WO2008135512A3 - Dna amplification method - Google Patents

Dna amplification method Download PDF

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Publication number
WO2008135512A3
WO2008135512A3 PCT/EP2008/055377 EP2008055377W WO2008135512A3 WO 2008135512 A3 WO2008135512 A3 WO 2008135512A3 EP 2008055377 W EP2008055377 W EP 2008055377W WO 2008135512 A3 WO2008135512 A3 WO 2008135512A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna methylation
amplification method
dna amplification
bisulfite
genome
Prior art date
Application number
PCT/EP2008/055377
Other languages
French (fr)
Other versions
WO2008135512A2 (en
Inventor
Jerzy Paszkowski
Jon Reinders
Original Assignee
Jerzy Paszkowski
Jon Reinders
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jerzy Paszkowski, Jon Reinders filed Critical Jerzy Paszkowski
Publication of WO2008135512A2 publication Critical patent/WO2008135512A2/en
Publication of WO2008135512A3 publication Critical patent/WO2008135512A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Abstract

The present invention is in the area of DNA methylation profiling. In particular, the invention relates to a method of using bisulfite-mediated cytosine conversion, followed by random amplification using a unique primer design, and processing for arrays or other high-throughput sequence analysis methods. The method according to the invention allows improved detection sensitivity and high resolution over the current state of the art of genome-wide detection of DNA methylation.
PCT/EP2008/055377 2007-05-02 2008-04-30 Dna amplification method WO2008135512A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008886 2007-05-02
EP07008886.9 2007-05-02

Publications (2)

Publication Number Publication Date
WO2008135512A2 WO2008135512A2 (en) 2008-11-13
WO2008135512A3 true WO2008135512A3 (en) 2009-01-08

Family

ID=39735183

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/055377 WO2008135512A2 (en) 2007-05-02 2008-04-30 Dna amplification method

Country Status (1)

Country Link
WO (1) WO2008135512A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005024053A1 (en) 2003-09-04 2005-03-17 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US7833942B2 (en) 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US8343738B2 (en) 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
WO2009067743A1 (en) * 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
US20130059734A1 (en) * 2009-11-13 2013-03-07 Commonwealth Scientific And Industrial Research Organisation Epigenetic analysis
MX350658B (en) 2011-09-07 2017-09-13 Human Genetic Signatures Pty Ltd Molecular detection assay.
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (en) 2013-02-08 2018-11-06 10X基因组学有限公司 Polynucleotides bar code generating at
KR20170012552A (en) * 2014-06-05 2017-02-02 클리니칼 게노믹스 피티와이. 엘티디. Method for methylation analysis
EP3889325A1 (en) 2014-06-26 2021-10-06 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CN113481281A (en) * 2014-10-08 2021-10-08 康奈尔大学 Method for identifying one or more nucleic acid molecules containing a target nucleotide sequence in a sample
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3497220A4 (en) 2016-08-10 2020-04-01 Grail, Inc. Methods of preparing dual-indexed dna libraries for bisulfite conversion sequencing
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN109526228B (en) 2017-05-26 2022-11-25 10X基因组学有限公司 Single cell analysis of transposase accessible chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN108085372B (en) * 2018-01-02 2021-09-28 江洪 Primer end enzymolysis dimer method PCR

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US6977146B1 (en) * 1999-01-29 2005-12-20 Epigenomics Ag Method of identifying cytosine methylation patterns in genomic DNA samples
WO2006113770A1 (en) * 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US6977146B1 (en) * 1999-01-29 2005-12-20 Epigenomics Ag Method of identifying cytosine methylation patterns in genomic DNA samples
WO2006113770A1 (en) * 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AFFYMETRIX: "GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual", 2006, XP002497868 *
ZHANG XIAOYU ET AL: "Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis", September 2006, CELL, VOL. 126, NR. 6, PAGE(S) 1189-1201, ISSN: 0092-8674, XP002497869 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing

Also Published As

Publication number Publication date
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