WO2009149470A1 - Vcp-based vectors for algal cell transformation - Google Patents
Vcp-based vectors for algal cell transformation Download PDFInfo
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- WO2009149470A1 WO2009149470A1 PCT/US2009/046656 US2009046656W WO2009149470A1 WO 2009149470 A1 WO2009149470 A1 WO 2009149470A1 US 2009046656 W US2009046656 W US 2009046656W WO 2009149470 A1 WO2009149470 A1 WO 2009149470A1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8237—Externally regulated expression systems
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
Definitions
- This invention relates to molecular biology, and more specifically, to the expression of exogenous DNA elements in algal cells.
- Manipulating the DNA of a cell may confer upon the cell new abilities.
- the genetic manipulation is carried out by introducing functional DNA that was prepared outside the cell using molecular techniques.
- a transformed cell i.e., a cell that has taken-up exogenous DNA
- model biological systems i.e., well-studied organisms
- transformation is a major milestone that must be achieved to facilitate genetic engineering.
- Many algal species fall into the category of non-model organisms, with recalcitrant cell walls that make them notoriously difficult to transform. Accordingly, there is a need for an expression vectors for Nannochloropsis transformation.
- the vector comprises a Violaxanthin- chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell.
- Vcp Violaxanthin- chlorophyll a binding protein
- Embodiments of the invention may be used to introduce a gene (or genes) into the alga Nannochloropsis, such that the gene(s) are expressed and functional. This unprecedented ability to transform Nannochloropsis with high efficiency makes possible new developments in phycology, aquaculture and biofuels applications.
- FIG. IA shows a sequence of the genomic DNA of
- Nannochloropsis oceanica Nannochloropsis oceanica.
- FIG. IB shows an exemplary DNA transformation construct representing the functional insert of the PL90 vector.
- FIG. 2 shows an exemplary nucleotide sequence (SEQ. ID.
- FIG. 3 shows an exemplary nucleotide sequence (SEQ. ID. NO. 2) wherein the sh ble gene of the PL90 vector was replaced with a gene conferring resistance against hygromycin B.
- FIG. 4 shows an exemplary nucleotide sequence (SEQ. ID. NO. 3) wherein the sh ble gene of the PL90 vector was replaced with a gene conferring resistance against blastocidin.
- FIG. 5 shows the number of exemplary transformed algal cell colonies obtained when cells from a single transformation experiment have been plated under varying light conditions.
- FIG. 6 shows the number of exemplary transformed algal mutants obtained under varying zeocine concentrations.
- FIG. 7 shows the molecular analysis of exemplary transformed algal cells transformed with the PL90 vector and grown in the presence of zeocine.
- FIG. 8 shows an approximately 400 base pair fragment that indicates the exemplary linearized PL90 vector is stably integrated within the genome of Nannochlor ⁇ psis oceanica. DETAILED DESCRIPTION OF THE INVENTION
- Transformed algae may be useful in aquaculture production.
- the transformation of small algal cells with tough membranes, however, is difficult to achieve.
- Embodiments of the present invention are useful in the efficient transformation of Nannochloropsis, a microalga of about 3-5 micrometers in size.
- Vcp Violaxanthin-chlorophyll a binding protein
- Vcp promoter is active at lower light intensities, such that a transformation construct comprising a Vcp promoter may be useful in aquaculture ponds receiving less light, such as in the case of algae grown deep in a pond. Additionally, a Vcp promoter may be useful in modulating the expression of genes governed by the Vcp promoter, by varying the intensity of incident light.
- FIG. IA shows the sequence genomic DNA of Nannochloropsis oceanica, which includes the Vcp gene and regulatory elements. Please note that for illustration purposes, 2 exons and 1 intron within B3 are illustrated. In fact, the gene harbors more than these components.
- the sequenced genomic DNA has the structure A-B-C, where B is the DNA encoding the Vcp gene (including introns), A is the DNA sequence in front of the Vcp gene, and C is the DNA sequence after the Vcp gene.
- Sequence A includes the promoter which drives expression of the Vcp gene.
- the region from transcription start to translation start (the start ATG triplet) is the 5' -untranslated region A3.
- the sequence preceding the start methionine comprises Al, A2 and A3.
- the start methionine Bl is immediately followed by an intron B2 and the remaining exons and introns B3 of the Vcp gene.
- the Vcp gene ends with the stop codon B4.
- the sequence downstream of the Vcp gene (called C), includes the untranslated region Cl, a polyadenylation signal C2, the stop of transcription C3 and downstream DNA sequence C4.
- FIG. IB shows an exemplary DNA transformation construct representing the functional insert of the PL90 vector.
- part B3 (FIG. IA) was replaced with the reading frame of the sh ble gene found in Streptoalloteichus hindustanu, yielding the PL90 vector as described herein.
- FIG IB may also be used to show the structure of the various exemplary vector constructs PL90, H8 and B9 as described herein.
- the difference between the three exemplary vector constructs is the type of selection marker gene (SG) used: the sh ble gene (PL90), the hygromycin B phosphotransferase gene (H8), or the blastocidin S deaminase (B9) gene.
- SG selection marker gene
- Example One We identified a Vcp (violaxanthine chlorophyll a binding protein) gene in a public nucleotide database (NCBI) for a Nannochloropsis strain (http://www.ncbi.nlm.nih.gov/nuccore/2734863). We constructed primers against this gene and recovered the genomic area in front of and behind the gene. We designed a DNA transformation construct replacing part B3 of the genome (FIG. IA) with the reading frame of the sh ble gene from Streptoalloteichus hindustanus (which confers resistance against the drug bleomycine), yielding the exemplary PL90 vector. This exemplary construct is illustrated in FIG. IB.
- FIG. 2 shows an exemplary nucleotide sequence (SEQ. ID. NO. 1) for the insert of the PL90 vector.
- 202 represents A from FIGS. 1A-1B, which is the DNA sequence in front of the Vcp gene.
- 204 represents the left intron border of the first Vcp intron.
- 206 represents the start methionine of the Vcp gene.
- 208 represents the beginning of the selection marker gene (i.e., the beginning of the sh ble gene, ATG).
- 210 represents an introduced artificial sequence, TT. 212 represents the right intron border of the first Vcp intron.
- 214 represents the stop codon of the selection marker gene, TAA. 216 represents where polyadenylation occurs, after the CCGCCC sequence.
- 218 represents C from FIGS. 1 A-IB, which is the DNA sequence downstream of the Vcp gene.
- FIG. 3 shows an exemplary nucleotide sequence (SEQ. ID. NO. 2) wherein the sh ble gene of the PL90 vector was replaced with a gene conferring resistance against hygromycin B.
- 302 represents A from FIGS. IA- IB, which is the DNA sequence in front of the Vcp gene.
- 304 represents the left intron border of the first Vcp intron.
- 306 represents the start methionine of the Vcp gene.
- 308 represents the beginning of the selection marker gene (i.e., the beginning of the hygromycin B phosphotransferase gene, ATG).
- 310 represents an introduced artificial sequence, TT. 312 represents the right intron border of the first Vcp intron.
- 314 represents the stop codon of the selection marker gene, TAA.
- 316 represents where polyadenylation occurs, after the CCGCCC sequence.
- 318 represents C from FIGS. 1A-1B, which is the DNA sequence downstream of the Vcp
- FIG. 4 shows an exemplary nucleotide sequence (SEQ. ID. NO. 3) wherein the sh ble gene of the PL90 vector was replaced with a gene conferring resistance against blastocidin.
- 402 represents A from FIGS. 1 A-IB, which is the DNA sequence in front of the Vcp gene.
- 404 represents the left intron border of the first Vcp intron.
- 406 represents the start methionine of the Vcp gene.
- 408 represents the beginning of the selection marker gene (i.e., the beginning of the blasticidin-S deaminase gene, ATG).
- 410 represents an introduced artificial sequence
- TT. 412 represents the right intron border of the first Vcp intron.
- 414 represents the stop codon of the selection marker gene, TAA. 416 represents where polyadenylation occurs, after the CCGCCC sequence.
- 418 represents C from FIGS. 1A-1B, which is the DNA sequence downstream of the Vcp gene.
- the exemplary vectors PL90 (FIG. 2), H8 (FIG. 3) and B9 (FIG. 4) are useful for the transformation of Nannochloropsis. Selection occurred on 2 ⁇ g/ml zeocine (for vector PL90), 300 ⁇ g/ml hygromycin B (vector H8), or 5050 ⁇ g/ml blasticidin S (vector B9).
- Vcp promoter described herein drives expression of the Vcp of Nannochloropsis, a protein which is expressed in different levels at different physiological conditions. Algal cells acclimated to higher light intensities for example typically accumulate less light harvesting complexes than those acclimated to lower light intensities.
- Vcp promoter described herein confers resistance to higher concentrations of zeocine (thus indicating higher expression levels of the Vcp promoter- driven sh ble gene) in different light intensities.
- Nannochloropsis cells with the construct shown in FIG. 2 and allowed selection on agar plates in different light intensities.
- FIG. 5 shows the number of exemplary transformed algal cell colonies obtained when cells from a single transformation experiment have been plated under varying light conditions.
- FIG.5 shows that the number of colonies (which is equal to the number of transformed cells which can stand concentrations of zeocine as high as 25 ⁇ g/ml) increases with decreasing light intensities. The highest number of transformants was obtained at low light intensities at 5 ⁇ E ( ⁇ mol photons/(m2*s)). The result indicates that the exemplary construct utilized (as shown in FIG. 2) has a higher level of gene expression at lower light intensities than at higher light intensities. Accordingly, the exemplary constructs shown in FIGS. 2-4 might be utilized for the expression of genes modulated by the intensity of light. [0031] FIG.
- FIG. 6 shows the number of exemplary transformed algal mutants obtained and showing fifty percent (50%) or more growth at a given zeocine concentration but less than 50% at the next highest tested zeocine concentration.
- FIG. 6 illustrates the frequency of 96 clones obtained with the transformation vector PL90 showing more than 50% growth (in a liquid assay monitoring growth via OD750) at a certain zeocine concentration, but less than 50% at the next higher tested zeocine concentration.
- wild-type cells and control cells (those transformed with pjetl NOT containing a construct) never form colonies on zeocine concentrations 2 ⁇ g/ml or above, nor is there any detectable growth in liquid culture at such concentrations of zeocine.
- FIG. 7 shows the molecular analysis of exemplary transformed algal cells transformed with the PL90 vector and grown in the presence of zeocine. 12 randomly picked colonies were derived from a transformation event with the vector PL90 and selection on zeocine (2 ⁇ g/ml). A control colony was obtained from a plate with wild-type colonies. Cells were resuspended in buffer (Ix yellow tango buffer from Fermentas) and incubated with DNAse in order to digest possible residual extra cellular PL90 DNA used for the transformation event. The cells were then washed twice in seawater and resuspended in Millipore water and heated to 95 C in order to bring the intracellular DNA into solution.
- buffer Ix yellow tango buffer from Fermentas
- a standard PCR employing sh ble gene primers (113 BIe for short ATG GCC AAG TTG ACC AGT GCC GT, 111 BIe rev short TTA GTC CTG CTC CTC GGC CAC GAA) utilizing a taq polymerase was performed on lysates of the 12 colonies obtained after transformation (colonies 1-12), of the control wild type colony without (negative control NC) or with (positive control PC) vector PL90 added.
- the primers shown above correspond to the region on the pjetl vector right after the linearization restriction site. Note that the constructs PL90, H8 and B9 are within the vector pjetl. We recovered an approximately 400 base pair long fragment which we sequenced. The sequence is shown in FIG. 8.
- FIG. 8 shows an approximately 400 base pair fragment that indicates the exemplary linearized PL90 vector is stably integrated within the genome of Nannochloropsis oceanica.
- Nannochloropsis oceanica Nannochloropsis oceanica.
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EP09759628A EP2297326A4 (en) | 2008-06-06 | 2009-06-08 | Vcp-based vectors for algal cell transformation |
AU2009255947A AU2009255947B2 (en) | 2008-06-06 | 2009-06-08 | Vcp-based vectors for algal cell transformation |
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PCT/US2009/046650 WO2009149465A1 (en) | 2008-06-06 | 2009-06-08 | Transformation of algal cells |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6027900A (en) * | 1996-04-12 | 2000-02-22 | Carnegie Institution Of Washington | Methods and tools for transformation of eukaryotic algae |
US7244609B2 (en) * | 2001-03-09 | 2007-07-17 | Cayla | Synthetic genes and bacterial plasmids devoid of CpG |
Family Cites Families (92)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US1926780A (en) | 1931-11-11 | 1933-09-12 | John W Lippincott | Endless water course |
FR94705E (en) | 1966-06-01 | 1969-10-24 | Inst Francais Du Petrole | Improved method for cultivating algae and implementing device. |
US3962466A (en) | 1972-11-10 | 1976-06-08 | Dai-Nippon Sugar Manufacturing Co., Ltd. | Method for treatment of microorganisms |
US4003337A (en) | 1974-10-23 | 1977-01-18 | Kerry Lamar Moore | Fish growing tank and method |
US4267038A (en) | 1979-11-20 | 1981-05-12 | Thompson Worthington J | Controlled natural purification system for advanced wastewater treatment and protein conversion and recovery |
US4365938A (en) | 1980-01-14 | 1982-12-28 | Warinner Archie F | Modular low head high volume water pump and aquaculture system |
US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
US5668298A (en) * | 1984-12-24 | 1997-09-16 | Eli Lilly And Company | Selectable marker for development of vectors and transformation systems in plants |
US4658757A (en) | 1985-11-14 | 1987-04-21 | Ocean Ventures-1 | Method and apparatus for improved aquaculture/mariculture |
US5105085A (en) * | 1989-11-17 | 1992-04-14 | Mcguire Danny G | Fluid analysis system |
US7037692B1 (en) | 1990-03-16 | 2006-05-02 | Calgene, Inc. | Plant desaturases compositions and uses |
US5527456A (en) | 1992-06-02 | 1996-06-18 | Jensen; Kyle R. | Apparatus for water purification by culturing and harvesting attached algal communities |
US5661017A (en) * | 1993-09-14 | 1997-08-26 | Dunahay; Terri Goodman | Method to transform algae, materials therefor, and products produced thereby |
US5417550A (en) | 1993-11-02 | 1995-05-23 | Marine Gikens Co., Ltd. | Submersed jet pump for generating a stream of water |
ES2256856T3 (en) | 1995-04-06 | 2006-07-16 | Seminis Vegetable Seeds, Inc. | TRANSGENIC CELL SECTION PROCESS. |
JP2000503858A (en) | 1996-06-14 | 2000-04-04 | ラトガーズ・ザ・ステイト・ユニバーシティ・オブ・ニュージャージー | Plastid marker genes that can be selected based on editing |
US5823781A (en) * | 1996-07-29 | 1998-10-20 | Electronic Data Systems Coporation | Electronic mentor training system and method |
US6117313A (en) | 1996-12-27 | 2000-09-12 | Goldman; Joshua | Method and apparatus for aquaculture and for water treatment related thereto |
ATE435288T1 (en) | 1997-02-27 | 2009-07-15 | Suntory Holdings Ltd | DELTA-9 DESATURASE GENE |
ATE305048T1 (en) | 1997-08-01 | 2005-10-15 | Martek Biosciences Corp | NUTRIENT COMPOSITIONS CONTAINING DHA AND METHOD FOR THE PRODUCTION THEREOF |
US7834855B2 (en) * | 2004-08-25 | 2010-11-16 | Apple Inc. | Wide touchpad on a portable computer |
US6166231A (en) | 1998-12-15 | 2000-12-26 | Martek Biosciences Corporation | Two phase extraction of oil from biomass |
RU2336307C2 (en) | 2000-01-19 | 2008-10-20 | Мартек Биосайнсис Корпорейшн | Method of obtaining lipids (versions) and lipids obtained by this method |
US6831040B1 (en) * | 2000-01-27 | 2004-12-14 | The Regents Of The University Of California | Use of prolines for improving growth and other properties of plants and algae |
ATE312928T1 (en) | 2000-05-24 | 2005-12-15 | Dsm Ip Assets Bv | METHOD FOR PRODUCING ASTAXANTHIN |
WO2001098335A2 (en) * | 2000-06-20 | 2001-12-27 | Phycotransgenics, Llc | Transgenic algae for delivering antigens to an animal |
US20070178451A1 (en) | 2001-08-02 | 2007-08-02 | Molian Deng | Nucleic acid sequences from Chlorella sarokiniana and uses thereof |
DE10040814A1 (en) * | 2000-08-21 | 2002-03-07 | Thor Gmbh | Synergistic biocide composition |
US6871195B2 (en) | 2000-09-13 | 2005-03-22 | E-Promentor | Method and system for remote electronic monitoring and mentoring of computer assisted performance support |
EP1415160A2 (en) * | 2000-09-30 | 2004-05-06 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
US6723243B2 (en) * | 2001-04-19 | 2004-04-20 | Aquafiber Technologies Corporation | Periphyton filtration pre- and post-treatment system and method |
US7547551B2 (en) * | 2001-06-21 | 2009-06-16 | University Of Antwerp. | Transfection of eukaryontic cells with linear polynucleotides by electroporation |
US6736572B2 (en) | 2001-07-18 | 2004-05-18 | Brian Geraghty | Method and apparatus for reducing the pollution of boat harbors |
US7238477B2 (en) | 2001-09-24 | 2007-07-03 | Intel Corporation | Methods to increase nucleotide signals by Raman scattering |
US7381326B2 (en) | 2002-02-15 | 2008-06-03 | Israel Haddas | Mega flow system |
US7449568B2 (en) | 2002-02-28 | 2008-11-11 | Takara Bio Inc. | Alga-origin promoter, intron and terminator |
EP2266525B1 (en) | 2002-05-03 | 2012-07-11 | Martek Biosciences Corporation | High quality lipids and methods for producing by enzymatic liberation from biomass |
US20050260553A1 (en) | 2002-05-13 | 2005-11-24 | Isaac Berzin | Photobioreactor and process for biomass production and mitigation of pollutants in flue gases |
US20050064577A1 (en) | 2002-05-13 | 2005-03-24 | Isaac Berzin | Hydrogen production with photosynthetic organisms and from biomass derived therefrom |
US7821425B2 (en) | 2002-07-12 | 2010-10-26 | Atmel Corporation | Capacitive keyboard with non-locking reduced keying ambiguity |
WO2004071996A2 (en) * | 2003-02-10 | 2004-08-26 | Carlson Peter S | Carbon sequestration in aqueous environments |
BRPI0410538A (en) | 2003-05-27 | 2006-06-20 | Fmc Corp | method for aquatic vegetation control |
AU2003903453A0 (en) | 2003-07-07 | 2003-07-17 | The University Of Queensland | Production of hydrogen |
US20050095569A1 (en) | 2003-10-29 | 2005-05-05 | Patricia Franklin | Integrated multi-tiered simulation, mentoring and collaboration E-learning platform and its software |
KR100754687B1 (en) | 2003-12-12 | 2007-09-03 | 삼성전자주식회사 | Multi input device of wireless terminal and his control method |
US20050181345A1 (en) | 2003-12-19 | 2005-08-18 | Edumedia Development Corporation | Mentor based computer assisted learning |
ES2529572T3 (en) | 2004-04-22 | 2015-02-23 | Commonwealth Scientific And Industrial Research Organisation | Synthesis of long chain polyunsaturated fatty acids by recombinant cells |
CA2884237C (en) | 2004-04-22 | 2020-09-08 | Commonwealth Scientific And Industrial Research Organisation | Synthesis of long-chain polyunsaturated fatty acids by recombinant cells |
US20080194029A1 (en) | 2004-05-07 | 2008-08-14 | Peter Hegemann | Method for Increasing the Ratio of Homologous to Non-Homologous Recombination |
US20060031087A1 (en) | 2004-08-03 | 2006-02-09 | Fox Stephanie J | Mentor-protege matching system and method |
US7874808B2 (en) | 2004-08-26 | 2011-01-25 | Pentair Water Pool And Spa, Inc. | Variable speed pumping system and method |
US7402428B2 (en) | 2004-09-22 | 2008-07-22 | Arborgen, Llc | Modification of plant lignin content |
US7678931B2 (en) | 2004-10-22 | 2010-03-16 | Martek Biosciences Corporation | Process for preparing materials for extraction |
US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US20060155558A1 (en) * | 2005-01-11 | 2006-07-13 | Sbc Knowledge Ventures, L.P. | System and method of managing mentoring relationships |
KR100606803B1 (en) | 2005-05-16 | 2006-08-01 | 엘지전자 주식회사 | Mobile communication terminal with performing function using scroll wheel device and method of performing function using this |
US7770322B2 (en) | 2005-06-07 | 2010-08-10 | Hr Biopetroleum, Inc. | Continuous-batch hybrid process for production of oil and other useful products from photosynthetic microbes |
CN1956335B (en) | 2005-10-27 | 2010-06-23 | 盛群半导体股份有限公司 | Adjacent induction device and its induction method |
WO2007084078A1 (en) | 2006-04-22 | 2007-07-26 | Simlab Inventions & Consultancy Private Limited | A keyboard for a mobile phone or other portable communication devices |
US7745696B2 (en) | 2006-06-12 | 2010-06-29 | The Regents Of The University Of California | Suppression of Tla1 gene expression for improved solar conversion efficiency and photosynthetic productivity in plants and algae |
IL184971A0 (en) * | 2006-08-01 | 2008-12-29 | Brightsource Energy Inc | High density bioreactor system, devices and methods |
WO2008060571A2 (en) | 2006-11-13 | 2008-05-22 | Aurora Biofuels, Inc. | Methods and compositions for production and purification of biofuel from plants and microalgae |
US20080160488A1 (en) * | 2006-12-28 | 2008-07-03 | Medical Simulation Corporation | Trainee-as-mentor education and training system and method |
US9637714B2 (en) | 2006-12-28 | 2017-05-02 | Colorado State University Research Foundation | Diffuse light extended surface area water-supported photobioreactor |
BRPI0808442A2 (en) | 2007-03-07 | 2014-08-05 | Nventa Biopharmaceuticals Corp | CLOSED DOUBLE FILM NUCLEIC ACID COMPOSITIONS |
SG10201509864QA (en) * | 2007-06-01 | 2015-12-30 | Solazyme Inc | Production of oil in microorganisms |
US8993314B2 (en) | 2007-07-28 | 2015-03-31 | Ennesys Sas | Algae growth system for oil production |
WO2009018498A2 (en) | 2007-08-01 | 2009-02-05 | Bionavitas, Inc. | Illumination systems, devices, and methods for biomass production |
KR101442542B1 (en) | 2007-08-28 | 2014-09-19 | 엘지전자 주식회사 | Input device and portable terminal having the same |
US8598378B2 (en) | 2008-03-14 | 2013-12-03 | University Of Hawaii | Methods and compositions for extraction and transesterification of biomass components |
EP2271742A4 (en) * | 2008-03-31 | 2011-05-25 | Kuehnle Agrosystems Inc | Nuclear based expression of genes for production of biofuels and process co-products in algae |
US8119859B2 (en) | 2008-06-06 | 2012-02-21 | Aurora Algae, Inc. | Transformation of algal cells |
CN101289659B (en) | 2008-06-19 | 2010-12-22 | 中国海洋大学 | Delta6 fatty acid desaturated enzyme of marine microalgae and applications thereof |
US20090319338A1 (en) * | 2008-06-23 | 2009-12-24 | Parks Eric J | Method and system for virtual mentoring |
WO2010008490A1 (en) | 2008-06-25 | 2010-01-21 | Aurora Biofuels, Inc. | The use of 2-hydroxy-5-oxoproline in conjunction with algae |
US20100022393A1 (en) | 2008-07-24 | 2010-01-28 | Bertrand Vick | Glyphosate applications in aquaculture |
WO2010027455A1 (en) | 2008-09-04 | 2010-03-11 | Ciris Energy, Inc. | Solubilization of algae and algal materials |
US8170976B2 (en) * | 2008-10-17 | 2012-05-01 | The Boeing Company | Assessing student performance and providing instructional mentoring |
US20100198659A1 (en) | 2009-02-04 | 2010-08-05 | Sirota Consulting LLC | Methods for matching and managing mentors and mentees and systems thereof |
US8314228B2 (en) * | 2009-02-13 | 2012-11-20 | Aurora Algae, Inc. | Bidirectional promoters in Nannochloropsis |
WO2010094015A2 (en) | 2009-02-16 | 2010-08-19 | Advanced Lab Group Cooperative | System and related method for concentrating biological culture and circulating biological culture and process fluid |
US8809046B2 (en) | 2011-04-28 | 2014-08-19 | Aurora Algae, Inc. | Algal elongases |
US8865468B2 (en) | 2009-10-19 | 2014-10-21 | Aurora Algae, Inc. | Homologous recombination in an algal nuclear genome |
US9101942B2 (en) * | 2009-06-16 | 2015-08-11 | Aurora Algae, Inc. | Clarification of suspensions |
US20100323387A1 (en) | 2009-06-19 | 2010-12-23 | Shaun Bailey | Optimization of Response to Light |
US8404473B2 (en) | 2009-06-30 | 2013-03-26 | Aurora Algae, Inc. | Cyanobacterial isolates having auto-flocculation and settling properties |
US8709765B2 (en) * | 2009-07-20 | 2014-04-29 | Aurora Algae, Inc. | Manipulation of an alternative respiratory pathway in photo-autotrophs |
US20120107801A1 (en) | 2009-10-19 | 2012-05-03 | Oliver Kilian | High-efficiency homologous recombination in the oil-producing alga, nannochloropsis |
US8722359B2 (en) | 2011-01-21 | 2014-05-13 | Aurora Algae, Inc. | Genes for enhanced lipid metabolism for accumulation of lipids |
MX2013012565A (en) | 2011-04-28 | 2013-11-21 | Aurora Algae Inc | Algal desaturases. |
US8709766B2 (en) | 2011-10-17 | 2014-04-29 | Colorado School Of Mines | Use of endogenous promoters in genetic engineering of Nannochloropsis gaditana |
WO2013166065A1 (en) | 2012-04-30 | 2013-11-07 | Aurora Algae, Inc. | ACP Promoter |
-
2009
- 2009-06-08 US US12/480,611 patent/US8119859B2/en not_active Expired - Fee Related
- 2009-06-08 AU AU2009255947A patent/AU2009255947B2/en not_active Expired - Fee Related
- 2009-06-08 WO PCT/US2009/046656 patent/WO2009149470A1/en active Application Filing
- 2009-06-08 US US12/480,635 patent/US8318482B2/en not_active Expired - Fee Related
- 2009-06-08 EP EP09759628A patent/EP2297326A4/en not_active Ceased
- 2009-06-08 WO PCT/US2009/046650 patent/WO2009149465A1/en active Application Filing
-
2012
- 2012-02-13 US US13/372,467 patent/US8759615B2/en not_active Expired - Fee Related
- 2012-11-26 US US13/685,659 patent/US8685723B2/en not_active Expired - Fee Related
-
2013
- 2013-06-11 US US13/915,555 patent/US8753879B2/en not_active Expired - Fee Related
-
2014
- 2014-05-13 US US14/276,743 patent/US20150093830A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6027900A (en) * | 1996-04-12 | 2000-02-22 | Carnegie Institution Of Washington | Methods and tools for transformation of eukaryotic algae |
US7244609B2 (en) * | 2001-03-09 | 2007-07-17 | Cayla | Synthetic genes and bacterial plasmids devoid of CpG |
Non-Patent Citations (2)
Title |
---|
DATABASE NCBI 27 July 2006 (2006-07-27), XP008140573, Database accession no. EE109892 * |
SHI ET AL.: "Analysis of expressed sequence tags from the marine microalga Nannochloropsis oculata (eustigmatophyceae)", J PHYCOL, vol. 44, pages 99 - 102, XP008140572, Retrieved from the Internet <URL:http://www3.interscience.wiley.com/journal/119393460/abstract?CRETRY=1&SRETRY=0> * |
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WO2009149465A1 (en) | 2009-12-10 |
US8119859B2 (en) | 2012-02-21 |
AU2009255947A2 (en) | 2011-01-27 |
US20150093830A1 (en) | 2015-04-02 |
US20120208279A1 (en) | 2012-08-16 |
EP2297326A1 (en) | 2011-03-23 |
US20130295665A1 (en) | 2013-11-07 |
EP2297326A4 (en) | 2011-11-16 |
US8318482B2 (en) | 2012-11-27 |
US20130078716A1 (en) | 2013-03-28 |
WO2009149465A8 (en) | 2010-07-29 |
US20090317857A1 (en) | 2009-12-24 |
US8753879B2 (en) | 2014-06-17 |
AU2009255947A1 (en) | 2009-12-10 |
US20090317904A1 (en) | 2009-12-24 |
US8759615B2 (en) | 2014-06-24 |
AU2009255947B2 (en) | 2014-12-18 |
US8685723B2 (en) | 2014-04-01 |
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