WO2010080978A3 - Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components - Google Patents
Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components Download PDFInfo
- Publication number
- WO2010080978A3 WO2010080978A3 PCT/US2010/020470 US2010020470W WO2010080978A3 WO 2010080978 A3 WO2010080978 A3 WO 2010080978A3 US 2010020470 W US2010020470 W US 2010020470W WO 2010080978 A3 WO2010080978 A3 WO 2010080978A3
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- WO
- WIPO (PCT)
- Prior art keywords
- whole blood
- leukocytes
- blood sample
- depletion
- capture
- Prior art date
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Zoology (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hospice & Palliative Care (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Methods, apparatuses, and microfluidic systems for pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components is described. A microfluidic device is used to pre-deplete the leukocytes in a whole blood sample. To do so, parallel micro-channels in the microfluidic device are treated with binding moieties to bind leukocytes but not target cells. When the whole blood sample is flowed through the microfluidic device, it is depleted of the leukocytes to form a depleted sample. The depleted sample is then flowed through a second micro-channel treated to bind target cells.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14331609P | 2009-01-08 | 2009-01-08 | |
US61/143,316 | 2009-01-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010080978A2 WO2010080978A2 (en) | 2010-07-15 |
WO2010080978A3 true WO2010080978A3 (en) | 2010-11-18 |
Family
ID=42317153
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2010/020470 WO2010080978A2 (en) | 2009-01-08 | 2010-01-08 | Pre-depletion of leukocytes in whole blood samples prior to the capture of whole blood sample components |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2010080978A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6991192B2 (en) | 2016-06-21 | 2022-01-12 | クァンタムディーエックス グループ リミテッド | Improving Target Cell Concentration Using Dielectrophoresis (DEP) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012024194A2 (en) * | 2010-08-15 | 2012-02-23 | Gpb Scientific, Llc | Microfluidic cell separation in the assay of blood |
EP2838636A4 (en) * | 2012-04-20 | 2015-12-16 | Agency Science Tech & Res | Microfilter and apparatus for separating a biological entity from a sample volume |
SG10201705895TA (en) * | 2013-01-24 | 2017-08-30 | Nat Univ Singapore | Microdevices for separation of non-spherical particles and applications thereof |
US10710077B2 (en) * | 2014-10-17 | 2020-07-14 | Ecole Polytechnique | Method for handling microdrops which include samples |
CN104483483A (en) * | 2014-12-22 | 2015-04-01 | 常熟市创新模具有限公司 | Device for detecting tumor cells |
CN104483482A (en) * | 2014-12-22 | 2015-04-01 | 常熟市创新模具有限公司 | Method for manufacturing device used for detecting tumor cells |
CN104560715B (en) * | 2015-01-31 | 2016-09-07 | 重庆大学 | Cultivate based on micro-fluidic chip and the method for detection lung carcinoma cell |
CN104560714B (en) * | 2015-01-31 | 2016-09-07 | 重庆大学 | For cultivating and detect the micro-fluidic chip of lung carcinoma cell |
US10774393B2 (en) | 2016-05-20 | 2020-09-15 | Roche Molecular Systems, Inc. | Cell surface marker depletion in a sample processing device |
CN105950469B (en) * | 2016-06-08 | 2018-03-06 | 牛海涛 | Cell screening chip and micro-fluidic chip joint |
CN106350439A (en) * | 2016-11-10 | 2017-01-25 | 上海美吉逾华生物医药科技有限公司 | Micro-fluidic chip for cell capture and fluorescent staining |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007106598A2 (en) * | 2006-03-15 | 2007-09-20 | The General Hospital Corporation | Devices and methods for detecting cells and other analytes |
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2010
- 2010-01-08 WO PCT/US2010/020470 patent/WO2010080978A2/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007106598A2 (en) * | 2006-03-15 | 2007-09-20 | The General Hospital Corporation | Devices and methods for detecting cells and other analytes |
Non-Patent Citations (3)
Title |
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ADAMS, A. A. ET AL.: "High Efficient Circulating Tumor Cell Isolation from Whole Blood and Label-Free Enumeration Using Polymer-Based Microfluidics with an Integrated Conductivity Sensor", J. AM. CHEM. SOC., vol. 130, no. 27, July 2008 (2008-07-01), pages 8633 - 8641 * |
NAGRATH, S. ET AL.: "Isolation of Rare Circulating Tumour Cells in Cancer Patients by Microchip Technology", NATURE. DEC., vol. 450, no. 7173, 2007, pages 1235 - 1239 * |
SOOHOO, J. ET AL.: "Microfluidic Liquid Filters for Leukocytes Isolation", CONF. PROC. IEEE ENG. MED. BIOL. SOC., 2007, pages 6319 - 6322 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6991192B2 (en) | 2016-06-21 | 2022-01-12 | クァンタムディーエックス グループ リミテッド | Improving Target Cell Concentration Using Dielectrophoresis (DEP) |
Also Published As
Publication number | Publication date |
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WO2010080978A2 (en) | 2010-07-15 |
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