WO2012018226A3 - 카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법 - Google Patents

카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법 Download PDF

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WO2012018226A3
WO2012018226A3 PCT/KR2011/005709 KR2011005709W WO2012018226A3 WO 2012018226 A3 WO2012018226 A3 WO 2012018226A3 KR 2011005709 W KR2011005709 W KR 2011005709W WO 2012018226 A3 WO2012018226 A3 WO 2012018226A3
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cadaverine
high production
mutant microorganism
same
preparation
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PCT/KR2011/005709
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French (fr)
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WO2012018226A2 (ko
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이상엽
전지강
하소하
김민영
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한국과학기술원
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Priority to US13/813,949 priority Critical patent/US9115362B2/en
Priority to EP11814826.1A priority patent/EP2602314A4/en
Publication of WO2012018226A2 publication Critical patent/WO2012018226A2/ko
Publication of WO2012018226A3 publication Critical patent/WO2012018226A3/ko

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Abstract

본 발명은 카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법에 관한 것으로, 보다 구체적으로는 카다베린(cadaverine)의 분해 또는 이용 경로에 관여하는 유전자가 약화 또는 결실되어 있는 카다베린 고생성능을 가지는 변이 미생물 및 이를 호기조건하에서 배양하여 퓨트레신을 고수율로 제조하는 방법에 관한 것이다. 본 발명에 따른 카다베린 고생성능을 가지는 변이 미생물은 산업적으로 널리 사용되는 카다베린을 고수율로 제조하는데 유용하다.
PCT/KR2011/005709 2010-08-03 2011-08-03 카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법 WO2012018226A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/813,949 US9115362B2 (en) 2010-08-03 2011-08-03 Mutant microorganism having high production of cadaverine, and preparation method of cadaverine using same
EP11814826.1A EP2602314A4 (en) 2010-08-03 2011-08-03 MUTANT MICROORGANISM HAVING LARGE PRODUCTION OF CADAVERIN, AND PROCESS FOR PREPARING CADAVERIN USING THE SAME

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100075066A KR101231897B1 (ko) 2010-08-03 2010-08-03 카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법
KR10-2010-0075066 2010-08-03

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WO2012018226A2 WO2012018226A2 (ko) 2012-02-09
WO2012018226A3 true WO2012018226A3 (ko) 2012-05-24

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PCT/KR2011/005709 WO2012018226A2 (ko) 2010-08-03 2011-08-03 카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법

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US (1) US9115362B2 (ko)
EP (1) EP2602314A4 (ko)
KR (1) KR101231897B1 (ko)
WO (1) WO2012018226A2 (ko)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015095298A2 (en) * 2013-12-17 2015-06-25 University Of Florida Research Foundation, Incorporated Use of polyamines and polyamine transporters to provide furfural tolerance
KR101539370B1 (ko) * 2014-05-14 2015-07-24 씨제이제일제당 주식회사 L-라이신을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-라이신의 생산방법
KR20160097691A (ko) 2015-02-09 2016-08-18 씨제이제일제당 (주) 신규 라이신 디카르복실라제 및 이를 이용하여 카다베린을 생산하는 방법
KR101791837B1 (ko) * 2015-08-06 2017-10-31 서울대학교산학협력단 라이신 디카르복실라아제의 변이주 개발 방법 및 그의 응용
KR101722984B1 (ko) * 2015-11-26 2017-04-07 건국대학교 산학협력단 바륨-알지네이트 담체를 이용한 라이신 디카르복실라아제 과발현 재조합 대장균의 고정화를 통한 고농도 카다베린 생산방법
KR101871037B1 (ko) * 2016-03-15 2018-06-26 씨제이제일제당 (주) 퓨트레신을 생산하는 미생물 및 이를 이용한 퓨트레신 생산 방법
CN110291101B (zh) * 2016-12-30 2023-08-08 上海凯赛生物技术股份有限公司 修饰的赖氨酸脱羧酶
USD877212S1 (en) 2018-05-15 2020-03-03 Samsung Electronics Co., Ltd. Refrigerator
USD874527S1 (en) 2018-06-19 2020-02-04 Samsung Electronics Co., Ltd. Refrigerator
USD883347S1 (en) 2018-06-19 2020-05-05 Samsung Electronics Co., Ltd. Refrigerator
KR102281525B1 (ko) 2019-10-17 2021-07-26 경희대학교 산학협력단 카다베린 고생성능을 가지는 형질전환 메탄자화균 및 이의 용도
KR20240000147A (ko) * 2022-06-23 2024-01-02 서울대학교산학협력단 용해도 증대 라이신 디카르복실라아제 변이주 개발 및 그의 응용

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090005099A (ko) * 2006-03-30 2009-01-12 바스프 에스이 카다베린의 제조 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2088826B1 (es) 1995-02-10 1997-06-01 Taguidell Sociedad Limitada Procedimiento de obtencion de las diaminas putrescina y cadaverina a partir de productos naturales tratados, su uso como aditivo en abonos, y abono correspondiente.
KR101188432B1 (ko) * 2008-04-10 2012-10-08 한국과학기술원 퓨트레신 고생성능을 가지는 변이 미생물 및 이를 이용한 퓨트레신의 제조방법
EP2513302A1 (en) * 2009-12-17 2012-10-24 Basf Se Processes and recombinant microorganisms for the production of cadaverine

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090005099A (ko) * 2006-03-30 2009-01-12 바스프 에스이 카다베린의 제조 방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KURIHARA, S. ET AL.: "y-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12.", J. BIOL. CHEM., vol. 283, no. 29, 2008, pages 19981 - 19990, XP055070572 *
QIAN, Z.-G. ET AL.: "Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine.", BIOTECHNOL. BIOENG., vol. 108, no. 1, 30 September 2010 (2010-09-30), pages 93 - 103, XP055070575 *
QIAN, Z.-G. ET AL.: "Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine.", BIOTECHNOL. BIOENG., vol. 104, no. 4, 2009, pages 651 - 662, XP055032553 *

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US20130157323A1 (en) 2013-06-20
EP2602314A4 (en) 2014-03-12
KR101231897B1 (ko) 2013-02-08
EP2602314A2 (en) 2013-06-12
WO2012018226A2 (ko) 2012-02-09
KR20120021491A (ko) 2012-03-09
US9115362B2 (en) 2015-08-25

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