WO2011002957A3 - Droplet actuator devices and methods - Google Patents

Droplet actuator devices and methods Download PDF

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Publication number
WO2011002957A3
WO2011002957A3 PCT/US2010/040705 US2010040705W WO2011002957A3 WO 2011002957 A3 WO2011002957 A3 WO 2011002957A3 US 2010040705 W US2010040705 W US 2010040705W WO 2011002957 A3 WO2011002957 A3 WO 2011002957A3
Authority
WO
WIPO (PCT)
Prior art keywords
provides
another embodiment
droplet
actuators
actuator devices
Prior art date
Application number
PCT/US2010/040705
Other languages
French (fr)
Other versions
WO2011002957A2 (en
Inventor
Michael G. Pollack
Vijay Srinivasan
Vamsee K. Pamula
George Brackett
Keith R. Brafford
Darius Clark
Zhishan Hua
Scott Norton
Ramakrishna Sista
Gregory F. Smith
Ryan A. Sturmer
Arjun Sudarsan
Prasanna Thwar
Tih-Hong Wang
Theodore Winger
Original Assignee
Advanced Liquid Logic, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic, Inc. filed Critical Advanced Liquid Logic, Inc.
Publication of WO2011002957A2 publication Critical patent/WO2011002957A2/en
Publication of WO2011002957A3 publication Critical patent/WO2011002957A3/en
Priority to US13/238,872 priority Critical patent/US8926065B2/en
Priority to US14/580,407 priority patent/US9545640B2/en
Priority to US14/870,433 priority patent/US9545641B2/en
Priority to US15/099,175 priority patent/US9707579B2/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01F33/30—Micromixers
    • B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/00—Additional constructional details
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Coating Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides droplet actuator devices for facilitating certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuators that facilitate droplet operations in three dimensions. In another embodiment, the invention provides for cost-effective production of droplet actuators. In another embodiment, the invention provides for replacing one or more components of a droplet actuator. In another embodiment, the invention provides droplet actuators using printed conductive inks to form electrodes and/or ground planes. In another embodiment, the invention provides a magnetic clamping fixture for assembling droplet actuators. In another embodiment, the invention provides simple, low cost power sources for use in combination with microfluidic systems. In another embodiment, the invention provides an immunoassay multiplexing platform that uses digital microfluidics and real-time imaging of flash-based chemiluminescent signals. In still another embodiment, the invention provides droplet actuator devices that are fabricated using a plasma treatment process to raise substrate surface energy.
PCT/US2010/040705 2009-07-01 2010-07-01 Droplet actuator devices and methods WO2011002957A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US13/238,872 US8926065B2 (en) 2009-08-14 2011-09-21 Droplet actuator devices and methods
US14/580,407 US9545640B2 (en) 2009-08-14 2014-12-23 Droplet actuator devices comprising removable cartridges and methods
US14/870,433 US9545641B2 (en) 2009-08-14 2015-09-30 Droplet actuator devices and methods
US15/099,175 US9707579B2 (en) 2009-08-14 2016-04-14 Droplet actuator devices comprising removable cartridges and methods

Applications Claiming Priority (22)

Application Number Priority Date Filing Date Title
US22218509P 2009-07-01 2009-07-01
US61/222,185 2009-07-01
US22558209P 2009-07-15 2009-07-15
US61/225,582 2009-07-15
US23411409P 2009-08-14 2009-08-14
US61/234,114 2009-08-14
US23700809P 2009-08-26 2009-08-26
US61/237,008 2009-08-26
US23851209P 2009-08-31 2009-08-31
US61/238,512 2009-08-31
US24154309P 2009-09-11 2009-09-11
US61/241,543 2009-09-11
US25484909P 2009-10-26 2009-10-26
US25487709P 2009-10-26 2009-10-26
US25486509P 2009-10-26 2009-10-26
US61/254,849 2009-10-26
US61/254,865 2009-10-26
US61/254,877 2009-10-26
US28866509P 2009-12-21 2009-12-21
US61/288,665 2009-12-21
US29487410P 2010-01-14 2010-01-14
US61/294,874 2010-01-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/238,872 Continuation-In-Part US8926065B2 (en) 2009-08-14 2011-09-21 Droplet actuator devices and methods

Publications (2)

Publication Number Publication Date
WO2011002957A2 WO2011002957A2 (en) 2011-01-06
WO2011002957A3 true WO2011002957A3 (en) 2011-08-04

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Family Applications (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5897780B2 (en) 2005-01-28 2016-03-30 デューク ユニバーシティ Apparatus and method for operating droplets on a printed circuit board
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US8872527B2 (en) 2007-02-15 2014-10-28 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
CH700127A1 (en) 2008-12-17 2010-06-30 Tecan Trading Ag System and apparatus for processing biological samples and for manipulating liquids with biological samples.
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
EP2516669B1 (en) 2009-12-21 2016-10-12 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
WO2011126892A2 (en) 2010-03-30 2011-10-13 Advanced Liquid Logic, Inc. Droplet operations platform
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
EP2588322B1 (en) 2010-06-30 2015-06-17 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
WO2012151192A2 (en) * 2011-05-02 2012-11-08 Advanced Liquid Logic, Inc. Molecular diagnostics platform
WO2012154745A2 (en) 2011-05-09 2012-11-15 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
CA2833907A1 (en) 2011-05-10 2012-11-15 Advanced Liquid Logic, Inc. Enzyme concentration and assays
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
KR20140064771A (en) 2011-07-06 2014-05-28 어드밴스드 리퀴드 로직, 아이엔씨. Reagent storage on a droplet actuator
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
WO2013066441A2 (en) * 2011-07-29 2013-05-10 The Texas A&M University System Digital microfluidic platform for actuating and heating individual liquid droplets
WO2013040562A2 (en) * 2011-09-15 2013-03-21 Advanced Liquid Logic Inc Microfluidic loading apparatus and methods
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
US8821705B2 (en) 2011-11-25 2014-09-02 Tecan Trading Ag Digital microfluidics system with disposable cartridges
US9377439B2 (en) 2011-11-25 2016-06-28 Tecan Trading Ag Disposable cartridge for microfluidics system
US10724988B2 (en) 2011-11-25 2020-07-28 Tecan Trading Ag Digital microfluidics system with swappable PCB's
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
BR112014032727B1 (en) 2012-06-27 2021-12-14 Illumina France METHOD AND SYSTEM FOR PERFORMING DROP OPERATIONS ON A DROPOLE ON A DROP ACTUATOR TO REDUCE BUBBLE FORMATION
WO2014062551A1 (en) * 2012-10-15 2014-04-24 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
US20140151229A1 (en) * 2012-12-05 2014-06-05 Caliper Life Sciences, Inc. Manipulation of objects in microfluidic devices using external electrodes
EP2869922B1 (en) 2013-01-09 2019-11-20 Tecan Trading AG Disposable cartridge for microfluidics systems
US9630176B2 (en) 2013-01-09 2017-04-25 Tecan Trading Ag Microfluidics systems with waste hollow
WO2014108218A1 (en) 2013-01-09 2014-07-17 Tecan Trading Ag Microfluidics systems with waste hollow
CN105008052B (en) 2013-03-04 2018-09-14 泰肯贸易股份公司 The manipulation of droplet size in digital micro-fluid system
CN104321141B (en) 2013-05-23 2017-09-22 泰肯贸易股份公司 Digital micro-fluid system with interconvertible PCB
US20160116438A1 (en) * 2013-06-14 2016-04-28 Advanced Liquid Logic, Inc. Droplet actuator and methods
AU2014284148B2 (en) 2013-06-21 2017-02-23 Bio-Rad Laboratories, Inc. Microfluidic system with fluid pickups
EP3033599A4 (en) 2013-08-13 2017-03-22 Advanced Liquid Logic, Inc. Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input
JP2016539343A (en) 2013-08-30 2016-12-15 イルミナ インコーポレイテッド Manipulating droplets on hydrophilic or mottled hydrophilic surfaces
DE102014100871B4 (en) 2014-01-27 2016-11-17 Karlsruher Institut für Technologie Digital microfluidic platform
US8820538B1 (en) * 2014-03-17 2014-09-02 Namocell LLC Method and apparatus for particle sorting
CN103932369B (en) * 2014-04-09 2015-08-26 西安交通大学 A kind of controlled jetting type food 3D printing device and method
CN103932368B (en) * 2014-04-09 2015-10-28 西安交通大学 A kind of laser 3D food printing equipment and Method of printing
CN106457827B (en) 2014-04-16 2018-05-08 雅培制药有限公司 Droplet actuator manufacture device, system and correlation technique
EP3680333A1 (en) 2014-04-29 2020-07-15 Illumina, Inc. Multiplexed single cell expression analysis using template switch and tagmentation
US10137450B2 (en) 2014-07-18 2018-11-27 Tecan Trading Ag Microfluidics cartridge with pipetting guide
WO2016057950A1 (en) 2014-10-09 2016-04-14 Illumina, Inc. Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
JP6622811B2 (en) 2014-12-31 2019-12-18 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ Digital microfluidic diluter, system and related methods
CA2975739C (en) 2015-02-10 2022-12-06 Illumina, Inc. Methods and compositions for analyzing cellular components
CN107847930B (en) * 2015-03-20 2020-06-30 亿明达股份有限公司 Fluid cartridges for use in a vertical or substantially vertical position
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
EP3303548A4 (en) 2015-06-05 2019-01-02 Miroculus Inc. Evaporation management in digital microfluidic devices
US10857537B2 (en) 2015-07-06 2020-12-08 Illumina, Inc. Balanced AC modulation for driving droplet operations electrodes
WO2017030999A1 (en) 2015-08-14 2017-02-23 Illumina, Inc. Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
EP4368715A2 (en) 2015-08-28 2024-05-15 Illumina, Inc. Nucleic acid sequence analysis from single cells
CN109153016B (en) 2015-09-02 2021-12-28 帝肯贸易股份公司 Magnetic conduits in microfluids
WO2017037078A1 (en) * 2015-09-02 2017-03-09 Illumina Cambridge Limited Systems and methods of improving droplet operations in fluidic systems
US10377538B2 (en) 2015-12-01 2019-08-13 Illumina, Inc. Liquid storage and delivery mechanisms and methods
ES2875759T3 (en) 2015-12-01 2021-11-11 Illumina Inc Digital microfluidic system for single cell isolation and analyte characterization
US10241319B2 (en) * 2015-12-28 2019-03-26 Amazon Technologies, Inc. Electrowetting display pixels with fluid motion initiator
EP3440220B1 (en) 2016-04-07 2020-03-25 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
CN106053467B (en) * 2016-06-08 2019-02-19 中国科学院上海微***与信息技术研究所 A kind of device and method for observing microlayer model
US10596572B2 (en) 2016-08-22 2020-03-24 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
CA3049416A1 (en) 2016-12-28 2018-07-05 Miroculus Inc. Digital microfluidic devices and methods
CN110505918B (en) 2017-02-13 2022-06-14 生物辐射实验室股份有限公司 Systems, methods, and apparatus for forming an emulsion array
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
WO2018194646A1 (en) * 2017-04-21 2018-10-25 Hewlett-Packard Development Company, L.P. Electrowetting force droplet manipulation
GR20170100305A (en) * 2017-06-30 2019-03-20 Εθνικο Κεντρο Ερευνας Φυσικων Επιστημων (Εκεφε) " Δημοκριτος" Microfluidic reactors and process for their production
CN110892258A (en) 2017-07-24 2020-03-17 米罗库鲁斯公司 Digital microfluidic system and method with integrated plasma collection device
EP3676009A4 (en) * 2017-09-01 2021-06-16 Miroculus Inc. Digital microfluidics devices and methods of using them
WO2019157170A1 (en) * 2018-02-07 2019-08-15 The Trustees Of The University Of Pennsylvania High throughput microfluidic device
WO2019245525A1 (en) * 2018-06-18 2019-12-26 Hewlett-Packard Development Company, L.P. Microfluidic immunoassays
DE102019202174A1 (en) * 2019-02-19 2020-08-20 Robert Bosch Gmbh Device for examining a biological sample
US20220126256A1 (en) * 2019-02-25 2022-04-28 National Institute Of Advanced Industrial Science And Technology Open-type liquid manipulation device
EP3930901A4 (en) * 2019-02-28 2023-01-04 Miroculus Inc. Digital microfluidics devices and methods of using them
AU2020242931A1 (en) * 2019-03-21 2021-11-04 Nicoya Lifesciences, Inc. Foldable digital microfluidic (DMF) device using flexible electronic platform and methods of making same
US11738345B2 (en) 2019-04-08 2023-08-29 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CN110341309B (en) * 2019-08-15 2020-11-27 昆山国显光电有限公司 Ink-jet printing device
JP2023511519A (en) * 2020-01-22 2023-03-20 ニコヤ ライフサイエンシーズ インコーポレイテッド Methods Including Digital Microfluidic Systems, Cartridges, and Integrated Refractive Index Sensing
CN112344879B (en) * 2020-09-29 2022-03-25 联想(北京)有限公司 Method, device and equipment for detecting glue road
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
WO2023235989A1 (en) * 2022-06-09 2023-12-14 Nicoya Lifesciences, Inc. Colored coverlay dielectrics in digital microfluidics

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040055536A1 (en) * 2002-09-24 2004-03-25 Pramod Kolar Method and apparatus for non-contact electrostatic actuation of droplets
US20040058450A1 (en) * 2002-09-24 2004-03-25 Pamula Vamsee K. Methods and apparatus for manipulating droplets by electrowetting-based techniques
US6773566B2 (en) * 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US20060226013A1 (en) * 2002-12-18 2006-10-12 Decre Michel M J Manipulation of objects with fluid droplets
US7531072B2 (en) * 2004-02-16 2009-05-12 Commissariat A L'energie Atomique Device for controlling the displacement of a drop between two or several solid substrates

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773566B2 (en) * 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US20040055536A1 (en) * 2002-09-24 2004-03-25 Pramod Kolar Method and apparatus for non-contact electrostatic actuation of droplets
US20040058450A1 (en) * 2002-09-24 2004-03-25 Pamula Vamsee K. Methods and apparatus for manipulating droplets by electrowetting-based techniques
US20060226013A1 (en) * 2002-12-18 2006-10-12 Decre Michel M J Manipulation of objects with fluid droplets
US7531072B2 (en) * 2004-02-16 2009-05-12 Commissariat A L'energie Atomique Device for controlling the displacement of a drop between two or several solid substrates

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
POLLACK,M. G. ET AL.: "Electrowetting-based actuation of droplets for integrated microfluidics.", LAB ON A CHIP., 2002, pages 96 - 101, XP008038786, DOI: doi:10.1039/b110474h *
ROUX, JEAN-MAXIME ET AL.: "3D droplet displacement in microfluidic system by electrostatic actuation.", SENSORS AND ACTUATORS A., vol. 134, March 2007 (2007-03-01), pages 486 - 493, XP055135530, DOI: doi:10.1016/j.sna.2006.05.012 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use

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