WO2012070795A3 - Ipomoea batatas-derived ibor-ins gene mutation, and use thereof - Google Patents

Ipomoea batatas-derived ibor-ins gene mutation, and use thereof Download PDF

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Publication number
WO2012070795A3
WO2012070795A3 PCT/KR2011/008671 KR2011008671W WO2012070795A3 WO 2012070795 A3 WO2012070795 A3 WO 2012070795A3 KR 2011008671 W KR2011008671 W KR 2011008671W WO 2012070795 A3 WO2012070795 A3 WO 2012070795A3
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WO
WIPO (PCT)
Prior art keywords
derived
ipomoea batatas
ibor
gene mutation
transformed plant
Prior art date
Application number
PCT/KR2011/008671
Other languages
French (fr)
Korean (ko)
Other versions
WO2012070795A2 (en
WO2012070795A9 (en
Inventor
곽상수
이행순
안영옥
정재철
김선하
Original Assignee
한국생명공학연구원
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Publication date
Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Priority to CN2011800561532A priority Critical patent/CN103269578A/en
Publication of WO2012070795A2 publication Critical patent/WO2012070795A2/en
Publication of WO2012070795A3 publication Critical patent/WO2012070795A3/en
Publication of WO2012070795A9 publication Critical patent/WO2012070795A9/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a method for producing: an IbOr-Ins gene mutation in which a certain sequence is artificially inserted into an Ipomoea batatas-derived IbOrange gene; a recombinant vector including the gene mutation; a host cell mutated by the recombinant vector; and a transformed plant body that has an improved carotenoid content and resistance to saline stress by means of the mutation of the recombinant vector in a plant cell. The present invention also relates to a method for producing a transformed plant body having increased resistance to saline stress using the transformed plant body or seeds thereof produced by the above method and having an improved carotenoid content and resistance to saline stress, and using an Ipomoea batatas-derived IbOrange gene. The invention also relates to a transformed plant body produced by the above method and having increased resistance to saline stress, and to the seeds thereof.
PCT/KR2011/008671 2010-11-22 2011-11-14 Ipomoea batatas-derived ibor-ins gene mutation, and use thereof WO2012070795A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011800561532A CN103269578A (en) 2010-11-22 2011-11-14 Ibor-Ins gene mutant from ipomoea batatas and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100115965A KR101281071B1 (en) 2010-11-22 2010-11-22 IbOr-Ins gene mutant from Ipomoea batatas and uses thereof
KR10-2010-0115965 2010-11-22

Publications (3)

Publication Number Publication Date
WO2012070795A2 WO2012070795A2 (en) 2012-05-31
WO2012070795A3 true WO2012070795A3 (en) 2012-10-11
WO2012070795A9 WO2012070795A9 (en) 2012-11-08

Family

ID=46146247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/008671 WO2012070795A2 (en) 2010-11-22 2011-11-14 Ipomoea batatas-derived ibor-ins gene mutation, and use thereof

Country Status (3)

Country Link
KR (1) KR101281071B1 (en)
CN (1) CN103269578A (en)
WO (1) WO2012070795A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101359308B1 (en) * 2011-08-05 2014-02-10 한국생명공학연구원 Method for producing transgenic sweet potato plant accumulating carotenoid and anthocyanin at high level and the plant thereof
KR101478112B1 (en) * 2012-10-12 2014-12-31 한국생명공학연구원 IbOr-Ins gene increasing black rot resistance from Ipomoea batatas and uses thereof
KR102110870B1 (en) * 2017-12-19 2020-05-15 한국생명공학연구원 IbOr-R96H mutant from Ipomoea batatas and uses thereof
CN108834803A (en) * 2018-07-12 2018-11-20 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) A method of induction sweet potato buddings
KR102525540B1 (en) * 2021-03-05 2023-04-25 한국생명공학연구원 LCYB2 mutant from Daucus carota and uses thereof
CN117164686B (en) * 2022-05-11 2024-05-31 中国农业大学 Stress-resistance related protein IbRCD1, related biological material and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100990330B1 (en) * 2008-06-20 2010-10-29 한국생명공학연구원 IbOrange gene involved in carotenoid accumulation from Ipomoea batatas

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8138391B1 (en) 2005-12-07 2012-03-20 The United States Of America As Represented By The Secretary Of Agriculture Or gene and its use in manipulating carotenoid content and composition in plants and other organisms
KR100813284B1 (en) 2006-04-28 2008-03-13 제주대학교 산학협력단 Transgenic rape plants transformed phytoene synthase gene of a tangerine
KR20100100097A (en) * 2009-03-05 2010-09-15 화이젠 주식회사 Mus1 gene from sweetpotato and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100990330B1 (en) * 2008-06-20 2010-10-29 한국생명공학연구원 IbOrange gene involved in carotenoid accumulation from Ipomoea batatas

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HIRONORI MANO ET AL.: "Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato", PLANT PHYSIOLOGY, vol. 143, 2007, pages 1252 - 1268, XP003019791, DOI: doi:10.1104/pp.106.094425 *
MYOUNG DUCK KIM ET AL.: "Selection of transgenic sweetpotato plants expressing 2-Cys peroxiredoxin with enhanced tolerance to oxidative stress", JOURNAL OF BIOTECHNOLOGY, vol. 36, no. 1, 2009, pages 75 - 80 *
SALGADO-GARCIGLIA ET AL.: "NaCl-resistant variant cells isolated from sweet potato cell suspensions", PLANT CELL TISSUE ORGAN CULTURE, vol. 5, 1985, pages 3 - 12 *
SHAN LU ET AL.: "The Cauliflower Or Gene Encodes a DnaJ Cysteine-Rich Doma in-Containing Protein That Mediates High Levels of b-Carotene Accumulation", THE PLANT CELL, vol. 18, 2006, pages 3594 - 3605 *

Also Published As

Publication number Publication date
KR20120054712A (en) 2012-05-31
WO2012070795A2 (en) 2012-05-31
WO2012070795A9 (en) 2012-11-08
CN103269578A (en) 2013-08-28
KR101281071B1 (en) 2013-07-09

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