WO2011119962A3 - Microfluidic enrichment of selected cell populations - Google Patents

Microfluidic enrichment of selected cell populations Download PDF

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Publication number
WO2011119962A3
WO2011119962A3 PCT/US2011/029999 US2011029999W WO2011119962A3 WO 2011119962 A3 WO2011119962 A3 WO 2011119962A3 US 2011029999 W US2011029999 W US 2011029999W WO 2011119962 A3 WO2011119962 A3 WO 2011119962A3
Authority
WO
WIPO (PCT)
Prior art keywords
blood cells
white blood
whole blood
cell populations
selected cell
Prior art date
Application number
PCT/US2011/029999
Other languages
French (fr)
Other versions
WO2011119962A2 (en
Inventor
Mehmet Toner
Alan Fishman
Ronald G. Tompkins
Ravi Kapur
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Publication of WO2011119962A2 publication Critical patent/WO2011119962A2/en
Publication of WO2011119962A3 publication Critical patent/WO2011119962A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Ecology (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods, devices, and systems for microfluidic enrichment of selected cell populations are described. Methods of labeling white blood cells include obtaining whole blood drawn from a patient, separating the white blood cells from the whole blood by flowing the whole blood through a microfluidic device that includes one or more arrays of obstacles configured to deflect the white blood cells in a direction away from a direction of remaining components of the whole blood, and collecting the separated white blood cells. A buffer containing a labeling agent with an affinity for white blood cells is mixed with the white blood cells either before or after separating the white blood cells from the whole blood resulting in labeled white blood cells.
PCT/US2011/029999 2010-03-26 2011-03-25 Microfluidic enrichment of selected cell populations WO2011119962A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31806510P 2010-03-26 2010-03-26
US61/318,065 2010-03-26

Publications (2)

Publication Number Publication Date
WO2011119962A2 WO2011119962A2 (en) 2011-09-29
WO2011119962A3 true WO2011119962A3 (en) 2012-01-12

Family

ID=44673890

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/029999 WO2011119962A2 (en) 2010-03-26 2011-03-25 Microfluidic enrichment of selected cell populations

Country Status (1)

Country Link
WO (1) WO2011119962A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105247042A (en) * 2013-03-15 2016-01-13 普林斯顿大学理事会 Methods and devices for high throughpout purification

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11493428B2 (en) 2013-03-15 2022-11-08 Gpb Scientific, Inc. On-chip microfluidic processing of particles
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
JP6148101B2 (en) * 2013-07-26 2017-06-14 京セラ株式会社 Cutting tools
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
US10844353B2 (en) 2017-09-01 2020-11-24 Gpb Scientific, Inc. Methods for preparing therapeutically active cells using microfluidics

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019300A1 (en) * 2002-07-26 2004-01-29 Leonard Leslie Anne Microfluidic blood sample separations
US20070059718A1 (en) * 2005-09-15 2007-03-15 Mehmet Toner Systems and methods for enrichment of analytes
US20070160503A1 (en) * 2003-06-13 2007-07-12 Palaniappan Sethu Microfluidic systems for size based removal of red blood cells and platelets from blood
US20080023399A1 (en) * 2006-06-01 2008-01-31 Inglis David W Apparatus and method for continuous particle separation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019300A1 (en) * 2002-07-26 2004-01-29 Leonard Leslie Anne Microfluidic blood sample separations
US20070160503A1 (en) * 2003-06-13 2007-07-12 Palaniappan Sethu Microfluidic systems for size based removal of red blood cells and platelets from blood
US20070059718A1 (en) * 2005-09-15 2007-03-15 Mehmet Toner Systems and methods for enrichment of analytes
US20080023399A1 (en) * 2006-06-01 2008-01-31 Inglis David W Apparatus and method for continuous particle separation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105247042A (en) * 2013-03-15 2016-01-13 普林斯顿大学理事会 Methods and devices for high throughpout purification
CN105247042B (en) * 2013-03-15 2021-06-11 普林斯顿大学理事会 Method and apparatus for high throughput purification

Also Published As

Publication number Publication date
WO2011119962A2 (en) 2011-09-29

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