WO2011065661A3 - Chondrogenesis of mesenchymal stem cells using dkk-1 or sfrp-1 - Google Patents

Chondrogenesis of mesenchymal stem cells using dkk-1 or sfrp-1 Download PDF

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Publication number
WO2011065661A3
WO2011065661A3 PCT/KR2010/006837 KR2010006837W WO2011065661A3 WO 2011065661 A3 WO2011065661 A3 WO 2011065661A3 KR 2010006837 W KR2010006837 W KR 2010006837W WO 2011065661 A3 WO2011065661 A3 WO 2011065661A3
Authority
WO
WIPO (PCT)
Prior art keywords
mesenchymal stem
stem cells
cells
sfrp
dkk
Prior art date
Application number
PCT/KR2010/006837
Other languages
French (fr)
Korean (ko)
Other versions
WO2011065661A2 (en
Inventor
임군일
Original Assignee
동국대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 동국대학교 산학협력단 filed Critical 동국대학교 산학협력단
Publication of WO2011065661A2 publication Critical patent/WO2011065661A2/en
Publication of WO2011065661A3 publication Critical patent/WO2011065661A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Cardiology (AREA)
  • Transplantation (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for inducing differentiation of cartilage cells from mesenchymal stem cells, and more particularly, to a method for inducing differentiation of cartilage cells, wherein mesenchymal stem cells are cultured in the presence of DKK-1 or sFRP-1 which serves as a wnt signal transduction inhibitor, and to a treatment composition containing cartilage cells differentiated by said method. The method for differentiating mesenchymal stem cells into cartilage cells according to the present invention uses DKK-1 or sFRP-1 treatment during the early stage of cell culture, so as to prevent differentiation into other cells during the initial cell differentiation of mesenchymal stem cells, thus obtaining cartilage cells in an easier manner.
PCT/KR2010/006837 2009-11-30 2010-10-06 Chondrogenesis of mesenchymal stem cells using dkk-1 or sfrp-1 WO2011065661A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0117288 2009-11-30
KR1020090117288A KR100990436B1 (en) 2009-11-30 2009-11-30 Chondrogenesis of mesenchymal stem cells using dkk-1 or sfrp-1

Publications (2)

Publication Number Publication Date
WO2011065661A2 WO2011065661A2 (en) 2011-06-03
WO2011065661A3 true WO2011065661A3 (en) 2011-09-01

Family

ID=43135926

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/006837 WO2011065661A2 (en) 2009-11-30 2010-10-06 Chondrogenesis of mesenchymal stem cells using dkk-1 or sfrp-1

Country Status (2)

Country Link
KR (1) KR100990436B1 (en)
WO (1) WO2011065661A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2900808B1 (en) 2012-09-28 2019-04-03 Scripps Health Methods of differentiating stem cells into chondrocytes
JP6399558B2 (en) 2012-10-29 2018-10-03 スクリップス ヘルス Method for producing pluripotent stem cells from chondrocytes
EP2911709B1 (en) 2012-10-29 2018-04-18 Scripps Health Methods of transplanting chondrocytes
KR102197871B1 (en) * 2019-03-28 2021-01-04 이화여자대학교 산학협력단 Method for inducing differentiation stem cells into chondrocytes using folic acid, folic acid derivatives or antifolates

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007267658A (en) * 2006-03-31 2007-10-18 Cardio Corp Method for producing myocardial cell and differentiation induction accelerator to myocardial cell
KR20090069013A (en) * 2007-12-24 2009-06-29 동국대학교 산학협력단 Chondrogenesis of bone marrow mesenchymal stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007267658A (en) * 2006-03-31 2007-10-18 Cardio Corp Method for producing myocardial cell and differentiation induction accelerator to myocardial cell
KR20090069013A (en) * 2007-12-24 2009-06-29 동국대학교 산학협력단 Chondrogenesis of bone marrow mesenchymal stem cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HUANG, C.Y. ET AL.: "Chondrogenesis of human bone marrow-derived mesenchymal stem cells in agarose culture", THE ANATOMICAL RECORD PART A, vol. 278, no. 1, May 2004 (2004-05-01), pages 428 - 436, XP009085545, DOI: doi:10.1002/ar.a.20010 *
IM, GUN-IL ET AL.: "The Effects ofWnt Inhibitors on the Chondrogenesis of Human Mesenchymal Stem Cells", TISSUE ENGINEERING PART A, vol. 16, no. 7, 8 September 2009 (2009-09-08), pages 2405 - 2413 *

Also Published As

Publication number Publication date
WO2011065661A2 (en) 2011-06-03
KR100990436B1 (en) 2010-10-29

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