WO2010008809A3 - Compositions and methods for early stage sex determination - Google Patents

Compositions and methods for early stage sex determination Download PDF

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Publication number
WO2010008809A3
WO2010008809A3 PCT/US2009/048182 US2009048182W WO2010008809A3 WO 2010008809 A3 WO2010008809 A3 WO 2010008809A3 US 2009048182 W US2009048182 W US 2009048182W WO 2010008809 A3 WO2010008809 A3 WO 2010008809A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
early stage
blood sample
sex determination
Prior art date
Application number
PCT/US2009/048182
Other languages
French (fr)
Other versions
WO2010008809A2 (en
Inventor
Christina Mork
Chinh Bach
Preston Al Baecker
Anna Vitebsky
Meng Hou
Original Assignee
Consumer Genetics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consumer Genetics, Inc. filed Critical Consumer Genetics, Inc.
Publication of WO2010008809A2 publication Critical patent/WO2010008809A2/en
Publication of WO2010008809A3 publication Critical patent/WO2010008809A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

This invention provides compositions and methods to determine the gender of a fetus at early stage gestation. The invention provides isolated oligonucleotides and compositions thereof complementary to a centromeric alphoid repeat sequence of the human Y chromosome, which can be used to amplify or detect endogenously cleaved male fetal DNA isolated from a maternal whole blood sample in an amplification assay. The invention also provide methods for detecting a paternally inherited nucleic acid of fetal origin in a maternal blood sample.
PCT/US2009/048182 2008-06-23 2009-06-22 Compositions and methods for early stage sex determination WO2010008809A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US7498608P 2008-06-23 2008-06-23
US61/074,986 2008-06-23
US11897808P 2008-12-01 2008-12-01
US61/118,978 2008-12-01

Publications (2)

Publication Number Publication Date
WO2010008809A2 WO2010008809A2 (en) 2010-01-21
WO2010008809A3 true WO2010008809A3 (en) 2010-03-25

Family

ID=41550947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/048182 WO2010008809A2 (en) 2008-06-23 2009-06-22 Compositions and methods for early stage sex determination

Country Status (1)

Country Link
WO (1) WO2010008809A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010051341A1 (en) * 1997-03-04 2001-12-13 Isis Innovation Limited Non-invasive prenatal diagnosis
US7074567B2 (en) * 2003-09-30 2006-07-11 Reliagene Technologies Inc. Assay for human DNA for gender determination

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010051341A1 (en) * 1997-03-04 2001-12-13 Isis Innovation Limited Non-invasive prenatal diagnosis
US7074567B2 (en) * 2003-09-30 2006-07-11 Reliagene Technologies Inc. Assay for human DNA for gender determination

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DAVE ET AL.: "Fetal cells in maternal blood for prenatal diagnosis", INDIAN J HUM GENET., vol. 5, no. 1, 1999, pages 1 - 14 *
HONDA ET AL.: "Fetal gender determination in early pregnancy through qualitative and quantitative analysis of fetal DNA in maternal serum", HUM GENET, vol. 110, 2002, pages 75 - 79 *
KOUPRIN ET AL.: "Cloning of human centromeres by transformation associated recombination in yeast and generation of functional human artificial chromosomes", NUCLEIC ACIDS RESEARCH, vol. 31, no. 3, 2003, pages 922 - 934 *
LIN ET AL.: "Sex determination by polymerase chain reaction on mummies discovered at Taklamakan desert in 1912", FORENSIC SCI INT., vol. 75, no. 2-3, 30 October 1995 (1995-10-30), pages 197 - 205 *
MURAKAMI ET AL.: "Forensic study of sex determination using PCR on teeth samples", ACTA MEDICA OKAYAMA, vol. 54, no. 1, 2000, pages 21 - 32 *
WITT ET AL.: "A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction", HUM GENET., vol. 82, no. 3, June 1989 (1989-06-01), pages 271 - 274 *

Also Published As

Publication number Publication date
WO2010008809A2 (en) 2010-01-21

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