WO2012027374A3 - Novel fungal carbohydrate hydrolases - Google Patents

Novel fungal carbohydrate hydrolases Download PDF

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Publication number
WO2012027374A3
WO2012027374A3 PCT/US2011/048817 US2011048817W WO2012027374A3 WO 2012027374 A3 WO2012027374 A3 WO 2012027374A3 US 2011048817 W US2011048817 W US 2011048817W WO 2012027374 A3 WO2012027374 A3 WO 2012027374A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
relates
novel
methods
compositions
Prior art date
Application number
PCT/US2011/048817
Other languages
French (fr)
Other versions
WO2012027374A2 (en
Inventor
Johannes Visser
Sandra Hinz
Jan Werij
Jacob Visser
Vivi Joosten
Martijn Koetsier
Mark Emalfarb
Original Assignee
Dyadic International (Usa) Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dyadic International (Usa) Inc. filed Critical Dyadic International (Usa) Inc.
Publication of WO2012027374A2 publication Critical patent/WO2012027374A2/en
Publication of WO2012027374A3 publication Critical patent/WO2012027374A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00Methods for preparing or baking dough
    • A21D8/02Methods for preparing dough; Treating dough prior to baking
    • A21D8/04Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055Alpha-N-arabinofuranosidase (3.2.1.55)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes to improve digestability of animal feed and in a variety of other processes, including food and beverage processing, baking, washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
PCT/US2011/048817 2010-08-23 2011-08-23 Novel fungal carbohydrate hydrolases WO2012027374A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37609210P 2010-08-23 2010-08-23
US61/376,092 2010-08-23

Publications (2)

Publication Number Publication Date
WO2012027374A2 WO2012027374A2 (en) 2012-03-01
WO2012027374A3 true WO2012027374A3 (en) 2012-08-16

Family

ID=45724021

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/048817 WO2012027374A2 (en) 2010-08-23 2011-08-23 Novel fungal carbohydrate hydrolases

Country Status (1)

Country Link
WO (1) WO2012027374A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7319920B2 (en) 2017-01-04 2023-08-02 ノボザイムス アクティーゼルスカブ Microbial lysozyme for use in treating irritable bowel syndrome or inflammatory bowel disease

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CA2807702C (en) 2010-08-20 2018-07-24 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
US20130288310A1 (en) * 2010-08-23 2013-10-31 Codexis, Inc Recombinant lignocellulose degradation enzymes for the production of soluble sugars from cellulosic biomass
EP2780450A4 (en) * 2011-11-15 2015-09-09 Novozymes Inc Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
AU2012359280B2 (en) 2011-12-19 2016-08-25 Novozymes A/S Processes and compositions for increasing the digestibility of cellulosic materials
BR112014030463A2 (en) * 2012-06-08 2017-06-27 Concordia Univ innovative enzymes for cell wall deconstruction of scytalidium thermophilum, myriococcum thermophilum and aureobasidium pullulans and their uses
WO2013182670A2 (en) * 2012-06-08 2013-12-12 Dsm Ip Assets B.V. Novel cell wall deconstruction enzymes of scytalidium thermophilum and uses thereof
WO2013182669A2 (en) * 2012-06-08 2013-12-12 Dsm Ip Assets B.V. Novel cell wall deconstruction enzymes of myriococcum thermophilum and uses thereof
BR112014031024A2 (en) * 2012-06-11 2017-06-27 Codexis Inc fungal xylanases and xylosidases.
KR101535526B1 (en) * 2012-06-26 2015-07-09 한국생명공학연구원 Fusion proteins of GH61 with enhanced secretory capability comparing to its wild-type
WO2014005499A1 (en) * 2012-07-02 2014-01-09 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP2772545A1 (en) * 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
EP3074513A1 (en) 2013-11-26 2016-10-05 Novozymes A/S Enzyme compositions and uses thereof
EP3152315B1 (en) 2014-06-06 2018-08-15 Novozymes A/S Enzyme compositions and uses thereof
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
BR112017018461A2 (en) 2015-03-12 2018-04-17 Novozymes As multistage hydrolysis processes for producing a fermentation product and for increasing hydrolysis sugar yield.
BR112017019331A2 (en) 2015-03-12 2018-06-05 Beta Renewables Spa multistage saccharification processes of a lignocellulosic material and to improve a glucose or xylose yield of saccharification of a lignocellulosic material in a continuously stirred tank reactor
TN2017000318A1 (en) 2015-03-12 2019-01-16 Beta Renewable Spa Multi-stage enzymatic hydrolysis of lignocellulosic biomass
WO2016205127A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
BR112019012771A2 (en) * 2016-12-21 2019-12-10 Novozymes As gh25 isolated polypeptide, methods for hydrolyzing peptidoglycan in bacterial cell walls, to increase digestibility of peptidoglycans in animal feed walls, to produce polypeptide, to improve intestinal health in an animal and to promote the elimination of dead lactobacillus johnsonii cells, additive animal feed, animal feed, composition, polynucleotide, recombinant host cell, use of the polypeptide, and zootechnical additive for use in feed.
CN110139666A (en) 2016-12-21 2019-08-16 诺维信公司 Polypeptide with lysozyme activity, the polynucleotides for encoding the polypeptide and application thereof and composition
MX2019014889A (en) 2017-06-28 2020-02-13 Novozymes As Polypeptides having trehalase activity and polynucleotides encoding same.
BR112020002115A2 (en) 2017-08-08 2020-08-11 Novozymes A/S trehalase variant polypeptide, polynucleotide, nucleic acid construct, expression vector, host cell, composition, whole broth formulation or cell culture composition, method of producing a trehalase variant, and, production process of a product of fermentation
EP3467171A1 (en) * 2017-10-06 2019-04-10 Lenzing Aktiengesellschaft Lyocell filament denim
CN108004153B (en) * 2017-12-07 2021-05-28 潍坊康地恩生物科技有限公司 Trichoderma reesei strain capable of producing pectin lyase in high yield and application thereof
CN108265092B (en) * 2017-12-26 2021-07-27 天津大学 Mushroom oligosaccharide with excellent antioxidant activity and preparation method thereof
US20210198618A1 (en) 2018-05-31 2021-07-01 Novozymes A/S Processes for enhancing yeast growth and productivity
WO2019228448A1 (en) * 2018-06-01 2019-12-05 Novozymes A/S Polypeptides
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024003143A1 (en) * 2022-06-30 2024-01-04 Novozymes A/S Mutanases and oral care compositions comprising same

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Non-Patent Citations (3)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7319920B2 (en) 2017-01-04 2023-08-02 ノボザイムス アクティーゼルスカブ Microbial lysozyme for use in treating irritable bowel syndrome or inflammatory bowel disease

Also Published As

Publication number Publication date
WO2012027374A2 (en) 2012-03-01

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