WO2009116840A3 - Chemical reagents regulating stem cell fate - Google Patents

Chemical reagents regulating stem cell fate Download PDF

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Publication number
WO2009116840A3
WO2009116840A3 PCT/KR2009/001443 KR2009001443W WO2009116840A3 WO 2009116840 A3 WO2009116840 A3 WO 2009116840A3 KR 2009001443 W KR2009001443 W KR 2009001443W WO 2009116840 A3 WO2009116840 A3 WO 2009116840A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cell
chemical reagents
cell fate
present
regulating stem
Prior art date
Application number
PCT/KR2009/001443
Other languages
French (fr)
Other versions
WO2009116840A2 (en
Inventor
Dong Wook Kim
Ki-Chul Hwang
Sung Hou Kim
Ji Young Kim
Woo Chul Chang
Original Assignee
Industry-Academic Cooperation Foundation, Yonsei University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry-Academic Cooperation Foundation, Yonsei University filed Critical Industry-Academic Cooperation Foundation, Yonsei University
Publication of WO2009116840A2 publication Critical patent/WO2009116840A2/en
Publication of WO2009116840A3 publication Critical patent/WO2009116840A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention relates to a method for inducing differentiation of a stem cell, which comprises culturing the stem cell in the presence of an inhibitor to protein kinase A or Rho kinase. The present invention provides novel small molecules having isoquinolinesulfonamide scaffold to effectively induce differentiation of stem cells. The present invention allows for directed differentiation of stem cells to a specific cell type.
PCT/KR2009/001443 2008-03-21 2009-03-20 Chemical reagents regulating stem cell fate WO2009116840A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3842208P 2008-03-21 2008-03-21
US61/038,422 2008-03-21

Publications (2)

Publication Number Publication Date
WO2009116840A2 WO2009116840A2 (en) 2009-09-24
WO2009116840A3 true WO2009116840A3 (en) 2009-12-30

Family

ID=41091423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001443 WO2009116840A2 (en) 2008-03-21 2009-03-20 Chemical reagents regulating stem cell fate

Country Status (1)

Country Link
WO (1) WO2009116840A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011062013A1 (en) * 2009-11-18 2011-05-26 国立大学法人徳島大学 Inducer of selective differentiation into neural stem cells and/or neural precursor cells
WO2012036512A2 (en) * 2010-09-16 2012-03-22 연세대학교 산학협력단 Use of a compound for inducing differentiation of mesenchymal stem cells into cartilage cells
KR20120029225A (en) * 2010-09-16 2012-03-26 파미셀 주식회사 Use of ptk inhibitor for inducing differentiation of mesenchymal stem cells to cardiogenic cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEN, S. ET AL.: "Self renewal of embryonic stem cells by a small molecule.", PROC. NATL. ACAD. SCI. U. S. A., vol. 103, no. 46, 6 November 2006 (2006-11-06), pages 17266 - 17271 *
SAXE, J. P. ET AL.: "A phenotypic small-molecule screen identifies an orphan ligand-receptor pair that regulates neural stem cell differentiation.", CHEM. BIOL., vol. 14, no. 9, September 2007 (2007-09-01), pages 1019 - 1030 *
SCHUGAR, R. C. ET AL.: "Small molecules in stem cell self-renewal and differentiation.", GENE. THER., vol. 15, no. 2, 8 November 2007 (2007-11-08), pages 126 - 135 *
WATANABE, K. ET AL.: "A ROCK inhibitor permits survival of dissociated human embryonic stem cells.", NAT. BIOTECHNOL., vol. 25, no. 6, 27 May 2007 (2007-05-27), pages 681 - 686 *

Also Published As

Publication number Publication date
WO2009116840A2 (en) 2009-09-24

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