WO2009045469A3 - Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof - Google Patents

Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof Download PDF

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Publication number
WO2009045469A3
WO2009045469A3 PCT/US2008/011409 US2008011409W WO2009045469A3 WO 2009045469 A3 WO2009045469 A3 WO 2009045469A3 US 2008011409 W US2008011409 W US 2008011409W WO 2009045469 A3 WO2009045469 A3 WO 2009045469A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acids
precursors
rna
micro
increasing
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Application number
PCT/US2008/011409
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French (fr)
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WO2009045469A2 (en
Inventor
Sumedha D Jayasena
Susan Swift
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Amgen Inc
Sumedha D Jayasena
Susan Swift
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Publication date
Application filed by Amgen Inc, Sumedha D Jayasena, Susan Swift filed Critical Amgen Inc
Priority to EP08836078A priority Critical patent/EP2205741A2/en
Priority to CA2700953A priority patent/CA2700953A1/en
Priority to MX2010003465A priority patent/MX2010003465A/en
Priority to AU2008307482A priority patent/AU2008307482B2/en
Priority to US12/681,160 priority patent/US8470794B2/en
Priority to JP2010527987A priority patent/JP2010539978A/en
Publication of WO2009045469A2 publication Critical patent/WO2009045469A2/en
Publication of WO2009045469A3 publication Critical patent/WO2009045469A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/317—Chemical structure of the backbone with an inverted bond, e.g. a cap structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA

Abstract

Methods and compositions relating to nucleic acids targeting certain miRNA molecules are disclosed. The nucleic acids are useful, for example, in methods of increasing the expression and/or secretion of EPO and treating various disease states including anemia, hemophilia, and/or sickle cell disease.
PCT/US2008/011409 2007-01-31 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof WO2009045469A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP08836078A EP2205741A2 (en) 2007-10-02 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof
CA2700953A CA2700953A1 (en) 2007-10-02 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof
MX2010003465A MX2010003465A (en) 2007-10-02 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof.
AU2008307482A AU2008307482B2 (en) 2007-10-02 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-RNA and precursors thereof
US12/681,160 US8470794B2 (en) 2007-01-31 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-RNA and precursors thereof
JP2010527987A JP2010539978A (en) 2007-10-02 2008-10-01 Increased erythropoietin using nucleic acids that can hybridize to micro-RNA and its precursors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97701707P 2007-10-02 2007-10-02
US60/977,017 2007-10-02

Publications (2)

Publication Number Publication Date
WO2009045469A2 WO2009045469A2 (en) 2009-04-09
WO2009045469A3 true WO2009045469A3 (en) 2009-06-04

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/011409 WO2009045469A2 (en) 2007-01-31 2008-10-01 Increasing erythropoietin using nucleic acids hybridizable to micro-rna and precursors thereof

Country Status (6)

Country Link
EP (1) EP2205741A2 (en)
JP (2) JP2010539978A (en)
AU (1) AU2008307482B2 (en)
CA (1) CA2700953A1 (en)
MX (1) MX2010003465A (en)
WO (1) WO2009045469A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2011005910A (en) * 2008-12-04 2011-06-17 Opko Curna Llc Treatment of erythropoietin (epo) related diseases by inhibition of natural antisense transcript to epo.
EP2327783A1 (en) * 2009-11-27 2011-06-01 Universitätsklinikum Freiburg Pharmaceutical composition comprising miRNA-100 and its use in the modulation of blood vessel growth
WO2011157294A1 (en) * 2010-06-16 2011-12-22 Universita' Degli Studi Di Padova Compositions for use in treating or preventing cancer, breast cancer, lung cancer, ovarian cancer, metastasis, heart failure, cardiac remodelling, dilated cardiomyopathy, autoimmune diseases, or diseases or disorders related thereto
CA2804599C (en) 2010-07-06 2023-01-31 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003053997A2 (en) * 2001-12-06 2003-07-03 Fibrogen, Inc. Methods of increasing endogenous erythropoietin (epo)

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