WO2009028644A1 - 偏性嫌気性細菌の検出用プローブおよびプライマー - Google Patents

偏性嫌気性細菌の検出用プローブおよびプライマー Download PDF

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Publication number
WO2009028644A1
WO2009028644A1 PCT/JP2008/065495 JP2008065495W WO2009028644A1 WO 2009028644 A1 WO2009028644 A1 WO 2009028644A1 JP 2008065495 W JP2008065495 W JP 2008065495W WO 2009028644 A1 WO2009028644 A1 WO 2009028644A1
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WIPO (PCT)
Prior art keywords
probe
primer
detection
obligate anaerobic
anaerobic bacteria
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PCT/JP2008/065495
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English (en)
French (fr)
Inventor
Nobuyuki Hayashi
Original Assignee
Kirin Beer Kabushiki Kaisha
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Application filed by Kirin Beer Kabushiki Kaisha filed Critical Kirin Beer Kabushiki Kaisha
Priority to JP2009530193A priority Critical patent/JPWO2009028644A1/ja
Publication of WO2009028644A1 publication Critical patent/WO2009028644A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明は、偏性嫌気性細菌を、正確、迅速、かつ簡便に識別できるプローブおよびプライマーの提供をその目的とする。本発明によれば、配列番号１～５、７～１１、１３～１６、１８～２２、および２４～２７の塩基配列で表されるポリヌクレオチドまたはその相同ポリヌクレオチドからなる群から選択されるプライマーを含んでなる、偏性嫌気性細菌の検出に用いられるＬＡＭＰ法プライマーセットが提供される。
PCT/JP2008/065495 2007-08-30 2008-08-29 偏性嫌気性細菌の検出用プローブおよびプライマー WO2009028644A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009530193A JPWO2009028644A1 (ja) 2007-08-30 2008-08-29 偏性嫌気性細菌の検出用プローブおよびプライマー

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-223783 2007-08-30
JP2007223783 2007-08-30

Publications (1)

Publication Number Publication Date
WO2009028644A1 true WO2009028644A1 (ja) 2009-03-05

Family

ID=40387355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065495 WO2009028644A1 (ja) 2007-08-30 2008-08-29 偏性嫌気性細菌の検出用プローブおよびプライマー

Country Status (2)

Country Link
JP (1) JPWO2009028644A1 (ja)
WO (1) WO2009028644A1 (ja)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003250557A (ja) * 2002-02-28 2003-09-09 Sapporo Breweries Ltd ジャイレース遺伝子を用いてラクトバチルス・ブレビス菌のビール混濁性を判定する方法
WO2005093059A1 (ja) * 2004-03-25 2005-10-06 Sapporo Breweries Limited 酵母、乳酸菌及び偏性嫌気性菌検出・識別のためのプライマー及びプライマーセット並びにそれらを用いた検出・識別方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003250557A (ja) * 2002-02-28 2003-09-09 Sapporo Breweries Ltd ジャイレース遺伝子を用いてラクトバチルス・ブレビス菌のビール混濁性を判定する方法
WO2005093059A1 (ja) * 2004-03-25 2005-10-06 Sapporo Breweries Limited 酵母、乳酸菌及び偏性嫌気性菌検出・識別のためのプライマー及びプライマーセット並びにそれらを用いた検出・識別方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TOMOO OGATA: "Beer jozo kankei biseibutsu no atarashii keitogakuteki chiken to kore o riyo shita yugai biseibutsu kenshutsuho", JOURNAL OF THE BREWING SOCIETY OF JAPAN, vol. 95, 2000, pages 803 - 810 *
YAMAMOTO S. & HARAYAMA S.: "PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains", APPL.ENVIRON.MICROBIOL., vol. 61, 1995, pages 1104 - 1109 *

Also Published As

Publication number Publication date
JPWO2009028644A1 (ja) 2010-12-02

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