WO2008140620A3 - Ultra sensitive biomolecule detection using double stranded dna co-loaded gold nanoparticles and co-immobilized capture molecules - Google Patents

Ultra sensitive biomolecule detection using double stranded dna co-loaded gold nanoparticles and co-immobilized capture molecules Download PDF

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Publication number
WO2008140620A3
WO2008140620A3 PCT/US2007/088621 US2007088621W WO2008140620A3 WO 2008140620 A3 WO2008140620 A3 WO 2008140620A3 US 2007088621 W US2007088621 W US 2007088621W WO 2008140620 A3 WO2008140620 A3 WO 2008140620A3
Authority
WO
WIPO (PCT)
Prior art keywords
stranded dna
double stranded
gold nanoparticles
capture molecules
immobilized capture
Prior art date
Application number
PCT/US2007/088621
Other languages
French (fr)
Other versions
WO2008140620A2 (en
Inventor
Martin Huber
Original Assignee
Nanosphere Inc
Martin Huber
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere Inc, Martin Huber filed Critical Nanosphere Inc
Publication of WO2008140620A2 publication Critical patent/WO2008140620A2/en
Publication of WO2008140620A3 publication Critical patent/WO2008140620A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Abstract

A detection method, device is provided for detecting for the presence or absence of one or more target analytes such as proteins, nucleic acids, or other compounds derived from biological samples. The method, substrate and kit includes capture probes and capture oligonucleotide co-immobilized on discrete spots on the substrate to achieve ultra-sensitive level of detection for target analytes such as proteins, DNA, peptides, and small molecules.
PCT/US2007/088621 2006-12-21 2007-12-21 Ultra sensitive biomolecule detection using double stranded dna co-loaded gold nanoparticles and co-immobilized capture molecules WO2008140620A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87645306P 2006-12-21 2006-12-21
US60/876,453 2006-12-21

Publications (2)

Publication Number Publication Date
WO2008140620A2 WO2008140620A2 (en) 2008-11-20
WO2008140620A3 true WO2008140620A3 (en) 2009-01-08

Family

ID=39864692

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/088621 WO2008140620A2 (en) 2006-12-21 2007-12-21 Ultra sensitive biomolecule detection using double stranded dna co-loaded gold nanoparticles and co-immobilized capture molecules

Country Status (1)

Country Link
WO (1) WO2008140620A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111004836B (en) * 2019-12-24 2022-02-15 山东大学 Bidirectional amplification ratio type electrochemical aptamer sensor and application thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0921665D0 (en) * 2009-12-10 2010-01-27 Trillion Genomics Ltd Phobes
CN108410952A (en) * 2018-05-11 2018-08-17 重庆出入境检验检疫局检验检疫技术中心 The sandwich DNA hybridization of Listeria Monocytogenes, which quickly detects, uses probe, kit and detection method
WO2021007099A1 (en) * 2019-07-05 2021-01-14 Ultivue, Inc. Improved multiplexing method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030105320A1 (en) * 2001-08-31 2003-06-05 Becker Michael M. Affinity-shifted probes for quantifying analyte polynucleotides
WO2005003394A2 (en) * 2003-06-27 2005-01-13 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20050244954A1 (en) * 1998-06-23 2005-11-03 Blackburn Gary F Binding acceleration techniques for the detection of analytes
WO2006125050A2 (en) * 2005-05-17 2006-11-23 Nanosphere, Inc. Biobarcode assays for ultra high sensitive detection
US20060281076A1 (en) * 2005-05-18 2006-12-14 Nanosphere, Inc. Substrate functionalization method for high sensitivity applications

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244954A1 (en) * 1998-06-23 2005-11-03 Blackburn Gary F Binding acceleration techniques for the detection of analytes
US20030105320A1 (en) * 2001-08-31 2003-06-05 Becker Michael M. Affinity-shifted probes for quantifying analyte polynucleotides
WO2005003394A2 (en) * 2003-06-27 2005-01-13 Nanosphere, Inc. Bio-barcode based detection of target analytes
WO2006125050A2 (en) * 2005-05-17 2006-11-23 Nanosphere, Inc. Biobarcode assays for ultra high sensitive detection
US20060281076A1 (en) * 2005-05-18 2006-12-14 Nanosphere, Inc. Substrate functionalization method for high sensitivity applications

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
THAXTON ET AL: "Gold nanoparticle probes for the detection of nucleic acid targets", CLINICA CHIMICA ACTA, ELSEVIER BV, AMSTERDAM, NL, vol. 363, no. 1-2, 1 January 2006 (2006-01-01), pages 120 - 126, XP005194906, ISSN: 0009-8981 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111004836B (en) * 2019-12-24 2022-02-15 山东大学 Bidirectional amplification ratio type electrochemical aptamer sensor and application thereof

Also Published As

Publication number Publication date
WO2008140620A2 (en) 2008-11-20

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