WO2009054226A1 - たんぱく質の精製方法 - Google Patents

たんぱく質の精製方法 Download PDF

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Publication number
WO2009054226A1
WO2009054226A1 PCT/JP2008/067540 JP2008067540W WO2009054226A1 WO 2009054226 A1 WO2009054226 A1 WO 2009054226A1 JP 2008067540 W JP2008067540 W JP 2008067540W WO 2009054226 A1 WO2009054226 A1 WO 2009054226A1
Authority
WO
WIPO (PCT)
Prior art keywords
purification method
protein purification
mixed solution
graft chain
impurities
Prior art date
Application number
PCT/JP2008/067540
Other languages
English (en)
French (fr)
Inventor
Hironobu Shirataki
Naoyuki Shinohara
Original Assignee
Asahi Kasei Chemicals Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Kasei Chemicals Corporation filed Critical Asahi Kasei Chemicals Corporation
Priority to EP08842007A priority Critical patent/EP2226331A4/en
Priority to CN200880113118A priority patent/CN101835791A/zh
Priority to AU2008315124A priority patent/AU2008315124B2/en
Priority to US12/681,189 priority patent/US8653246B2/en
Priority to EP14150758.2A priority patent/EP2719705B1/en
Priority to KR1020107003536A priority patent/KR101163307B1/ko
Priority to JP2009538030A priority patent/JP5778389B2/ja
Priority to CA2703086A priority patent/CA2703086C/en
Publication of WO2009054226A1 publication Critical patent/WO2009054226A1/ja
Priority to US13/572,983 priority patent/US20120322983A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/08—Hollow fibre membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B01D71/261—Polyethylene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/76—Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74
    • B01D71/78—Graft polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/76—Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74
    • B01D71/82—Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74 characterised by the presence of specified groups, e.g. introduced by chemical after-treatment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/30—Polyalkenyl halides
    • B01D71/32—Polyalkenyl halides containing fluorine atoms
    • B01D71/34—Polyvinylidene fluoride

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)

Abstract

　本発明は、目的とするたんぱく質と不純物とを含む混合液から前記不純物を除去するためのたんぱく質の精製方法であって、細孔表面にグラフト鎖を有し、かつ前記グラフト鎖にアニオン交換基が固定される多孔膜を用いてろ過する工程を含む、たんぱく質の精製方法を提供する。
PCT/JP2008/067540 2007-10-26 2008-09-26 たんぱく質の精製方法 WO2009054226A1 (ja)

Priority Applications (9)

Application Number Priority Date Filing Date Title
EP08842007A EP2226331A4 (en) 2007-10-26 2008-09-26 METHOD OF PURIFYING PROTEINS
CN200880113118A CN101835791A (zh) 2007-10-26 2008-09-26 蛋白质的提纯方法
AU2008315124A AU2008315124B2 (en) 2007-10-26 2008-09-26 Method for purifying protein
US12/681,189 US8653246B2 (en) 2007-10-26 2008-09-26 Method for purifying protein
EP14150758.2A EP2719705B1 (en) 2007-10-26 2008-09-26 Method for purifying protein
KR1020107003536A KR101163307B1 (ko) 2007-10-26 2008-09-26 단백질의 정제 방법
JP2009538030A JP5778389B2 (ja) 2007-10-26 2008-09-26 たんぱく質の精製方法
CA2703086A CA2703086C (en) 2007-10-26 2008-09-26 Method for purifying protein
US13/572,983 US20120322983A1 (en) 2007-10-26 2012-08-13 Method for purifying protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-279406 2007-10-26
JP2007279406 2007-10-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/572,983 Division US20120322983A1 (en) 2007-10-26 2012-08-13 Method for purifying protein

Publications (1)

Publication Number Publication Date
WO2009054226A1 true WO2009054226A1 (ja) 2009-04-30

Family

ID=40579333

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/067540 WO2009054226A1 (ja) 2007-10-26 2008-09-26 たんぱく質の精製方法

Country Status (9)

Country Link
US (2) US8653246B2 (ja)
EP (2) EP2226331A4 (ja)
JP (3) JP5778389B2 (ja)
KR (1) KR101163307B1 (ja)
CN (1) CN101835791A (ja)
AU (1) AU2008315124B2 (ja)
CA (1) CA2703086C (ja)
TW (2) TW201350183A (ja)
WO (1) WO2009054226A1 (ja)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010241761A (ja) * 2009-04-09 2010-10-28 Asahi Kasei Chemicals Corp アニオン交換基が固定された多孔膜を用いた抗体モノマーの精製方法
WO2011001963A1 (ja) * 2009-07-03 2011-01-06 旭化成ケミカルズ株式会社 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法
JP2012116828A (ja) * 2011-07-05 2012-06-21 Asahi Kasei Medical Co Ltd タンパク質の精製方法
WO2012091070A1 (ja) 2010-12-27 2012-07-05 旭化成ケミカルズ株式会社 吸着分離膜モジュール、吸着分離膜モジュールの製造方法、及び仕切部材
JP2012211110A (ja) * 2011-03-31 2012-11-01 Asahi Kasei Medical Co Ltd 多孔質吸着膜
JP2012214408A (ja) * 2011-03-31 2012-11-08 Asahi Kasei Medical Co Ltd 澄明液中に分散した不純物凝集体を除去することによるタンパク質の精製方法
JP2012224729A (ja) * 2011-04-19 2012-11-15 Asahi Kasei Medical Co Ltd グラフト鎖を導入する方法、多孔質吸着膜、及びタンパク質の精製方法
WO2013047731A1 (ja) 2011-09-30 2013-04-04 旭化成ケミカルズ株式会社 タンパク質の精製方法
WO2013089141A1 (ja) 2011-12-15 2013-06-20 旭化成ケミカルズ株式会社 タンパク質吸着材
JP2013529931A (ja) * 2010-07-02 2013-07-25 レンブラント・エンタープライジーズ・インコーポレイテッド 単離された卵タンパク質及び卵脂質材料、並びにそれらを製造するための方法
US8653246B2 (en) 2007-10-26 2014-02-18 Asahi Kasei Chemicals Corporation Method for purifying protein
JP2015503524A (ja) * 2011-12-22 2015-02-02 ジェネンテック， インコーポレイテッド イオン交換膜クロマトグラフィー
JP2015167906A (ja) * 2014-03-06 2015-09-28 富士通株式会社 金属イオン除去装置及び液冷装置
JP2016531558A (ja) * 2013-09-30 2016-10-13 クルセル ホランド ベー ヴェー 高密度粗製細胞培養回収物の清澄化のための方法
JP2017025025A (ja) * 2015-07-23 2017-02-02 旭化成メディカル株式会社 タンパク質の精製方法
CN110563796A (zh) * 2019-10-12 2019-12-13 西藏天虹科技股份有限责任公司 青稞蛋白质的分离纯化方法

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WO2014003176A1 (ja) * 2012-06-29 2014-01-03 旭化成メディカル株式会社 プロテインａのｂドメイン変異体を含むポリペプチドが結合された担体からなる吸着材
WO2014126575A1 (en) 2013-02-14 2014-08-21 Nanofiber Separations, Llc Multi-chip module with a compressible structure for maintaining alignment between chips in the module
JPWO2017126496A1 (ja) * 2016-01-22 2018-08-23 旭化成メディカル株式会社 タンパク質の精製方法
WO2018087339A1 (en) 2016-11-11 2018-05-17 Ge Healthcare Bio-Sciences Ab Chromatography column
EP3495376A1 (en) * 2017-12-05 2019-06-12 ChromaCon AG Improved chromatographic process for cell culture harvest
GB201815405D0 (en) * 2018-09-21 2018-11-07 Fujifilm Mfg Europe Bv Membranes suitable for detecting, filtering and/or purifying biomolecules
US11585016B2 (en) * 2021-05-21 2023-02-21 Cambridge Crops, Inc. Systems and methods for manufacturing a silk fibroin solution and powders containing silk fibroin

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Cited By (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8653246B2 (en) 2007-10-26 2014-02-18 Asahi Kasei Chemicals Corporation Method for purifying protein
JP2010241761A (ja) * 2009-04-09 2010-10-28 Asahi Kasei Chemicals Corp アニオン交換基が固定された多孔膜を用いた抗体モノマーの精製方法
JPWO2011001963A1 (ja) * 2009-07-03 2012-12-13 旭化成ケミカルズ株式会社 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法
WO2011001963A1 (ja) * 2009-07-03 2011-01-06 旭化成ケミカルズ株式会社 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法
US9441011B2 (en) 2009-07-03 2016-09-13 Asahi Kasei Chemicals Corporation Method for purification of antibody using porous membrane having amino group and alkyl group both bound to graft chain immobilized on porous substrate
JP2016128446A (ja) * 2010-07-02 2016-07-14 レンブラント・エンタープライジーズ・インコーポレイテッドＲｅｍｂｒａｎｄｔ Ｅｎｔｅｒｐｒｉｓｅｓ，Ｉｎｃ． 単離された卵タンパク質及び卵脂質材料、並びにそれらを製造するための方法
JP2013529931A (ja) * 2010-07-02 2013-07-25 レンブラント・エンタープライジーズ・インコーポレイテッド 単離された卵タンパク質及び卵脂質材料、並びにそれらを製造するための方法
WO2012091070A1 (ja) 2010-12-27 2012-07-05 旭化成ケミカルズ株式会社 吸着分離膜モジュール、吸着分離膜モジュールの製造方法、及び仕切部材
JP2012214408A (ja) * 2011-03-31 2012-11-08 Asahi Kasei Medical Co Ltd 澄明液中に分散した不純物凝集体を除去することによるタンパク質の精製方法
JP2012211110A (ja) * 2011-03-31 2012-11-01 Asahi Kasei Medical Co Ltd 多孔質吸着膜
JP2012224729A (ja) * 2011-04-19 2012-11-15 Asahi Kasei Medical Co Ltd グラフト鎖を導入する方法、多孔質吸着膜、及びタンパク質の精製方法
JP2012116828A (ja) * 2011-07-05 2012-06-21 Asahi Kasei Medical Co Ltd タンパク質の精製方法
WO2013047731A1 (ja) 2011-09-30 2013-04-04 旭化成ケミカルズ株式会社 タンパク質の精製方法
US20140235837A1 (en) * 2011-09-30 2014-08-21 Asahi Kasei Chemicals Corporation Method for purifying protein
US9428544B2 (en) 2011-09-30 2016-08-30 Asahi Kasei Chemicals Corporation Method for purifying protein
US9545627B2 (en) 2011-12-15 2017-01-17 Asahi Kasei Chemicals Corporation Protein adsorbent
US20140357742A1 (en) * 2011-12-15 2014-12-04 Asahi Kasei Chemicals Corporation Protein adsorbent
JPWO2013089141A1 (ja) * 2011-12-15 2015-04-27 旭化成ケミカルズ株式会社 タンパク質吸着材
WO2013089141A1 (ja) 2011-12-15 2013-06-20 旭化成ケミカルズ株式会社 タンパク質吸着材
JP2020040952A (ja) * 2011-12-22 2020-03-19 ジェネンテック， インコーポレイテッド イオン交換膜クロマトグラフィー
JP2015503524A (ja) * 2011-12-22 2015-02-02 ジェネンテック， インコーポレイテッド イオン交換膜クロマトグラフィー
US10364268B2 (en) 2011-12-22 2019-07-30 Genentech, Inc. Ion exchange membrane chromatography
JP2021169454A (ja) * 2011-12-22 2021-10-28 ジェネンテック， インコーポレイテッド イオン交換膜クロマトグラフィー
JP7359807B2 (ja) 2011-12-22 2023-10-11 ジェネンテック， インコーポレイテッド イオン交換膜クロマトグラフィー
US11945837B2 (en) 2011-12-22 2024-04-02 Genentech, Inc. Ion exchange membrane chromatography
JP2016531558A (ja) * 2013-09-30 2016-10-13 クルセル ホランド ベー ヴェー 高密度粗製細胞培養回収物の清澄化のための方法
JP2015167906A (ja) * 2014-03-06 2015-09-28 富士通株式会社 金属イオン除去装置及び液冷装置
JP2017025025A (ja) * 2015-07-23 2017-02-02 旭化成メディカル株式会社 タンパク質の精製方法
CN110563796A (zh) * 2019-10-12 2019-12-13 西藏天虹科技股份有限责任公司 青稞蛋白质的分离纯化方法
CN110563796B (zh) * 2019-10-12 2023-05-12 西藏天虹科技股份有限责任公司 青稞蛋白质的分离纯化方法

Also Published As

Publication number Publication date
EP2719705B1 (en) 2020-07-29
JP5777659B2 (ja) 2015-09-09
AU2008315124B2 (en) 2012-06-21
JP2013173789A (ja) 2013-09-05
CA2703086C (en) 2014-11-25
KR101163307B1 (ko) 2012-07-05
JPWO2009054226A1 (ja) 2011-03-03
US20100228010A1 (en) 2010-09-09
US8653246B2 (en) 2014-02-18
AU2008315124A1 (en) 2009-04-30
EP2719705A1 (en) 2014-04-16
KR20100043243A (ko) 2010-04-28
TWI412394B (zh) 2013-10-21
JP5778389B2 (ja) 2015-09-16
TW201350183A (zh) 2013-12-16
JP2013163686A (ja) 2013-08-22
CA2703086A1 (en) 2009-04-30
CN101835791A (zh) 2010-09-15
EP2226331A4 (en) 2012-02-08
EP2226331A1 (en) 2010-09-08
US20120322983A1 (en) 2012-12-20
TW200932330A (en) 2009-08-01

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