WO2012071405A3 - Kits and assays for amplification of expressed salmonella genes from blood - Google Patents

Kits and assays for amplification of expressed salmonella genes from blood Download PDF

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Publication number
WO2012071405A3
WO2012071405A3 PCT/US2011/061832 US2011061832W WO2012071405A3 WO 2012071405 A3 WO2012071405 A3 WO 2012071405A3 US 2011061832 W US2011061832 W US 2011061832W WO 2012071405 A3 WO2012071405 A3 WO 2012071405A3
Authority
WO
WIPO (PCT)
Prior art keywords
kits
expressed
assays
amplification
blood
Prior art date
Application number
PCT/US2011/061832
Other languages
French (fr)
Other versions
WO2012071405A2 (en
Inventor
Edward T. Ryan
Richelle Charles
Alaullah Sheikh
Firdausi Qadri
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Publication of WO2012071405A2 publication Critical patent/WO2012071405A2/en
Publication of WO2012071405A3 publication Critical patent/WO2012071405A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention described relates to methods of and kits for detecting Salmonella enterica serotypes Typhi and Paratyphi A transcripts that are highly expressed in human blood. The detection is performed from human blood samples in vitro.
PCT/US2011/061832 2010-11-23 2011-11-22 Kits and assays for amplification of expressed salmonella genes from blood WO2012071405A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41663910P 2010-11-23 2010-11-23
US61/416,639 2010-11-23

Publications (2)

Publication Number Publication Date
WO2012071405A2 WO2012071405A2 (en) 2012-05-31
WO2012071405A3 true WO2012071405A3 (en) 2012-11-15

Family

ID=46146389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/061832 WO2012071405A2 (en) 2010-11-23 2011-11-22 Kits and assays for amplification of expressed salmonella genes from blood

Country Status (1)

Country Link
WO (1) WO2012071405A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103571943B (en) * 2013-08-23 2016-05-11 四川农业大学 The nucleic acid detection method of a kind of salmonella typhimurium
CN109182240B (en) * 2018-09-18 2021-06-25 绿康生化股份有限公司 Application of bacillus licheniformis DW2 delta phoP in bacitracin production
CN112538543B (en) * 2020-12-16 2022-09-27 山东农业大学 Specific primer for detecting salmonella and kit thereof
CN113388539B (en) * 2020-12-30 2022-05-20 广东省微生物研究所（广东省微生物分析检测中心） Salmonella standard strain containing specific molecular target and detection and application thereof
CN112813151A (en) * 2021-03-05 2021-05-18 为朔医学数据科技（北京）有限公司 Whole blood direct fluorescence PCR detection method and application of whole blood sample treatment fluid in preparation of gene detection kit

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CENCIARINI-BORDE, C. ET AL.: "Nucleic acids as viability markers for bacteria detection using molecular tools", FUTURE MICROBIOLOGY., vol. 4, no. 1, February 2009 (2009-02-01), pages 45 - 64, XP008119055, DOI: doi:10.2217/17460913.4.1.45 *
D' SOUZA, D. H. ET AL.: "Real-time reverse-transcriptase polymerase chain reaction for the rapid detection of Salmonella using invA primers", FOODBORNE PATHOGENS AND DISEASE., vol. 6, no. 9, November 2009 (2009-11-01), pages 1097 - 1106 *
KUMAR, A. ET AL.: "Quick PCR based diagnosis of typhoid using specific genetic markers", BIOTECHNOLOGY LETTERS., vol. 32, no. 5, 6 February 2010 (2010-02-06), pages 707 - 712, XP019787091 *
MORIN, N. ET AL.: "Reverse transcription-multiplex PCR Assay for simultaneous detection of Escherichia coli 0157:H7, Vibrio cholerae 01, and Salmonella Typhi", CLINICAL CHEMISTRY, vol. 50, no. 11, 13 September 2004 (2004-09-13), pages 2037 - 2044 *
SHEIKH, A. ET AL.: "Analysis of Salmonella enterica serotype paratyphi A gene expression in the blood of bacteremic patients in Bangladesh", PLOS NEGLECTED TROPICAL DISEASE., vol. 4, no. 12, 7 December 2010 (2010-12-07), pages E908 *

Also Published As

Publication number Publication date
WO2012071405A2 (en) 2012-05-31

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