force, such as European Pharmacopoeia 6.0 (01/2008:0828, 2041 -2043).
Generally, said method comprises an amidolytic assay on a chromogenic substrate, according to the method described in the monograph on LMWHs of the European Pharmacopoeia in force, as above.
Said chromogenic substrate depends on the activity to be measured. Suitable chromogenic substrates for measuring the anti-factor Xa activity may be chosen from the group consisting in S-2222™ (Bz-lle-Glu(g-OR)-Gly-Arg-pNA-HCI) and S-2765™ (N-a-Z- D-Arg-Gly-Arg-pNA 2HCI also referred to as N-a-benzyloxycarbonyl-D-arginyl-L-glycyl-L-
arginine-4-nitroanilide dihydrochloride) both available from Chromogenix; CBS 31 .39 (CH3-S02-D-Leu-Gly-Arg-pNA) available from Stago; BIOPHEN® CS-1 1 (22) (Mixture (50%-50%) of Bz-lle-Glu (YOCH3)-Gly-Arg-pNa (form 1 ) and Bz-lle-Glu (yOH)Gly-Arg-pNa (form 2)) available from Hyphen BioMed; BIOPHEN® CS-1 1 (32) (Suc-lle-Gly (γ Pip)Gly- Arg-pNa, HCI) available from HYPHEN BioMed; BIOPHEN® CS - 1 1 (65) (D-Arg-Gly-Arg- pNA, 2HCI) available from HYPHEN BioMed. More preferably, said chromogenic substrate for measuring the anti-factor Xa activity is S-2765™.
Suitable chromogenic substrates for measuring the anti-factor I la activity may be chosen from the group consisting in S-2238™ (H-D-Phe-Pip-Arg-pNA-2HCI also referred to as D-phenylalanyl-L-pipecolyl-L-arginine-4-nitroanilide dihydrochloride) available from Chromogenix, CBS 34.47 (H-D-CHG-But-Arg-pNA) and CBS00.68 both available from Stago, BIOPHEN® CS-01 (81 ) (Tos-Gly-Pro-Arg-pNa. AcOH) and BIOPHEN® CS- 01 (38) (H-D-Phe-Pip-Arg-pNa, 2HCI) both available from HYPHEN BioMed. More preferably, said chromogenic substrate for measuring the anti-factor lla activity is S- 2238™.
The method of the invention may be conducted in solutions of biological samples such as plasma, or in buffer solutions, preferably in buffer solutions.
Buffers may be chosen from any known buffers, in particular those having a pH close to biological media, such as those buffers having a pH comprised between 6.5 and 9. Buffer solutions include in particular tris(hydroxymethyl)aminomethane sodium chloride pH 7.4, tris(hydroxymethyl)aminomethane-EDTA pH 8.4, and tris(hydroxymethyl)aminomethane-NaCI-PEG pH 7.4. They may be prepared according to routine, well-known procedures.
Generally, the method of the invention comprises the following steps:
a) preparing said standard comprising said ULMWH, and preparing said sample,
b) determining the calibration curve for the biological activity of said ULMWH standard,
c) determining the curve for the biological activity of said sample,
d) confirming standard and sample parallelism,
e) determining the biological activity of the ULMWH sample against the calibration curve obtained in step b).
Step a
The ULMWH standard, in a form such as a water for injection solution, may generally be prepared by diluting a given amount of the ULMWH into a given amount of the chosen solution such as buffer solution. The given amounts are advantageously expressed in volumes.
Said ULMWH standard and ULMWH sample are generally processed in the same way, in the same medium, e.g. buffer solution.
The ULMWH sample may be prepared in solution of water for injection, by weighing an appropriate quantity in order to obtain activities in U/mL equivalent to those of the ULMWH standard. The sample is then diluted in the same medium (e.g. buffer solution) as the ULMWH standard.
Generally, there is a loss of weight occurring on drying, due to the absorption of water molecules by the sample; if applicable, this loss is to be determined by methods known in the art, so that the activity may be expressed on dry basis (U/mg).
Steps b and c
A potency (e.g. anti-factor Xa or anti-factor I la activity) expressed in U/mL is assigned to the given amount of the ULMWH, before dilution. A corresponding potency may thus be calculated for the diluted sample, depending on the dilution rate.
Several diluted samples may be prepared, with for each a corresponding potency.
From 4 to 14 calibration points may be performed. Particularly, the method of the invention involves 4 or 14 calibration points depending on whether a routine procedure or experimentation procedure is carried out, respectively. The procedure may be carried out according to the 5-parameters methodology (in particular for the 14 calibration point method) or the parallel lines methodology (in particular for the 4 calibration point method).
The assay for carrying out the method of the invention may be performed by known procedures, such as on a micro-plate reader and dispenser.
The calibration curve may thus be obtained by correlating the calculated potencies with the obtained Optical Density (OD).
The activity in U/mL of the ULMWH sample may be obtained by correlating the activity and the obtained Optical Density (OD), according to the calibration curve obtained with the ULMWH standard.
Generally, the anti-factor Xa activity assigned to the ULMWH standard before dilution is 100 U/mL. Preferably, the points of the calibration curve are comprised between approximately 0.002 and 0.8 U/mL for the anti-factor Xa activity curve in the 14 calibration
points method. The range of the calibration points for the 4 point calibration curve is generally comprised within the above range, such as 0.04-0.07 ± 0.002 U/mL.
Generally, the anti-factor I la activity assigned to the ULMWH standard before dilution is 1 .8 U/mL. Preferably, the points of the calibration curve are comprised between 0.0007 and 0.3 U/mL for the anti-factor I la activity curve in the 14 calibration points method. The range of the calibration points for the 4 point calibration curve is generally comprised within the above range, such as 0.007-0.012 ± 0.001 U/mL.
Step d
For the 14 calibration points method the parallelism between standard and sample is assessed by using the methodology developed by Dunn et al. (Measuring Parallelism, Linearity and Relative Potency in Bioassay and Immunoassay Data, Journal of Biopharmaceutical Statistics, 2005, 15:437-463; The five-parameter logistic: A characterization and comparison with the four-parameter logistic, Analytical Biochemistry, 2005, 343:54-65, both incorporated herein by reference).
For the 4 calibration points method the parallelism between standard and sample is assessed using the statistical analysis described in the chapter 5.3 of Ph. Eur for parallel line methodology. Step e
The biological activity (relative potency) of the ULMWH sample is calculated from the calibration curve obtained with the ULMWH standard.
According to a further object, the present invention also concerns a standard suitable for measuring the in vitro biological activity of an ULMWH sample, which comprises the same ULMWH than the sample to be measured.
Preferably, said standard comprises semuloparin.
Said ULMWH standard is suitable for conducting the method of the invention.
Said ULMWH standard is preferably obtained by diluting a sample of the ULMWH to be measured, which is calibrated by comparison with a recognized standard of LMWH, such as the second international standard of LMWH, currently available. The ULMWH standard is generally assigned an anti-factor Xa activity around 160 U/mg on dry basis and an anti-factor I la of around 2 U/mg on dry basis.
FIGURES
Figures 1 and 2 illustrate the anti-factor Xa activity of bemiparin and semuloparin according to the 14 calibration points procedure.
Figures 3 and 4 illustrate the anti-factor I la activity of bemiparin and semuloparin according to the 14 calibration points procedure.
Figures 5, 6, 7 and 8 illustrate the anti-factor Xa activity and anti-factor lla activity of semuloparin compared with a standard of Low Molecular Mass Heparin for assay Biological Reference Preparation batch 5 (BRP5) according to the 14 calibration points procedure.
Figures 9, 10, 1 1 , 12 illustrate the anti-factor Xa activity and anti-factor lla activity of semuloparin compared with a heparin standard (K5, USP357) according to the 14 calibration points procedure.
Figure 13 and 14 illustrate the anti-factor Xa activity and anti-factor lla activity of semuloparin and a standard comprising semuloparin (3000ET) according to the 4 calibration points procedure.
The following examples are given for representative and non limiting illustration of the invention.
EXAMPLES
I - THE 5-PARAMETERS METHODOLOGY (14-calibration points procedure) Anticoagulant activity of semuloparin was determined in vitro by chromogenic assays measuring its ability to accelerate the inhibition of factor Xa or factor lla by anti- thrombin III.
The curve profile of biological activity of the semuloparin was compared to that of bemiparin, the LMWH reference standard of the European Pharmacopoeia BRP5, and the heparin standard (K5, USP357).
The logistic curves were determined to assess parallelism of the products using the 5-parameters model.
The anti-factor lla and anti-factor Xa activities were measured by assays according to the following protocols:
PREPARATION OF REAGENTS
The reagents used below are all commercially available.
TrisihvdroxymethvDaminomethane sodium chloride buffer solution pH 7.4
Dissolve 6.08 g of tris(hydroxymethyl)aminomethane, 8.77 g of sodium chloride in 500 mL of distilled water. Adjust the pH using hydrochloric acid. Dilute to 1000 mL with distilled water.
Tris(hydroxymethyl)aminomethane sodium chloride PEG buffer solution pH 7.4
Dissolve 6.08 g of tris(hydroxymethyl)aminomethane, 8.77 g of sodium chloride in 500 mL of distilled water. Add 0.5 g of polyethylene glycol 6000. Adjust the pH using hydrochloric acid. Dilute to 1000 mL with distilled water.
Tris(hvdroxymethvDaminomethane-EDTA buffer solution pH 8.4
Dissolve 6.06 g of tris(hydroxymethyl)aminomethane, 2.8 g of ethylenediamine tetracetic acid (EDTA) 10.24 g of sodium chloride in 500 mL of distilled water. Adjust the pH using hydrochloric acid. Dilute to 1000 mL with distilled water.
Acetic acid
Dilute 420 ml of glacial acetic acid to 1000 mL with water.
Antithrombin III solution
Reconstitute antithrombin III as directed by the manufacturer and dilute with tris(hydroxymethyl)aminomethane sodium chloride PEG buffer solution pH 7.4 to 1 lU/mL.
Factor Xa solution, bovine
Reconstitute as directed by the manufacturer and dilute with tris(hydroxymethyl)aminomethane sodium PEG chloride buffer solution pH 7.4 to 1 .8 nKat/mL.
Thrombin solution, human
Reconstitute human thrombin as directed by the manufacturer and dilute with tris(hydroxymethyl)aminomethane sodium chloride PEG buffer solution pH 7.4 to 5 NIH/mL.
Chromogenic substrate for anti-Xa (S2765)
Dissolve N-a-benzyloxycarbonyl-D-arginyl-L-glycyl-L-arginine-4-nitroanilide difiydrocfiloride in water to give a 0.003 M solution. Dilute in tris(fiydroxymetfiyl)aminometfiane-EDTA buffer solution pH 8.4 R to 0.0005 M before use.
Chromogenic substrate for anti-lla (S2238)
Dissolve D-phenylalanyl-L-pipecolyl-L-arginine-4-nitroanilide dihydrochloride in water to give a 0.003 M solution. Dilute before use in titrating in tris(hydroxymethyl)aminomethane-EDTA buffer solution pH 8.4 R to give a 0.0005 M solution.
EQUIPMENT, MATERIALS, ANALYTICAL CONDITIONS
Equipment is given indicatively - any other equipment validated for this method can be used.
PACKARD MULTIPROBE II STD: Automated liquid handling system (dilution robot).
MR 7000 or DIAS from LABSYSTEM: Microplate reader, incubator and dispenser. PREPARATION OF SOLUTIONS TO BE ASSAYED
Prepare the semuloparin solutions in order to obtain activities in U/mL equivalent to those of the standard currently used (stock solution in water for injection).
If possible, determine loss on drying while the substances are being weighed, in order to express the activity on the dried basis; if not, express it on the as-is basis.
The following dilutions are performed in the Tris-NaCI pH 7.4 buffer.
PREPARATION OF STANDARDS
Semuloparin, the LMWH standard of the European Pharmacopoeia (BRP5), the heparin standard (K5 USP 357) and the semuloparin reference standard (3000ET) are diluted in the same way, and the ranges in U/mL or UI/mL are given in Tables 1 and 2.
Anti-factor Xa activity
Table 1 - Standard range for anti-factor Xa activity (geometric progression of 1 .6)
Activity in U/mL
first calibration point (S1 ) 0.8 last calibration point (S14) 0.002 Anti-factor Ma activity
Table 2- Standard range for anti-factor lla activity (geometric progression of 1 .6)
Activity in U/mL
first calibration point (S1 ) 0.3 last calibration point (S14) 0.0007
ASSAY
The assay was performed using MR7000 or DIAS (dynex) from LABSYSTEM. ANTI-FACTOR Xa ACTIVITY:
After dispensing 20 μΙ_ of solution to analyze, add 20 μΙ_ of Antithrombin III (ATIII), mix and equilibrate for 1 minute at 37°C or at room temperature and add 40 μΙ_ of bovine factor Xa solution. Incubate 1 minute and add 100 μΙ of chromogenic substrate for anti- FXa (S2765). Stop the reaction after 4 minutes by adding 100 μΙ_ of acetic acid. Measure the absorbance at 405 nm.
ANTI-FACTOR lla ACTIVITY:
After dispensing 20 μΙ_ of solution to analyse, add 20 μΙ_ of ATIII, mix and equilibrate for 1 minute at 37°C or at room temperature and add 40 μΙ_ of human thrombin solution. Incubate 1 minute and add 100 μΙ_ of chromogenic substrate for anti-Flla (S2238). Stop the reaction after 4 minutes by adding 100 μΙ_ of acetic acid. Measure the absorbance at 405 nm.
RESULTS INTERPRETATION
Establish the parallelism using the 5-parameters methodology, described by Dunn et al. (ibid).
The anti-factor Xa and anti-factor lla experiments were performed 6 times (mean of 6 curves).
According to the above procedures, semuloparin was compared to a lower, second-generation LMWH (bemiparin - fig. 1 to 4), a LMWH international standard (BRP5 - fig. 5-8) and a heparin reference standard (K5, USP357 - fig. 9-12). 1 . Comparison of bemiparin and semuloparin
Results are illustrated in Figures 1 and 2 for the anti-factor Xa activity and in figures 3 and 4 for the anti-factor lla activity.
According the methology of Dunn et al (ibid) the statistical analysis and parallelism were assessed. Anti-FXa concentration response curves indicated that the constrainted and the unconstrainted models were equivalent, suggesting that the curves are similar and then the in vitro anti-FXa behavior of bemiparin and semuloparin could be considered as equivalent. In contrast anti-Flla concentration response curves showed that the constrainted and the unconstrainted models were not equivalent, suggesting that the curves are not similar, reflecting a non parallelism between the 2 curves. Finally, the in vitro anti-Flla behavior of bemiparin and semuloparin could not be considered as equivalent (see also Jeske W.P. et al., "A common standard is inappropriate for determining the potency of ultra low molecular weight heparins such as semuloparin and bemiparin", Thromb. Res., 201 1 , 128(4), 361 -367). 2. Comparison of Low Molecular Mass Heparin standard (BRP5) and semuloparin
Results are illustrated in Figures 5 and 7 for the anti-factor Xa activity and in figures 6 and 8 for the anti-factor lla activity
Using the same statistical methodology, it was showed that for both anti FXa and anti-Flla the curves are not similar between the LMWH (BRP5) standard and semuloparin, suggesting both the in vitro anti FXa and anti Fl la behavior of LMWH and semuloparin could not be considered as equivalent.
3. Comparison of heparin standard (K5, USP357) and semuloparin
Results are illustrated in Figures 9 and 1 1 for the anti-factor Xa activity and in figures 10 and 12 for the anti-factor lla activity.
Same conclusion as above, both the in vitro anti FXa and anti Fl la behavior of heparin standard and semuloparin could not be considered as equivalent, after the assessment of the parallelism using the 14 points calibration methodology.
It is apparent that semuloparin can not be compared with either bemiparin, the
LMWH standard or the heparin standard.
In conclusion, the above results using 14 calibration points methodology clearly and unexpectedly show that semuloparin could not be validly compared with the usual LMWH standard.
II - THE 4-CALIBRATION POINTS METHODOLOGY
This method may be used as a routine procedure. Anticoagulant activity of semuloparin was determined in vitro by chromogenic assays. The methods were adapted from those recommended by the European Pharmacopoeia for low molecular weight heparin (LMWH).
The ability of semuloparin to accelerate the inhibition of factor Xa or factor lla by anti-thrombin III was measured.
The biological activity of the sample was compared to that of a well characterised in-house semuloparin reference standard.
The potency of semuloparin was expressed by comparison with the standard preparation, using the usual statistical methods for parallel-line assays (§5.3 of the European Pharmacopoeia).
The anti-factor lla and anti-factor Xa activities were measured by assays according to the following protocols:
PREPARATION OF REAGENTS
Same specifications as described above under the 14-calibration points procedure.
PREPARATION OF STANDARDS
The standard and test samples are processed in the same way. Any dilution method is appropriate, provided that the ranges in U/mL are identical to those given in Table 3 and Table 4.
Anti-factor Xa activity
Table 3 - Standard range for anti-factor Xa activity (geometric progression of 1 .2)
Activity in U/mL
1 st calibration point (S1 ) 0.04
4th calibration point (S4) 0.07
Anti-factor Ma activity
Table 4- Standard range for anti-factor lla activity (geometric progression of 1 .2)
Activity in U/mL
1 st calibration point (S1 ) 0.007
4th calibration point (S4) 0.012
ASSAY
The assay is performed using MR7000 or DIAS from LABSYSTEM, ANTI-FACTOR Xa ACTIVITY:
After dispensing 20 μΙ_ of solution to analyse, add 20 μΙ_ of ATIII, mix and equilibrate at 37°C or at room temperature for 1 minute and add 40 μΙ of bovine factor Xa solution. Incubate 1 minute and add 100 μΙ of chromogenic substrate for anti-FXa (S2765). Stop the reaction after 4 minutes by adding 100 μΙ of acetic acid. Measure the absorbance at 405 nm.
Calculate the regression of the absorbance on log concentrations of the test and standard solutions and calculate the potency using the usual statistical methods for parallel-line assays (§5.3 European Pharmacopoeia) for each serie. For each serie the validity criteria must be fulfilled and the final potency is calculated in Units of anti-factor Xa activity per milliliter (or per milligram) by an arithmetic mean of the two preparations.
ANTI-FACTOR lla ACTIVITY:
After dispensing 20 μΙ_ of solution to analyse, add 20 μΙ_ of ATIII, mix and equilibrate at 37 °C or at room temperature for 1 minute and add 40 μΙ of human thrombin solution. Incubate 1 minute and add 100 μΙ of chromogenic substrate for anti-Flla (S2238). Stop the reaction after 4 minutes by adding 100 μΙ of acetic acid. Measure the absorbance at 405 nm.
Calculate the regression of the absorbance on log concentrations of the test and standard solutions and calculate the potency using the usual statistical methods for parallel-line assays (§5.3 European Pharmacopoeia) for each serie. For each serie the validity criteria must be fulfilled and the final potency is calculated in Units of anti-factor lla activity per milliliter (or per milligram) by an arithmetic mean of the three preparations.
RESULTS INTERPRETATION
For each run, the analysis of variance must be valid and the C test less than 1 .2. This may be done by the parallel line method according to the European pharmacopoeia or according to the Dunn et al. (see above) method.
EXPRESSION OF RESULTS
The loss on drying is measured according to Chapter 2.2.32 of the European
Pharmacopoeia.
According to the above procedures, semuloparin was compared to a standard comprising semuloparin (3000ET).
Comparison of semuloparin with a standard comprising semuloparin (3000ET)
Results are illustrated in Figures 13 and 14.
According to the usual statistical methods for parallel line assays (§5.3 European Pharmacopoeia), the analysis is considered valid and both products are considered to have the same in vitro behaviour for the anti-factor Xa and anti-factor I la activity.
In conclusion, the above results clearly show that semuloparin can be validly compared with a standard comprising semuloparin. III - IN VITRO MEASUREMENT OF THE BIOLOGICAL ACTIVITY OF ULMWH
SAMPLES
The anti-FXa and anti-Flla activities of semuloparin containing samples were determined according to the 4-points calibration methodology disclosed above, using a standard comprising semuloparin.
Results are summarized in the table below:
NB : the loss on drying was measured at 70 °C according to process d) of Chapter 2.2.32 of the European Pharmacopoeia
Claims
1 . A method for the in vitro measurement of the biological activity of an Ultra Low Molecular Weight Heparin (ULMWH) sample, wherein said method is carried out relative to a standard comprising an ULMWH.
2. Method according to claim 1 wherein said standard comprises semuloparin.
3. Method according to claim 1 or 2, wherein said ULMWH is semuloparin.
4. Method according to claim 1 , 2 or 3 wherein said standard comprises the same ULMWH as the ULMWH sample.
5. Method according to anyone of the preceding claims comprising the measurement of the anti-factor Xa activity of said ULMWH sample.
6. Method according to anyone of the preceding claims, for the measurement of the anti-factor I la activity of said ULMWH sample.
7. Method according to anyone of the preceding claims, wherein said method is an amidolytic assay on a chromogenic substrate.
8. Method according to claims 5 and 7, wherein said chromogenic substrate is chosen from the group consisting in S-2222™ (Bz-lle-Glu(g-OR)-Gly-Arg-pNA-HCI); S-
2765™ (N-a-Z-D-Arg-Gly-Arg-pNA 2HCI also referred to as N-a-benzyloxycarbonyl-D- arginyl-L-glycyl-L-arginine-4-nitroanilide dihydrochloride); CBS 31 .39 (CH3-S02-D-l-eu-Gly- Arg-pNA) available from Stago; BIOPHEN® CS-1 1 (22) (Mixture (50%-50%) of Bz-lle-Glu (YOCH3)-Gly-Arg-pNa (form 1 ) and Bz-lle-Glu (yOH)Gly-Arg-pNa (form 2)); BIOPHEN® CS-1 1 (32) (Suc-lle-Gly (γ Pip)Gly-Arg-pNa, HCI); BIOPHEN® CS - 1 1 (65) (D-Arg-Gly- Arg-pNA, 2HCI).
9. Method according to claims 6 and 7, wherein said chromogenic substrate is chosen from the group consisting in S-2238™ (H-D-Phe-Pip-Arg-pNA-2HCI also referred to as D-phenylalanyl-L-pipecolyl-L-arginine-4-nitroanilide dihydrochloride); CBS 34.47 (H-D-CHG-But-Arg-pNA); CBS00.68; BIOPHEN® CS-01 (81 ) (Tos-Gly-Pro-Arg- pNa. AcOH); BIOPHEN® CS-01 (38) (H-D-Phe-Pip-Arg-pNa, 2HCI).
10. Method according to anyone of the preceding claims, wherein the sample is in buffer solution.
1 1 . Method according to anyone of the preceding claims, comprising the following steps :
a) preparing said standard comprising said ULMWH, and preparing said sample b) determining the calibration curve for the biological activity of said ULMWH standard
c) determining the curve for the biological activity of said sample,
d) confirming standard and sample parallelism
e) determining the biological activity of the ULMWH sample against the calibration curve obtained in step b).
12. Method according to claim 1 1 wherein the calibration curve is obtained from 14 calibration points.
13. Method according to claim 1 1 wherein the calibration curve is obtained from 4 calibration points.
14. Standard suitable for measuring the in vitro biological activity of an ULMWH sample, which comprises an ULMWH.
15. Standard according to claim 14 wherein said standard comprises the same ULMWH as the sample to be measured.
16. Standard according to claim 14 or 15, comprising semuloparin.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10306342.6 | 2010-12-02 | ||
EP10306342 | 2010-12-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2012072799A1 true WO2012072799A1 (en) | 2012-06-07 |
Family
ID=43797687
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2011/071633 WO2012072799A1 (en) | 2010-12-02 | 2011-12-02 | New methods for the in vitro measurement of the biological activity of an ultra low molecular weight heparin sample |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2012072799A1 (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1070503A1 (en) * | 1999-07-23 | 2001-01-24 | Laboratorios Farmaceuticos Rovi, S.A. | Compositions comprising very low molecular weight Heparin |
WO2004033503A1 (en) | 2002-10-10 | 2004-04-22 | Aventis Pharma S.A. | Heparin-derived polysaccharide mixtures, preparation thereof and pharmaceutical compositions containing same |
WO2005010051A2 (en) | 2003-07-24 | 2005-02-03 | Aventis Pharma S.A. | Heparin-derived oligosaccharide mixtures, preparation thereof and pharmaceutical compositions containing said mixtures |
EP2233145A1 (en) * | 2009-03-19 | 2010-09-29 | Sanofi-Aventis | A dose of AVE5026 for the treatment of venous thromboembolism in patients with severe renal impairment |
-
2011
- 2011-12-02 WO PCT/EP2011/071633 patent/WO2012072799A1/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1070503A1 (en) * | 1999-07-23 | 2001-01-24 | Laboratorios Farmaceuticos Rovi, S.A. | Compositions comprising very low molecular weight Heparin |
WO2004033503A1 (en) | 2002-10-10 | 2004-04-22 | Aventis Pharma S.A. | Heparin-derived polysaccharide mixtures, preparation thereof and pharmaceutical compositions containing same |
WO2005010051A2 (en) | 2003-07-24 | 2005-02-03 | Aventis Pharma S.A. | Heparin-derived oligosaccharide mixtures, preparation thereof and pharmaceutical compositions containing said mixtures |
EP2233145A1 (en) * | 2009-03-19 | 2010-09-29 | Sanofi-Aventis | A dose of AVE5026 for the treatment of venous thromboembolism in patients with severe renal impairment |
Non-Patent Citations (10)
Title |
---|
"The five-parameter logistic: A characterization and comparison with the four-parameter logistic", ANALYTICAL BIOCHEMISTRY, vol. 343, 2005, pages 54 - 65 |
ANDERSON L. O. ET AL., THROMBOSIS RESEARCH, vol. 15, 1979, pages 531 - 541 |
C. VISKOV ET AL., JOURNAL OF THROMBOSIS AND HAEMOSTASIS, vol. 7, 2009, pages 1143 - 1151 |
DUBRUC CATHERINE ET AL: "Pharmacokinetics of a New, Ultra-Low Molecular Weight Heparin, Semuloparin (AVE5026), in Healthy Subjects. Results From the First Phase I Studies", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 14, no. 22, 1 November 2009 (2009-11-01), pages 443, XP009137841, ISSN: 0006-4971 * |
DUNN ET AL.: "Measuring Parallelism, Linearity and Relative Potency in Bioassay and Immunoassay Data", JOURNAL OF BIOPHARMACEUTICAL STATISTICS, vol. 15, 2005, pages 437 - 463 |
GÓMEZ-OUTES ANTONIO ET AL: "New parenteral anticoagulants in development", THERAPEUTIC ADVANCES IN CARDIOVASCULAR DISEASE, SAGE PUBLICATIONS LTD, GB, vol. 5, no. 1, 2 November 2010 (2010-11-02), pages 33 - 59, XP009146821, ISSN: 1753-9447, [retrieved on 20101102], DOI: DOI:10.1177/1753944710387808 * |
JESKE W.P. ET AL.: "A common standard is inappropriate for determining the potency of ultra low molecular weight heparins such as semuloparin and bemiparin", THROMB. RES., vol. 128, no. 4, 2011, pages 361 - 367, XP028391145, DOI: doi:10.1016/j.thromres.2011.03.001 |
LASSEN M R ET AL: "AVE5026, a new hemisynthetic ultra-low-molecular-weight heparin for the prevention of venous thromboembolism in patients after total knee replacement surgery - TREK: A dose-ranging study", JOURNAL OF THROMBOSIS AND HAEMOSTASIS, BLACKWELL PUBLISHING, OXFORD, GB, vol. 7, no. 4, 24 January 2009 (2009-01-24), pages 566 - 572, XP002533591, ISSN: 1538-7933, DOI: DOI:10.1111/J.1538-7836.2009.03301.X * |
TEIEN ET AL., THROMBOSIS RESEARCH, vol. 10, 1977, pages 399 - 410 |
VISKOV C ET AL: "Description of the chemical and pharmacological characteristics of a new hemisynthetic ultra-low-molecular-weight heparin, AVE5026", JOURNAL OF THROMBOSIS AND HAEMOSTASIS, WILEY INTERSCIENCE, ENGLAND, vol. 7, no. 7, 1 July 2009 (2009-07-01), pages 1143 - 1151, XP002545508, ISSN: 1538-7836, DOI: DOI:10.1111/J.1538-7836.2009.03447.X * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1319183B1 (en) | Methods and products related to low molecular weight heparin | |
Yu et al. | Preparation and anticoagulant activity of the phosphosulfomannan PI-88 | |
Schroeder et al. | Protamine neutralisation of low molecular weight heparins and their oligosaccharide components | |
Brustkern et al. | Characterization of currently marketed heparin products: reversed-phase ion-pairing liquid chromatography mass spectrometry of heparin digests | |
EP2864496B2 (en) | Euglobulin-based method for determining the biological activity of defibrotide | |
US20160310521A1 (en) | Polysaccharides comprising two antithrombin iii-binding sites, preparation thereof and use thereof as antithrombotic medicaments | |
DE112006003476T5 (en) | Methods and systems for detecting and quantifying indirect thrombin inhibitors | |
US20120009686A1 (en) | Method of measuring the concentration of a glycosaminoglycan anticoagulant | |
DE102005038418A1 (en) | Factor Xa-based heparin assay using a heparin-modifying component | |
Barrowcliffe et al. | The anticoagulant activity of heparin: measurement and relationship to chemical structure | |
Ye et al. | Characteristics of low molecular weight heparin production by an ultrafiltration membrane bioreactor using maltose binding protein fused heparinase I | |
EP3018481B1 (en) | Blood coagulation time prolonging agent | |
Suzuki et al. | The establishment and validation of efficient assays for anti-IIa and anti-Xa activities of heparin sodium and heparin calcium | |
Handeland et al. | Assay of unfractionated and LMW heparin with chromogenic substrates: twin methods with factor Xa and thrombin | |
WO2012072799A1 (en) | New methods for the in vitro measurement of the biological activity of an ultra low molecular weight heparin sample | |
Carnachan et al. | Heparan sulfate identification and characterisation: Method II. Enzymatic depolymerisation and SAX-HPLC analysis to determine disaccharide composition | |
Lin et al. | Plasma contact activation by a fucosylated chondroitin sulfate and its structure–activity relationship study | |
Houbouyan et al. | Validation protocol of analytical hemostasis systems: measurement of anti-Xa activity of low-molecular-weight heparins | |
Wages et al. | Structural characterization and functional effects of a circulating heparan sulfate in a patient with hepatocellular carcinoma | |
Houbouyan et al. | Inhibition of thrombin generation by heparin and LMW heparins: a comparison of chromogenic and clotting methods | |
Zhang et al. | Quantitative analysis of antithrombin III binding site in low molecular weight heparins by exhausetive heparinases digestion and capillary electrophoresis | |
CN116875657B (en) | Quantitative analysis method for glycoprotein of recombinant batroxobin | |
CN105203479A (en) | Method for detecting potency of heparin or heparin with low molecular weight through a coagulometer | |
CN115290581B (en) | Anti-Xa assay kit and method for detecting anti-Xa activity | |
Münstedt et al. | Evaluation of an amidolytic test for comparative calibration of HMW-and LMW-heparins |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 11793406 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 11793406 Country of ref document: EP Kind code of ref document: A1 |