WO2009078438A1 - Microbe complex - Google Patents

Microbe complex Download PDF

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Publication number
WO2009078438A1
WO2009078438A1 PCT/JP2008/072979 JP2008072979W WO2009078438A1 WO 2009078438 A1 WO2009078438 A1 WO 2009078438A1 JP 2008072979 W JP2008072979 W JP 2008072979W WO 2009078438 A1 WO2009078438 A1 WO 2009078438A1
Authority
WO
WIPO (PCT)
Prior art keywords
microbe
complex
peptide
bind
starch
Prior art date
Application number
PCT/JP2008/072979
Other languages
French (fr)
Japanese (ja)
Inventor
Yoshio Katakura
Shirin Tarahomjoo
Suteaki Shioya
Original Assignee
Osaka University
Meiji Dairies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osaka University, Meiji Dairies Corporation filed Critical Osaka University
Priority to JP2009546283A priority Critical patent/JP5693849B2/en
Priority to CN200880121290.8A priority patent/CN102016003B/en
Publication of WO2009078438A1 publication Critical patent/WO2009078438A1/en
Priority to HK11108955.3A priority patent/HK1155200A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Provided is a means to deliver viable microbes to the intestinal tract by a simple method without genetically engineering the microbe. For this purpose, a complex (aggregate) of a microbe such as lactobacillus, a polysaccharide and a fusion peptide, wherein a peptide that has the ability to bind to the surface of the above microbe and a peptide that has the ability to bind to the above polysaccharide have been linked, for example, a fusion protein of the peptidoglycan-binding domain of peptidoglycan hydrolase and the starch-binding domain of amylase, is formed. Preferably, this complex is coated with amylose or starch.
PCT/JP2008/072979 2007-12-17 2008-12-17 Microbe complex WO2009078438A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009546283A JP5693849B2 (en) 2007-12-17 2008-12-17 Microbial complex
CN200880121290.8A CN102016003B (en) 2007-12-17 2008-12-17 Microbe complex
HK11108955.3A HK1155200A1 (en) 2007-12-17 2011-08-24 Microbe complex

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-325246 2007-12-17
JP2007325246 2007-12-17

Publications (1)

Publication Number Publication Date
WO2009078438A1 true WO2009078438A1 (en) 2009-06-25

Family

ID=40795551

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/072979 WO2009078438A1 (en) 2007-12-17 2008-12-17 Microbe complex

Country Status (5)

Country Link
JP (1) JP5693849B2 (en)
KR (1) KR20110007102A (en)
CN (1) CN102016003B (en)
HK (1) HK1155200A1 (en)
WO (1) WO2009078438A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2011021524A1 (en) * 2009-08-19 2013-01-24 国立大学法人名古屋大学 Peptides that induce adhesion of lactic acid bacteria and uses thereof

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101281226B1 (en) * 2011-08-18 2013-07-08 김호일 RedGingsen with lactobacillus coated micro-starch
CN113845584B (en) * 2021-11-08 2022-09-16 江苏三仪生物工程有限公司 Preparation method of recombinant avian epidermal growth factor

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU776069B2 (en) * 1999-11-08 2004-08-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Process and composition for preparing a lignocellulose-based product, and the product obtained by the process
KR100373104B1 (en) * 2000-04-06 2003-02-25 주식회사 바이오알앤즈 Coating method of microbe by microbial polysaccharide

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
"Abstracts of the 59th Annual Meeting of the Society for Biotechnology, Japan", August 2007, article TARAHOMJOO S ET AL.: "Construction of whole cell biocatalysts based on the cell surface adhesive enzymes.", pages: 89 *
"Conferring new functions on lactic acid bacteria with cell surface adhesive proteins", March 2008, DEPARTMENT OF BIOTECHNOLOGY, GRADUATE SCHOOL OF ENGINEERING, OSAKA UNIVERSITY, article TARAHOMJOO S: "A new strategy for enhancement of microbial viability in simulated gastric conditions based on the display of starch binding do...", pages: 43 - 56, 6... *
BOSMA T ET AL.: "Novel surface display system for proteins on non-genetically modified gram-positive bacteria.", APPL. ENVIRON. MICROBIOL., vol. 72, 2006, pages 880 - 889, XP002569980, DOI: doi:10.1128/AEM.72.1.880-889.2006 *
CRITTENDEN R ET AL.: "Adhesion of Bifidobacteria to granular starch and its implications in probiotic technologies.", APPL. ENVIRON. MICROBIOL., vol. 67, 2001, pages 3469 - 3475, XP002963187, DOI: doi:10.1128/AEM.67.8.3469-3475.2001 *
LEENHOUTS K ET AL.: "Anchoring of proteins to lactic acid bacteria.", ANTONIE VAN LEEUWENHOEK, vol. 76, 1999, pages 367 - 376, XP002493781, DOI: doi:10.1023/A:1002095802571 *
LIAN W-C ET AL.: "Viability of microencapsulated bifidobacteria in simulated gastric juice and bile solution.", INT. J. FOOD MICROBIOL., vol. 86, 2003, pages 293 - 301, XP002437472, DOI: doi:10.1016/S0168-1605(02)00563-9 *
RAHA A R ET AL.: "Cell surface display system for Lactococcus lactis: a novel development for oral vaccine.", APPL. MICROBIOL. BIOTECHNOL., vol. 68, 2005, pages 75 - 81, XP002374776, DOI: doi:10.1007/s00253-004-1851-8 *
RODRIGUEZ-SANOJA R ET AL.: "Microbial starch-binding domain.", CURR. OPIN. MICROBIOL., vol. 8, 2005, pages 260 - 267, XP004936708, DOI: doi:10.1016/j.mib.2005.04.013 *
TARAHOMJOO S ET AL.: "New strategy for enhancement of microbial viability in simulated gastric conditions based on display of starch-binding domain on cell surface.", J. BIOSCI. BIOENG., vol. 105, 3 June 2008 (2008-06-03), pages 503 - 507 *
YOSHIO KATAKURA ET AL.: "Denpun Ketsugo Domain Hyoso Teiji ni yoru Nyusankin eno Ieki Taisei no Fuyo", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2008 NENDO (HEISEI 20 NENDO) TAIKAI KOEN YOSHISHU, 5 March 2008 (2008-03-05), pages 130 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2011021524A1 (en) * 2009-08-19 2013-01-24 国立大学法人名古屋大学 Peptides that induce adhesion of lactic acid bacteria and uses thereof
JP5830744B2 (en) * 2009-08-19 2015-12-09 国立大学法人東京工業大学 Peptides that induce adhesion of lactic acid bacteria and uses thereof

Also Published As

Publication number Publication date
JPWO2009078438A1 (en) 2011-04-28
CN102016003B (en) 2014-01-01
CN102016003A (en) 2011-04-13
HK1155200A1 (en) 2012-05-11
JP5693849B2 (en) 2015-04-01
KR20110007102A (en) 2011-01-21

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