WO2010123205A2 - Constant temperature agitating device and agitating method using the same - Google Patents
Constant temperature agitating device and agitating method using the same Download PDFInfo
- Publication number
- WO2010123205A2 WO2010123205A2 PCT/KR2010/001792 KR2010001792W WO2010123205A2 WO 2010123205 A2 WO2010123205 A2 WO 2010123205A2 KR 2010001792 W KR2010001792 W KR 2010001792W WO 2010123205 A2 WO2010123205 A2 WO 2010123205A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- container
- body portion
- contact
- lower body
- upper body
- Prior art date
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/201—Holders therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/22—Mixing the contents of independent containers, e.g. test tubes with supporting means moving in a horizontal plane, e.g. describing an orbital path for moving the containers about an axis which intersects the receptacle axis at an angle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/90—Heating or cooling systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/90—Heating or cooling systems
- B01F35/92—Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/90—Heating or cooling systems
- B01F2035/99—Heating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
Definitions
- the present invention relates to a stirring method using a constant temperature stirrer and a constant temperature stirrer, and more particularly, to a stirring method using a constant temperature stirrer and a constant temperature stirrer for efficiently stirring the water contained in the container.
- Enzymes or chemicals can be added to a sample taken from an animal or human body to extract a desired substance from the sample taken or to observe the reaction of the sample to enzymes or chemicals.
- the sample collected below may be referred to as a target substance, and enzymes or chemicals that react with the target substance may be referred to as reactants, and both the target substance and the reactants may be referred to as water-soluble substances.
- the container may be provided in the form of a bag made of a flexible material for accommodating the receiving material, or may be provided in a test tube or a petri dish having a certain shape.
- the water contained in the container may be heated or adjusted to a predetermined temperature and then maintained at that temperature for a predetermined time.
- the container may be shaken up, down, left, or right, or vibrated to mix the target and reactant well to induce an effective reaction.
- the warming and constant temperature process for the receiving material may be accomplished through various methods.
- the container containing the receiving material may be warmed or maintained at a constant temperature using a heat transfer method through air.
- the vessel itself containing the receiving material may be warmed or maintained at a constant temperature bath using heat transfer through water.
- the heat transfer method through the air using a thermostat takes a relatively long time to heat the vessel to a predetermined temperature, the time that the receiving material reaches the target enzyme reaction temperature of 37 °C using the warmed circulating air Due to the large amount required, damage to the sample can be prevented, and the preparation of the desired material may not be easy.
- the heat transfer method through the water using a constant temperature water tank can heat the vessel relatively faster than the heat transfer through the air described above, but the container is in direct contact with water and the bacteria and Exposure to external factors such as viruses can easily occur.
- the present invention provides a stirring method using a constant temperature stirrer and a constant temperature stirrer capable of heating the water contained in the container to a predetermined temperature in a relatively fast time and maintaining a constant temperature.
- the present invention provides a stirring method using a constant temperature stirrer and a constant temperature stirrer capable of isolating the water contained in the container from the contaminants present in the water tank, heating and maintaining a constant temperature in a short time.
- the constant temperature stirring device for stirring the receiving material contained in the container is located on the base, the upper part of the base to cover the upper and lower parts of the container An inner surface of the upper body portion and the lower body portion, the upper body portion and the lower body portion in contact with the container, the container receiving body portion forming a container receiving portion corresponding to the outer surface of the container, and shaking the container receiving body portion relative to the base It is provided with a shaking motion to exercise, the heat transfer to the container through the inner surface of the container receiving portion in contact with the container.
- the container receiving part may be formed through various methods.
- at least one of the upper body part and the lower body part may include a solid mold in contact with the container, and the solid mold may be formed to correspond to the outer surface of the container. It includes a groove, the receiving groove may form part of the container receiving portion.
- At least one of the upper body portion and the lower body portion includes a flow pack in contact with the container, the inside of the flow pack is filled with a fluid capable of storing heat, the outer surface of the flow pack to the outer surface of the container It may be deformed to be in close contact to form part of the container receiving portion.
- the flow pack may be formed of a material capable of elastic deformation.
- At least one of the upper body portion and the lower body portion includes a plurality of contact adhesive pieces contacting the container, and the contact adhesive pieces are supported to be displaceable with respect to the upper body portion or the lower body portion, and the contact closely adheres to each other.
- the piece may be displaced to be in close contact with the outer surface of the container and form part of the container receiving portion.
- At least one of the upper body portion and the lower body portion includes a heating element, it is possible to transfer the heat generated from the heating element to the container, the heating element is preferably disposed adjacent to the container receiving portion.
- the container receiving body portion for the stirring device for agitating the receiving material contained in the container coupled to the shaking motion base capable of shaking motion, the upper body to accommodate the upper and lower parts of the container
- An inner surface of the upper body portion and the lower body portion which includes a portion and a lower body portion, and forms a container receiving portion corresponding to the outer surface of the container, and may transfer heat to the container through the inner surface of the container receiving portion in contact with the container.
- Container housing body portion according to the present invention can be detachably combined with the base or shaking motion portion of the constant temperature stirring device, can be used to replace the container housing body portion to match the container provided in various forms.
- the base may be provided separately from the shaking motion part, and may refer to the upper surface of the shaking motion part.
- a stirring method for agitating a receiving material contained in a container using a stirring device includes an upper body portion and a lower body portion covering the upper and lower portions of the container, and inner surfaces of the upper body portion and the lower body portion in contact with the container include a container accommodating body portion forming a container accommodating portion corresponding to the outer surface of the container.
- the constant temperature stirring device and the stirring method of the present invention can minimize the damage to the sample by heating the water contained in the container to a desired temperature in a relatively fast time, the production of the desired material can be made efficiently.
- the constant temperature stirrer and the stirring method of the present invention can be isolated from the contaminants present in the water bath, can be heated and maintained at a constant time, and at the same time can agitate the receiving material.
- FIG. 1 is a perspective view showing a constant temperature stirring device according to a first embodiment of the present invention.
- FIG. 2 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of FIG. 1 in the A-A direction.
- Figure 3 is a partial perspective view showing a constant temperature stirring device according to a second embodiment of the present invention.
- FIG. 4 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of FIG. 3 in the B-B direction.
- Figure 5 is a perspective view of the container housing body portion of the constant temperature stirring device according to a third embodiment of the present invention.
- FIG. 6 is a cross-sectional view illustrating the container housing body part by cutting FIG. 5 in the C-C direction.
- FIG. 1 is a perspective view showing a constant temperature stirring device according to a first embodiment of the present invention
- Figure 2 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of Figure 1 in the A-A direction.
- the constant temperature agitation device 100 for agitating the receiving material contained in the container 10 includes a base 110, a container housing body 120, and a shaking motion part 150.
- Stem cells of adipose tissue can be used for the purpose of regenerating various damaged tissues in the human body, and in particular, they can be differentiated into hematopoietic cells, nerve cells, skin reconstructions, chondrocytes, cardiomyocytes, and blood vessel cells. Can be used.
- stem cells when stem cells are separated from bone marrow, umbilical cord blood, peripheral blood, and embryos, it is necessary to overcome complex problems such as ethical issues, pain of the subjects to be collected, and contamination by external factors such as bacteria and viruses during the process. The usefulness of stem cells is becoming more important.
- a constant temperature agitation device using the excess adipose tissue in the human body as a sample, it can be used to isolate the adipose stem cells described above.
- the sample collected in the present specification may be referred to as a target substance
- enzymes or chemicals reacting with the target substance may be referred to as a reactant
- both the target substance and the reactant may be referred to as water-soluble substances.
- the container 10 is provided in this embodiment in the form of a bag made of a flexible material to accommodate the receiving material.
- the base 110 is provided in a plate shape, the bottom surface of the base 110 is connected to the output shaft 151 of the gear assembly for transforming the rotational movement of the motor disposed in the shaking motion unit 150 to the up, down, left and right and vibration movement.
- the base 110 is coupled to the lower body portion 125 of the container receiving body portion 120.
- a pair of hook portions 111 extending upward from the side edges of the base 110 are formed, and a pair of coupling grooves in which the hook portions 111 are inserted into both side surfaces of the lower body portion 125 is formed. 129 is formed.
- the container housing body 120 may be detachably coupled to the base 110.
- the container accommodating body part 120 is positioned above the base 110 and includes an upper body part 121 and a lower body part 125 that cover and accommodate the upper and lower parts of the container 10, and contact the container 10.
- the inner surfaces of the upper body portion 121 and the lower body portion 125 form a container receiving portion 130 corresponding to the outer surface of the container 10, the container 10 is an inner surface in contact with the container receiving portion 130 Heat can be transmitted through.
- the upper body portion 121 includes an upper outer case 122, an upper intermediate mold 123, and an upper inner mold 124.
- the upper outer case 122 is formed using a material such as synthetic resin and rubber having low thermal conductivity, so that the heat of the heating element 160 disposed inside the upper outer case 122 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 10 at warm and constant temperature.
- the upper intermediate mold 123 is formed to be inserted into the upper outer case 122, and a space in which the heating element 160 is disposed is provided.
- the upper inner mold 124 is inserted into the arrangement space provided in the upper intermediate mold 123, the upper receiving grooves 131 are formed in the upper surface of each container 10 in contact with the inner surface.
- the upper inner mold 124 may be used to replace the upper receiving groove 131 is formed to match the outer shape of the container 10 that can be provided in various forms.
- the upper middle mold 123 and the upper inner mold 124 are formed of a metal mold having excellent thermal conductivity, and the heat generated from the heating element 160 is easily conducted, and the container is inserted into the upper receiving groove 131 ( 10) can conduct heat evenly.
- the lower body portion 125 includes a lower outer case 126, a lower intermediate mold 127, and a lower inner mold 128.
- the lower outer case 126 is formed using a material such as synthetic resin and rubber having a low thermal conductivity, so that the heat of the heating element 160 that is disposed inside the lower outer case 126 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 10 at warm and constant temperature.
- the lower intermediate mold 127 is formed to be inserted into the lower outer case 126, and a space in which the heating element 160 is disposed is provided.
- the lower inner mold 128 is inserted into an arrangement space provided in the lower intermediate mold 127, and lower receiving grooves 132 are formed at upper portions of the respective containers 10 to contact their inner surfaces.
- the lower inner mold 128 may be used to replace the lower receiving groove 132 is formed to match the outer shape of the container 10 that can be provided in various forms.
- the lower middle mold 127 and the lower inner mold 128 are formed of a metal mold having excellent thermal conductivity, and heat generated from the heating element 160 is easily conducted, and the container is inserted into the lower receiving groove 132. 10) can conduct heat evenly.
- the lower outer case 126 may be provided with a temperature display window that can display the temperature separately from the temperature window provided on the outside of the shaking motion unit 150 to be described later, the shaking motion unit 150 or the lower outer case
- the temperature windows provided at 126 may all be disposed around the vessel 10 or the heating element 160 to display the temperature through a temperature sensor for measuring the temperature.
- the unit 130 is formed.
- the heating element 160 is disposed in each of the upper intermediate mold 123 and the lower intermediate mold 127, but in some cases it may be installed only on either side. However, the heating element 160 may be disposed adjacent to the container receiving portion 130 for effective heat conduction.
- the upper body 121 and the lower body 125 is foldably coupled so that the upper body 121 is relatively movable with the lower body 125 so as to expose the upper surface of the lower body 125. To be combined.
- the container 10 is disposed in the lower receiving groove 132 formed in the lower body portion 125 while the upper body 121 is opened to open the front surface of the lower body portion 125, and the upper body portion ( The container 10 may be disposed in the container accommodating part 130 by closing the front surface of the lower body part 125.
- the shaking motion unit 150 may shake the container housing body 120 with respect to the base 110, and the shaking motion may include both up, down, left, and right movements or vibration movements.
- the fixing member 140 is provided in the form of a clip
- the upper body portion 121 and the lower body portion 125 may be coupled to each other.
- the appropriate temperature of the container 10 while performing a shaking motion so that the mixing between the target material and the reactant contained in the container 10 is well made in this embodiment the temperature of the water to the optimum temperature of the target enzyme reaction 37 °C It can be raised to maintain its temperature for a period of time.
- the shaking motion unit 150 controls the rotation speed of the motor and the gearbox and the motor to be transmitted to the base 110 by transforming the rotational motion of the motor and the rotational movement of the motor up and down, left and right and vibration, It may include a control unit for controlling the power delivered to the heating element 160 provided as a heating wire.
- the power provided from the shaking motion unit 150 to the heating element 160 may be transmitted through the output shaft 151.
- the power delivered to the heating element may be supplied from a battery or a separate AC power provided separately in the upper or lower body portion in which the heating element is disposed.
- Heat transmitted directly from the heating element 160 to the container 10 may instantly raise the temperature of the container 10 in contact with the upper body 121 and the lower body 125 to a higher temperature than the target temperature, but with stirring.
- the shaking action proceeds at the same time, the effect on the actual cells is insignificant and the enzyme treatment can proceed quickly without damaging the cells.
- the method of transferring heat to the container 10 through the inner surface of the container accommodating part 120 as compared with the transfer of heat through a conventional thermostat or a constant temperature bath, heat directly through the constant temperature water tank It achieves heat transfer efficiency similar to that of transfer, but can be isolated from the contaminants present in the bath because it is not water-borne.
- the constant temperature stirring device according to the second embodiment of the present invention is substantially the same as the constant temperature stirring device described in the first embodiment. Therefore, the description of the constant temperature stirring apparatus I in the second embodiment may refer to the description and drawings of the constant temperature stirring apparatus of the first embodiment, and the repeated content may be omitted.
- the shaking motion portion and the base of the constant temperature stirring device of the present embodiment may be used substantially the same as the shaking motion portion described in the first embodiment, the contents of the shaking motion portion and the base of the present embodiment described in the first embodiment and See description.
- Figure 3 is a partial perspective view showing a constant temperature stirring device according to a second embodiment of the present invention
- Figure 4 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of Figure 3 in the B-B direction.
- the container 20 is provided as a test tube having a certain shape to accommodate the receiving material in this embodiment.
- the container accommodating body part 220 is positioned above the base 110 and includes an upper body part 221 and a lower body part 225 that cover and accommodate the upper and lower parts of the container 20, and are in contact with the container 20.
- the inner surfaces of the upper body portion 221 and the lower body portion 225 form a container receiving portion 230 corresponding to the outer surface of the container 20, the container 20 is the inner surface in contact with the container receiving portion 230 Heat can be transmitted through.
- the upper body portion 221 includes an upper outer case 222 and a flow pack 223 in contact with the container 20.
- the upper outer case 222 may be formed using a heat insulating material having a low thermal conductivity to maintain the appearance of the flow pack 223 inserted therein.
- the fluid pack 223 is filled with a fluid 30 capable of storing heat.
- the fluid 30 may use a liquid having a high specific gravity, and generally may be filled with water that can be easily obtained and used.
- the flow pack 223 may be formed using a rubber, silicone, synthetic resin, etc., which is elastically deformable, and the outer surface of the flow pack 223 is deformed so as to be in close contact with the outer surface of the container 20.
- a portion 231 of the receiver 230 may be formed.
- the lower body portion 225 is formed of a metal mold having excellent thermal conductivity, the lower receiving groove 232 is formed in the lower portion of each container 20 in contact with the inner surface.
- the lower body portion 225 includes a lower outer case 226, a lower intermediate mold 227, and a lower inner mold 228.
- the lower outer case 226 is formed using a material such as synthetic resin and rubber having low thermal conductivity, so that the heat of the heating element 260 that is disposed inside the lower outer case 226 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 20 at warm and constant temperature.
- the lower intermediate mold 227 is formed to be inserted into the lower outer case 226, and a space in which the heating element 260 is disposed is provided.
- the lower inner mold 228 is inserted into an arrangement space provided in the lower intermediate mold 227, and lower receiving grooves 232 are formed at the upper portion of each container 20 to contact the inner surface thereof.
- the lower inner mold 228 may be used by replacing the lower receiving groove 232 is formed to match the outer shape of the container 20 that can be provided in various forms.
- the lower intermediate mold 227 and the lower inner mold 228 are formed of a metal mold having excellent thermal conductivity, and the heat generated from the heating element 260 is easily conducted, and the container is inserted into the lower receiving groove 232. 20) can conduct heat evenly.
- the container 20 is disposed in the lower accommodating groove 232 formed in the lower body portion 225 while the upper body portion 221 is opened to open the front surface of the lower body portion 225, and the upper body portion 221 is disposed.
- the container 20 may be disposed in the container accommodating part 230 by closing the front surface of the lower body part 225.
- the flow pack 223 of the upper body portion 221 is pressed by the remaining portion that can not be accommodated in the lower receiving groove 232 of the vessel 20 part 231 of the shape changed temporarily of the upper receiving groove It will play a role. Therefore, when the upper body portion 221 and the lower body portion 225 is closed, the lower receiving groove formed in the portion 231 and the lower body portion 225 pressed by the upper body portion 221 by the container 20 ( 232 forms one vessel receiving portion 230.
- the upper body portion 221 and the lower body portion 225 are foldably coupled so that the upper body portion 221 to expose the front of the lower body portion 225 lower body portion 225
- the upper body portion 221 and the lower The body parts 225 may be prevented from being spaced apart from each other.
- the upper body portion 221 and the lower body portion 225 may be provided separately, in the case of shaking the container housing body portion 220 using the shaking motion part 150, the upper body The upper body portion 221 and the lower body portion 225 may be prevented from being separated from each other by using a fixing member having a band shape to prevent the portion 221 and the lower body portion 225 from being spaced apart from each other.
- the heating element 260 may be disposed in the upper body 221 and in the lower body 225.
- the heating element 260 may be formed on the lower body portion 225, which is easy to supply power.
- the upper body portion 221 according to the present embodiment is formed by using a flexible material, when shaking the container housing body portion 220, it can effectively prevent the breakage of the container 20.
- the upper body portion 221 is different from the constant temperature stirring device 100 according to the first embodiment of replacing both the upper inner mold 124 and the lower inner mold 129 according to the shape of the container 10. Since it changes elastically with respect to the outer shape of the container 20, it is more convenient to apply a variety of containers 20 by replacing only the lower inner mold 228.
- the container housing body 220 may agitate the container 20 by using the shaking motion unit 150 described in the first embodiment, and in some cases, a separate shaker ( 5) may be used to agitate the receiving material in the vessel.
- the constant temperature stirring device according to the third embodiment of the present invention is substantially the same as the constant temperature stirring device described in the first embodiment. Therefore, the description of the constant temperature stirring apparatus in the third embodiment may refer to the description and the drawings of the constant temperature stirring apparatus of the first embodiment, and repeated content may be omitted.
- the shaking motion portion and the base of the constant temperature stirring device of the present embodiment may be used substantially the same as the shaking motion portion described in the first embodiment, the contents of the shaking motion portion and the base of the present embodiment described in the first embodiment and See description.
- Figure 5 is a perspective view of the container housing body portion of the constant temperature stirring device according to a third embodiment of the present invention
- Figure 6 is a cross-sectional view showing the container housing body portion by cutting Figure 5 in the C-C direction.
- the container 10 is provided in this embodiment in the form of a bag of the same flexible material as the first embodiment to accommodate the receiving material.
- the container accommodating body part 320 is positioned above the base 110, and includes an upper body part 321 and a lower body part 325 that cover and accommodate the upper and lower parts of the container 10, and contact the container 10.
- the inner surfaces of the upper body portion 321 and the lower body portion 325 form a container receiving portion 330 corresponding to the outer surface of the container 10. Therefore, the container 10 may receive heat through the inner surface of the container receiving portion 330.
- the upper body portion 321 of the present embodiment is empty inside, a plurality of contact contact pieces 323 in contact with the container 10 is disposed therein.
- the contact contact piece 323 is elastically supported by a plurality of coil springs 324 disposed between the inner surface of the upper body portion 321 and each contact contact piece 323.
- the contact contact piece 323 is supported so as to be displaceable with respect to the upper body portion 321, the contact contact piece 323 is displaced to be in close contact with the outer surface of the container 10, a part of the container receiving portion 330 To form.
- a part of the container accommodating part 330 may correspond to the upper accommodating groove of the first and second embodiments.
- the lower body portion 325 is formed of a metal mold having excellent thermal conductivity
- the lower receiving groove 332 is formed in the lower surface of each container 10 in contact with the inner surface. More specifically, the lower body portion 325 includes a lower outer case 326, a lower intermediate mold 327, and a lower inner mold 328.
- the lower outer case 326 is formed using a material such as synthetic resin and rubber having low thermal conductivity, so that the heat of the heating element 360 that is disposed inside the lower outer case 326 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 10 at warm and constant temperature.
- the lower intermediate mold 327 is formed to be inserted into the lower outer case 326 and has a space in which the heating element 360 is disposed.
- the lower inner mold 328 is inserted into an arrangement space provided in the lower intermediate mold 327, and lower receiving grooves 332 are formed at the upper portion of each container 10 to contact the inner surface thereof.
- the lower inner mold 328 may be used to replace the lower receiving groove 332 is formed to match the outer shape of the container 10 that can be provided in various forms.
- the lower intermediate mold 327 and the lower inner mold 328 are formed of a metal mold having excellent thermal conductivity, and the heat generated from the heating element 360 is easily conducted, and the container is inserted into the lower receiving groove 332. 10) can conduct heat evenly.
- the container 30 is disposed in the lower accommodating groove 332 formed in the lower body 325 while the upper body 321 is opened to open the front surface of the lower body 325, and the upper body 321 is disposed.
- the container 10 may be disposed in the container accommodating part 330 by closing the front surface of the lower body part 325.
- the plurality of contact contact pieces 323 disposed inside the upper body portion 321 are pressed by the remaining portion of the container 10 that is not accommodated in the lower receiving groove 332 is changed in shape.
- the container 10 is disposed in the lower receiving groove 332 formed in the lower body portion 325 in a state in which the upper body portion 321 is opened to open the front surface of the lower body portion 325, and the upper body portion ( The container 10 may be disposed in the container accommodating part 330 by closing the front surface of the lower body part 325 with 321.
- the shaking motion part 150 may shake the container housing body part 320 with respect to the base 110, and the shaking motion may include both up, down, left, and right motions or vibration motions. Therefore, the upper body portion 321 and the lower body portion 325 during the shaking motion can be combined using a fixing member 340 provided as a clip to prevent the mutual separation.
- the appropriate temperature of the container 10 while performing a shaking motion so that the mixing between the target material and the reactant contained in the container 10 is well made in this embodiment the temperature of the water to the optimum temperature of the target enzyme reaction 37 °C It can be raised to maintain its temperature for a period of time.
- the heating element 360 may be disposed in the container housing body 320 to heat the container 10, and in this embodiment, it may be preferable to arrange the inside of the lower body part 325 for convenience of installation.
- the container housing body 320 may agitate the container 20 by using the shaking motion unit 150 described in the first embodiment, and in some cases, a separate shaker is used.
- the agitation material may be stirred in the container.
- the stirring method using the constant temperature stirrer and the constant temperature stirrer according to the present invention can efficiently stir the receiving material contained in the container.
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Abstract
A constant temperature agitating device for agitating substances received in a container comprises a base, a container receiving body part and a shaking motion part. The container receiving body part is positioned at an upper part of the base, and includes upper and lower body parts for covering the upper and lower parts of the container so as to receive the container, wherein the inner surfaces of the upper and lower body parts which contact with the container form a container receiving part corresponding to the outer surface of the container. The shaking motion part shakes the container receiving body part against the base. The container receives heat via the inner surface contacting with the container receiving part.
Description
본 발명은 항온 교반장치 및 항온 교반장치를 이용한 교반방법에 관한 것으로서, 보다 자세하게는, 용기에 수용된 수용물질을 효율적으로 교반하기 위한 항온 교반장치 및 항온 교반장치를 이용한 교반방법에 관한 것이다.The present invention relates to a stirring method using a constant temperature stirrer and a constant temperature stirrer, and more particularly, to a stirring method using a constant temperature stirrer and a constant temperature stirrer for efficiently stirring the water contained in the container.
동물이나 사람의 신체로부터 채취한 시료에 효소나 화학물질을 첨가하여, 채취한 시료로부터 원하는 물질을 추출하거나 효소나 화학물질에 대한 시료의 반응을 관찰할 수 있다.Enzymes or chemicals can be added to a sample taken from an animal or human body to extract a desired substance from the sample taken or to observe the reaction of the sample to enzymes or chemicals.
이하 채취한 시료는 대상물질로 지칭될 수 있으며, 대상물질과 반응하는 효소나 화학물질은 반응물질로 지칭하고, 대상물질 및 반응물질을 모두 포괄하여 수용물질로 지칭할 수 있다.The sample collected below may be referred to as a target substance, and enzymes or chemicals that react with the target substance may be referred to as reactants, and both the target substance and the reactants may be referred to as water-soluble substances.
시료로부터 효소나 화학물질에 대한 생화학적 및 화학적 반응을 관찰할 경우에 있어서, 반응이 진행되는 동안 수용물질을 외부와 격리하기 위하여 다양한 형태로 제공되는 용기에 담아 일정시간 경과를 지켜볼 수 있다.When observing biochemical and chemical reactions of enzymes or chemicals from a sample, it is possible to observe the passage of time in containers provided in various forms in order to isolate the receiving material from the outside during the reaction.
용기는 수용물질을 수용하는 유연한 재질의 백(bag) 형태로 제공될 수도 있으며, 일정한 형태를 가지는 시험관(test tube)이나 페트리 접시(Petri-dish) 등으로 제공될 수도 있다. The container may be provided in the form of a bag made of a flexible material for accommodating the receiving material, or may be provided in a test tube or a petri dish having a certain shape.
한편, 시료가 효소나 화학물질에 대한 반응이 효과적으로 이루어지도록 하기 위하여, 용기에 수용된 수용물질을 가열하거나, 소정의 온도로 맞춘 후에 그 온도를 일정 시간 동안 유지할 수 있다. 마찬가지로, 효과적인 반응을 유도하도록 대상물질과 반응물질이 잘 혼합되도록 용기를 상하좌우로 흔들어 주거나 혹은 진동을 가할 수 있다. On the other hand, in order for the sample to react effectively with enzymes or chemicals, the water contained in the container may be heated or adjusted to a predetermined temperature and then maintained at that temperature for a predetermined time. Likewise, the container may be shaken up, down, left, or right, or vibrated to mix the target and reactant well to induce an effective reaction.
수용물질에 대한 가온 및 항온 과정은 다양한 방법을 통해서 이루어질 수 있으며, 일 예를 들면, 수용물질을 담고 있는 용기 자체를 공기를 통한 열전달 방식을 이용하는 항온기를 통해서 가온하거나 항온을 유지할 수 있다. 다른 예를 들면, 수용물질을 담고 있는 용기 자체를 물을 통한 열전달 방식을 이용하는 항온수조를 통해서 가온하거나 항온을 유지할 수 있다. The warming and constant temperature process for the receiving material may be accomplished through various methods. For example, the container containing the receiving material may be warmed or maintained at a constant temperature using a heat transfer method through air. In another example, the vessel itself containing the receiving material may be warmed or maintained at a constant temperature bath using heat transfer through water.
다만, 상술한 바와 같이 공기나 중탕을 이용한 열전달 방식은 적잖은 문제점이 있다. However, as described above, the heat transfer method using air or hot water has a considerable problem.
예를 들어, 항온기를 사용하여 공기를 통한 열전달 방법은 소정의 온도로 용기를 가열하는데 걸리는 시간이 비교적 오래 걸리며, 데워진 순환공기를 이용하여 수용물질이 목표 효소반응 최적온도인 37℃까지 도달하는 시간 많이 소요 되기 때문에 시료의 손상을 예방할 수 있으며, 목적한 물질의 제조가 용이하기 않을 수 있다. For example, the heat transfer method through the air using a thermostat takes a relatively long time to heat the vessel to a predetermined temperature, the time that the receiving material reaches the target enzyme reaction temperature of 37 ℃ using the warmed circulating air Due to the large amount required, damage to the sample can be prevented, and the preparation of the desired material may not be easy.
또한, 항온수조를 사용하여 물을 통한 열전달 방법은 상술한 공기를 통한 열전달에 비해서 비교적 빠르게 용기를 가열할 수 있으나, 용기가 물에 직접 닿아 물과 용기의 직접적인 접촉으로 가온 및 항온 과정 중 세균 및 바이러스와 같은 외부인자에 노출이 쉽게 일어날 수 있다. In addition, the heat transfer method through the water using a constant temperature water tank can heat the vessel relatively faster than the heat transfer through the air described above, but the container is in direct contact with water and the bacteria and Exposure to external factors such as viruses can easily occur.
따라서, 종래와 같은 항온기나 항온수조를 이용한 용기의 가열, 항온 유지는 상술한 이유들로 인하여 효율적이지 못하다.Therefore, the conventional heating and constant temperature maintenance of the container using a thermostat or a constant temperature bath is not efficient for the reasons mentioned above.
본 발명은 용기에 담긴 수용물질을 소정의 온도까지 비교적 빠른 시간 안에 가열하고 항온을 유지할 수 있는 항온 교반장치 및 항온 교반장치를 이용한 교반방법을 제공한다. The present invention provides a stirring method using a constant temperature stirrer and a constant temperature stirrer capable of heating the water contained in the container to a predetermined temperature in a relatively fast time and maintaining a constant temperature.
본 발명은 용기에 담긴 수용물질을 수조 내에 존재하는 오염인자들과 격리하고, 빠른 시간 내에 가열하고 항온을 유지할 수 있는 항온 교반장치 및 항온 교반장치를 이용한 교반방법을 제공한다.The present invention provides a stirring method using a constant temperature stirrer and a constant temperature stirrer capable of isolating the water contained in the container from the contaminants present in the water tank, heating and maintaining a constant temperature in a short time.
상술한 본 발명의 목적들을 달성하기 위한 본 발명의 예시적인 일 실시예에 따르면, 용기에 수용된 수용물질을 교반하기 위한 항온 교반장치는, 베이스, 베이스 상부에 위치하며, 용기의 상하부를 덮어 수용하는 상부 몸체부 및 하부 몸체부를 포함하고, 용기와 접하는 상부 몸체부 및 하부 몸체부의 내면은 용기의 외면에 대응하는 용기 수용부를 형성하는 용기수용 몸체부, 및 베이스에 대해서 용기수용 몸체부를 진탕(shaking) 운동 시키는 진탕 운동부를 구비하며, 용기와 접하는 용기 수용부의 내면을 통해서 용기로 열을 전달한다. According to an exemplary embodiment of the present invention for achieving the above object of the present invention, the constant temperature stirring device for stirring the receiving material contained in the container, is located on the base, the upper part of the base to cover the upper and lower parts of the container An inner surface of the upper body portion and the lower body portion, the upper body portion and the lower body portion in contact with the container, the container receiving body portion forming a container receiving portion corresponding to the outer surface of the container, and shaking the container receiving body portion relative to the base It is provided with a shaking motion to exercise, the heat transfer to the container through the inner surface of the container receiving portion in contact with the container.
용기 수용부의 내면을 통해서 용기로 열을 전달하는 방법을 이용한 용기의 가온 및 항온 유지를 구현하여, 종래의 항온기나 항온수조를 통해서 열을 전달하는 방법과 비교할 때, 항온수조를 통해서 열을 직접 전달하는 것과 유사한 열전달 효율을 구현하고, 물을 매개로 열전달이 이루어지는 것이 아니기 때문에 세균이나 바이러스와 같은 외부 오염인자에 대한 노출이 적다.By implementing the heating and constant temperature of the container using the method of transferring heat to the container through the inner surface of the container receiving portion, compared to the method of transferring heat through a conventional thermostat or a constant temperature bath, heat is transferred directly through the constant temperature water tank. Since heat transfer efficiency is similar to that of water, and heat transfer is not performed through water, there is little exposure to external contaminants such as bacteria and viruses.
용기 수용부는 다양한 방법을 통해서 형성할 수 있으며, 일 예로, 상부 몸체부 및 하부 몸체부 중 적어도 하나는, 용기과 접하는 고체 금형을 포함하며, 고체 금형은 용기의 외면에 밀착할 수 있도록 대응하여 형성된 수용홈을 포함하고, 수용홈이 용기 수용부의 일부를 형성할 수 있다. The container receiving part may be formed through various methods. For example, at least one of the upper body part and the lower body part may include a solid mold in contact with the container, and the solid mold may be formed to correspond to the outer surface of the container. It includes a groove, the receiving groove may form part of the container receiving portion.
또한, 다른 예로, 상부 몸체부 및 하부 몸체부 중 적어도 하나는, 용기와 접하는 유동 팩을 포함하며, 유동 팩의 내부에는 열을 저장할 수 있는 유체가 충진되며, 유동 팩의 외면은 용기의 외면에 밀착할 수 있도록 변형되어 용기 수용부의 일부를 형성할 수 있다. 이때, 유동 팩은 탄성 변형이 가능한 재질로 형성될 수 있다. In another example, at least one of the upper body portion and the lower body portion includes a flow pack in contact with the container, the inside of the flow pack is filled with a fluid capable of storing heat, the outer surface of the flow pack to the outer surface of the container It may be deformed to be in close contact to form part of the container receiving portion. At this time, the flow pack may be formed of a material capable of elastic deformation.
또한, 또 다른 예로, 상부 몸체부 및 하부 몸체부 중 적어도 하나는, 용기과 접하는 복수개의 접촉 밀착편을 포함하며, 접촉 밀착편은 상부 몸체부 또는 하부 몸체부에 대해 변위 가능하게 지지되고, 접촉 밀착편은 용기의 외면에 밀착할 수 있도록 변위되면서 용기 수용부의 일부를 형성할 수 있다. In still another example, at least one of the upper body portion and the lower body portion includes a plurality of contact adhesive pieces contacting the container, and the contact adhesive pieces are supported to be displaceable with respect to the upper body portion or the lower body portion, and the contact closely adheres to each other. The piece may be displaced to be in close contact with the outer surface of the container and form part of the container receiving portion.
물론, 상부 몸체부 및 하부 몸체부 중 적어도 하나는 발열체를 포함하여, 발열체로부터 발생한 열을 용기로 전달할 수 있으며, 발열체는 용기 수용부에 인접하여 배치되는 것이 바람직하다. Of course, at least one of the upper body portion and the lower body portion includes a heating element, it is possible to transfer the heat generated from the heating element to the container, the heating element is preferably disposed adjacent to the container receiving portion.
또한, 본 발명의 예시적인 다른 실시예에 따르면, 진탕 운동이 가능한 진탕운동 베이스 상에 결합되어 용기에 수용된 수용물질을 교반하기 위한 교반장치용 용기수용 몸체부는, 용기의 상하부를 덮어 수용하는 상부 몸체부 및 하부 몸체부를 포함하고, 용기와 접하는 상부 몸체부 및 하부 몸체부의 내면은 용기의 외면에 대응하는 용기 수용부를 형성하고, 용기와 접하는 용기 수용부의 내면을 통해서 상기 용기로 열을 전달할 수 있다. Further, according to another exemplary embodiment of the present invention, the container receiving body portion for the stirring device for agitating the receiving material contained in the container coupled to the shaking motion base capable of shaking motion, the upper body to accommodate the upper and lower parts of the container An inner surface of the upper body portion and the lower body portion, which includes a portion and a lower body portion, and forms a container receiving portion corresponding to the outer surface of the container, and may transfer heat to the container through the inner surface of the container receiving portion in contact with the container.
본 발명에 따른 용기수용 몸체부는 항온 교반장치의 베이스 혹은 진탕 운동부와 분리 가능하게 결합될 수 있으며, 다양한 형태로 제공되는 용기에 맞춰 용기수용 몸체부를 교체하여 사용할 수 있다. 참고로, 베이스는 진탕 운동부와 별도로 제공될 수도 있으며, 진탕 운동부의 상면을 지칭할 수도 있다. Container housing body portion according to the present invention can be detachably combined with the base or shaking motion portion of the constant temperature stirring device, can be used to replace the container housing body portion to match the container provided in various forms. For reference, the base may be provided separately from the shaking motion part, and may refer to the upper surface of the shaking motion part.
또한, 본 발명의 예시적인 또 다른 실시예에서는, 교반장치를 이용하여 용기에 수용된 수용물질을 교반하기 위한 교반방법이 개시된다. 교반방법은, 용기의 상하부를 덮어 수용하는 상부 몸체부 및 하부 몸체부를 포함하고, 용기와 접하는 상부 몸체부 및 하부 몸체부의 내면은 용기의 외면에 대응하는 용기 수용부를 형성하는 용기수용 몸체부를 구비하는 교반장치를 제공하는 단계, 상부 몸체부 및 하부 몸체부 사이에 용기를 위치시키는 단계, 상부 몸체부 및 하부 몸체부의 대향하는 내면을 상기 용기의 외면에 밀착시키는 단계, 용기수용 몸체부를 진탕 운동 시키는 단계, 및 용기와 접하는 상기 용기 수용부의 내면을 통해서 용기를 특정 온도로 가온하는 단계를 구비한다.In still another exemplary embodiment of the present invention, a stirring method for agitating a receiving material contained in a container using a stirring device is disclosed. The stirring method includes an upper body portion and a lower body portion covering the upper and lower portions of the container, and inner surfaces of the upper body portion and the lower body portion in contact with the container include a container accommodating body portion forming a container accommodating portion corresponding to the outer surface of the container. Providing a stirring device, positioning a container between the upper body portion and the lower body portion, bringing the opposite inner surface of the upper body portion and the lower body portion into close contact with the outer surface of the vessel, and shaking the container holding body portion. And warming the vessel to a specific temperature through the inner surface of the vessel receiving portion in contact with the vessel.
본 발명의 항온 교반장치 및 교반방법은 용기에 담긴 수용물질을 원하는 온도까지 비교적 빠른 시간 안에 가열하여 시료의 손상을 최소화하고, 목적한 물질의 제조가 효율적으로 이루어 질 수 있다.The constant temperature stirring device and the stirring method of the present invention can minimize the damage to the sample by heating the water contained in the container to a desired temperature in a relatively fast time, the production of the desired material can be made efficiently.
본 발명의 항온 교반장치 및 교반방법은 수조 내에 존재하는 오염인자들과 격리하고, 빠른 시간 내에 가열하고 항온을 유지할 수 있는 동시에 수용물질을 교반할 수 있다.The constant temperature stirrer and the stirring method of the present invention can be isolated from the contaminants present in the water bath, can be heated and maintained at a constant time, and at the same time can agitate the receiving material.
도 1은 본 발명의 제1 실시예에 따른 항온 교반장치를 도시한 사시도이다. 1 is a perspective view showing a constant temperature stirring device according to a first embodiment of the present invention.
도 2는 도 1의 항온 교반장치를 A-A방향으로 절개하여 용기수용 몸체부를 부분적으로 도시한 단면도이다. 2 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of FIG. 1 in the A-A direction.
도 3은 본 발명의 제2 실시예에 따른 항온 교반장치를 도시한 부분 사시도이다. Figure 3 is a partial perspective view showing a constant temperature stirring device according to a second embodiment of the present invention.
도 4는 도 3의 항온 교반장치를 B-B방향으로 절개하여 용기수용 몸체부를 부분적으로 도시한 단면도이다. 4 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of FIG. 3 in the B-B direction.
도 5는 본 발명의 제3 실시예에 따른 항온 교반장치 중 용기수용 몸체부의 사시도이다. Figure 5 is a perspective view of the container housing body portion of the constant temperature stirring device according to a third embodiment of the present invention.
도 6은 도 5를 C-C방향으로 절개하여 용기수용 몸체부를 도시한 단면도이다.FIG. 6 is a cross-sectional view illustrating the container housing body part by cutting FIG. 5 in the C-C direction.
이하 첨부된 도면들을 참조하여 본 발명의 바람직한 실시예를 상세하게 설명하지만, 본 발명이 실시예에 의해 제한되거나 한정되는 것은 아니다. 참고로, 본 설명에서 동일한 번호는 실질적으로 동일한 요소를 지칭하며, 이러한 규칙 하에서 다른 도면에 기재된 내용을 인용하여 설명할 수 있고, 당업자에게 자명하다고 판단되거나 반복되는 내용은 생략될 수 있다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited or limited by the embodiments. For reference, in the present description, the same numbers refer to substantially the same elements, and may be described by referring to the contents described in the other drawings under these rules, and the contents determined to be obvious to those skilled in the art or repeated may be omitted.
실시예1Example 1
도 1은 본 발명의 제1 실시예에 따른 항온 교반장치를 도시한 사시도이며, 도 2는 도 1의 항온 교반장치를 A-A방향으로 절개하여 용기수용 몸체부를 부분적으로 도시한 단면도이다. 1 is a perspective view showing a constant temperature stirring device according to a first embodiment of the present invention, Figure 2 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of Figure 1 in the A-A direction.
도 1 및 도 2를 참조하면, 용기(10)에 수용된 수용물질을 교반하기 위한 항온 교반장치(100)는 베이스(110), 용기수용 몸체부(120) 및 진탕 운동부(150)를 구비한다.1 and 2, the constant temperature agitation device 100 for agitating the receiving material contained in the container 10 includes a base 110, a container housing body 120, and a shaking motion part 150.
지방조직의 줄기세포는 인체 내 손상된 각종 조직의 재생에 쓰이기 위한 목적으로 사용될 수 있으며, 특히 조혈세포, 신경세포, 피부재건, 연골세포, 심근세포, 혈관세포 등으로 분화 가능하기 때문에 다양한 분야에서의 사용이 가능하다. 한편, 골수, 제대혈, 말초혈액, 배아 등에서 줄기세포를 분리할 경우에는 윤리문제, 채취 대상자의 통증문제, 처리과정 중의 세균, 바이러스 등 외부인자에 의한 오염과 같이 복잡한 문제점을 극복해야 하기 때문에 최근 지방줄기세포의 유용성은 더욱 부각되고 있다. Stem cells of adipose tissue can be used for the purpose of regenerating various damaged tissues in the human body, and in particular, they can be differentiated into hematopoietic cells, nerve cells, skin reconstructions, chondrocytes, cardiomyocytes, and blood vessel cells. Can be used. On the other hand, when stem cells are separated from bone marrow, umbilical cord blood, peripheral blood, and embryos, it is necessary to overcome complex problems such as ethical issues, pain of the subjects to be collected, and contamination by external factors such as bacteria and viruses during the process. The usefulness of stem cells is becoming more important.
예를 들면 본 실시예에 따른 항온 교반장치를 사용하여 인체 내의 잉여 지방조직을 시료로 사용하고, 이를 이용하여 상술한 지방줄기세포를 분리할 수 있다. For example, using a constant temperature agitation device according to the present embodiment using the excess adipose tissue in the human body as a sample, it can be used to isolate the adipose stem cells described above.
이하 본 명세서에서 채취한 시료는 대상물질로 지칭하며, 대상물질과 반응하는 효소나 화학물질은 반응물질로 지칭하고, 대상물질 및 반응물질을 모두 포괄하여 수용물질로 지칭할 수 있다. Hereinafter, the sample collected in the present specification may be referred to as a target substance, enzymes or chemicals reacting with the target substance may be referred to as a reactant, and both the target substance and the reactant may be referred to as water-soluble substances.
용기(10)는 본 실시예에서는 수용물질을 수용하는 유연한 재질의 백(bag) 형태로 제공된다. The container 10 is provided in this embodiment in the form of a bag made of a flexible material to accommodate the receiving material.
베이스(110)는 판상으로 제공되며, 베이스(110)의 저면은 진탕 운동부(150) 내부에 배치된 모터의 회전운동을 상하좌우 및 진동운동으로 변형하는 기어 조립체의 출력축(151)과 연결된다. The base 110 is provided in a plate shape, the bottom surface of the base 110 is connected to the output shaft 151 of the gear assembly for transforming the rotational movement of the motor disposed in the shaking motion unit 150 to the up, down, left and right and vibration movement.
또한, 베이스(110)는 용기수용 몸체부(120) 중 하부 몸체부(125)와 결합된다. 구체적으로 베이스(110)의 나란한 모서리로부터 상부를 향하여 연장된 한 쌍의 훅크부(111)가 형성되며, 하부 몸체부(125)의 양 측면에는 훅크부(111)가 삽입되는 한 쌍의 결합 홈(129)이 형성된다. 이에 베이스(110)로부터 용기수용 몸체부(120)가 분리 가능하게 결합될 수 있다. In addition, the base 110 is coupled to the lower body portion 125 of the container receiving body portion 120. Specifically, a pair of hook portions 111 extending upward from the side edges of the base 110 are formed, and a pair of coupling grooves in which the hook portions 111 are inserted into both side surfaces of the lower body portion 125 is formed. 129 is formed. The container housing body 120 may be detachably coupled to the base 110.
용기수용 몸체부(120)는 베이스(110) 상부에 위치하며, 용기(10)의 상하부를 덮어 수용하는 상부 몸체부(121) 및 하부 몸체부(125)를 포함하고, 용기(10)와 접하는 상부 몸체부(121) 및 하부 몸체부(125)의 내면은 용기(10)의 외면에 대응하는 용기 수용부(130)를 형성하고, 용기(10)는 용기 수용부(130)와 접하는 내면을 통해서 열을 전달받을 수 있다. The container accommodating body part 120 is positioned above the base 110 and includes an upper body part 121 and a lower body part 125 that cover and accommodate the upper and lower parts of the container 10, and contact the container 10. The inner surfaces of the upper body portion 121 and the lower body portion 125 form a container receiving portion 130 corresponding to the outer surface of the container 10, the container 10 is an inner surface in contact with the container receiving portion 130 Heat can be transmitted through.
구체적으로, 상부 몸체부(121)는 상부 외부케이스(122), 상부 중간금형(123), 및 상부 내부금형(124)을 포함한다. Specifically, the upper body portion 121 includes an upper outer case 122, an upper intermediate mold 123, and an upper inner mold 124.
상부 외부케이스(122)는 열전도성이 작은 합성수지 및 고무와 같은 재질을 이용하여 형성하여, 상부 외부케이스(122)의 내부에 배치되어 열을 발생하는 발열체(160)의 열이 외부로 전도되지 않도록 하여 빠르고 경제적으로 용기(10)를 가온 및 항온으로 유지할 수 있다. The upper outer case 122 is formed using a material such as synthetic resin and rubber having low thermal conductivity, so that the heat of the heating element 160 disposed inside the upper outer case 122 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 10 at warm and constant temperature.
상부 중간금형(123)은 상부 외부케이스(122)의 내부에 삽입되도록 형성되며, 발열체(160)가 배치되는 공간이 마련된다. The upper intermediate mold 123 is formed to be inserted into the upper outer case 122, and a space in which the heating element 160 is disposed is provided.
상부 내부금형(124)은 상부 중간금형(123)에 마련된 배치 공간에 삽입되며, 각각의 용기(10)의 상부에서 그 내면이 접하는 상부 수용홈(131)들이 형성되어 있다. 참고로, 상부 내부금형(124)은 다양한 형태로 제공될 수 있는 용기(10)의 외형에 맞는 상부 수용홈(131)이 형성되어 있는 것으로 교체하여 사용할 수 있다. The upper inner mold 124 is inserted into the arrangement space provided in the upper intermediate mold 123, the upper receiving grooves 131 are formed in the upper surface of each container 10 in contact with the inner surface. For reference, the upper inner mold 124 may be used to replace the upper receiving groove 131 is formed to match the outer shape of the container 10 that can be provided in various forms.
상부 중간금형(123) 및 상부 내부금형(124)은 열전도성이 뛰어난 금속 금형으로 형성되어 있으며, 발열체(160)로부터 발생된 열이 쉽게 전도되고, 이에 상부 수용홈(131)에 삽입되는 용기(10)에 열을 골고루 전도할 수 있다. The upper middle mold 123 and the upper inner mold 124 are formed of a metal mold having excellent thermal conductivity, and the heat generated from the heating element 160 is easily conducted, and the container is inserted into the upper receiving groove 131 ( 10) can conduct heat evenly.
마찬가지로, 하부 몸체부(125)는 하부 외부케이스(126), 하부 중간금형(127), 및 하부 내부금형(128)을 포함한다. Similarly, the lower body portion 125 includes a lower outer case 126, a lower intermediate mold 127, and a lower inner mold 128.
하부 외부케이스(126)는 열전도성이 작은 합성수지 및 고무와 같은 재질을 이용하여 형성하여, 하부 외부케이스(126)의 내부에 배치되어 열을 발생하는 발열체(160)의 열이 외부로 전도되지 않도록 하여 빠르고 경제적으로 용기(10)를 가온 및 항온으로 유지할 수 있다. The lower outer case 126 is formed using a material such as synthetic resin and rubber having a low thermal conductivity, so that the heat of the heating element 160 that is disposed inside the lower outer case 126 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 10 at warm and constant temperature.
하부 중간금형(127)은 하부 외부케이스(126)의 내부에 삽입되도록 형성되며, 발열체(160)가 배치되는 공간이 마련된다. The lower intermediate mold 127 is formed to be inserted into the lower outer case 126, and a space in which the heating element 160 is disposed is provided.
하부 내부금형(128)은 하부 중간금형(127)에 마련된 배치 공간에 삽입되며, 각각의 용기(10)의 상부에서 그 내면이 접하는 하부 수용홈(132)들이 형성되어 있다. 참고로, 하부 내부금형(128)은 다양한 형태로 제공될 수 있는 용기(10)의 외형에 맞는 하부 수용홈(132)이 형성되어 있는 것으로 교체하여 사용할 수 있다. The lower inner mold 128 is inserted into an arrangement space provided in the lower intermediate mold 127, and lower receiving grooves 132 are formed at upper portions of the respective containers 10 to contact their inner surfaces. For reference, the lower inner mold 128 may be used to replace the lower receiving groove 132 is formed to match the outer shape of the container 10 that can be provided in various forms.
하부 중간금형(127) 및 하부 내부금형(128)은 열전도성이 뛰어난 금속 금형으로 형성되어 있으며, 발열체(160)로부터 발생된 열이 쉽게 전도되고, 이에 하부 수용홈(132)에 삽입되는 용기(10)에 열을 골고루 전도할 수 있다. The lower middle mold 127 and the lower inner mold 128 are formed of a metal mold having excellent thermal conductivity, and heat generated from the heating element 160 is easily conducted, and the container is inserted into the lower receiving groove 132. 10) can conduct heat evenly.
참고로, 하부 외부케이스(126)에는 후술할 진탕 운동부(150)의 외측에 제공되는 온도창과는 별개로 온도를 표시할 수 있는 온도 표시창이 제공될 수 있으며, 진탕 운동부(150)나 하부 외부케이스(126)에 제공되는 온도창은 모두 용기(10)나 발열체(160) 주위로 배치되어 그 온도를 측정하기 위한 온도센서를 통해서 온도를 표시할 수 있다.For reference, the lower outer case 126 may be provided with a temperature display window that can display the temperature separately from the temperature window provided on the outside of the shaking motion unit 150 to be described later, the shaking motion unit 150 or the lower outer case The temperature windows provided at 126 may all be disposed around the vessel 10 or the heating element 160 to display the temperature through a temperature sensor for measuring the temperature.
상술한 바와 같이, 서로 마주하도록 상부 몸체부(121) 및 하부 몸체부(125)에 각각에 형성되어 서로 마주하는 한 쌍의 상부 수용홈(131) 및 하부 수용홈(132)은 하나의 용기 수용부(130)를 형성한다. As described above, the pair of upper accommodating grooves 131 and the lower accommodating grooves 132 formed on the upper body 121 and the lower body 125 to face each other to face each other accommodate one container. The unit 130 is formed.
또한, 본 실시예에서 발열체(160)는 상부 중간금형(123) 및 하부 중간금형(127) 각각에 배치되어 있지만, 경우에 따라서는 어느 한쪽에만 설치하는 것도 가능할 것이다. 다만, 발열체(160)는 효과적인 열전도를 위하여 용기 수용부(130)에 인접하여 배치되는 것이 바람직할 것이다. In addition, in the present embodiment, the heating element 160 is disposed in each of the upper intermediate mold 123 and the lower intermediate mold 127, but in some cases it may be installed only on either side. However, the heating element 160 may be disposed adjacent to the container receiving portion 130 for effective heat conduction.
또한, 상부 몸체부(121) 및 하부 몸체부(125)는 접철식으로 결합되어 상부 몸체부(121)는 하부 몸체부(125)의 상면을 노출할 수 있도록 하부 몸체부(125)와 상대 이동 가능하게 결합된다. In addition, the upper body 121 and the lower body 125 is foldably coupled so that the upper body 121 is relatively movable with the lower body 125 so as to expose the upper surface of the lower body 125. To be combined.
이에, 상부 몸체부(121)를 하부 몸체부(125)의 전면을 개방하도록 연 상태에서 하부 몸체부(125)에 형성된 하부 수용홈(132)에 용기(10)를 배치하고, 상부 몸체부(121)로 하부 몸체부(125)의 전면을 가리도록 닫아, 용기 수용부(130)에 용기(10)를 배치할 수 있다. Thus, the container 10 is disposed in the lower receiving groove 132 formed in the lower body portion 125 while the upper body 121 is opened to open the front surface of the lower body portion 125, and the upper body portion ( The container 10 may be disposed in the container accommodating part 130 by closing the front surface of the lower body part 125.
그 후에, 진탕 운동부(150)는 베이스(110)에 대해서 용기수용 몸체부(120)를 진탕 운동 시킬 수 있으며, 진탕 운동은 상하좌우 운동이나 진동운동을 모두 포함할 수 있다. After that, the shaking motion unit 150 may shake the container housing body 120 with respect to the base 110, and the shaking motion may include both up, down, left, and right movements or vibration movements.
진탕 운동시에 상부 몸체부(121)와 하부 몸체부(125)가 상호 이격되는 것을 방지하도록 별도의 고정부재(140)를 사용할 수 있으며, 본 실시예에서 고정부재(140)는 클립 형태로 제공되어 상부 몸체부(121)와 하부 몸체부(125)를 상호 결합할 수 있다. When the upper body portion 121 and the lower body portion 125 at the time of shaking motion can be used to separate the fixing member 140, in this embodiment the fixing member 140 is provided in the form of a clip The upper body portion 121 and the lower body portion 125 may be coupled to each other.
한편, 용기(10) 내부에 담긴 대상물질과 반응물질간에 혼합이 잘 이루어 지도록 진탕 운동을 수행하면서 용기(10)를 적정한 온도 본 실시예에서는 수용물질이 목표 효소반응 최적온도인 37℃까지 온도를 상승시켜, 일정 시간 동안 그 온도를 유지할 수 있다. On the other hand, the appropriate temperature of the container 10 while performing a shaking motion so that the mixing between the target material and the reactant contained in the container 10 is well made in this embodiment the temperature of the water to the optimum temperature of the target enzyme reaction 37 ℃ It can be raised to maintain its temperature for a period of time.
참고로, 본 명세서에서 진탕 운동부(150)는 모터, 모터의 회전수 및 모터의 회전운동을 상하좌우 및 진동운동으로 변형하여 베이스(110)로 전달하는 기어박스 및 모터의 회전수를 제어하거나, 열선으로 제공되는 발열체(160)로 전달되는 전원을 조절하는 제어부 등을 포함할 수 있다. 참고로, 진탕 운동부(150)로부터 발열체(160)로 제공되는 전원은 출력축(151)을 통해서 전달될 수 있을 것이다. 또한, 발열체로 전달되는 전원은 발열체가 배치되는 상부 또는 하부 몸체부에 별도로 제공되는 배터리 혹은 별도의 교류전력으로부터 공급받을 수도 있다. For reference, in the present specification, the shaking motion unit 150 controls the rotation speed of the motor and the gearbox and the motor to be transmitted to the base 110 by transforming the rotational motion of the motor and the rotational movement of the motor up and down, left and right and vibration, It may include a control unit for controlling the power delivered to the heating element 160 provided as a heating wire. For reference, the power provided from the shaking motion unit 150 to the heating element 160 may be transmitted through the output shaft 151. In addition, the power delivered to the heating element may be supplied from a battery or a separate AC power provided separately in the upper or lower body portion in which the heating element is disposed.
발열체(160)로부터 직접 용기(10)로 전해지는 열은 상부 몸체부(121) 및 하부 몸체부(125)에 접하는 용기(10)의 온도를 순간적으로 목표온도 보다 고온으로 상승시킬 수도 있으나, 교반 또는 진탕 작용이 동시에 진행하기 때문에 실제 세포에 미치는 영향은 미비하여 세포의 손상 없이 신속하게 효소처리 과정을 진행할 수 있다. Heat transmitted directly from the heating element 160 to the container 10 may instantly raise the temperature of the container 10 in contact with the upper body 121 and the lower body 125 to a higher temperature than the target temperature, but with stirring. In addition, since the shaking action proceeds at the same time, the effect on the actual cells is insignificant and the enzyme treatment can proceed quickly without damaging the cells.
상술한 바와 같이, 용기 수용부(120)의 내면을 통해서 용기(10)로 열을 전달하는 방법은, 종래의 항온기나 항온수조를 통해서 열을 전달하는 것과 비교할 때, 항온수조를 통해서 열을 직접 전달하는 것과 유사한 열전달 효율을 구현하면서도, 물을 매개로 열전달이 이루어지는 것이 아니기 때문에 수조 내에 존재하는 오염인자들과 격리될 수 있다.As described above, the method of transferring heat to the container 10 through the inner surface of the container accommodating part 120, as compared with the transfer of heat through a conventional thermostat or a constant temperature bath, heat directly through the constant temperature water tank It achieves heat transfer efficiency similar to that of transfer, but can be isolated from the contaminants present in the bath because it is not water-borne.
실시예2Example 2
본 발명의 제2 실시예에 따른 항온 교반장치는 제1 실시예에서 설명한 항온 교반장치와 실질적으로 동일하다. 따라서, 제2 실시예에서I 항온 교반장치에 대한 설명은 제1 실시예의 항온 교반장치에 대한 설명 및 도면을 참조할 수 있으며, 반복되는 내용은 생략될 수 있다. 특히, 본 실시예의 항온 교반장치 중 진탕 운동부 및 베이스는 제1 실시예에서 설명한 진탕 운동부와 실질적으로 동일한 것을 사용할 수 있으며, 본 실시예의 진탕 운동부 및 베이스에 대한 내용은 제1 실시예에서 설명한 도면 및 설명을 참조할 수 있다. The constant temperature stirring device according to the second embodiment of the present invention is substantially the same as the constant temperature stirring device described in the first embodiment. Therefore, the description of the constant temperature stirring apparatus I in the second embodiment may refer to the description and drawings of the constant temperature stirring apparatus of the first embodiment, and the repeated content may be omitted. In particular, the shaking motion portion and the base of the constant temperature stirring device of the present embodiment may be used substantially the same as the shaking motion portion described in the first embodiment, the contents of the shaking motion portion and the base of the present embodiment described in the first embodiment and See description.
다만, 본 발명의 제2 실시예에서는 제1 실시예의 구성요소와 차이가 있는 용기수용 몸체부에 대해서 보다 구체적으로 설명한다. However, in the second embodiment of the present invention will be described in more detail with respect to the container housing body portion different from the components of the first embodiment.
도 3은 본 발명의 제2 실시예에 따른 항온 교반장치를 도시한 부분 사시도이며, 도 4는 도 3의 항온 교반장치를 B-B방향으로 절개하여 용기수용 몸체부를 부분적으로 도시한 단면도이다. Figure 3 is a partial perspective view showing a constant temperature stirring device according to a second embodiment of the present invention, Figure 4 is a cross-sectional view partially showing the container housing body part by cutting the constant temperature stirring device of Figure 3 in the B-B direction.
도 3 및 도 4를 참조하면, 용기(20)는 본 실시예에서는 수용물질을 수용하도록 일정한 형태를 가지는 시험관(test tube)으로 제공된다. 3 and 4, the container 20 is provided as a test tube having a certain shape to accommodate the receiving material in this embodiment.
용기수용 몸체부(220)는 베이스(110) 상부에 위치하며, 용기(20)의 상하부를 덮어 수용하는 상부 몸체부(221) 및 하부 몸체부(225)를 포함하고, 용기(20)와 접하는 상부 몸체부(221) 및 하부 몸체부(225)의 내면은 용기(20)의 외면에 대응하는 용기 수용부(230)를 형성하고, 용기(20)는 용기 수용부(230)와 접하는 내면을 통해서 열을 전달받을 수 있다. The container accommodating body part 220 is positioned above the base 110 and includes an upper body part 221 and a lower body part 225 that cover and accommodate the upper and lower parts of the container 20, and are in contact with the container 20. The inner surfaces of the upper body portion 221 and the lower body portion 225 form a container receiving portion 230 corresponding to the outer surface of the container 20, the container 20 is the inner surface in contact with the container receiving portion 230 Heat can be transmitted through.
구체적으로, 상부 몸체부(221)는 상부 외부케이스(222) 및 용기(20)와 접하는 유동 팩(223)을 포함한다. 구체적으로 설명하면, 상부 외부케이스(222)는 내부에 삽입되는 유동 팩(223)의 외형을 유지하고, 열전도성이 낮은 단열성 재질을 이용하여 형성될 수 있다. 유동 팩(223)의 내부에는 열을 저장할 수 있는 유체(30)가 충진되어 있다. 유체(30)는 열비중이 높은 액체를 사용할 수 있으며, 일반적으로 쉽게 구하여 사용할 수 있는 물을 충진하여 사용할 수 있다. 이때, 유동 팩(223)은 탄성 변형이 가능한 고무나, 실리콘 및 합성수지 등을 이용하여 형성할 수 있으며, 이에 유동 팩(223)의 외면은 용기(20)의 외면에 밀착할 수 있도록 변형되어 용기 수용부(230)의 일부(231)를 형성할 수 있다.Specifically, the upper body portion 221 includes an upper outer case 222 and a flow pack 223 in contact with the container 20. Specifically, the upper outer case 222 may be formed using a heat insulating material having a low thermal conductivity to maintain the appearance of the flow pack 223 inserted therein. The fluid pack 223 is filled with a fluid 30 capable of storing heat. The fluid 30 may use a liquid having a high specific gravity, and generally may be filled with water that can be easily obtained and used. At this time, the flow pack 223 may be formed using a rubber, silicone, synthetic resin, etc., which is elastically deformable, and the outer surface of the flow pack 223 is deformed so as to be in close contact with the outer surface of the container 20. A portion 231 of the receiver 230 may be formed.
한편, 하부 몸체부(225)는 열전도성이 뛰어난 금속 금형으로 형성되어 있으며, 각각의 용기(20)의 하부에서 그 내면이 접하는 하부 수용홈(232)이 형성되어 있다. 보다 구체적으로 설명하면, 하부 몸체부(225)는 하부 외부케이스(226), 하부 중간금형(227), 및 하부 내부금형(228)을 포함한다. On the other hand, the lower body portion 225 is formed of a metal mold having excellent thermal conductivity, the lower receiving groove 232 is formed in the lower portion of each container 20 in contact with the inner surface. In more detail, the lower body portion 225 includes a lower outer case 226, a lower intermediate mold 227, and a lower inner mold 228.
하부 외부케이스(226)는 열전도성이 작은 합성수지 및 고무와 같은 재질을 이용하여 형성하여, 하부 외부케이스(226)의 내부에 배치되어 열을 발생하는 발열체(260)의 열이 외부로 전도되지 않도록 하여 빠르고 경제적으로 용기(20)를 가온 및 항온으로 유지할 수 있다. The lower outer case 226 is formed using a material such as synthetic resin and rubber having low thermal conductivity, so that the heat of the heating element 260 that is disposed inside the lower outer case 226 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 20 at warm and constant temperature.
하부 중간금형(227)은 하부 외부케이스(226)의 내부에 삽입되도록 형성되며, 발열체(260)가 배치되는 공간이 마련된다. The lower intermediate mold 227 is formed to be inserted into the lower outer case 226, and a space in which the heating element 260 is disposed is provided.
하부 내부금형(228)은 하부 중간금형(227)에 마련된 배치 공간에 삽입되며, 각각의 용기(20)의 상부에서 그 내면이 접하는 하부 수용홈(232)들이 형성되어 있다. 참고로, 하부 내부금형(228)은 다양한 형태로 제공될 수 있는 용기(20)의 외형에 맞는 하부 수용홈(232)이 형성되어 있는 것으로 교체하여 사용할 수 있다. The lower inner mold 228 is inserted into an arrangement space provided in the lower intermediate mold 227, and lower receiving grooves 232 are formed at the upper portion of each container 20 to contact the inner surface thereof. For reference, the lower inner mold 228 may be used by replacing the lower receiving groove 232 is formed to match the outer shape of the container 20 that can be provided in various forms.
하부 중간금형(227) 및 하부 내부금형(228)은 열전도성이 뛰어난 금속 금형으로 형성되어 있으며, 발열체(260)로부터 발생된 열이 쉽게 전도되고, 이에 하부 수용홈(232)에 삽입되는 용기(20)에 열을 골고루 전도할 수 있다. The lower intermediate mold 227 and the lower inner mold 228 are formed of a metal mold having excellent thermal conductivity, and the heat generated from the heating element 260 is easily conducted, and the container is inserted into the lower receiving groove 232. 20) can conduct heat evenly.
상부 몸체부(221)를 하부 몸체부(225)의 전면을 개방하도록 연 상태에서 하부 몸체부(225)에 형성된 하부 수용홈(232)에 용기(20)를 배치하고, 상부 몸체부(221)로 하부 몸체부(225)의 전면을 가리도록 닫아, 용기 수용부(230)에 용기(20)를 배치할 수 있다. The container 20 is disposed in the lower accommodating groove 232 formed in the lower body portion 225 while the upper body portion 221 is opened to open the front surface of the lower body portion 225, and the upper body portion 221 is disposed. The container 20 may be disposed in the container accommodating part 230 by closing the front surface of the lower body part 225.
이때, 상부 몸체부(221)의 유동 팩(223)은 용기(20)의 하부 수용홈(232)에서 수용하지 못한 나머지부분에 의해서 눌려 그 형태가 변한 일부(231)가 일시적으로 상부 수용홈의 역할을 하게 된다. 따라서, 상부 몸체부(221)와 하부 몸체부(225)가 닫히면, 상부 몸체부(221)가 용기(20)에 의해서 눌린 부분(231)과 하부 몸체부(225)에 각각 형성된 하부 수용홈(232)이 하나의 용기 수용부(230)를 형성한다. At this time, the flow pack 223 of the upper body portion 221 is pressed by the remaining portion that can not be accommodated in the lower receiving groove 232 of the vessel 20 part 231 of the shape changed temporarily of the upper receiving groove It will play a role. Therefore, when the upper body portion 221 and the lower body portion 225 is closed, the lower receiving groove formed in the portion 231 and the lower body portion 225 pressed by the upper body portion 221 by the container 20 ( 232 forms one vessel receiving portion 230.
또한, 본 실시예에서는 상부 몸체부(221) 및 하부 몸체부(225)는 접철식으로 결합되어 상부 몸체부(221)가 하부 몸체부(225)의 전면을 노출시킬 수 있도록 하부 몸체부(225)에 대하여 상대 이동 가능하게 결합되며, 클립 형태의 고정부재(240)를 사용하여 진탕 운동부(150)를 이용하여 용기수용 몸체부(220)를 진탕 운동 시킬 경우에, 상부 몸체부(221)와 하부 몸체부(225)가 상호 이격되는 것을 방지할 수 있다. In addition, in the present embodiment, the upper body portion 221 and the lower body portion 225 are foldably coupled so that the upper body portion 221 to expose the front of the lower body portion 225 lower body portion 225 When coupled to the relative movement with respect to the shaking motion container container body 220 using the shaking motion unit 150 using the clip-shaped fixing member 240, the upper body portion 221 and the lower The body parts 225 may be prevented from being spaced apart from each other.
경우에 따라서는, 상부 몸체부(221) 및 하부 몸체부(225)는 분리되어 제공될 수도 있으며, 진탕 운동부(150)를 이용하여 용기수용 몸체부(220)를 진탕 운동 시킬 경우에, 상부 몸체부(221)와 하부 몸체부(225)가 상호 이격되는 것을 방지하는 밴드 형태의 고정부재를 사용하여 상부 몸체부(221) 및 하부 몸체부(225)가 상호 이격되는 것을 방지할 수 있다. In some cases, the upper body portion 221 and the lower body portion 225 may be provided separately, in the case of shaking the container housing body portion 220 using the shaking motion part 150, the upper body The upper body portion 221 and the lower body portion 225 may be prevented from being separated from each other by using a fixing member having a band shape to prevent the portion 221 and the lower body portion 225 from being spaced apart from each other.
한편, 용기(20)를 가열하기 위하여, 상부 몸체부(221)의 내부 및 하부 몸체부(225)의 내부에는 발열체(260)가 배치될 수 있다. 다만, 상부 몸체부(221)는 하부 몸체부(225)와 분리되어 제공되는 관계로, 발열체(260)는 전원의 공급이 용이한 하부 몸체부(225)에 형성되는 것이 바람직할 것이다. Meanwhile, in order to heat the container 20, the heating element 260 may be disposed in the upper body 221 and in the lower body 225. However, since the upper body portion 221 is provided separately from the lower body portion 225, the heating element 260 may be formed on the lower body portion 225, which is easy to supply power.
본 실시예에 따른 상부 몸체부(221)는 유연한 재질을 이용하여 형성됨으로써, 용기수용 몸체부(220)를 진탕 운동 시킬 경우에, 용기(20)의 파손을 효과적으로 방지할 수 있다. The upper body portion 221 according to the present embodiment is formed by using a flexible material, when shaking the container housing body portion 220, it can effectively prevent the breakage of the container 20.
또한, 용기(10)의 형태에 따라서 상부 내부금형(124)과 하부 내부금형(129)을 모두 교체하는 제1 실시예에 따른 항온 교반장치(100)와는 다르게 상부 몸체부(221)의 형태가 용기(20)의 외형에 대해서 탄성적으로 변화하기 때문에, 하부 내부금형(228)만을 교체하여 다양한 용기(20)을 적용하기에 더욱 편리하다. In addition, the upper body portion 221 is different from the constant temperature stirring device 100 according to the first embodiment of replacing both the upper inner mold 124 and the lower inner mold 129 according to the shape of the container 10. Since it changes elastically with respect to the outer shape of the container 20, it is more convenient to apply a variety of containers 20 by replacing only the lower inner mold 228.
참고로, 본 실시예에 따른 용기수용 몸체부(220)는 제1 실시예에서 설명한 진탕 운동부(150)를 이용하여 용기(20)를 교반하는 것이 가능하며, 경우에 따라서는 별도의 진탕기(5)를 사용하여 용기 내에 수용물질을 교반시킬 수도 있을 것이다. For reference, the container housing body 220 according to the present embodiment may agitate the container 20 by using the shaking motion unit 150 described in the first embodiment, and in some cases, a separate shaker ( 5) may be used to agitate the receiving material in the vessel.
실시예3Example 3
본 발명의 제3 실시예에 따른 항온 교반장치는 제1 실시예에서 설명한 항온 교반장치와 실질적으로 동일하다. 따라서, 제3 실시예에서 항온 교반장치에 대한 설명은 제1 실시예의 항온 교반장치에 대한 설명 및 도면을 참조할 수 있으며, 반복되는 내용은 생략될 수 있다. 특히, 본 실시예의 항온 교반장치 중 진탕 운동부 및 베이스는 제1 실시예에서 설명한 진탕 운동부와 실질적으로 동일한 것을 사용할 수 있으며, 본 실시예의 진탕 운동부 및 베이스에 대한 내용은 제1 실시예에서 설명한 도면 및 설명을 참조할 수 있다. The constant temperature stirring device according to the third embodiment of the present invention is substantially the same as the constant temperature stirring device described in the first embodiment. Therefore, the description of the constant temperature stirring apparatus in the third embodiment may refer to the description and the drawings of the constant temperature stirring apparatus of the first embodiment, and repeated content may be omitted. In particular, the shaking motion portion and the base of the constant temperature stirring device of the present embodiment may be used substantially the same as the shaking motion portion described in the first embodiment, the contents of the shaking motion portion and the base of the present embodiment described in the first embodiment and See description.
다만, 본 발명의 제3 실시예에서는 제1 실시예의 구성요소와 차이가 있는 용기수용 몸체부에 대해서 보다 구체적으로 설명한다. However, in the third embodiment of the present invention will be described in more detail with respect to the container housing body portion different from the components of the first embodiment.
도 5는 본 발명의 제3 실시예에 따른 항온 교반장치 중 용기수용 몸체부의 사시도이며, 도 6은 도 5를 C-C방향으로 절단하여 용기수용 몸체부를 도시한 단면도이다. Figure 5 is a perspective view of the container housing body portion of the constant temperature stirring device according to a third embodiment of the present invention, Figure 6 is a cross-sectional view showing the container housing body portion by cutting Figure 5 in the C-C direction.
도 5 및 도 6을 참조하면, 용기(10)는 본 실시예에서는 수용물질을 수용하도록 제1 실시예와 동일한 유연한 재질의 백(bag) 형태로 제공된다. 5 and 6, the container 10 is provided in this embodiment in the form of a bag of the same flexible material as the first embodiment to accommodate the receiving material.
용기수용 몸체부(320)는 베이스(110) 상부에 위치하며, 용기(10)의 상하부를 덮어 수용하는 상부 몸체부(321) 및 하부 몸체부(325)를 포함하고, 용기(10)와 접하는 상부 몸체부(321) 및 하부 몸체부(325)의 내면은 용기(10)의 외면에 대응하는 용기 수용부(330)를 형성한다. 따라서, 용기(10)는 용기 수용부(330)와 접하는 내면을 통해서 열을 전달받을 수 있다.The container accommodating body part 320 is positioned above the base 110, and includes an upper body part 321 and a lower body part 325 that cover and accommodate the upper and lower parts of the container 10, and contact the container 10. The inner surfaces of the upper body portion 321 and the lower body portion 325 form a container receiving portion 330 corresponding to the outer surface of the container 10. Therefore, the container 10 may receive heat through the inner surface of the container receiving portion 330.
본 실시예의 상부 몸체부(321)는 내부가 비어 있으며, 그 내부에는 용기(10)와 접하는 복수개의 접촉 밀착편(323)이 배치된다. 접촉 밀착편(323)은 상부 몸체부(321)의 내면과 각각의 접촉 밀착편(323) 사이에 배치되는 복수개의 코일 스프링(324)에 의해서 탄성적으로 지지된다.The upper body portion 321 of the present embodiment is empty inside, a plurality of contact contact pieces 323 in contact with the container 10 is disposed therein. The contact contact piece 323 is elastically supported by a plurality of coil springs 324 disposed between the inner surface of the upper body portion 321 and each contact contact piece 323.
이에 접촉 밀착편(323)은 상부 몸체부(321)에 대해 변위 가능하게 지지되고, 접촉 밀착편(323)은 용기(10)의 외면에 밀착할 수 있도록 변위되면서 용기 수용부(330)의 일부를 형성한다. 여기서 용기 수용부(330)의 일부는 앞선 제1 및 제2 실시예의 상부 수용홈에 대응할 수 있다. The contact contact piece 323 is supported so as to be displaceable with respect to the upper body portion 321, the contact contact piece 323 is displaced to be in close contact with the outer surface of the container 10, a part of the container receiving portion 330 To form. Here, a part of the container accommodating part 330 may correspond to the upper accommodating groove of the first and second embodiments.
한편, 하부 몸체부(325)는 열전도성이 뛰어난 금속 금형으로 형성되어 있으며, 각각의 용기(10)의 하부에서 그 내면이 접하는 하부 수용홈(332)이 형성되어 있다. 보다 구체적으로 설명하면, 하부 몸체부(325)는 하부 외부케이스(326), 하부 중간금형(327), 및 하부 내부금형(328)을 포함한다. On the other hand, the lower body portion 325 is formed of a metal mold having excellent thermal conductivity, the lower receiving groove 332 is formed in the lower surface of each container 10 in contact with the inner surface. More specifically, the lower body portion 325 includes a lower outer case 326, a lower intermediate mold 327, and a lower inner mold 328.
하부 외부케이스(326)는 열전도성이 작은 합성수지 및 고무와 같은 재질을 이용하여 형성하여, 하부 외부케이스(326)의 내부에 배치되어 열을 발생하는 발열체(360)의 열이 외부로 전도되지 않도록 하여 빠르고 경제적으로 용기(10)를 가온 및 항온으로 유지할 수 있다. The lower outer case 326 is formed using a material such as synthetic resin and rubber having low thermal conductivity, so that the heat of the heating element 360 that is disposed inside the lower outer case 326 to generate heat does not conduct to the outside. It is possible to quickly and economically keep the container 10 at warm and constant temperature.
하부 중간금형(327)은 하부 외부케이스(326)의 내부에 삽입되도록 형성되며, 발열체(360)가 배치되는 공간이 마련된다. The lower intermediate mold 327 is formed to be inserted into the lower outer case 326 and has a space in which the heating element 360 is disposed.
하부 내부금형(328)은 하부 중간금형(327)에 마련된 배치 공간에 삽입되며, 각각의 용기(10)의 상부에서 그 내면이 접하는 하부 수용홈(332)들이 형성되어 있다. 참고로, 하부 내부금형(328)은 다양한 형태로 제공될 수 있는 용기(10)의 외형에 맞는 하부 수용홈(332)이 형성되어 있는 것으로 교체하여 사용할 수 있다. The lower inner mold 328 is inserted into an arrangement space provided in the lower intermediate mold 327, and lower receiving grooves 332 are formed at the upper portion of each container 10 to contact the inner surface thereof. For reference, the lower inner mold 328 may be used to replace the lower receiving groove 332 is formed to match the outer shape of the container 10 that can be provided in various forms.
하부 중간금형(327) 및 하부 내부금형(328)은 열전도성이 뛰어난 금속 금형으로 형성되어 있으며, 발열체(360)로부터 발생된 열이 쉽게 전도되고, 이에 하부 수용홈(332)에 삽입되는 용기(10)에 열을 골고루 전도할 수 있다. The lower intermediate mold 327 and the lower inner mold 328 are formed of a metal mold having excellent thermal conductivity, and the heat generated from the heating element 360 is easily conducted, and the container is inserted into the lower receiving groove 332. 10) can conduct heat evenly.
상부 몸체부(321)를 하부 몸체부(325)의 전면을 개방하도록 연 상태에서 하부 몸체부(325)에 형성된 하부 수용홈(332)에 용기(30)를 배치하고, 상부 몸체부(321)로 하부 몸체부(325)의 전면을 가리도록 닫아, 용기 수용부(330)에 용기(10)를 배치할 수 있다. The container 30 is disposed in the lower accommodating groove 332 formed in the lower body 325 while the upper body 321 is opened to open the front surface of the lower body 325, and the upper body 321 is disposed. The container 10 may be disposed in the container accommodating part 330 by closing the front surface of the lower body part 325.
이때, 상부 몸체부(321)의 내부에 배치된 복수개의 접촉 밀착편(323)들은 하부 수용홈(332)에서 수용하지 못한 용기(10)의 나머지 부분에 의해서 눌려 그 형태가 변하게 된다.At this time, the plurality of contact contact pieces 323 disposed inside the upper body portion 321 are pressed by the remaining portion of the container 10 that is not accommodated in the lower receiving groove 332 is changed in shape.
따라서, 상부 몸체부(321)와 하부 몸체부(325)가 닫히면, 상부 몸체부(321)가 용기(30)에 의해서 눌린 부분과 하부 몸체부(325)에 각각 형성된 하부 수용홈(325)이 하나의 용기 수용부(330)를 형성한다. Therefore, when the upper body portion 321 and the lower body portion 325 is closed, the upper body portion 321 is pressed by the container 30 and the lower receiving groove 325 formed in the lower body portion 325, respectively One container receiving portion 330 is formed.
이에, 상부 몸체부(321)를 하부 몸체부(325)의 전면을 개방하도록 연 상태에서 하부 몸체부(325)에 형성된 하부 수용홈(332)에 용기(10)를 배치하고, 상부 몸체부(321)로 하부 몸체부(325)의 전면을 가리도록 닫아, 용기 수용부(330)에 용기(10)를 배치할 수 있다. Thus, the container 10 is disposed in the lower receiving groove 332 formed in the lower body portion 325 in a state in which the upper body portion 321 is opened to open the front surface of the lower body portion 325, and the upper body portion ( The container 10 may be disposed in the container accommodating part 330 by closing the front surface of the lower body part 325 with 321.
그 후에, 진탕 운동부(150)는 베이스(110)에 대해서 용기수용 몸체부(320)를 진탕 운동 시킬 수 있으며, 진탕 운동은 상하좌우 운동이나 진동운동을 모두 포함할 수 있다. 따라서, 진탕 운동시에 상부 몸체부(321)와 하부 몸체부(325)가 상호 이격되는 것을 방지하는 클립 등으로 제공되는 고정부재(340)를 사용하여 결합할 수 있다. Thereafter, the shaking motion part 150 may shake the container housing body part 320 with respect to the base 110, and the shaking motion may include both up, down, left, and right motions or vibration motions. Therefore, the upper body portion 321 and the lower body portion 325 during the shaking motion can be combined using a fixing member 340 provided as a clip to prevent the mutual separation.
한편, 용기(10) 내부에 담긴 대상물질과 반응물질간에 혼합이 잘 이루어 지도록 진탕 운동을 수행하면서 용기(10)를 적정한 온도 본 실시예에서는 수용물질이 목표 효소반응 최적온도인 37℃까지 온도를 상승시켜, 일정 시간 동안 그 온도를 유지할 수 있다. On the other hand, the appropriate temperature of the container 10 while performing a shaking motion so that the mixing between the target material and the reactant contained in the container 10 is well made in this embodiment the temperature of the water to the optimum temperature of the target enzyme reaction 37 ℃ It can be raised to maintain its temperature for a period of time.
용기(10)를 가열하기 위하여 용기수용 몸체부(320)에는 발열체(360)가 배치될 수 있으며, 본 실시예에서는 설치의 편의상 하부 몸체부(325)의 내부에 배치하는 것이 바람직할 것이다. The heating element 360 may be disposed in the container housing body 320 to heat the container 10, and in this embodiment, it may be preferable to arrange the inside of the lower body part 325 for convenience of installation.
참고로, 본 실시예에 따른 용기수용 몸체부(320)는 제1 실시예에서 설명한 진탕 운동부(150)를 이용하여 용기(20)를 교반하는 것이 가능하며, 경우에 따라서는 별도의 진탕기를 사용하여 용기 내에 수용물질을 교반시킬 수도 있을 것이다.For reference, the container housing body 320 according to the present embodiment may agitate the container 20 by using the shaking motion unit 150 described in the first embodiment, and in some cases, a separate shaker is used. The agitation material may be stirred in the container.
상술한 바와 같이, 본 발명의 바람직한 실시예를 참조하여 설명하였지만 해당 기술분야의 숙련된 당업자라면 하기의 청구범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경시킬 수 있음을 이해할 수 있을 것이다.As described above, although described with reference to the preferred embodiment of the present invention, those skilled in the art various modifications and variations of the present invention without departing from the spirit and scope of the invention described in the claims below I can understand that you can.
본 발명에 따른 항온 교반장치 및 항온 교반장치를 이용한 교반방법은 용기에 수용된 수용물질을 효율적으로 교반할 수 있다. The stirring method using the constant temperature stirrer and the constant temperature stirrer according to the present invention can efficiently stir the receiving material contained in the container.
Claims (18)
- 용기에 수용된 수용물질을 교반하기 위한 항온 교반장치에 있어서,In the constant temperature stirrer for stirring the receiving material contained in the container,베이스;Base;상기 베이스 상부에 위치하며, 용기의 상하부를 덮어 수용하는 상부 몸체부 및 하부 몸체부를 포함하고, 상기 용기와 접하는 상기 상부 몸체부 및 상기 하부 몸체부의 내면은 상기 용기의 외면에 대응하는 용기 수용부를 형성하는 용기수용 몸체부; 및Located in the upper portion of the base, and includes an upper body portion and a lower body portion for covering the upper and lower parts of the container, the inner surface of the upper body portion and the lower body portion in contact with the container forms a container receiving portion corresponding to the outer surface of the container Container for receiving body portion; And상기 베이스에 대해서 상기 용기수용 몸체부를 진탕(shaking) 운동 시키는 진탕 운동부;를 구비하며,A shaking motion part configured to shake the container receiving body part with respect to the base;상기 용기와 접하는 용기 수용부의 내면을 통해서 상기 용기로 열을 전달하는 것을 특징으로 하는 항온 교반장치.Constant temperature agitation device, characterized in that for transferring heat to the vessel through the inner surface of the container receiving portion in contact with the vessel.
- 제1항에 있어서,The method of claim 1,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기와 접하는 고체 금형을 포함하며, 상기 고체 금형은 상기 용기의 외면에 밀착할 수 있도록 대응하여 형성된 수용홈을 포함하고, 상기 수용홈이 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 항온 교반장치. And a solid mold in contact with the container, wherein the solid mold includes a receiving groove correspondingly formed to be in close contact with the outer surface of the container, wherein the receiving groove forms a part of the container accommodation portion. Device.
- 제1항에 있어서,The method of claim 1,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기과 접하는 유동 팩을 포함하며, 상기 유동 팩의 내부에는 열을 저장할 수 있는 유체가 충진되며, 상기 유동 팩의 외면은 상기 용기의 외면에 밀착할 수 있도록 변형되어 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 항온 교반장치. And a flow pack in contact with the container, wherein the fluid pack is filled with a fluid capable of storing heat, and an outer surface of the flow pack is deformed to be in close contact with the outer surface of the container to form a portion of the container accommodation portion. Constant temperature stirrer, characterized in that.
- 제3항에 있어서,The method of claim 3,상기 유동 팩은 탄성 변형이 가능한 재질로 형성되는 것을 특징으로 하는 항온 교반장치. The flow pack is a constant temperature stirring device, characterized in that formed of a material capable of elastic deformation.
- 제1항에 있어서,The method of claim 1,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기과 접하는 복수개의 접촉 밀착편을 포함하며, 상기 접촉 밀착편은 상기 상부 몸체부 또는 상기 하부 몸체부에 대해 변위 가능하게 지지되고, 상기 접촉 밀착편은 상기 용기의 외면에 밀착할 수 있도록 변위되면서 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 항온 교반장치. It includes a plurality of contact contact pieces in contact with the container, the contact contact piece is supported so as to be displaceable relative to the upper body portion or the lower body portion, the contact contact piece is displaced to be in close contact with the outer surface of the container Constant temperature stirring device, characterized in that to form a portion of the container receiving portion.
- 제1항에 있어서,The method of claim 1,상기 상부 몸체부와 상기 하부 몸체부는 분리되어 제공되거나, The upper body portion and the lower body portion is provided separately,상기 상부 몸체부는 상기 하부 몸체부의 상면을 노출할 수 있도록 상기 하부 몸체부와 상대 이동 가능하게 결합되는 것을 특징으로 하는 항온 교반장치. The upper body portion is a constant temperature stirring device, characterized in that coupled to the lower body portion and relatively movable so as to expose the upper surface of the lower body portion.
- 제1 항에 있어서,According to claim 1,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는 발열체를 포함하며, 상기 발열체는 상기 용기 수용부에 인접하여 배치되는 것을 특징으로 하는 항온 교반장치. At least one of the upper body portion and the lower body portion includes a heating element, the heating element is characterized in that the heating element is disposed adjacent to the container receiving portion.
- 제1항에 있어서,The method of claim 1,상기 용기수용 몸체부는 상기 진탕 운동부로부터 탈착가능한 것을 특징으로 하는 교반장치. The container holding body portion is agitated, characterized in that detachable from the shaking motion portion.
- 진탕 운동이 가능한 진탕운동 베이스 상에 결합되어 용기에 수용된 수용물질을 교반하기 위한 교반장치용 용기수용 몸체부에 있어서,In the container receiving body portion for the stirring device for stirring the receiving material contained in the container coupled to the shaking motion base capable of shaking motion,용기의 상하부를 덮어 수용하는 상부 몸체부 및 하부 몸체부를 포함하고,It includes an upper body portion and a lower body portion for covering the upper and lower parts of the container,상기 용기와 접하는 상기 상부 몸체부 및 상기 하부 몸체부의 내면은 상기 용기의 외면에 대응하는 용기 수용부를 형성하고,The inner surface of the upper body portion and the lower body portion in contact with the container forms a container receiving portion corresponding to the outer surface of the container,상기 용기와 접하는 용기 수용부의 내면을 통해서 상기 용기로 열을 전달하는 것을 특징으로 하는 교반장치용 용기수용 몸체부.The container housing body portion for stirring device, characterized in that for transferring heat to the vessel through the inner surface of the container receiving portion in contact with the vessel.
- 제9항에 있어서,The method of claim 9,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기과 접하는 고체 금형을 포함하며, 상기 고체 금형은 상기 용기의 외면에 밀착할 수 있도록 대응하여 형성된 수용홈을 포함하고, 상기 수용홈이 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 항온 교반장치용 용기수용 몸체부.And a solid mold contacting the container, wherein the solid mold includes a receiving groove correspondingly formed to be in close contact with the outer surface of the container, wherein the receiving groove forms a portion of the container accommodation portion. Body part for container.
- 제9항에 있어서,The method of claim 9,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기과 접하는 유동 팩을 포함하며, 상기 유동 팩의 내부에는 열을 저장할 수 있는 유체가 충진되며, 상기 유동 팩의 외면은 상기 용기의 외면에 밀착할 수 있도록 변형되어 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 항온 교반장치용 용기수용 몸체부.And a flow pack in contact with the container, wherein the fluid pack is filled with a fluid capable of storing heat, and an outer surface of the flow pack is deformed to be in close contact with the outer surface of the container to form a portion of the container accommodation portion. A container housing body portion for a constant temperature stirring device, characterized in that.
- 제11항에 있어서,The method of claim 11,상기 유동 팩은 탄성 변형이 가능한 재질로 형성되는 것을 특징으로 하는 항온 교반장치용 용기수용 몸체부. The flow pack is a container housing body portion for a constant temperature stirring device, characterized in that formed of a material capable of elastic deformation.
- 제9항에 있어서,The method of claim 9,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기과 접하는 복수개의 접촉 밀착편을 포함하며, 상기 접촉 밀착편은 상기 상부 몸체부 또는 상기 하부 몸체부에 대해 변위 가능하게 지지되고, 상기 접촉 밀착편은 상기 용기의 외면에 밀착할 수 있도록 변위되면서 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 항온 교반장치용 용기수용 몸체부.It includes a plurality of contact contact pieces in contact with the container, the contact contact piece is supported so as to be displaceable relative to the upper body portion or the lower body portion, the contact contact piece is displaced to be in close contact with the outer surface of the container A container housing body portion for a constant temperature stirring device, characterized in that to form a portion of the container receiving portion.
- 제9에 있어서,The method according to claim 9,상기 하부 몸체부 및 상기 상부 몸체부 중 적어도 하나는 발열체를 포함하며, 상기 발열체는 상기 용기 수용부에 인접하여 배치되는 것을 특징으로 하는 항온 교반장치용 용기수용 몸체부.At least one of the lower body portion and the upper body portion includes a heating element, the heating element is a container housing body portion for a constant temperature stirring device, characterized in that disposed adjacent to the container receiving portion.
- 교반장치를 이용하여 용기에 수용된 수용물질을 교반하기 위한 교반방법에 있어서,In the stirring method for stirring the water contained in the container using a stirring device,용기의 상하부를 덮어 수용하는 상부 몸체부 및 하부 몸체부를 포함하고, 상기 용기와 접하는 상기 상부 몸체부 및 상기 하부 몸체부의 내면은 상기 용기의 외면에 대응하는 용기 수용부를 형성하는 용기수용 몸체부를 구비하는 교반장치를 제공하는 단계;An upper body portion and a lower body portion covering and holding the upper and lower portions of the container, and inner surfaces of the upper body portion and the lower body portion contacting the container include a container accommodating body portion forming a container accommodating portion corresponding to an outer surface of the container. Providing a stirring device;상기 상부 몸체부 및 상기 하부 몸체부 사이에 용기를 위치시키는 단계;Positioning a container between the upper body portion and the lower body portion;상기 상부 몸체부 및 상기 하부 몸체부의 대향하는 내면을 상기 용기의 외면에 밀착시키는 단계;Bringing the inner surface of the container into close contact with the outer surface of the upper body portion and the lower body portion;상기 용기수용 몸체부를 진탕(shaking) 운동 시키는 단계; 및Shaking the container housing body; And상기 용기와 접하는 상기 용기 수용부의 내면을 통해서 상기 용기를 특정 온도도 가온하는 단계;Heating the vessel to a specific temperature through an inner surface of the vessel receiving portion in contact with the vessel;를 구비하는 교반방법.Stirring method provided with.
- 제15항에 있어서,The method of claim 15,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기와 접하는 고체 금형을 포함하며, 상기 고체 금형은 상기 용기의 외면에 밀착할 수 있도록 대응하여 형성된 수용홈을 제공하는 것을 특징으로 하는 교반방법.And a solid mold in contact with the container, wherein the solid mold provides a receiving groove correspondingly formed to be in close contact with the outer surface of the container.
- 제15항에 있어서,The method of claim 15,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기와 접하는 유동 팩을 포함하며, 상기 유동 팩의 내부에는 열을 저장할 수 있는 유체를 충진하고, 상기 유동 팩의 외면은 상기 용기의 외면에 밀착할 수 있도록 변형 가능한 것을 특징으로 하는 교반방법.And a flow pack in contact with the container, wherein the inside of the flow pack is filled with a fluid capable of storing heat, and an outer surface of the flow pack is deformable to be in close contact with the outer surface of the container.
- 제15항에 있어서,The method of claim 15,상기 상부 몸체부 및 상기 하부 몸체부 중 적어도 하나는,At least one of the upper body portion and the lower body portion,상기 용기과 접하는 복수개의 접촉 밀착편을 포함하며, 상기 접촉 밀착편은 상기 상부 몸체부 또는 상기 하부 몸체부에 대해 변위 가능하게 지지되고, 상기 접촉 밀착편은 상기 용기의 외면에 밀착할 수 있도록 변위되면서 상기 용기 수용부의 일부를 형성하는 것을 특징으로 하는 교반방법.It includes a plurality of contact contact pieces in contact with the container, the contact contact piece is supported so as to be displaceable relative to the upper body portion or the lower body portion, the contact contact piece is displaced to be in close contact with the outer surface of the container Forming a portion of the container receiving portion, characterized in that the stirring method.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090035004A KR101076699B1 (en) | 2009-04-22 | 2009-04-22 | Device for incubating shaker and shaking method using the device |
KR10-2009-0035004 | 2009-04-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010123205A2 true WO2010123205A2 (en) | 2010-10-28 |
WO2010123205A3 WO2010123205A3 (en) | 2011-01-06 |
Family
ID=43011563
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2010/001792 WO2010123205A2 (en) | 2009-04-22 | 2010-03-24 | Constant temperature agitating device and agitating method using the same |
Country Status (2)
Country | Link |
---|---|
KR (1) | KR101076699B1 (en) |
WO (1) | WO2010123205A2 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8550696B2 (en) * | 2006-03-09 | 2013-10-08 | Eppendorf Ag | Laboratory mixer and vortexer |
US20130265845A1 (en) * | 2010-05-03 | 2013-10-10 | Eppendorf Ag | Connection for a Temperature-Controllable Exchangeable Block |
WO2015158852A1 (en) * | 2014-04-16 | 2015-10-22 | Sartorius Stedim Biotech Gmbh | Mixing and thawing device |
EP2817574A4 (en) * | 2012-02-21 | 2016-01-27 | Anthrogenesis Corp | Devices and methods for thawing biological material |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002361061A (en) * | 2001-06-12 | 2002-12-17 | Japan Tobacco Inc | Shaking apparatus |
KR200382867Y1 (en) * | 2005-02-16 | 2005-04-25 | 오병호 | The shaker of chemical elements in the laboratory |
KR200388445Y1 (en) * | 2005-04-18 | 2005-06-29 | 한덕희 | Shaker |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3580864B2 (en) * | 1994-08-11 | 2004-10-27 | 倉敷紡績株式会社 | Stirrer |
-
2009
- 2009-04-22 KR KR1020090035004A patent/KR101076699B1/en active IP Right Grant
-
2010
- 2010-03-24 WO PCT/KR2010/001792 patent/WO2010123205A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002361061A (en) * | 2001-06-12 | 2002-12-17 | Japan Tobacco Inc | Shaking apparatus |
KR200382867Y1 (en) * | 2005-02-16 | 2005-04-25 | 오병호 | The shaker of chemical elements in the laboratory |
KR200388445Y1 (en) * | 2005-04-18 | 2005-06-29 | 한덕희 | Shaker |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8550696B2 (en) * | 2006-03-09 | 2013-10-08 | Eppendorf Ag | Laboratory mixer and vortexer |
US20130265845A1 (en) * | 2010-05-03 | 2013-10-10 | Eppendorf Ag | Connection for a Temperature-Controllable Exchangeable Block |
US9016929B2 (en) * | 2010-05-03 | 2015-04-28 | Eppendorf Ag | Apparatus for mixing and controlling the temperature of laboratory vessel contents |
EP2817574A4 (en) * | 2012-02-21 | 2016-01-27 | Anthrogenesis Corp | Devices and methods for thawing biological material |
WO2015158852A1 (en) * | 2014-04-16 | 2015-10-22 | Sartorius Stedim Biotech Gmbh | Mixing and thawing device |
US10363528B2 (en) | 2014-04-16 | 2019-07-30 | Sartorius Stedim Biotech Gmbh | Mixing and thawing device |
Also Published As
Publication number | Publication date |
---|---|
WO2010123205A3 (en) | 2011-01-06 |
KR101076699B1 (en) | 2011-10-26 |
KR20100116347A (en) | 2010-11-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2010123205A2 (en) | Constant temperature agitating device and agitating method using the same | |
WO2013018968A1 (en) | Facial cleansing apparatus | |
EP3285574B1 (en) | Medical sample transportation container | |
WO2020060056A1 (en) | Automatic sterile stool collector | |
JP4953769B2 (en) | Cell culture device and cell culture cassette | |
US7442538B2 (en) | Modular system comprising multiple automated mini-bioreactors for high-throughput screening (HTS) in biotechnology | |
WO2012066500A1 (en) | Flow-through apparatus | |
WO2017141394A1 (en) | Reaction device capable of performing centrifugation | |
WO2017047986A1 (en) | Cell spheroid cultivator, and cell spheroid culture system containing same | |
CN117385000A (en) | Multitasking parallel nucleic acid extraction and transformation method and device thereof | |
WO2014183251A1 (en) | Stirrer | |
US20200191813A1 (en) | Laboratory instrument and insertable network instrument | |
JP4817362B2 (en) | Apparatus and method for uniform cell seeding on a substrate having a three-dimensional surface | |
WO2017007278A1 (en) | Automated cell cultivation device, and method for operating cultivation device | |
KR102318710B1 (en) | Cell culture apparatus and cell culture method | |
WO2010101358A2 (en) | Instrument for culturing cells or tissue | |
CN216704182U (en) | Digital display heating magnetic stirrer for stirring skin care product | |
WO2021112546A1 (en) | Bio-reaction stirrer using magnetic force | |
CN208115736U (en) | A kind of chemical experiment reactor tank convenient for observation | |
WO2018097510A1 (en) | Bioreaction container | |
WO2012074206A2 (en) | Disposable container for the culture of microorganisms using a magnet and a culture apparatus | |
CN105176790B (en) | Microarray hybridization elution instrument | |
WO2022146028A1 (en) | Air bubble removal device for removing air bubbles from medium and medium circulating cell cultivation system comprising same | |
WO2024049165A1 (en) | Sample pretreatment device | |
WO2020209417A1 (en) | Rotary gel nail container storage case |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 10767220 Country of ref document: EP Kind code of ref document: A2 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 10767220 Country of ref document: EP Kind code of ref document: A2 |