WO2012032544A2 - Sphaeranthus indicus derived ingredients and their compositions for enhacing physical performance and energy levels - Google Patents
Sphaeranthus indicus derived ingredients and their compositions for enhacing physical performance and energy levels Download PDFInfo
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- WO2012032544A2 WO2012032544A2 PCT/IN2011/000612 IN2011000612W WO2012032544A2 WO 2012032544 A2 WO2012032544 A2 WO 2012032544A2 IN 2011000612 W IN2011000612 W IN 2011000612W WO 2012032544 A2 WO2012032544 A2 WO 2012032544A2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/38—Clusiaceae, Hypericaceae or Guttiferae (Hypericum or Mangosteen family), e.g. common St. Johnswort
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
Definitions
- the invention discloses a herbal ingredient selected from extracts and fractions standardized to at least one frullanolide/eudesmanoid sesquiterpene compound(s) derived from Sphaeranthus indicus or their composition(s) as natural energy enhancer to provide an onset and steady maintenance of energy and mental alertness in a mammal.
- the invention further relates to food ingredient formulations such as beverages, dietary ingredient formulation, snacks and energy drinks containing the above said herbal ingredient or its composition(s) for onset and steady maintenance of energy and mental alertness such as enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better physical and mental health.
- food ingredient formulations such as beverages, dietary ingredient formulation, snacks and energy drinks containing the above said herbal ingredient or its composition(s) for onset and steady maintenance of energy and mental alertness such as enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better physical and mental health.
- Dietary supplements are used by physically active people or weak people to increase their physical performance, physical fitness, improve their health, or reduce the potentially negative consequences of physical activity such as injury and chronic fatigue, or suppressed immune function.
- the present invention addresses the existing need in the art by providing an ingredient(s) or composition(s) that provides energy and/or mental alertness.
- the present invention provides ingredient(s) and/or composition(s) capable of increasing energy levels for an extended period of time in a mammal to enhance both energy levels and mental alertness and thus solves these needs.
- the invention discloses a herbal ingredient selected from extracts and fractions standardized to at least one frullanolide/eudesmanoid sesquiterpene compound(s) derived from Sphaeranthus indicus or their composition(s) for onset and steady maintenance of energy and mental alertness.
- the sphaeranthus derived ingredient and its compositions are useful for enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better physical and mental health in a mammal.
- the invention in another embodiment, relates to the preparation of dietary supplements, food ingredient formulations such as beverages, snacks and energy drinks containing the above said ingredient(s) or its composition(s) for onset and steady maintenance of energy and mental alertness.
- the formulation is useful for enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better physical and mental health in a mammal.
- the extracts or fractions are standardized to 7- hydroxyfrullanolide, its analogs or their compositions for onset and steady maintenance of energy; mental alertness and for enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better mental health in warm blooded animals in need thereof.
- compositions described above comprise at least one herbal ingredient selected from extract or fraction standardized to at least one frullanolide/eudesmanoid sesquiterpene compound(s) derived from Sphaeranthus indicus in combination with at least one ingredient selected from extract(s), fraction(s), active compound(s), phytochemical(s), derived from plant(s), animal(s) or microorganisms with proven therapeutic health benefits; pharmaceutically or dietetically acceptable agents, active ingredients, vitamins, amino acids and minerals.
- one or more herbal ingredients derived from Sphaeranthus indicus or their compositions are useful for onset and steady maintenance of energy; mental alertness and for enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better mental health in warm blooded animals in need thereof.
- the invention provides a method of increasing natural energy to provide an onset and steady maintenance of energy and mental alertness in a mammal, wherein the method comprises supplementing or treating the said mammal with at least one herbal ingredient selected from the extracts, fractions, compounds; extracts or fractions standardized to active compounds derived from Sphaeranthus indicus or their compositions.
- Figure II Figure shows Percentage of energy endurance capacity of LI12508. The bars represent percentage resting time, moving slow time and moving fast times in seconds.
- the phrase 'herbal ingredient' OR 'herbal ingredients' OR 'said herbal ingredients' used in the specification and claims of the present invention refer to the extract(s), fraction(s); extract(s) or fraction(s) standardized to at least one frullanolide/eudesmanoid sesquiterpene compound(s) derived from Sphaeranthus indicus or their composition(s).
- the phrase 'herbal ingredient' OR 'herbal ingredients' is used interchangeably through out the specification and the same may be appreciated as such by the person skilled in the art.
- compositions' OR 'compositions' OR 'mixture' OR 'mixtures' determine the combination of one or more ingredients selected from extract(s), fraction(s) or compound(s) derived from Sphaeranthus indicus or other biologically active ingredients.
- biologically active ingredient' or 'biologically active ingredients' refers to other component(s) used for making compositions and selected from extract(s), fraction(s), active compound(s), phytochemical(s); powder(s) derived from plant(s), animal(s) or microorganisms with proven therapeutic health benefits; pharmaceutically or dietetically acceptable active ingredients, vitamins, amino acids or minerals.
- the phrase 'weak people' means those individuals who may or may not be suffering from a disease and the weakness can be of physical or mental.
- the word 'subject' OR 'subjects' means warm blooded animals, cold blooded animals, birds, horses, human beings or mammals of any sex.
- the participants were randomized into three groups; placebo, LI 12508 group and LI12507F group.
- the participants received either 600 mg/day of LI12508 and 800 mg of LI12507F formulation in two divided doses or two identical placebo capsules and all of them remained on a calorie-controlled diet (2000 Calories/day) and 30 min walking for 8 weeks.
- Various parameters including, physical, anthropomorphic, biochemical and questionnaire based evaluation including quality of life.
- the S. indicus extract (LI 12508) and its formulation LI12507F provided significant reduction in body weight in both the treatment groups and also indicated promise for their potential use in controlling serum triglyceride levels.
- both LI12508 and LI12507F also showed unexpectedly improved quality of life and improved energy, exercise ability and endurance in subjects supplemented with the treatments when compared the placebo treated group. This includes increased mobility, increased walking speed, increased walking distance, lack of fatigue, feeling untired, feeling light and active.
- the intracellular ATP level in the cells treated cells with LI12508 and LI12507F were measured by luminescence based ATP detection assay system, ATPlite (PerkinElmer Inc., Waltham, MA). Briefly, equal number of L6 Rat myocytes was plated in each well of a 96-well cell culture plate and cultured in DMEM supplemented with 10% FBS. The cells were treated with test samples (LI12508 and LI12507F) at 50 pg/mL. Intracellular ATP level was quantitatively estimated from a standard curve plotted with luminescence units vs.
- the Energy Endurance potential of LI12508 following oral administration was further evaluated in male and female Swiss albino mice.
- Swiss albino mice of 20 weeks old were selected and examined for health status and divided into three groups each consisting of 6 animals (3 males and 3 females).
- the control group animals were treated p.o with 0.5% of CMC and treatment groups were treated once a day with the test compound LI12508 at 100 mg/kg or 250 mg/kg body weight for one week.
- the animals were tested for energy endurance with force swimming test using the SMART video tracking system (Panlab S.L.U). Resting time and its percentage, slow moving and its percentage, fast moving and its percentage, total path length of swimming and average velocity were analyzed using SMART video tracking software and the data is summarized in table 2.
- the increase in mean slow swimming time and mean fast swimming time reveals that the animals treated with LI12508 (100 mg/kg and 250 mg/kg) preferred to swim for a longer period of time instead of resting as observed in vehicle treated control animals.
- the increase in mean swimming velocity observed in 250 mg/kg LI12508 treated animals further support the increase in mean distance covered by the animals in the shorter period of time as compared to other two groups. This indicates the animals treated with LI12508 were very active and energetic during 15 minutes forced swimming period and could tend to swim either slow or fast most of the time rather than resting when compared to control group treated with 0.5% CMC.
- the longer distance covered at higher velocity and shorter resting times observed in LI12508 treated animals indicate that LI12508 improves energy and endurance.
- supplementation of Sphaeranthus indicus extract can increase the energy levels and endurance potential.
- Sphaeranthus indicus extract standardized to 5% 7- hydroxyfrullanolide (LI 12508) and its composition in combination with Garcinia mangostana extract standardized to 10% a-mangostin (LI12507F) could be potent natural supplements to provide an onset, steady maintenance of energy, physical endurance and mental alertness in humans and animals.
- the invention discloses a herbal ingredient selected from extracts and fractions standardized to at least one frullanolide/eudesmanoid sesquiterpene compound(s) derived from Sphaeranthus indicus or their composition(s) for onset and steady maintenance of energy and mental alertness.
- the sphaeranthus derived ingredients and its compositions are useful for enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better physical and mental health in a mammal.
- the invention discloses the composition consisting of Sphaeranthus indicus derived herbal ingredient in combination with at least one biologically active ingredient selected from extract of Garcinia mangostana fruit rind, Osmium basilicum, Zingiber officinalis, Tribulus terrestris, vitamins, amino acids, taurine, an extract of Ginkgo biloba, Rhodiola and an extract of guarana, optionally in combination with an excipient, diluent or carrier as a natural energy enhancer to provide an onset and steady maintenance of energy and mental alertness in a warm blooded animal or mammal in need thereof.
- at least one biologically active ingredient selected from extract of Garcinia mangostana fruit rind, Osmium basilicum, Zingiber officinalis, Tribulus terrestris, vitamins, amino acids, taurine, an extract of Ginkgo biloba, Rhodiola and an extract of guarana, optionally in combination with an excipient, diluent or carrier as a
- the invention relates to at least one Sphaeranthus indicus derived herbal ingredient selected from the extracts and fractions standardized to 7- hydroxyfrullanolide or its analogs or mixtures thereof as a natural energy enhancer to provide an enhanced physical or mental performance, onset and steady maintenance of energy and mental alertness in a mammal.
- the invention relates to a composition
- a composition comprising at least one herbal ingredient selected from extract or fraction standardized to at least one frullanolide/eudesmanoid sesquiterpene compound(s) derived from Sphaeranthus indicus or their composition(s) in combination with at least one ingredient selected from the extracts, fractions or compounds derived from Garcinia mangostana, further optionally containing at least one ingredient selected from excipient, diluent, vitamins or amino acids, as a natural energy enhancer for enhancing physical or mental performance, onset and steady maintenance of energy enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better mental health in a mammal.
- the invention provides a composition
- a composition comprising at least one herbal ingredient selected from the extracts, fractions or compounds derived from Sphaeranthus indicus in combination with at least one ingredient selected from the extracts, fractions or compounds derived from Garcinia mangostana, optionally containing at least ingredient selected from excipient, diluent, vitamins or amino acids, as a natural energy enhancer for enhancing physical or mental performance, onset and steady maintenance of energy and mental alertness in a mammal.
- compositions comprising at least one herbal ingredient selected from the extracts or fractions standardized to at least one frullanolide/ eudesmanoid sesquiterpene compound such as 7-hydroxyfrullanolide (1) derived from Sphaeranthus indicus in combination with a Garcinia mangostana extract standardized to cc-mangostin (2) or ⁇ -mangostin (3) and further optionally in combination with suitable excipients as a natural energy enhancer to provide an enhancing physical or mental performance, onset and steady maintenance of energy, mental alertness, for enhancing physical activity, physical fitness, energy levels, stamina levels, circulatory health, blood vessel health or for better mental health in a mammal.
- frullanolide/ eudesmanoid sesquiterpene compound such as 7-hydroxyfrullanolide (1) derived from Sphaeranthus indicus in combination with a Garcinia mangostana extract standardized to cc-mangostin (2) or ⁇ -mangostin (3) and further optionally
- compositions comprising at least one herbal ingredient selected from the extracts or fractions standardized to 5% 7- hydroxyfrullanolide derived from Sphaeranthus indicus in combination with a Garcinia mangostana extract standardized to 10% a-mangostin and suitable excipients as a natural energy enhancer to provide an enhancing physical or mental performance, onset and steady maintenance of energy and mental alertness in a mammal.
- examples of improved physical performance include increased stamina and improved speed, strength, power, endurance, flexibility, agility, balance, focus coordination, reaction time, fatigue recovery and also increase of sex stamina.
- examples of improved mental performance include improved sharpness, attention span, mental alertness, cognitive functions, mood elevation, and recovery or reduction of mental fatigue (e.g., following a high-intensity physical exercise).
- the invention includes the preparation of dietary supplements, food ingredient formulations such as beverages, snacks and energy drinks containing the above said ingredient(s) or its composition(s) for enhancing physical activity, physical fitness, mental alertness, energy levels, stamina levels, circulatory health, blood vessel health or for better mental health in warm blooded animals in need thereof.
- the invention provides at least one herbal ingredient selected from the extract(s), fraction(s), compound(s), extracts or fractions standardized to active compound(s)/7-hydroxyfrullanolide or its analogs derived from Sphaeranthus indicus and compositions as natural enhancers of energy instantly, physical performance in warm blooded animals in need thereof.
- extracts and/or fractions standardized to 7- hydroxyfrullanolide, its analogs or their compositions can be used for enhancing physical performance or energy levels in warm blooded animals.
- compositions comprising biologically active ingredients in combination with one or more components selected from extract(s), fraction(s), active compound(s), phytochemical(s); powder(s) derived from plant(s), animal(s) or microorganisms with proven therapeutic health benefits; pharmaceutically or dietetically acceptable agents, active ingredients, vitamins, amino acids or minerals.
- composition consisting of Sphaeranthus indicus derived herbal ingredient in combination with at least one biologically active ingredient selected from extract of Garcinia mangostana fruit rind, Osmium basilicum, Zingiber officinalis, Tribulus terrestris, vitamins, amino acids, taurine, an extract of Ginkgo biloba, Rhodiola and an extract of guarana, optionally in combination with an excipient, diluent or carrier as a natural energy enhancer to provide an onset and steady maintenance of energy and mental alertness in a warm blooded animal or mammal in need thereof.
- biologically active ingredient selected from extract of Garcinia mangostana fruit rind, Osmium basilicum, Zingiber officinalis, Tribulus terrestris, vitamins, amino acids, taurine, an extract of Ginkgo biloba, Rhodiola and an extract of guarana, optionally in combination with an excipient, diluent or carrier as a natural energy enhancer to provide an
- the invention discloses the compositions wherein the extract of Garcinia mangostana fruit rind is standardized to a-mangostin or ⁇ -mangostin or both and optionally in combination with at least one excipient/diluent as a natural energy enhancer to provide an onset and steady maintenance of energy and mental alertness in a warm blooded animal or mammal in need thereof.
- the biologically active ingredients of the present invention for preparing the composition may further include one or more of the non- limiting components such as vitamins selected from B vitamins, including thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, biotin, cyanocobalamin, choline and/or folic acid, including the reduced forms of folic acid such as but not limited to folinic acid, calcium folinate and methyltetrahydrofolate.
- B-complex vitamins are also water soluble vitamins that aid the breakdown of carbohydrates into glucose to provide energy for the body, the breakdown of fats and proteins to aid the normal functioning of the nervous system, and muscle tone in the stomach and intestinal tract.
- Particular forms of B vitamins in the composition may include d-Calcium pantothenate, niacinamide, pyridoxine hydrochloride, and thiamine mononitrate; or amino acids.
- the Sphaeranthus indicus derived herbal ingredient selected from the extracts or fractions standardized to 7-hydroxyfrullanolide or its analogs or mixtures thereof and/or their composition(s) increases circulation, metabolism efficiency, regulates neurotransmitters and boosts oxygen levels in the brain.
- Benefits of enhanced circulation in the brain include improved short and long term memory, increased reaction time and improved mental clarity.
- the said herbal ingredients or their composition(s) are useful for enhancing energy levels, mental alertness, stamina levels, circulatory health and blood vessel health as well as for providing nutritional support. It can also be used as an energy booster for patients in need thereof, e.g., cancer patients or AIDS patients. Moreover, it is useful for enhancing athletic performance in both humans and animals, e.g., horses.
- the said herbal ingredients and/or their composition(s) can be taken e.g. by performance athletes, those engaged in endurance and multi-discipline sports, aged and sick persons in order to increase mental and physical efficiency and to repress susceptibility to illnesses, such as cold, bacterial and viral infections, in a natural manner.
- the present ingredients and compositions may be formulated as a "fitness drink" that can be taken with breakfast or in the form of a concentrate from which such a drink can be regularly used.
- 7-hydroxyfrullanolide or its analogs obtained naturally, synthesized or semi-synthesized can be useful for enhancing physical or mental performance.
- one or more of the analogs of 7-hydroxyfrullanolide that can be used for preparing compositions comprise of frullanolides; l la,13-dihydro-3a,7a- dihydroxy-4,5-epoxy-6p,7-eudesmanolide; 11 a, 13-dihydro-7a-acetoxy-3P-hydroxy-6p,7- eudesm-4-enolide; 3-keto-P-eudesmol; 1 la,13-dihydro-3a,7a-dihydroxyeudesm-4-en- 6a, 12-olide; 11 a, 13-dihydro-3a,7a-dihydroxyfrullanolide; 11 a, 13-dihydro-7a, 13- dihydroxyfrullanolide; 1 la,13-dihydro-7a-hydroxy-13-methaoxyfrullanolide; 2a, 7a- dihydroxy-4-en-l l,13-dihydroeudesm-6,12-oli
- concentration of Sphaeranthus indicus derived 7-hydroxyfrullanolide or frullanolide/ eudesmanoid sesquiterpene compounds in the present invention individually or together varies in the range of 0.01% to 99.99%.
- composition wherein, the concentration of Garcinia mangostana fruit rind extract, a- mangostin and ⁇ -mangostin individually or jointly varies from 0.01% to 99.99%.
- the herbal ingredient(s) or composition(s) of the present invention are useful to improve aerobic performance and capacity by influencing lactic acid metabolism and reducing fatigue as well as for alleviation of one or more of the biological energy markers including but not limited to ATP levels/production; Nitric Oxide levels, NADH balance, leptin levels, adipocytes derived peptide
- the herbal ingredient(s) can further be combined with one or more biological active ingredients comprising anti-diabetic, anti-hyperlipidemic, anti-obesity, anti-hypertensive, anti-platelet aggregation, anti-infective, anti-atherosclerotic, antiinflammatory, anti-oxidant and bio-enhancing activity.
- one or more biological active ingredients comprising anti-diabetic, anti-hyperlipidemic, anti-obesity, anti-hypertensive, anti-platelet aggregation, anti-infective, anti-atherosclerotic, antiinflammatory, anti-oxidant and bio-enhancing activity.
- the composition may contain an effervescent.
- an effervescent is an agent comprising one or more compounds which, act individually or together, evolve a gas on contact with water.
- the gas evolved is generally oxygen or, most commonly, carbon dioxide.
- Preferred effervescent agents comprise an acid component and a base component that react in the presence of water to generate carbon dioxide gas.
- the acid component can comprise one or more acids and the base component can comprise one or more bases.
- the base component comprises an alkali metal or alkaline earth metal carbonate or bicarbonate and the acid component comprises an aliphatic carboxylic acid.
- Non-limiting examples of suitable bases for use in a base component include carbonate salts (e.g., calcium carbonate), bicarbonate salts (e.g., sodium bicarbonate), sesquicarbonate salts, and mixtures thereof.
- carbonate salts e.g., calcium carbonate
- bicarbonate salts e.g., sodium bicarbonate
- sesquicarbonate salts e.g., sesquicarbonate salts, and mixtures thereof.
- Sodium bicarbonate is a preferred base.
- Non-limiting examples of suitable acids for use in an acid component include citric acid, lactic acid, glutaric acid, phosphoric acid, acetic acid, tartaric acid, malic acid, fumaric acid, adipic acid, succinic acid, oxaloacetate, acid anhydrides of such acids, acid salts of such acids, and mixtures thereof.
- Citric acid is a preferred acid.
- the composition may contain sweeteners.
- Preferred sweeteners for use in the present invention are sugars and sugar alcohols such as sucrose, fructose, dextrose, maltose, lactose, high fructose corn syrup solids, invert sugar, sugar alcohols, including sorbitol, as well as mixtures of these sugars and sugar alcohols. In order to deliver lower levels of solids per dosage, it may be preferred to use a higher intensity sweetener with the sugar or sugar alcohol.
- These higher intensity sweeteners include saccharin, cyclamates, acesulfame K, L-aspartyl-L-phenylalanine lower alkyl ester sweeteners (e.g., aspartame); L-aspartyl-D-alanine amides; L-aspartyl-D-serine amides; L-aspartyl-L-l-hydroxymethylalkaneamide sweeteners; L-aspartyl-1- hydroxyethyalkaneamide sweeteners; and L-aspartyl-D-phenylglycine ester and amide sweeteners.
- L-aspartyl-D-phenylalanine lower alkyl ester sweeteners e.g., aspartame
- L-aspartyl-D-alanine amides e.g., L-aspartyl-D-phenylalanine lower alkyl ester sweeteners
- sweeteners contemplated for use with the compositions disclosed herein include sweeteners derived from stevia, sweeteners derived from momordica grosvenorii, and sweeteners derived from mogrosides.
- a particularly preferred sweetener system is a combination of sucralose with acesulfame K and corn syrup solids.
- the composition may be dissolved in water or in other liquids suitable for human consumption.
- the various suitable solvents that can be used for preparing the extracts and fractions or extracting or fractionating the herbs such as Sphaeranthus indicus, Garcinia mangostana include but not limited to C1-C5 alcohols, like ethanol, methanol; water and mixtures thereof; C1-C7 hydrocarbons such as hexane; esters like ethyl acetate and the like and mixtures thereof.
- the ingredients and compositions of the present invention may be formulated in dry form, liquid form, food product, dietary supplement or any suitable form such as tablet, a capsule or a soft chew.
- the herbal ingredient(s) or composition(s) can be delivered in the form of controlled release tablets, using controlled release polymer-based coatings by the techniques including nanotechnology, microencapsulation, colloidal carrier systems and other drug delivery systems.
- herbal ingredient(s) or their compositions nutritional/dietary supplements can be contemplated/made in the dosage form of healthy foods, or food for specified health uses such as solid food like chocolate or nutritional bars, semisolid food like cream or jam, or gel and also beverage and the like, such as refreshing beverage, lactic acid bacteria beverage, drop, candy, chewing gum, gummy candy, yoghurt, ice cream, pudding, soft adzuki bean jelly, jelly, cookie, tea, soft drink, juice, milk, coffee, cereal, snack bar and the like.
- flavorant(s) that may be included in the composition are not relevant to the inventive concepts disclosed herein, and those skilled in the art are familiar with the wide range of flavorants available. Therefore, any suitable flavorant or combination of flavorants, natural and/or artificial, is within the contemplated scope of the present disclosure.
- the composition may further include food colorants to improve the visual appearance of a drink prepared with the composition.
- the flavoring agent or agents may provide a fruit flavor, cola flavor, vanilla flavor, or a chocolate flavor, by way of example and not by way of limitation.
- Sweeteners natural or synthetic, such as sucrose, sucralose, aspartame, and/or acesulfame potassium, neotame, polydextrose, glycerin, sorbitol, and xylitol, may be used, by way of example and not by way of limitation.
- Coloring agents may be added. Agents such as citric acid, fumaric acid, adipic acid, tartaric acid, and in some instances lactic acid may be added to adjust for tartness. Additional ingredients in the form of analgesics (e.g. aspirin), mild stimulants other than caffeine, which is mentioned above (e.g. green tea), or relaxants (e.g. melatonin), may be added in specialized product applications.
- analgesics e.g. aspirin
- mild stimulants other than caffeine which
- the herbal ingredients and composition of this invention are useful in treating mitochondrial deficiencies in both humans and animals. It also can be used for enhancing or maintaining physical or mental performance, reducing infection in physically stressed athletes or non-athletes from intense physical exercises. Moreover, it can be an activator of Sirtuin.
- the biologically acceptable ingredients or compositions can further be combined with one or more pharmaceutically or dietetically acceptable excipients, carriers and diluents, comprising glucose, fructose, sucrose, maltose, yellow dextrin, white dextrin, aerosil, microcrystalline cellulose, calcium stearate, magnesium stearate, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-alpha-tocopherol, glycerin, propylene glycol, glycerin fatty ester, poly glycerin fatty ester, sucrose fatty ester, sorbitan fatty ester, propylene glycol fatty ester, acacia, carrageenan, casein, gelatin, pectin, agar, vitamin B group, nicotinamide, calcium pantothen
- the invention features a method for enhancing physical or mental performance, steady maintenance of energy, physical endurance and mental alertness in humans and animals, wherein the method comprises administering to a subject in need thereof an effective amount of at least one herbal ingredient selected from the extract(s), fraction(s), compound(s), extracts or fractions standardized to active compound(s)/7-hydroxyfruIlanolide derived from Sphaeranthus indicus and compositions thereof.
- improved physical performance include increased stamina and improved speed, strength, power, endurance, flexibility, agility, balance, focus coordination, reaction time, fatigue recovery and also increase of sex stamina.
- improved mental performance examples include improved sharpness, attention span, mental alertness, cognitive functions, mood elevation, and recovery or reduction of mental fatigue (e.g., following a high-intensity physical exercise).
- the invention features use of at least one herbal ingredient selected from the extracts), fraction(s), compound(s), extracts or fractions standardized to active compound(s)/7-hydroxyfrullanolide derived from Sphaeranthus indicus and compositions thereof to enhance physical activity, physical fitness, mental alertness, enhancing energy levels, stamina levels, circulatory health, blood vessel health and for better mental health in warm blooded animal or humans or animals.
- Sphaeranthus indicus methanol extract (LI12500): Sphaeranthus indicus flower heads (1 kg) were taken in a round bottom flask and extracted with methanol (8 L) at 60 - 65 °C temperature for 2 h. The extract was filtered and the spent raw material was re-extracted twice with methanol (2 x 6 L) under similar conditions. The combined extract was fine filtered and concentrated over a climbing film evaporator to obtain a residue (110 g). The methanol extract (LI 12500) showed 11% of 7-hydroxyfrullanolide by HPLC method of analysis.
- Sphaeranthus indicus methanol extract standardized to 5% 7-hydroxyfrullanolide Sphaeranthus indicus methanol extract containing 7-hydroxyfrullanolide as obtained above was diluted with microcrystalline cellulose and magnesium strearate along with 2% by weight of aerosil to obtain Sphaeranthus indicus powdered methanol extract standardized to 5% 7-hydroxyfrullanolide (LI12508).
- Sphaeranthus indicus ethanol extract Sphaeranthus indicus flower heads (1 kg) were taken in a RB flask and extracted with ethanol (8 L) at 60- 65°C temperature for 2 h. The extract was filtered and the spent raw material was re-extracted twice with ethanol (2 x 6 L) under similar conditions. The combined extract was fine filtered and concentrated over a climbing film evaporator to obtain a residue (98 g). The ethanol extract showed 12% of 7-hydroxyfrullanolide by HPLC method of analysis.
- Garcinia mangostana rind methanol extract (LI80013): Shade dried fruit rind (IKg) of Garcinia mangostana was pulverized to coarse powder, and extracted with 90% methanol (5L) for 2 hrs at 60-65 ° C. The solvent was separated from the raw material by filtration. Extraction process was repeated three times using 90% methanol (2x3 L & 1x2 L). The combined extracts were fine filtered and concentrated under reduced pressure and allowed to precipitate at ambient temperature. The solid separated was filtered to give a dry powder (150 g, a-mangostin: 21% and ⁇ -mangostin: 2.5%).
- Garcinia mangostana methanol extract containing ⁇ -mangostin as obtained above was diluted with microcrystalline cellulose and magnesium strearate along with 2% aerosil to obtain Garcinia mangostana methanol extract standardized to 10% a- mangostin (LI80018).
- Example 5 Garcinia mangostana methanol extract containing ⁇ -mangostin as obtained above (example 3) was diluted with microcrystalline cellulose and magnesium strearate along with 2% aerosil to obtain Garcinia mangostana methanol extract standardized to 10% a- mangostin (LI80018).
- Garcinia mangostana rind ethanol extract Shade dried fruit rind (IKg) of Garcinia mangostana was pulverized to coarse powder, and extracted with 90% ethanol (5L) for 2 hrs at 60-65 ° C. The solvent was separated from the raw material by filtration. Extraction process was repeated thrice using 90% ethanol (2x3 L & 1x2 L). The combined extracts were fine filtered and concentrated under reduced pressure and allowed to precipitate at ambient temperature. The solid separated was filtered to give ethanol extract as a dry powder (136 g, a-Mangostin: 22%).
- composition LI12506F The composition LI12506F was prepared by combining the methanol extract of Sphaeranthus indicus (LI 12500) and 90% methanol extract of Garcinia mangostana (LI80013), both as prepared above, in the ratio of 3: 1.
- Formulation LI12507F (Merastin ® ): The Sphaeranthus indicus powder extract standardized to minimum 5% 7-hydroxyfrullanolide (LI 12508) and Garcinia mangostana methanol extract standardized to 10% a-mangostin (LI80018) as prepared above were combined at 3 : 1 ratio and properly blended to obtain LI12507F.
- Composition LI12517F was prepared by mixing unit doses of the following components: One part of Sphaeranthus indicus ethanol extract (1 g) and
- Sixty obese subjects (27.6 % male; 72.4 % female; ages 22-50 years) were included in a randomized, double-blind, placebo-controlled study of 56 days duration.
- Various parameters including, physical, anthropomorphic, biochemical and questionnaire based evaluation including quality of life have been studied.
- the S. indicus extract (LI 12508) and its formulation LI12507F provided significant reduction in body weight in both the treatment groups and also indicated promise for their potential use in controlling serum triglyceride levels.
- IWQOL-Lite Impact of Weight on Quality of Life Questionnaire-Lite Version (IWQOL-Lite) including subjects feedback was administered to all the subjects at each evaluation point including days 0, 14, 28 and 56 was assessed. Subjects expressed improvement in various parameters including increased mobility, increased walking speed, increased walking distance and time, feeling untired, feeling light and active. Number of subjects expressed such improvement in physical ability and endurance was more in supplement groups compared to placebo group.
- Both LI12508 and LI12507F also showed unexpectedly improved quality of life and improved energy, exercise ability and endurance in subjects supplemented with the treatments when compared the placebo treated group. This includes increased mobility, increased walking speed, increased walking distance, lack of fatigue, feeling untired, feeling light and active.
- Intracellular ATP level in the cells treated cells with LI 12508 and LI12507F were measured by luminescence based ATP detection assay system, ATPlite (PerkinElmer Inc., Waltham, MA). Briefly, equal number of L6 Rat myocytes was plated in each well of a 96-well cell culture plate and cultured in DMEM supplemented with 10% FBS. The cells were treated with different concentrations of test samples (LI12508 and LI12507F) for 4h and thereafter, to each well 50 ul of cell lysis buffer were added. The vehicle control culture wells received 0.2% DMSO (v/v). Finally, 50 ul of substrate solution was added to each well and incubated at room temperature for 5 min on plate shaker.
- mice of 20 weeks old were selected and examined for health status and acclimatized for one week before initiation of the study.
- the animals were randomly divided into three groups each consisting of 6 animals (3 males and 3 females) corresponding to two doses (100 and 250mg kg body weight) of test substance LI12508 and vehicle control.
- the animals were treated orally with allocated test substances or vehicle daily for 1 week.
- swimming exercise (15 min) was initiated after 1 hr of treatment and continued daily for 7 days. Mice were allowed to swim with supporting constant loads (lead fish sinkers, attached to the tail) corresponding to 10% of their body weight, in a acrylic tank filled with water.
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Priority Applications (6)
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CN2011800435489A CN103108556A (en) | 2010-09-11 | 2011-09-07 | Ingredients derived from sphaeranthus indicus |
CA2810793A CA2810793C (en) | 2010-09-11 | 2011-09-07 | Sphaeranthus indicus derived ingredients and their compositions for enhancing physical performance and energy levels |
SG2013016597A SG188424A1 (en) | 2010-09-11 | 2011-09-07 | Ingredients derived from sphaeranthus indicus |
AU2011300376A AU2011300376B2 (en) | 2010-09-11 | 2011-09-07 | Ingredients derived from Sphaeranthus indicus |
KR1020137009108A KR20130119424A (en) | 2010-09-11 | 2011-09-07 | Ingredients derived from sphaeranthus indicus |
US13/793,084 US10105347B2 (en) | 2009-02-02 | 2013-03-11 | Sphaeranthus indicus derived ingredients and their compositions for enhancing physical performance and energy levels |
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PCT/IN2010/000053 Continuation-In-Part WO2010100653A2 (en) | 2009-02-02 | 2010-02-01 | Composition from sphaeranthus indicus and garcinia mangostana for the control of metabolic syndrome |
US13146965 Continuation-In-Part | 2011-07-29 |
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CN (1) | CN103108556A (en) |
AU (1) | AU2011300376B2 (en) |
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KR20150101458A (en) * | 2013-01-03 | 2015-09-03 | 라일라 뉴트라슈티칼스 | Synergistic dietary supplement for enhancing physical performance |
US9872871B2 (en) | 2013-04-23 | 2018-01-23 | Aarhus Universitet | Compositions for use in restoring muscle glycogen and/or muscle mass |
CN109475167A (en) * | 2016-05-21 | 2019-03-15 | 莱拉运动营养有限公司 | For improving the dietary supplements and composition of physical fitness and energy level |
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WO2010100653A2 (en) * | 2009-02-02 | 2010-09-10 | Laila Nutraceuticals | Composition from sphaeranthus indicus and garcinia mangostana for the control of metabolic syndrome |
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US20070196298A1 (en) * | 2005-05-31 | 2007-08-23 | Kostick Richard H | Cosmetic and dermatological formulations with natural pigments and methods of use |
EP1931366B1 (en) * | 2005-09-30 | 2013-05-29 | Piramal Enterprises Limited | Herbal composition for inflammatory disorders |
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- 2011-09-07 MY MYPI2013700389A patent/MY166221A/en unknown
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Cited By (7)
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KR20150101458A (en) * | 2013-01-03 | 2015-09-03 | 라일라 뉴트라슈티칼스 | Synergistic dietary supplement for enhancing physical performance |
CN104902912A (en) * | 2013-01-03 | 2015-09-09 | 莱拉营养食品有限公司 | Synergistic dietary supplement compositions for enhancing physical performance and energy levels |
EP2941261A4 (en) * | 2013-01-03 | 2016-12-07 | Laila Nutraceuticals | Synergistic dietary supplement compositions for enhancing physical performance and energy levels |
EP3590521A1 (en) * | 2013-01-03 | 2020-01-08 | Laila Nutraceuticals | Synergistic dietary supplement compositions for enhancing physical performance and energy levels |
KR102161601B1 (en) * | 2013-01-03 | 2020-10-05 | 라일라 뉴트라슈티칼스 | Synergistic dietary supplement for enhancing physical performance |
US9872871B2 (en) | 2013-04-23 | 2018-01-23 | Aarhus Universitet | Compositions for use in restoring muscle glycogen and/or muscle mass |
CN109475167A (en) * | 2016-05-21 | 2019-03-15 | 莱拉运动营养有限公司 | For improving the dietary supplements and composition of physical fitness and energy level |
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AU2011300376B2 (en) | 2015-12-03 |
WO2012032544A3 (en) | 2012-05-10 |
KR20130119424A (en) | 2013-10-31 |
MY166221A (en) | 2018-06-22 |
CA2810793C (en) | 2023-10-31 |
SG188424A1 (en) | 2013-04-30 |
AU2011300376A1 (en) | 2013-04-04 |
CA2810793A1 (en) | 2012-03-15 |
CN103108556A (en) | 2013-05-15 |
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