WO2012071039A8 - Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof - Google Patents

Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof Download PDF

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Publication number
WO2012071039A8
WO2012071039A8 PCT/US2010/058002 US2010058002W WO2012071039A8 WO 2012071039 A8 WO2012071039 A8 WO 2012071039A8 US 2010058002 W US2010058002 W US 2010058002W WO 2012071039 A8 WO2012071039 A8 WO 2012071039A8
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WO
WIPO (PCT)
Prior art keywords
compositions
event
identification
detection
methods
Prior art date
Application number
PCT/US2010/058002
Other languages
French (fr)
Other versions
WO2012071039A1 (en
Inventor
Lomas Tulsieram
Yongping Zhang
Jayantilal Devabhai Patel
David George Charne
Wenpin Chen
Ferdinand Gerard Thoonen
Chadwick Bruce Koscielny
Zhongsen Li
Original Assignee
Pioneer Hi-Bred International, Inc.
E.I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc., E.I. Du Pont De Nemours And Company filed Critical Pioneer Hi-Bred International, Inc.
Priority to PCT/US2010/058002 priority Critical patent/WO2012071039A1/en
Priority to CA2818918A priority patent/CA2818918A1/en
Publication of WO2012071039A1 publication Critical patent/WO2012071039A1/en
Publication of WO2012071039A8 publication Critical patent/WO2012071039A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Abstract

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-061061-7 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-061061-7 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of events provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the events are provided.
PCT/US2010/058002 2010-11-24 2010-11-24 Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof WO2012071039A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/US2010/058002 WO2012071039A1 (en) 2010-11-24 2010-11-24 Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof
CA2818918A CA2818918A1 (en) 2010-11-24 2010-11-24 Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2010/058002 WO2012071039A1 (en) 2010-11-24 2010-11-24 Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof

Publications (2)

Publication Number Publication Date
WO2012071039A1 WO2012071039A1 (en) 2012-05-31
WO2012071039A8 true WO2012071039A8 (en) 2012-08-23

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CA (1) CA2818918A1 (en)
WO (1) WO2012071039A1 (en)

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