WO2011057710A3 - Microorganisms having enhanced sucrose mutase activity - Google Patents

Microorganisms having enhanced sucrose mutase activity Download PDF

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Publication number
WO2011057710A3
WO2011057710A3 PCT/EP2010/006487 EP2010006487W WO2011057710A3 WO 2011057710 A3 WO2011057710 A3 WO 2011057710A3 EP 2010006487 W EP2010006487 W EP 2010006487W WO 2011057710 A3 WO2011057710 A3 WO 2011057710A3
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
mutase activity
sucrose mutase
enhanced sucrose
enhanced
Prior art date
Application number
PCT/EP2010/006487
Other languages
German (de)
French (fr)
Other versions
WO2011057710A2 (en
Inventor
Stefan Pelzer
Christian Zurek
Thomas Rose
Jürgen Eck
Wolfgang Wach
Michael Klingeberg
Karsten Harms
Original Assignee
Südzucker Aktiengesellschaft Mannheim/Ochsenfurt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Südzucker Aktiengesellschaft Mannheim/Ochsenfurt filed Critical Südzucker Aktiengesellschaft Mannheim/Ochsenfurt
Priority to AU2010318324A priority Critical patent/AU2010318324B2/en
Priority to EP10768885.5A priority patent/EP2499253B1/en
Priority to ES10768885.5T priority patent/ES2543167T3/en
Priority to KR1020127014948A priority patent/KR101736245B1/en
Priority to BR112012011235A priority patent/BR112012011235A2/en
Priority to US13/509,250 priority patent/US8790900B2/en
Publication of WO2011057710A2 publication Critical patent/WO2011057710A2/en
Publication of WO2011057710A3 publication Critical patent/WO2011057710A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99011—Isomaltulose synthase (5.4.99.11)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The invention relates to the biotechnological production of isomaltulose and isomaltulose-containing compositions and to improved agents, in particular microbial cells, for said purpose.
PCT/EP2010/006487 2009-11-11 2010-10-23 Microorganisms having enhanced sucrose mutase activity WO2011057710A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2010318324A AU2010318324B2 (en) 2009-11-11 2010-10-23 Microorganisms having enhanced sucrose mutase activity
EP10768885.5A EP2499253B1 (en) 2009-11-11 2010-10-23 Microorganisms having enhanced sucrose mutase activity
ES10768885.5T ES2543167T3 (en) 2009-11-11 2010-10-23 Microorganisms with increased sucrose mutase activity
KR1020127014948A KR101736245B1 (en) 2009-11-11 2010-10-23 Microorganisms having enhanced sucrose mutase activity
BR112012011235A BR112012011235A2 (en) 2009-11-11 2010-10-23 microorganisms with enhanced sucrose mutase activity
US13/509,250 US8790900B2 (en) 2009-11-11 2010-10-23 Microorganisms having enhanced sucrose mutase activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009053566.7 2009-11-11
DE102009053566.7A DE102009053566B4 (en) 2009-11-11 2009-11-11 Microorganisms with increased Sucrosemutaseaktivität

Publications (2)

Publication Number Publication Date
WO2011057710A2 WO2011057710A2 (en) 2011-05-19
WO2011057710A3 true WO2011057710A3 (en) 2011-09-15

Family

ID=43530436

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/006487 WO2011057710A2 (en) 2009-11-11 2010-10-23 Microorganisms having enhanced sucrose mutase activity

Country Status (8)

Country Link
US (1) US8790900B2 (en)
EP (1) EP2499253B1 (en)
KR (1) KR101736245B1 (en)
AU (1) AU2010318324B2 (en)
BR (1) BR112012011235A2 (en)
DE (1) DE102009053566B4 (en)
ES (1) ES2543167T3 (en)
WO (1) WO2011057710A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009060935A1 (en) 2009-12-23 2011-06-30 Südzucker AG Mannheim/Ochsenfurt, 68165 Sucrosemutase with improved product specificity
US10745675B2 (en) 2017-05-02 2020-08-18 The United States Of America, As Represented By The Secretary Of Agriculture Modified enzymes for producing increased isomelezitose

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030203468A1 (en) * 1994-01-19 2003-10-30 Sudzucker Aktiengesellschaft Preparation of acariogenic sugar substitutes
WO2009152480A2 (en) * 2008-06-13 2009-12-17 Vivocure, Inc. Methods to treat solid tumors

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0001099B1 (en) * 1977-09-13 1980-08-20 Bayer Ag Process for the continuous isomerisation if saccharose to isomaltulose by means of microorganisms
SE9203753D0 (en) * 1992-12-11 1992-12-11 Kabi Pharmacia Ab EXPRESSION SYSTEM FOR PRODUCING APOLIPOPROTEIN AI-M
DE4447471C2 (en) * 1994-01-19 1995-12-21 Suedzucker Ag Protein with palatinase activity and nucleic acid coding therefor
DE19523560A1 (en) 1995-06-28 1997-01-02 Suedzucker Ag Sucrose metabolism mutants
AUPQ976800A0 (en) * 2000-08-29 2000-09-21 University Of Queensland, The Novel polypeptides and polynucleotides and uses therefor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030203468A1 (en) * 1994-01-19 2003-10-30 Sudzucker Aktiengesellschaft Preparation of acariogenic sugar substitutes
WO2009152480A2 (en) * 2008-06-13 2009-12-17 Vivocure, Inc. Methods to treat solid tumors

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ALPER HAL ET AL: "Tuning genetic control through promoter engineering", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (PNAS), NATIONAL ACADEMY OF SCIENCE, US, vol. 102, no. 36, 6 September 2005 (2005-09-06), pages 12678 - 12683, XP002428722, ISSN: 0027-8424, DOI: DOI:10.1073/PNAS.0504604102 *
CHA J ET AL: "Molecular cloning and functional characterization of a sucrose isomerase (isomaltulose synthase) gene from Enterobacter sp FMB-1", JOURNAL OF APPLIED MICROBIOLOGY, vol. 107, no. 4, October 2009 (2009-10-01), pages 1119 - 1130, XP002622270, ISSN: 1364-5072 *
DATABASE Geneseq [online] 4 March 2010 (2010-03-04), "S. enterica 3,4 dihydroxy-2-butanone-4-phosphate synthase promoter.", XP002622272, retrieved from EBI accession no. GSN:AXU25741 Database accession no. AXU25741 *
DE OLIVA-NETO P ET AL: "Isomaltulose production from sucrose by Protaminobacter rubrum immobilized in calcium alginate", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 100, no. 18, 1 September 2009 (2009-09-01), pages 4252 - 4256, XP026130078, ISSN: 0960-8524, [retrieved on 20090501], DOI: DOI:10.1016/J.BIORTECH.2009.03.060 *
HERRERO M ET AL: "TRANSPOSON VECTORS CONTAINING NON-ANTIBIOTIC RESISTANCE SELECTION MARKERS FOR CLONING AND STABLE CHROMOSOMAL INSERTION OF FOREIGN GENES IN GRAM-NETATIVE BACTERIA", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 172, no. 11, 1 November 1990 (1990-11-01), pages 6557 - 6567, XP000572232, ISSN: 0021-9193 *
RICHTER G ET AL: "BIOSYNTHESIS OF RIBOFLAVIN CLONING SEQUENCING AND EXPRESSION OF THE GENE CODING FOR 3 4 DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE OF ESCHERICHIA-COLI", JOURNAL OF BACTERIOLOGY, vol. 174, no. 12, 1992, pages 4050 - 4056, XP002622271, ISSN: 0021-9193 *
SAWITZKE JAMES A ET AL: "Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond", METHODS IN ENZYMOLOGY; [METHODS IN ENZYMOLOGY], ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 421, 1 January 2007 (2007-01-01), pages 171 - 199, XP008124026, ISSN: 0076-6879, [retrieved on 20070309], DOI: DOI:10.1016/S0076-6879(06)21015-2 *
WILLIAM R MCCLEARY: "Application of promoter swapping techniques to control expression of chromosomal genes", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 84, no. 4, 25 July 2009 (2009-07-25), pages 641 - 648, XP019737788, ISSN: 1432-0614, DOI: DOI:10.1007/S00253-009-2137-Y *

Also Published As

Publication number Publication date
DE102009053566A1 (en) 2011-05-12
US8790900B2 (en) 2014-07-29
KR20120095962A (en) 2012-08-29
KR101736245B1 (en) 2017-05-29
AU2010318324B2 (en) 2014-09-25
DE102009053566B4 (en) 2014-08-14
US20120225458A1 (en) 2012-09-06
EP2499253A2 (en) 2012-09-19
EP2499253B1 (en) 2015-04-22
AU2010318324A1 (en) 2012-05-24
WO2011057710A2 (en) 2011-05-19
ES2543167T3 (en) 2015-08-17
BR112012011235A2 (en) 2015-09-29

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