WO2011099022A1 - Composition and method of preparation of fungal based bio insecticide from combination of metarhizium anisopliae, beauveria bassiana and verticillium lecanii fungus with enzymes, fats and growth promoting molecules for controlling various foliage pest and soil borne insect - Google Patents
Composition and method of preparation of fungal based bio insecticide from combination of metarhizium anisopliae, beauveria bassiana and verticillium lecanii fungus with enzymes, fats and growth promoting molecules for controlling various foliage pest and soil borne insect Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- the present invention relates generally to a composition and method of preparation of fungal based bio insecticide from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules for controlling various foliage pest and soil borne insect.
- the invention relates to a novel fungus of Metarhizium genus and a method for controlling soil pests using the same, more particularly to a novel Metarhizium anisopliae HY-2 and a microbial insecticide comprising the same and a method for controlling soil pests using the same.
- the Metarhizium anisopliae HY-2 can effectively be used to control the soil pests such as Scarabaeidae.
- inventions provides an enzyme preparation comprising at least one protease derived from an entomopathogenic fungus of one of the following genera: Metarhizium, Beauvaria, Verticillium, and Aschersonia, and characterised by the following properties: (a) substantially no activity against hide protein azure, locust cuticle and elastin; (b) specificity for Bz-Phe-Val-Arg-NA; (C) not inhibited by soybean trypsin inhibitor.
- an enzyme preparation comprising at least one protease derived from an entomopathogenic fungus of one of the following genera: Metarhizium, Beauvaria, Verticillium, and Aschersonia, and characterised by the following properties: (a) substantially no activity against hide protein azure, locust cuticle and elastin; (b) specificity for Bz-Phe-Val-Arg-NA; (C) not inhibited by soybean trypsin inhibitor.
- the invention relates to a novel fungus of Metarhizium genus and a method for controlling soil pests using the same, more particularly to a novel Metarhizium anisopliae HY-2 and a microbial insecticide comprising the same and a method for controlling soil pests using the same.
- the Metarhizium anisopliae HY-2 can effectively be used to control the soil pests such as Scarabaeidae.
- invention relates to method for selecting fungal species of strains metarhizium anisopliae suitable for control of a pest insect, species (termites) comprising applying conidia of a test fungal species of a strain to the cuticle of at least one target insect, introducing said at least one conidia-carrying target insect to a known number of uninfected target insects, and determining the proportion of dead target insects after a predetermined period.
- PCT/US2002/020030 - Patent Application wherein the invention provides a methods and compositions for control of powdery mildew comprising a novel Verticillium lecanii strain that exhibits antifungal activity both greenhouses and outdoors.
- the invention also provides use of a cell-free extract produced by Verticillium lecanii for control of powdery mildew in plants, and particularly in strawberries and grapes.
- the invention relates to a method for controlling harmful organisms in genetically modified cotton plants which contain a gene derived from Bacillus thuringiensis which encodes and expresses an insecticidally active protein, which comprises applying abamectin to the plants, to their seeds or propagation stock and/or to the area in which they are cultivated at an application rate of from 0.001 to 0.3 kg/ha.
- the known insecticides or pesticides have adopted different process for controlling foliage pest, soil pest or soil borne insects.
- the present invention is based upon a fungal based bio insecticide having innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules used for controlling pests like aphids, : whitefly, thrips, mite, jassids, Mealybug, and caterpillars, as well as soil borne insects like white grub, termite and alike.
- the inventor has invented a composition and method of making a fungal based bio insecticide from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules
- Pests which can be clearly seen on the leaves and other parts of the plant include greenfly and blackfly (aphids) and whitefly. Scale insects and mealy bugs can also be spotted quite readily.
- aphids Various species of aphids will attack most garden plants and can be seen massed on stems and flowers as well as leaves.
- Aphids are sap feeders and can take in large amounts of plant sap. Excess sugars are excreted in the form of a sugary substance, known as honeydew. In some cases, ants will 'farm' aphids for this, which gives the pests some protection from natural predators. Aphid attack can be very debilitating, causing weakening and distortion of the new growth, in particular.
- a secondary symptom of aphid attack is the growth of black sooty moulds, encouraged by the presence of honeydew.
- Whiteflies are also sap feeders. They look like tiny moths and attack many greenhouse and garden plants, especially fuchsias, pelargonium, cucumbers and brassicas.
- Scale insects are brown, almost stationary 'scale-like' creatures which affect a wide range of plants.
- Mealy bugs have a white covering, which often appears as a gooey, cotton-wool like mass in the leaf axils. Both scale insects and mealy bugs are sap feeders and, like aphids, they secrete honeydew and infestation can lead to the presence of sooty mould.
- Pests in this category include Slugs and snails, caterpillars, capsid bugs, flea beetles and weevils such as the pea and bean weevil and adult vine weevil.
- Slugs and snails are a particular problem in mild and/or damp weather. They eat the leaves of most plants, with a rasping action, leaving irregular shaped holes or tattered shredded edges. Young plants can be completely eaten and slime trails can often be seen on the soil surface around affected plants. Slug pellets are the most common means of slug control. Sometimes gardeners are concerned about using pellets if they have pets. There is no need for concern if they are used sparingly and according to label directions. Some customers may prefer to use a liquid preparation which is less accessible to pets than pellets. Caterpillars can attack most plants, their biting action causing relatively large, irregular but clean cut holes in the leaves.
- Caterpillar frass may be seen on the leaves, which serves as a further diagnostic feature of attack.
- Flea beetles cause damage on turnips, radishes, wallflowers and brassicas, especially at the seedling stage, where small, almost circular holes can be seen on affected leaves.
- this invention provides the composition and method of preparing fungal based product from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules. It is used for all sucking pests like aphids, whitefly, thrips, mite, jassids, Mealybug, and caterpillars and as well as soil borne insects like white grub, termite and alike
- Step-2 Starter culture (In flask)
- Culture grown on plate is inoculated aseptically in a flask (1000 ml capacity) having 500 ml PAC-00 broth media via cork borer (having 5 mm diameter cutting capacity), and allow to grow at 26 ⁇ 1 °C for at least 3 to 4 days.
- flask is put on shaker with agitation of media at 150 RPM. Such two flasks are maintained.
- Composition for one liter of PAC-00 broth Media is 300 gm potato, 20 gm dextrose and 1000 ml distilled water
- Step-3 Seed Fermentor (In small fermentor)
- One (1) lit culture grown in step-2 is inoculated aseptically in small fermenter having 10 litter PAC-00 media (Composition for one liter of PAC-00 broth Media is 300 gm potato, 20 gm dextrose and 1000 ml distilled water), and allow for further growth at 26 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM
- Step-4 Add to solid substrate (poly bags)
- Step-8 Mixing (Liquid carrier, emulsifier)
- 10 % fungal spore powder is than mixing in mineral oil (ground nut oil) and tween- 80 emulsifier mixture (90 % ground nut oil + 10 % tween-80) or in case of submerge technique homogenize mixture having spore of fungus where again homogenized by adding 10 to 15 % tween-80 which act as a emulsifier.
- Mix formulation is allowed to cure for two to three days in drums and than send for storage or for packing
- Step-11 Distrubution to farmer,retailer,distributor and others.
- the final material prepared has to be used in following form: Dose in one Acre: Liquid Form: 50 ml to 100 ml
Abstract
This invention is based upon composition and method of preparing an provides the composition and method of preparing fungal based product from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and VerticilHum lecanii fungus with enzymes, fats and growth promoting molecules. It is used for controlling pests like aphids, whitefly, thrips, mite, jassids, Mealybug, and caterpillars and as well as soil borne insects like white grub, termite and alike.
Description
Composition and Method of Preparation of Fungal Based Bio Insecticide From Combination of Metarhizium Anisopliae, Beauveria Bassiana And Verticillium Lecanii Fungus with Enzymes, Fats and Growth Promoting Molecules for Controlling Various Foliage Pest and Soil Borne Insect.
PREAMBLE OF INVENTION- This invention is particular described the nature of the invention and the manner in which it is to be performed.
FIELD OF INVENTION- The present invention relates generally to a composition and method of preparation of fungal based bio insecticide from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules for controlling various foliage pest and soil borne insect.
PRIOR ART-
In the existing system as given in United States Patent Application No. 7416880 - Patent Application, wherein the invention relates to a novel fungus of Metarhizium genus and a method for controlling soil pests using the same, more particularly to a novel Metarhizium anisopliae HY-2 and a microbial insecticide comprising the same and a method for controlling soil pests using the same. The Metarhizium anisopliae HY-2 can effectively be used to control the soil pests such as Scarabaeidae.
In the existing system as given in WIPO Patent Application No. PCT/CN2006/002942 - Patent Application wherein invention discloses an isolated Metarhizium anisopliae variant (M. anisopliae var. dcjhyium), which is referred as Metarhizium anisopliae LjOl .
It is identified as a new variant of Metarhizium anisopliae by the morphologic, physiologic, biochemical and molecular biologic analysis (GCTCC No. M206077, GenBank accession No. DQ288247). Also disclosed are the bioinsecticide produced by said variant and the use of the variant in the extermination of pests, such as termites.
In the existing system as given in United States Patent Application No. 6939688 - Granted Patent wherein the invention relates to the biological addition to organic- mineral fertilizers contains two bacteria associations taken in the ratio 1-2:0.5-1, with one of the associations containing Azotobacter chroococcum and Beijerinckia fluminensis nitrogen binding bacteria in the ratio 1-0.5: 1-0.5 and the other association containing Bacillus megaterium and Bacillus mucilaginosis bacteria, which decompose phosphorus- and potassium containing compounds, in the ratio 1-5:0.5-2, as well as a preservative and micro- and macroelements
In the existing system as given in European Patent Application number EP0335023- Granted Patent wherein invention provides, provides an enzyme preparation comprising at least one protease derived from an entomopathogenic fungus of one of the following genera: Metarhizium, Beauvaria, Verticillium, and Aschersonia, and characterised by the following properties: (a) substantially no activity against hide protein azure, locust cuticle and elastin; (b) specificity for Bz-Phe-Val-Arg-NA; (C) not inhibited by soybean trypsin inhibitor.
In the existing system as given in WIPO patent Application number PCT/KR2002/002039- Granted Patent wherein the invention relates to a novel fungus of Metarhizium genus and a method for controlling soil pests using the same, more particularly to a novel Metarhizium anisopliae HY-2 and a microbial insecticide comprising the same and a method for controlling soil pests using the same. The Metarhizium anisopliae HY-2 can effectively be used to control the soil pests such as Scarabaeidae.
In the existing system as given in CHINA PATENT Number 200310110901 - Granted Patent wherein the invention is one new strain of locust killing fungus of green muscardine, named Metarhizium anisopliae CQMA102 and with conservation number of CGMCC No. 0877. Compared with other locust killing pesticide, the biological pesticide produced with the strain has high toxicity on locust and similar pests, no harm to no-target life forms, heat resistance, powerful contamination tolerance, high spore yield and other excellent characteristics.
In the existing system as given in United States Patent Application No. 5595746 - Granted Patent wherein invention relates to method for selecting fungal species of strains metarhizium anisopliae suitable for control of a pest insect, species (termites) comprising applying conidia of a test fungal species of a strain to the cuticle of at least one target insect, introducing said at least one conidia-carrying target insect to a known number of uninfected target insects, and determining the proportion of dead target insects after a predetermined period.
In the existing system as given in United States Patent Application No. 2009004723- Patent Application wherein the utilizes extracts of the pre-sporulation (preconidial) mycelial stage of entomopathogeiiic fungi as insect attractants and/or pathogens. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material. More than one fungus and substrate can be used in combination.
In the existing system as given in Indian Patent Application No. 163/MAS/2002 - Granted Patent where in the invention relates to relates to a method for preparation of a bioformulation comprising selecting the effective bio-control agents from Paecilomyces lalacinus Bainier, Verticillium chlamydosporium Goddard, Trichoderma harzianum Rifai (TH-1) and TH-2, T. viride Pers. Ex. S. F. Grey, Laetisaria arvalis Burdsall, Gliocladium virens Miller, Giddens & Foster and Bacillus subtilis, preparing a medium by mixing thoroughly sourghum flour, river sand and distilled water, sterilizing the medium in an autoclave, shaking the medium to avoid clogging of the substrates, cooling the medium, inoculating the medium with said effective biocontrol agents in a flask, incubating said flask for few days, collecting the biomass of biocontrol agent alon.gwith media, mixing the said biomass of biocontrol agent and media with a carrier, drying blending the said mixtures air drying the contents and packing them in polythene bags.
In the existing system as given in WIPO Patent Application No. PCT/US2002/020030 - Patent Application wherein the invention provides a methods and compositions for control of powdery mildew comprising a novel Verticillium lecanii strain that exhibits antifungal activity both greenhouses and outdoors. The invention also provides use of a cell-free extract produced by Verticillium lecanii for control of powdery mildew in plants, and particularly in strawberries and grapes.
In the existing system as given in Indian Patent Application No. IN/PCT/2000/772/CHE - Granted Patent wherein the invention relates to a method for controlling harmful organisms in genetically modified cotton plants which contain a gene derived from Bacillus thuringiensis which encodes and expresses an insecticidally active protein, which comprises applying abamectin to the plants, to their seeds or propagation stock and/or to the area in which they are cultivated at an application rate of from 0.001 to 0.3 kg/ha.
OBJECT OF THE INVENTION Traditionally, the known insecticides or pesticides have adopted different process for controlling foliage pest, soil pest or soil borne insects. Whereas the present invention is based upon a fungal based bio insecticide having innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules used for controlling pests like aphids, : whitefly, thrips, mite, jassids, Mealybug, and caterpillars, as well as soil borne insects like white grub, termite and alike.
STATEMENT OF INVENTION- The inventor has invented a composition and method of making a fungal based bio insecticide from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules
DETAILED DESCRIPTION OF INVENTION-
Insects and other pests can badly damage plant roots, stems, leaves, flowers and seeds. Many insect pests multiply very quickly, so it is sensible to check plants regularly, diagnose problems accurately and give the appropriate treatment promptly to prevent a rapid build-up. Eaten, distorted or discolored parts of plants are possible signs of insect damage. In some cases the pest will be clearly visible, but this does not always occur. Descriptions of some of the types of damage caused to various plant parts are given below.
Pests which can be clearly seen on the leaves and other parts of the plant include greenfly and blackfly (aphids) and whitefly. Scale insects and mealy bugs can also be spotted quite readily. Various species of aphids will attack most garden plants and can be seen massed on stems and flowers as well as leaves. Aphids are sap feeders and can take in large amounts of plant sap. Excess sugars are excreted in the form of a sugary substance, known as honeydew. In some cases, ants will 'farm' aphids for this, which gives the pests some protection from natural predators. Aphid attack can be very debilitating, causing weakening and distortion of the new growth, in particular. A secondary symptom of aphid attack is the growth of black sooty moulds, encouraged by the presence of honeydew. Whiteflies are also sap feeders. They look like tiny moths and attack many greenhouse and garden plants, especially fuchsias, pelargonium, cucumbers
and brassicas. Scale insects are brown, almost stationary 'scale-like' creatures which affect a wide range of plants. Mealy bugs have a white covering, which often appears as a gooey, cotton-wool like mass in the leaf axils. Both scale insects and mealy bugs are sap feeders and, like aphids, they secrete honeydew and infestation can lead to the presence of sooty mould.
Some pests will either eat holes in the leaves or eat part or all of the leaf. Pests in this category include Slugs and snails, caterpillars, capsid bugs, flea beetles and weevils such as the pea and bean weevil and adult vine weevil.
Slugs and snails are a particular problem in mild and/or damp weather. They eat the leaves of most plants, with a rasping action, leaving irregular shaped holes or tattered shredded edges. Young plants can be completely eaten and slime trails can often be seen on the soil surface around affected plants. Slug pellets are the most common means of slug control. Sometimes gardeners are concerned about using pellets if they have pets. There is no need for concern if they are used sparingly and according to label directions. Some customers may prefer to use a liquid preparation which is less accessible to pets than pellets. Caterpillars can attack most plants, their biting action causing relatively large, irregular but clean cut holes in the leaves. Caterpillar frass (droppings) may be seen on the leaves, which serves as a further diagnostic feature of attack. Flea beetles cause damage on turnips, radishes, wallflowers and brassicas, especially at the seedling stage, where small, almost circular holes can be seen on affected leaves.
Accordingly, in one aspect, this invention provides the composition and method of preparing fungal based product from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii
fungus with enzymes, fats and growth promoting molecules. It is used for all sucking pests like aphids, whitefly, thrips, mite, jassids, Mealybug, and caterpillars and as well as soil borne insects like white grub, termite and alike
Material and Method used in preparation of above fungal based product is as under Step-1 Nucleus culture (Maintain culture)
Pure culture of fungus (Metarhizium anisopliae, Verticillium lecqnii, Beauveria bassind) is inoculated aseptically on plate having 20 ml PAC-00 agar Media; such two to three plates are generally inoculated. Such inoculated plate is maintained in BOD incubator for at 26 ± 1 °C for 6 to 7 days. (Composition for one liter of PAC-00 Media is 300 gm potato, 20 gm dextrose, 20 gm agar agar and 1000 ml distilled water)
Step-2 Starter culture (In flask)
Culture grown on plate is inoculated aseptically in a flask (1000 ml capacity) having 500 ml PAC-00 broth media via cork borer (having 5 mm diameter cutting capacity), and allow to grow at 26 ± 1 °C for at least 3 to 4 days. For superior growth, flask is put on shaker with agitation of media at 150 RPM. Such two flasks are maintained. (Composition for one liter of PAC-00 broth Media is 300 gm potato, 20 gm dextrose and 1000 ml distilled water)
Step-3 Seed Fermentor (In small fermentor)
One (1) lit culture grown in step-2 is inoculated aseptically in small fermenter having 10 litter PAC-00 media (Composition for one liter of PAC-00 broth Media is 300 gm
potato, 20 gm dextrose and 1000 ml distilled water), and allow for further growth at 26 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM
Step-4 Add to solid substrate (poly bags)
20 ml culture from fermenter is inoculated aseptically in previously sterilized poly propelin polybag (12 x 10 inch) having 180 gm broken rice gain. After inoculation of culture mouth opening of poly bag seal with sponge and allow to develop. Such 500 bag get inoculated from 10 lit broth recovered from fermenter
Step-5 Allow to develop
After inoculation each and every bag, they are transferred to growth room and allowed to develop at 26 ± 1 °C for 9-10 days. During growth (four days after growth) all bag are crushed smoothly by hand to break the clump of mycelium develops on grains. After 10 days of growth all content of bags are removed in trays and transfer to cold room where they maintain at 5 °C till the grinding of grain or submerging of grain having fungal growth in Ground nut oil.
Step-6 Grinding
After sufficient cooling and drying (in cold room at 5 °C) for 10 to 15 days all the grain with the growth of microbes are grinded in grinder or they were brought to submerge technique whereby all the grain were directly submerge in definite quantity of Ground nut oil and then oil-grain mixture is agitate by using commercial in tank agitator.
Step-7 Sieving
Grinding material sieve from 75 micron net with the help of vibro shifter and powder is then collected by using cyclone separator, especially designed for collecting of spore of microbes. And in case of submerge technique oil-grain mixture; sieve from 300 micron sieve and collected material which have fungal spore and mycelium in oil brought to homogenizer.
Step-8 Mixing (Liquid carrier, emulsifier)
10 % fungal spore powder is than mixing in mineral oil (ground nut oil) and tween- 80 emulsifier mixture (90 % ground nut oil + 10 % tween-80) or in case of submerge technique homogenize mixture having spore of fungus where again homogenized by adding 10 to 15 % tween-80 which act as a emulsifier.
Step-9 Curing
Mix formulation is allowed to cure for two to three days in drums and than send for storage or for packing
Step-10 Packging
From above prepared mixture, 50 ml, 100 ml pouch is prepared by using pouch packing machine
Step-11 Distrubution to farmer,retailer,distributor and others.
The final material prepared has to be used in following form: Dose in one Acre: Liquid Form: 50 ml to 100 ml
Claims
1. A composition and method of preparing an improved fungal based product from innovative combination of dormant spore of naturally occurring Metarhizium anisopliae, Beauveria bassiana and Verticillium lecanii fungus with enzymes, fats and growth promoting molecules for controlling pests like aphids, whitefly, thrips, mite, jassids, Mealybug, and caterpillars and as well as soil borne insects like white grub, termite and alike.
2. A composition and method of preparing improved fungal passed product according to claim 1, comprising of inoculating pure culture of fungus Metarhizium anisopliae, Verticillium lecanii and Beauveria bassina aseptically on two or three plates having 20 ml PAC-00 agar Media and maintaining in BOD incubator for at 26 ± 1 °C for 6 to 7 days, wherein composition for one liter of PAC-00 Media contains 300 gm potato, 20 gm dextrose, 20 gm agar agar and 1000 ml distilled water.
3. A composition and method of preparing improved fungal passed product according to claim 1 , wherein culture grown on plate as per method cited in claim 2, is inoculated aseptically in two flask of 1000 ml capacity having 500 ml PAC- 00 broth media via cork borer having 5 mm diameter cutting capacity, and allowing to grow at 26 ± 1 °C for at least 3 to 4 days, whereas for superior growth, flask is put on shaker with agitation of media at 150 RPM, wherein composition for one liter of PAC-00 broth Media contains 300 gm potato, 20 gm dextrose and 1000 ml distilled water.
4. A composition and method of preparing improved fiingal passed product according to claim 1, wherein, One (1) lit culture grown' as per method cited in claim 3 is inoculated aseptically in small fermenter having 10 litter PAC-00 media, wherein composition for one liter of PAC-00 broth Media contains 300 gm potato, 20 gm dextrose and 1000 ml distilled water), and allow for further growth at 26 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM.
5. A composition and method of preparing improved fungal passed product according to claim 1, wherein 20 ml culture from fermenter is inoculated aseptically in previously sterilized poly propelin polybag (12 x 10 inch) having 180 gm broken rice gain and after inoculation of culture mouth opening of poly bag seal with sponge and allowed to develop, wherein 500 bag get inoculated from 10 lit broth recovered from fermenter.
6. A composition and method of preparing improved fungal passed product according to claim 1, wherein, after inoculating each and every bag, the same is transfer to growth room and allowed to develop at 26 ± 1 °C for 9-10 days and during growth at about four days after growth, all bag are crushed smoothly by hand to break the clump of mycelium develops on grains and after 10 days of growth all content of bags are removed in trays and transferred to cold room where they are maintained at 5 °C till the grinding of grin or submerging of grain having fungal growth in Ground nut oil.
7. A composition and method of preparing improved fungal passed product according to claim 1, wherein, after sufficient cooling and drying in cold room at 5 °C for 10 to 15 days, all the grain with the growth of microbes are grinded in grinder or they were brought to submerge technique whereby all the grain were directly submerge in definite quantity of Ground nut oil and then oil-grain mixture is agitate by using commercial in tank agitator.
8. A composition and method of preparing fungal based product according to claim 1, wherein, grinding material sieve from 75 micron net with the help of vibro shifter and powder is collected by using cyclone separator, especially design for collecting of spore of microbes. And in case of submerge technique oil-grain mixture sieve from 300 micron sieve and collected material which have fungal spore and mycelium in oil brought to homogenizer.
9. A composition and method of preparing fungal based product according to claim 1 , wherein, 10 % fungal spore powder is than mixing in mineral oil (ground nut oil) and tween- 80 emulsifier mixture (90 % ground nut oil + 10 % tween-80). or in case of submerge technique homogenize mixture having spore of fungus where again homogenized by adding 10 to 15 % tween-80 which act as a emulsifier.
10. A composition and method of preparing improved fungal passed product according to claim 1, wherein, mixed formulation derived as per claim 8, is allowed to cure for two to three days in drums and then send for storage or for packing in pouch as final output_effective against controlling pest and soil borne insects.
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| IN337/MUM/2010 | 2010-02-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2013096383A3 (en) * | 2011-12-19 | 2013-10-10 | Novozymes Biologicals Holdings A/S | Bio-pestcide methods and compositions |
| WO2014116989A1 (en) | 2013-01-25 | 2014-07-31 | Pioneer Hi-Bred International, Inc. | Maize event dp-032218-9 and methods for detection thereof |
| RU2539025C1 (en) * | 2013-07-08 | 2015-01-10 | Общество с ограниченной ответственностью Малое инновационное предприятие "Кубанские агротехнологии" | Agent for microbiological protection of plants and method of microbiological protection of plants using this agent |
| WO2015069708A1 (en) | 2013-11-08 | 2015-05-14 | Novozymes Bioag A/S | Compositions and methods for treating pests |
| WO2021105846A1 (en) * | 2019-11-28 | 2021-06-03 | Roldan Montoya Jorge Enrique | Biocontrol composition |
| CN115161214A (en) * | 2021-12-30 | 2022-10-11 | 云南云叶化肥股份有限公司 | Biological organic fertilizer for preventing and controlling soil insects, preparation method and application |
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| CN104105403A (en) * | 2011-12-19 | 2014-10-15 | 诺维信生物农业公司 | Bio-pestcide methods and compositions |
| EP3172966A1 (en) * | 2011-12-19 | 2017-05-31 | Novozymes Bioag A/S | Biopesticide method and compositions |
| CN104105403B (en) * | 2011-12-19 | 2017-11-14 | 诺维信生物农业公司 | Biological insecticides method and composition |
| WO2014116989A1 (en) | 2013-01-25 | 2014-07-31 | Pioneer Hi-Bred International, Inc. | Maize event dp-032218-9 and methods for detection thereof |
| RU2539025C1 (en) * | 2013-07-08 | 2015-01-10 | Общество с ограниченной ответственностью Малое инновационное предприятие "Кубанские агротехнологии" | Agent for microbiological protection of plants and method of microbiological protection of plants using this agent |
| WO2015069708A1 (en) | 2013-11-08 | 2015-05-14 | Novozymes Bioag A/S | Compositions and methods for treating pests |
| US10383339B2 (en) | 2013-11-08 | 2019-08-20 | Novozymes Bioag A/S | Compositions and methods for treating pests |
| WO2021105846A1 (en) * | 2019-11-28 | 2021-06-03 | Roldan Montoya Jorge Enrique | Biocontrol composition |
| CN115161214A (en) * | 2021-12-30 | 2022-10-11 | 云南云叶化肥股份有限公司 | Biological organic fertilizer for preventing and controlling soil insects, preparation method and application |
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