WO2009066919A3 - Gpt gene as a marker for selecting plant transformant - Google Patents

Gpt gene as a marker for selecting plant transformant Download PDF

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Publication number
WO2009066919A3
WO2009066919A3 PCT/KR2008/006784 KR2008006784W WO2009066919A3 WO 2009066919 A3 WO2009066919 A3 WO 2009066919A3 KR 2008006784 W KR2008006784 W KR 2008006784W WO 2009066919 A3 WO2009066919 A3 WO 2009066919A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant
vector
marker
gpt gene
gpt
Prior art date
Application number
PCT/KR2008/006784
Other languages
French (fr)
Other versions
WO2009066919A2 (en
Inventor
Jae Heung Jeon
Hyun Soon Kim
Yoon Sik Kim
Yong Hwa Lee
Mi Sun Kim
Sung Ran Min
Won Joong Jeong
Suk Yoon Kwon
Hyouk Joung
Original Assignee
Korea Res Inst Of Bioscience
Jae Heung Jeon
Hyun Soon Kim
Yoon Sik Kim
Yong Hwa Lee
Mi Sun Kim
Sung Ran Min
Won Joong Jeong
Suk Yoon Kwon
Hyouk Joung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Res Inst Of Bioscience, Jae Heung Jeon, Hyun Soon Kim, Yoon Sik Kim, Yong Hwa Lee, Mi Sun Kim, Sung Ran Min, Won Joong Jeong, Suk Yoon Kwon, Hyouk Joung filed Critical Korea Res Inst Of Bioscience
Publication of WO2009066919A2 publication Critical patent/WO2009066919A2/en
Publication of WO2009066919A3 publication Critical patent/WO2009066919A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/827—Solanum tuberosum [potato]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a marker consisting of GPT (UDP-Λ/-acetylgluco samine:dolichol phosphate-Λ/-acetylglucosamine-phosphotransferase) gene to select a transformed plant, a recombinant plant expression vector comprising GPT gene, plants that are transformed with said vector and seeds of the plants, a method of selecting a tr ansformed plant comprising steps of transforming a plant cell with said vector and selec ting a transformant in a medium comprising tunicamycin, and a method of producing a t ransformed plant comprising a step of transforming a plant cell with said vector.
PCT/KR2008/006784 2007-11-23 2008-11-18 Gpt gene as a marker for selecting plant transformant WO2009066919A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070119959A KR100927803B1 (en) 2007-11-23 2007-11-23 JPPT gene as a marker for selecting plant transformants
KR10-2007-0119959 2007-11-23

Publications (2)

Publication Number Publication Date
WO2009066919A2 WO2009066919A2 (en) 2009-05-28
WO2009066919A3 true WO2009066919A3 (en) 2009-08-06

Family

ID=40667979

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/006784 WO2009066919A2 (en) 2007-11-23 2008-11-18 Gpt gene as a marker for selecting plant transformant

Country Status (2)

Country Link
KR (1) KR100927803B1 (en)
WO (1) WO2009066919A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102333A1 (en) 2014-08-19 2017-02-22 Regeneron Pharma EFFICIENT SELECTIVITY OF RECOMBINED PROTEINS

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRIAN MIKI ET AL.: "Selectable marker genes in transgenic plants: applications, alternatives and biosafety", JOURNAL OF BIOTECHNOLOGY, vol. 107, 2004, pages 193 - 232 *
DATABASE GENEBANK NOZOMU KOIZUMI ET AL.: "Overexpression of a Gene That Encodes the First Enzyme in the Biosynthesis of Asparagine-Linked Glycans Makes Plants Resistant to Tunicamycin and Obviates the Tunicamycin-Induced Unfolded Protein Response", Database accession no. D88036 *
NOZOMU KOIZUMI: "A chimeric tunicamycin resistant gene as a new selectable marker for Arabidopsis thaliana", PLANT BIOTECHNOLOGY, vol. 20, no. 4, 2003, pages 305 - 309 *
PLANT PHYSIOLOGY, vol. 121, October 1999 (1999-10-01), pages 353 - 361 *

Also Published As

Publication number Publication date
KR20090053230A (en) 2009-05-27
WO2009066919A2 (en) 2009-05-28
KR100927803B1 (en) 2009-11-23

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