WO2009027708A3 - Pulsed electrochemical sensor - Google Patents

Pulsed electrochemical sensor Download PDF

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Publication number
WO2009027708A3
WO2009027708A3 PCT/GB2008/002952 GB2008002952W WO2009027708A3 WO 2009027708 A3 WO2009027708 A3 WO 2009027708A3 GB 2008002952 W GB2008002952 W GB 2008002952W WO 2009027708 A3 WO2009027708 A3 WO 2009027708A3
Authority
WO
WIPO (PCT)
Prior art keywords
sensor
affinity agent
affinity
allowing
electrochemical sensor
Prior art date
Application number
PCT/GB2008/002952
Other languages
French (fr)
Other versions
WO2009027708A2 (en
Inventor
Timothy David Gibson
John Griffiths
Daniel James Lonsdale
Original Assignee
Elisha Systems Ltd
Timothy David Gibson
John Griffiths
Daniel James Lonsdale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elisha Systems Ltd, Timothy David Gibson, John Griffiths, Daniel James Lonsdale filed Critical Elisha Systems Ltd
Publication of WO2009027708A2 publication Critical patent/WO2009027708A2/en
Publication of WO2009027708A3 publication Critical patent/WO2009027708A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of detecting a biological affinity agent, comprising the steps of: providing a sensor comprising an antibody or other affinity reagent immobilised on a conductive substrate; the sensor further comprising one or more working electrodes, a counter- electrode and a reference electrode; contacting the sensor with an analyte containing the affinity agent; allowing the affinity agent to bind with the reagent; applying a voltage pulse to the sensor to polarise the sensor; disconnecting the voltage from the sensor and allowing the sensor to equilibrate in open circuit; measuring the rate of potential relaxation at the sensor; and using the measured rate of relaxation to calculate the amount of the affinity agent.
PCT/GB2008/002952 2007-08-30 2008-08-29 Pulsed electrochemical sensor WO2009027708A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0716877A GB0716877D0 (en) 2007-08-30 2007-08-30 Pulsed electrochemical sensor
GB0716877.6 2007-08-30

Publications (2)

Publication Number Publication Date
WO2009027708A2 WO2009027708A2 (en) 2009-03-05
WO2009027708A3 true WO2009027708A3 (en) 2009-04-23

Family

ID=38617002

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/002952 WO2009027708A2 (en) 2007-08-30 2008-08-29 Pulsed electrochemical sensor

Country Status (2)

Country Link
GB (1) GB0716877D0 (en)
WO (1) WO2009027708A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103675279B (en) * 2013-12-23 2015-04-08 山东理工大学 Preparation method for prostate tumor marker immunosensor
EP4350338A1 (en) * 2022-10-06 2024-04-10 Mettler-Toledo GmbH Sensor and device for a sensor comprising gel electrolyte

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DELAHAY P.: "Fundamentals of Coulostatic Analysis", ANAL. CHEM., vol. 34, no. 10, 1962, pages 1267 - 1271, XP002513897 *
DHARUMAN ET AL: "DNA hybridization detection on electrical microarrays using coulostatic pulse technique", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 22, no. 5, 15 December 2006 (2006-12-15), pages 744 - 751, XP005720844, ISSN: 0956-5663 *
KLEIN KARL-DITTMAR: "Potential relaxation as a measurement procedure for biosensors", GBF MONOGRAPHS; BIOSENSORS: FUNDAMENTALS, TECHNOLOGIES AND APPLICATIONS VCH VERLAGSGESELLSCHAFT MBH, POSTFACH 10 11 61, BOSCHSTRASSE 12, D-6940 WEINHEIM, GERMANY; VCH PUBLISHERS, INC., SUITE 909, 220 EAST 23RD STREET, NEW YORK, NEW YORK 10010, USA SE, 1992, & BMFT (BUNDESMINISTERIUM FUER FORSCHUNG UND TECHNOLOGIE); (NATIONAL MINISTRY OF RESEARCH AND TECHNOLO; BRANDENBURG, GERMANY; MAY 12-14, 1991, pages 333 - 336, XP009111531, ISSN: 3-527-28437-0 1-56081-220-6 *
KLEIN K-D: "Voltage relaxations of bi-electrodes for the long-term monitoring of concentrations of hydrogen peroxide, glucose, etc", SENSORS AND ACTUATORS B, ELSEVIER SEQUOIA S.A., LAUSANNE, CH, vol. 22, no. 2, 1 November 1994 (1994-11-01), pages 127 - 132, XP004012436, ISSN: 0925-4005 *

Also Published As

Publication number Publication date
WO2009027708A2 (en) 2009-03-05
GB0716877D0 (en) 2007-10-10

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