WO2010084190A1 - Soupape fluidique à usage unique et systèmes microfluidiques intégrant ladite soupape - Google Patents

Soupape fluidique à usage unique et systèmes microfluidiques intégrant ladite soupape Download PDF

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Publication number
WO2010084190A1
WO2010084190A1 PCT/EP2010/050801 EP2010050801W WO2010084190A1 WO 2010084190 A1 WO2010084190 A1 WO 2010084190A1 EP 2010050801 W EP2010050801 W EP 2010050801W WO 2010084190 A1 WO2010084190 A1 WO 2010084190A1
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WO
WIPO (PCT)
Prior art keywords
film
fluid
formation
actuators
high absorption
Prior art date
Application number
PCT/EP2010/050801
Other languages
English (en)
Inventor
Antonio Ricco
Jose L Garcia Cordero
Jens Ducree
Luke Lee
Fernando Benito Lopez
Original Assignee
Dublin City University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0901115A external-priority patent/GB2467298A/en
Priority claimed from GB0902980A external-priority patent/GB2468111A/en
Application filed by Dublin City University filed Critical Dublin City University
Publication of WO2010084190A1 publication Critical patent/WO2010084190A1/fr

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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
    • F16K99/0015—Diaphragm or membrane valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/008—Multi-layer fabrications

Definitions

  • the present invention relates to valves for controlling the flow of a fluid through a flow path within a microfluidic device.
  • the flow path is from one formation to another formation.
  • the formations may be channels or reservoirs or other defined volumes within the microfluidic device for operably accommodating a fluid.
  • the invention more particularly relates to a single use valve for use in microfluidic systems, actuation of the valve enabling fluid flow from one formation of the microfluidic system to another formation of the system.
  • the invention further relates to a microfluidic reagent storage device comprising at least one single use valve.
  • the invention further relates to point of care (POC) diagnostic devices comprising two or more microfluidic formations separated from one another by single use valves.
  • POC point of care
  • Point-of-care (POC) diagnostic devices are known. Such devices are intended for use at or near the patient being tested. It is envisaged that such devices will revolutionize and improve global public health by diagnosing diseases in a timely manner, preventing epidemics, controlling chronic health conditions, tailoring treatments, and decreasing national health system costs. As it is intended that such POC devices will be used outside controlled laboratory conditions and may be used in deprived- resource settings it is important that the devices have the capacity to be portable, possible disposable, are available at low cost, can be used simply and are sufficiently rugged for their intended environment of use. They also need to deliver assay results with similar sensitivity, reproducibility, and selectivity to centralized laboratory tests. Finally, it is desirable that POC devices should operate with minimal, non-expert operator attention.
  • microfluidic and lab-on-a-chip technologies are being used to provide such POC diagnostic systems.
  • the lab-on-a-chip vision is to miniaturize clinical laboratory processes, integrating them onto disposable units the size of a credit card using minute amounts of complex samples and precious reagents.
  • These autonomous and integrated chips would consist of different modules or components that would handle a complex sample such as blood, preparing it and mixing it with the necessary reagents to produce a signal that can be read by a miniaturized, even an on-chip, detection system.
  • Microfluidic valves and pumps are ubiquitous in integrated microfluidic systems, but fluid actuation and control can greatly add to the fabrication costs of integrated microsystems: external actuators may be needed to drive them or their implementation into manufacturing processes may be a costly engineering challenge. There is therefore a number of problems with the practical use of microfluidic systems within POC devices.
  • a microfluidic device comprising a first and second formations separated from one another by a single use valve.
  • the microfluidic device is a multilayer system with the first and second formations are desirably provided in first and second layers of the fluidic device and a valve is provided to control fluid passage therebetween.
  • the valve provides a separating membrane between the two formations and on photo-actuation is configured to rupture to provide a fluid path between the two formations.
  • the valve is provided in a third layer separating the first and second layers.
  • the third layer may optimally be a film such as a plastic film having a structural integrity defining a continuous sheet.
  • the sheet may be provided as a discrete leaf which may be stored in a stack or roll prior to use.
  • the third layer is desirably laid down onto the first or second layers as a single solid element for example by unrolling it from its stored configuration.
  • the valve is desirably formed by providing at a predefined location within or on the third layer an absorber which on exposure to a provided electromagnetic radiation signal will heat and effect a localised melting of the third layer to provide fluid communication between the first and second formations.
  • a plurality of valves are provided by patterning the third layer at predefined locations with an absorbing material.
  • the one or more valves are desirably provided on the third layer.
  • the valves are provided by printing the absorber material onto the third layer.
  • laser printing is a particularly effective means of depositing an absorber material at predefined locations on the third layer, and can be used for depositing one or more dots of absorber material at predefined locations on the third layer.
  • the absorber may be provided on an upper or lower surface of the third layer.
  • the absorber is provided on a lower surface such that any effect of the material properties of the absorber material on the reagents is minimised. It will however be appreciated that it is not intended to limit the present teaching to the exact placement of the absorbing materials relative to the stored fluid within the device.
  • the dot can either be proximal or distal relative to the stored fluid. In the event that there were reasons for concern about interaction between the fluid or components of the fluid and the material comprising the dot, then it would be advantageous to have the dot on the distal side of the third layer relative to the stored fluid. Whether this is necessary depends on the details of the fluid's components, and the particular material chosen for the dot as well.
  • the absorbing material could be encapsulated or sandwiched between first and second films.
  • first and second films For example, if the dots were printed or otherwise deposited on a first plastic film, a second plastic film could be laminated onto the first film (using for example thermal means), thereby sandwiching and effectively encapsulating the dots.
  • the absorber is desirably formed from a material having a greater or higher absorption characteristic to the material forming the third layer or in other arrangements to the surrounding areas of the device.
  • the absorber will preferentially heat relative to its environment. In this way selective and controlled melting at predefined locations may be effected. This localised preferential heating provides for a highly controlled generation of a fluid path between first and second formations.
  • the first formation may be segmented having first and second individual segments separated from one another by the second formation.
  • first and second valves are provided between each of the first segment and the second formation, and the second formation and the second segment respectively.
  • the first formation may define a reservoir.
  • the first formation is fabricated to have a volume sufficient to contain a predefined volume of a fluid.
  • the first formation comprises an entry port and an exit port, control of fluid egress from the exit port being effected by a valve coincident with the exit port, and wherein on introduction of a fluid into the reservoir the entry port is sealed to prevent evaporation of the fluid from the reservoir.
  • a point of care device comprising: a multilayer microfluidic device comprising first and second formations provided in first and second layers of the microfluidic device and separated from one another by a single use valve provided in a third layer separating the first and second layers, the valve comprising an absorber material provided on a localised region of the third layer; a source of electromagnetic radiation for directing an electromagnetic signal onto the absorber and wherein on exposure of the absorber to a provided electromagnetic signal, the absorber material heats and effects a localised melting of the third layer to provide fluid communication between each of the first and second formations.
  • Figure 1A is a schematic representation of single use valves provided in accordance with the present teaching showing how segments of a formation provided in a first layer may be connected with one another by formations provided in a second layer.
  • Figure 1 B is a sectional view showing the separation of the first and second segments of Figure 1A.
  • Figure 1 C is a side cross sectional view of details of Figures 1 A in use.
  • Figure 2 is a schematic showing operation of the device of Figure 1 with Figure 2A showing the loading of liquid into a first segment, Figure 2B showing the opening of a first valve and the flow of the liquid into the second formation, Figure 2C showing the opening of the second valve to allow for fluid communication between the second formation and the second segment.
  • Figure 3 shows in schematic form the fabrication or formation of a microfluidic reservoir in accordance with the present teaching.
  • Figure 4 shows in schematic form a centrifugal microfluidic system comprising first and second formations separated by a valve
  • FIG. 5 shows in schematic form the use of a valve in accordance with the present teaching to enable the on-board storage of two solutions.
  • Figure 6 shows various arrangements for provision of outlets from a reservoir or formation in accordance with the present teaching.
  • valve arrangement for use in microfluidic systems will now be described to assist in an understanding of the present teaching.
  • fabrication of a single-use valve based on laser printing technology will be described.
  • Such valves are provided in a normally closed state and may be opened with a single laser shot or pulse, to allow for the flow of a fluid within the microfluidic system.
  • a system for the storage of liquid reagents in sealed reservoirs for up to 30 days with no significant evaporation will also be described.
  • Figure 1 shows in schematic form an example of a multilayer microfluidic device 100 incorporating first 110A and second 110B microfluidic single- use valves.
  • First 120 and second 130 formations are provided in first 125 and second 135 layers of the device.
  • the first formation 120 is, in this arrangement, segmented into first 121 and second 122 segments.
  • the second formation 130 overlaps at least partially with each of the first and second segments and is separated from each of the first 121 and second segments by a third layer 140.
  • the third layer 140 is formed from a plastic polymer foil with laser-printed dots 141 and is sandwiched between each of the first 125 and second 135 layers.
  • the valves 110A, 110B are formed by the provision of the laser printed dots 141 on the third layer 140 and are defined at the respective overlap between each of the first segment and the second formation, and the second formation and the second segment. In this way, on opening the valves, the second formation provides a fluid interconnect between the first and second segments. While the dots 141 are described as being laser printed on the third layer 140, it should be appreciated that it is not intended to limit the teaching of the present application to laser printing. The applicant envisages that the dots 141 may be deposited using any suitable means. Furthermore it will be appreciated that dots are an exemplary arrangement of discrete high absorption actuators, and that the teaching is not to be considered as being limited to the geometry of a dot.
  • each laser-printed dot 141 is to absorb optical energy from an actuating light source such as a laser diode, rapidly heat and effect a perforation of the plastic foil 140 by melting it within a localised region coincident with the location of the dots 141 to form an outlet 112 between the two formations.
  • the laser printer dots 141 operate as actuators for opening the valves.
  • the absorption properties of the dots 141 are such that it will preferentially absorb the incident radiation relative to the surrounding areas of the plastic foil or film that forms the third layer 140, the integrity of the remaining areas of the third layer 140 are unaffected.
  • a fluid is provided into the first segment 121.
  • a source of electromagnetic radiation desirably a controlled pulse signal of predefined duration and intensity, is provided in this arrangement by a laser diode 250.
  • the laser diode 250 is positioned to point at the first valve 110A.
  • the absorption of a short pulse of light 255 by the valve 110A effects a localised heating of the third layer and melts the plastic polymer foil that forms that layer in that localised region to form an outlet 112. This outlet provides a fluid path between the first segment 121 and the second formation 130.
  • Step 210 the laser diode 250 is then moved to the second valve 110B and the operation repeated. Liquid then can be moved through the second formation 130 into the second segment 122. Once the fluid path between the first and second segments through the second formation is generated the laser diode may be turned off (Step 220)- this does not require the flow of the actual fluid, simply that a path is fabricated. It will be appreciated that as the strength of the applied signal needs only to be sufficient to effect a heating of the absorber material the laser can be left on while moving it from the first valve 110A to the second valve 110B without effecting any damage to the intervening structure- the strength of the laser diode being insufficient to effect a melting of the third layer in the absence of the absorber materials.
  • first and second valves are described.
  • the need for two valves may not be obvious: one of the two could be perforated before assembly, reducing the complexities of positioning and control of the laser, but adding an additional step to the fabrication process. Pre-perforation of one dot would also eliminate the redundancy against leakage or slow permeation of water vapour afforded by two dots/valve. It will however be appreciated that it is not intended to limit the application of the present teaching to an arrangement that requires sequential opening of first and second valves to enable fluid communication between first and second segments within the same layer.
  • the first formation may be dimensioned to define a reservoir 300.
  • the formation is fabricated to have a volume sufficient to contain a predefined volume of a fluid.
  • the formation comprises an entry port 310 and an exit port 320 with control of fluid egress from the exit port 320 being effected by a valve 330 coincident with the exit port.
  • the valve is provided- similarly to that described above, by a localised well-defined region of a high absorbing actuator (printed dot) which on exposure to incident EM radiation will heat and effect a localised melting of the third layer 340 on which it is disposed. Fluid communication between the reservoir 300 and a connecting formation 350 provided below the valve is thereby enabled and the fluid within the reservoir may flow out of the reservoir to other regions of the device.
  • a high absorbing actuator printed dot
  • the structure of the reservoir is first defined within the first layer.
  • This reservoir is defined in the upper layer and can be designed to hold any fluid volume.
  • the valve for this storage reservoir is laser-printed at its peripheral end and at the intersection with a microfluidic formation 350 that connects it to the rest of the microfluidic system.
  • the barrier properties of the foil to store liquid reagents as was described above are exploited.
  • reagents 360 are loaded into the reservoir and encapsulated using pressure-sensitive adhesive (PSA) film 370 or other suitable sealing means. This film seals tightly the storage reservoir and prevents evaporation. Liquid from the container 300 can be cleanly released into the formation by centrifugal or capillary actuation.
  • PSA pressure-sensitive adhesive
  • a centrifugal microfluidic "lab-on- a-disc" cartridge with two chambers 400, 410 connected by a microfluidic channel 420 such as that schematically shown in Figure 4 was fabricated. Flow through the channel was controlled by provision of a valve 430 between the first chamber 400 and the channel 420.
  • the first and second chambers were fabricated on a substrate 440 that was configured to be rotated on a spindle 441. In this exemplary arrangement the substrate 440 is a planar disc structure.
  • the first and second chambers were radially arranged relative to one another with the first chamber being provided proximal to the spindle and the second chamber 410 distally provided.
  • a fluid solution 450 is initially loaded into the first chamber- Figure 4A.
  • the substrate 440 was rotated at different speeds and no leakage was observed through the valve even while spinning at 5000 rpm.
  • the disc was then stopped and light from a laser diode was aimed at the laser- printed area- i.e. the valve 430, creating a communication port between the first chamber 400 and the channel 420 in less than 1 second.
  • the disc was spun again and the fluid solution was fully transferred to the second chamber 410 under the influence of the biasing centrifugal forces resultant from rotation of the substrate 440- Figure 4B.
  • the device of Figure 4 was fabricated using multi-layer lamination.
  • a CO2 laser Laser Micromachining LightDeck, Optec, Belgium
  • a thermal roller laminator Teitan-110, GBC Films, USA
  • a laser-printer (resolution:
  • Connecting channels were cut from an 80- ⁇ m thick layer of PSA (AR9808, Adhesives Research, Ireland) and laminated onto a 250- ⁇ m poly(methylmethacrylate), PMMA, support layer (GoodFellow, UK). The width of the connecting microfluidic channel was measured to be approximately 400 ⁇ m.
  • This assembly of channels constituted the connecting layer in both devices.
  • the upper chambers shown in Figure 4 were laser-cut from a 250- ⁇ m PMMA sheet. These layers were then laminated onto the connecting layer. Finally, a layer of PSA with laser-cut holes that function as vents was laminated on top of the chambers. It will be appreciate that the provision of vent holes is particularly useful in the filling of chambers or channels that were originally empty.
  • vent holes are not critical in that one can also construct such a pair of chambers with no holes in either of them. In such a scenario, the rotation velocity may need to be higher in order to generate enough pressure that the fluid flows into the empty chamber through the valve, and air bubbles backwards through the hole (just like pouring water from a bottle with a narrow mouth).
  • FIG. 5 illustrates the system design for the on-board reagent-storage device 500.
  • two reservoirs 505, 510 are defined on a rotatable substrate 515 and located near the centre of the substrate 515. It will be appreciated that as the radial motion of the substrate 515 induces movement from a chamber proximally located to the centre of rotation of the substrate towards a chamber distally located from that centre of rotation that it is important in such an arrangement that the reservoirs are arranged relative to one another such that on rotation of the substrate 515 that the fluid has an outward path of flow.
  • two solutions 506, 511 were loaded into the reservoirs 505, 511 respectively and sealed with PSA-coated film.
  • the first and second reservoirs were coupled to a mixing chamber 520 via first 507 and second 512 channels respectively. Flow of liquid from the chambers to the channels is controlled by first 508 and second 513 valves.
  • valves In operation, the fluid solutions were provided into each of the reservoirs.
  • the valves are then opened and the disc spun to displace the liquids into a mixing chamber, as shown in Figure 5B, to form a mixed volume 550. It was noted that when in the reservoirs that the stored solutions did not evaporate for a period of 30 days, and suitable polymers could extend this significantly.
  • the valves prevent fluid leakage at rotation rates of at least 5000 rpm (corresponding to 840xg).
  • microfluidic valves which allow controlling the photo-thermal activations of microfluidic valves selectively upon exposure to a specific wavelength of laser pulses.
  • the resonant frequency-based photo-thermal activation can deposit energy selectively and the thermal confinement particular to nanoplasmonic physics allows a minimization of the heat transfer (i.e. flow) from the plasmonic nanoparticles to the surrounding areas so as to provide for the localised perforation for generation of a fluid path.
  • such localized outlets 600 defined within the same reservoir 610 can be used for aliquoting or volume splitting of smaller volumes of a fluid from a larger volume, the routing of fluid from the same reservoir to different channels by addressing of spatially separated outlets or the sequential release of liquid from a reservoir by the sequential addressing of individual ones of the outlets.
  • Figure 6A shows an arrangement whereby a plurality of outlets 600 are provided on the same axis which is substantially perpendicular to the axis of the induced centrifugal force 605.
  • a plurality of outlets 600 are provided on the same axis which is substantially perpendicular to the axis of the induced centrifugal force 605.
  • the liquid within that reservoir and along that axis of the outlets 605 will be experiencing the same force.
  • one of a plurality of available channels 615 can be opened, and a portion of the volume of liquid within the reservoir 610 will exit the reservoir and follow that path.
  • Each of the channels 615 can be directed to the same or different destination such that routing of fluid within the microti iridic device can be achieved.
  • the liquid that is in a lower region 620 of the reservoir is biased there by the applied centrifugal force and will therefore not escape from an opened outlet.
  • the level of the outlets relative to the length of the reservoir can therefore be used to redirect specific portions of the fluid.
  • FIG. 6B A further example of this is shown in Figure 6B where a number of outlets are provided along the longitudinal axis of the reservoir.
  • selective opening of individual ones of these outlets will release specific volumes from the reservoir.
  • outlet 600A is opened only fluid above that outlet will escape.
  • the controlled opening of a plurality of provided outlets can therefore provide for a controlled release of a specific portion of the fluid from the reservoir.
  • This is particularly useful in the context of a feedback system where an initial separation of the fluid is effected through centrifugal rotation and based on feedback optical analysis to determine the transition points within the reservoir, a decision can be made as to which outlet should be opened to separate the constituents.
  • the ability to spatially distinguish between highly localised outlets and to selectively actuate these allows for such separation.
  • This readout of the phase interface can be done dynamically during the rotation of the device and the same or different optical device that is used for the ultimate laser ablation step to generate the outlet can be used for the optical analysis.
  • Such dynamic control of the fluid paths within a microfluidic system is a particularly advantageous aspect of a device provided in accordance with the present teaching. It will be appreciated that a plurality of individual target outlets can be provided radially along the same axis, such as shown in Figure 6C. In this arrangement selective movement of the device used for the laser ablation can be used to controllably define which of the plurality of outlets could be opened.
  • a controller in conjunction with the laser diode (or other source of the necessary radiation to provide the photothermal actuation of the device) can be used to selectively redirect specific volumes from specific locations within the reservoir out of the reservoir to predefined destinations.
  • the location of these outlets can be precisely defined and as such the ultimate volume that is redirected can be also known to a high degree of precision.
  • Figure 6C has particular application where the reservoir 610 provides a buffer solution that is needed in sequential steps of a test.
  • the buffer solution can be released sequentially to a separate chamber- not shown- where it can be mixed with or otherwise used with different reagents in strictly controlled volumes.
  • the release of the fluid is defined in time (i.e. the ultimate destination is the same), whereas the arrangement of Figures 6A and 6B allow for the routing to be defined in both a time sequence and/or a destination address.
  • FIG. 6D shows a further arrangement where the outlet is provided having an extended dimension to the highly localised pattern that has heretofore been described.
  • a stripe 650 of material defines the outlet.
  • This stripe of material is defined in this arrangement along a radial path within the reservoir 610 that is parallel with the axis of the induced centrifugal force 605.
  • this stripe pattern is just a further example of the type of geometry that can be achieved by the highly localised positioning of regions of higher absorption within the context of the present teaching.
  • a fluidic device comprising first and second formations separated from one another by one or more localised photoactuable separators.
  • These separators are distinct elements that define a region of high absorption within the device which on exposure to radiation are responsive to the photoactuation to transform from a first closed state to a second open state, adoption of the open state defining a fluid path between each of the first and second formations.
  • the highly defined geometry of such separators allows for highly controlled generation of fluid passages within the device which can be used to generate one or more independently controllable fluid paths within the fluidic devices.
  • separators which may be individually addressed and ablated, it is possible to provide for a controlled linking between individual fluid paths within the microfluidic device it is possible to provide a highly controlled movement of fluid within the device. This can be used to provide for the storage and or mixing of fluids. It can also be used to effect movement of fluids in both vertical and horizontal directions through the device.
  • the separators have been described with reference to a single use valve or a dot of an absorber material that is provided onto a substrate. It will be appreciated that such separators provide a rupturable membrane between each of the two channels that are initially separated and are exemplary of a surface having a discrete high absorption actuator provided thereon.
  • the separator By rupturing the membrane, achieved desirably through a preferential heating of the membrane relative to its immediate surroundings and a subsequent perforation of the membrane through that heating, it is possible to generate an aperture or fluid communication path between each of the first and second channels (formations). Such perforation results in a destruction of the integrity of the membrane at that location and in this way the separator can be considered a single use valve.
  • the fluidic device is desirably provided on a rotatable substrate, rotation of which generates a centrifugal force which biases the flow of a fluid from one portion of the device to another portion.
  • the rupturing of the membrane may be achieved concurrently with the rotation of the device or could be effected while the substrate is stationary.
  • first and second formations arranged one above the other, by suitably patterning a lower surface of the first formation or an upper surface of the second formation with one or more regions of high absorption it is possible to effect a preferential rupturing of that portion of the surface to provide a fluid path.
  • first and second formations are co- planar it is possible to simply separate them within the same plane by a region of high absorption which on photoactuation will ablate and allow fluid from a first formation to pass into a second formation. This ablation may result in a melting or evaporation of the region separating the first and second formations.
  • the use of the exemplary described printed valve technology facilitates the design and fabrication of fully integrated and automated lab-on-a-chip cartridges that require pressure-resistant valves or long-term reagent storage.
  • One key advantage is the absence of mechanical components in the valve and its actuation, facilitating its manufacture and use.
  • Using the teaching of the present invention it is possible to fabricate multilevel microfluidic systems where layers of microfluidic formations are separated by valving layers. As long as the laser-printed spots do not overlap, the appropriate valve can be selected on demand and formations on different layers connected at will.

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  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un dispositif fluidique (100) comprenant des première (120) et seconde parties (130) conformées séparées l'une de l'autre par un ou plusieurs séparateurs photoactivables (140) (141) localisés, les séparateurs photoactivables (140) (141) étant sensibles à la photoactivation pour passer d'un premier état fermé à un second état ouvert, l'adoption de l'état ouvert définissant un chemin fluidique entre chacune des première (120) et seconde parties (130) conformées. Le dispositif est de préférence rotatif, cette rotation sollicitant le déplacement du fluide d'une première partie conformée dans une seconde partie conformée.
PCT/EP2010/050801 2009-01-23 2010-01-25 Soupape fluidique à usage unique et systèmes microfluidiques intégrant ladite soupape WO2010084190A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0901115A GB2467298A (en) 2009-01-23 2009-01-23 Multilayer microfluidic device
GB0901115.6 2009-01-23
GB0902980.2 2009-02-23
GB0902980A GB2468111A (en) 2009-02-23 2009-02-23 Multilayer Fluidic Device

Publications (1)

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WO2010084190A1 true WO2010084190A1 (fr) 2010-07-29

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WO2012131556A1 (fr) 2011-03-24 2012-10-04 Biosurfit, S.A. Régulation d'une séquence d'écoulement de liquide sur un dispositif microfluidique
WO2012137122A1 (fr) 2011-04-02 2012-10-11 Biosurfit, S.A. Réserve de réactif liquide et fonctionnement de dispositifs analytiques
WO2014111721A1 (fr) * 2013-01-18 2014-07-24 The University Of Liverpool Plateforme microfluidique
WO2017066485A1 (fr) 2015-10-13 2017-04-20 Landers James P Dispositifs et procédés d'extraction, de séparation et de thermocyclage
GB2553100A (en) * 2016-08-19 2018-02-28 Univ Dublin City A microfluidic device

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US20070095393A1 (en) * 2004-03-30 2007-05-03 Piero Zucchelli Devices and methods for programmable microscale manipulation of fluids
EP2000210A1 (fr) * 2007-06-05 2008-12-10 Samsung Electronics Co., Ltd. Appareil microfluidique à réservoir de fluide
WO2009112030A1 (fr) * 2008-03-12 2009-09-17 Fluimedix Aps Manipulation de liquide régulée

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US5869002A (en) * 1996-02-12 1999-02-09 Bio Merieux Analysis card
US20020047003A1 (en) * 2000-06-28 2002-04-25 William Bedingham Enhanced sample processing devices, systems and methods
US20070095393A1 (en) * 2004-03-30 2007-05-03 Piero Zucchelli Devices and methods for programmable microscale manipulation of fluids
EP2000210A1 (fr) * 2007-06-05 2008-12-10 Samsung Electronics Co., Ltd. Appareil microfluidique à réservoir de fluide
WO2009112030A1 (fr) * 2008-03-12 2009-09-17 Fluimedix Aps Manipulation de liquide régulée

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012131556A1 (fr) 2011-03-24 2012-10-04 Biosurfit, S.A. Régulation d'une séquence d'écoulement de liquide sur un dispositif microfluidique
EP3000530A2 (fr) 2011-03-24 2016-03-30 Biosurfit, S.A. Séquence de régulation du débit de liquide sur un dispositif microfluidique
US9625916B2 (en) 2011-03-24 2017-04-18 Biosurfit, S.A. Control of liquid flow sequence on a microfluidic device
US9908116B2 (en) 2011-03-24 2018-03-06 Biosurfit, S.A. Control of liquid flow sequence on microfluidic device
WO2012137122A1 (fr) 2011-04-02 2012-10-11 Biosurfit, S.A. Réserve de réactif liquide et fonctionnement de dispositifs analytiques
EP3023335A1 (fr) 2011-04-02 2016-05-25 Biosurfit, S.A. Stockage de réactif liquide et fonctionnement de dispositifs analytiques
US9651460B2 (en) 2011-04-02 2017-05-16 Biosurfit, S.A. Liquid reagent storage and operation of analytical devices
US9983105B2 (en) 2011-04-02 2018-05-29 Biosurfit, S.A. Liquid reagent storage and operation of analytical devices
WO2014111721A1 (fr) * 2013-01-18 2014-07-24 The University Of Liverpool Plateforme microfluidique
WO2017066485A1 (fr) 2015-10-13 2017-04-20 Landers James P Dispositifs et procédés d'extraction, de séparation et de thermocyclage
GB2553100A (en) * 2016-08-19 2018-02-28 Univ Dublin City A microfluidic device

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