WO2008132524A1 - Novel apiary composition - Google Patents

Novel apiary composition Download PDF

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Publication number
WO2008132524A1
WO2008132524A1 PCT/HU2008/000038 HU2008000038W WO2008132524A1 WO 2008132524 A1 WO2008132524 A1 WO 2008132524A1 HU 2008000038 W HU2008000038 W HU 2008000038W WO 2008132524 A1 WO2008132524 A1 WO 2008132524A1
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WIPO (PCT)
Prior art keywords
oil
organum
marjoram
weight
thymol
Prior art date
Application number
PCT/HU2008/000038
Other languages
French (fr)
Inventor
Gyula Orbán
Original Assignee
Chemor Kutató, Fejlesztõ És Kereskedõ Kft.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Chemor Kutató, Fejlesztõ És Kereskedõ Kft. filed Critical Chemor Kutató, Fejlesztõ És Kereskedõ Kft.
Priority to EP08750828A priority Critical patent/EP2148668A1/en
Publication of WO2008132524A1 publication Critical patent/WO2008132524A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K51/00—Appliances for treating beehives or parts thereof, e.g. for cleaning or disinfecting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/05—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/52—Juglandaceae (Walnut family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

Definitions

  • the invention relates to a novel apiary composition which is useful for preventing American Foulbrood disease (Histolysis infectiosa perniciosa larvarum) of honey bees (Apis mellifera) and for treatment of the established disease.
  • American Foulbrood disease Histolysis infectiosa perniciosa larvarum
  • honey bees Apis mellifera
  • Foulbrood disease is an ancient disease of the brood of honey bees which had already been reported in ancient times.
  • Pathogen of the disease is the bacterium Paenibacillus larvae.
  • the vegetative form of the microorganism is bacilliform with cilia on all sides, serving for motion. Inside of the bacilli, spore formations are present. While vegetative bacilli are not resistant, the spores retain their germinative ability for more than 10 years. It is characteristic to the resistance of the spores the surviving ability in honey boiled for 10 minutes while for total destruction at least heating at 112 0 C for 1 hour is necessary.
  • Foulbrood disease may occur any time in the brooding period.
  • the natural progress of extension of the infection caused by the pathogen is slow, espe- cially as the adult bees remove most of the spores and only the youngest larva is specifically susceptible to the infection.
  • Adult bees never get sick.
  • the color of the decayed larva turns into brown from china white and long, sticky thread can be stretched from the body. Its smell reminds of glue.
  • the dried rest of body jams against the cell wall or bottom and turns to a scutum-like crust.
  • the crust comprises millions of spores of the pathogen thus forming a further source of infection. Destruction of the brooding gets stronger and stronger and extension of the disease may even result in a complete destruction of the family.
  • the honey supply of the destructed colony will be car- ried off by other strong, healthy colonies and thus, the disease could be expanded.
  • the disease can also spread by hiving swarms of bees of unknown origin or through instruments originating from other infected apicultures.
  • foulbrood disease may be combated by two ba- sic methods: one is the radical route, the other may be some kind of medication.
  • the radical route In case of the radical route, the diseased bee colonies are killed and the instruments capable of spreading the infection are destroyed (burned). Wherein the extension of disease is of lower grade, usually the radical route is applied (for example, in Hungary). Medical treatment is usually used only in countries where the disease has already strongly extended and there is no hope for localization or possibly for full elimination.
  • the ethylene dioxide method has become known from the 1960's, which consists of disinfecting the honeycomb for 18 hours at 27 0 C. Though this method is effective, however expensive, complicated and dangerous (the ethylene dioxide is explosive). Thus, this method has not been widely extended in the profession and remained in the experimental phase only. It is apparent therefore, that no method or medication has been known which would have successfully prevented the disease or cured the disease already developed.
  • the first step of the experiments was culturing the pathogen.
  • the long-term experiments were carried out by agar diffusion methods.
  • the method consisted of dripping the test material of defined quantity, concurrently with the application of the pathogen, in the holes of solid medium in Petri plates.
  • the plates were incubated at permanent temperatures and thus, after 72 hours, well-defined and measurable inhibition zones were obtained.
  • the sizes of the inhibition zones were used for evaluating the activity of the test materials used, i.e. a higher inhibition zone was evaluated as more effective (measured in mm, by deducting the diameter of the hole).
  • the experiments aimed to select the best composition meeting the above criteria.
  • A the active materials were applied in an amount of 30 ⁇ l (1 drop)
  • B the active materials were applied in an amount of 60 ⁇ l (2 drop)
  • C the concentration of the test materials (in case of columns 2, 3, 4, 5 and 8) was raised from 0,1 % to 0,5% and applied in an amount of 30 ⁇ l (1 drop)
  • K the size of inhibition zone is not detectable as the pathogen colonies on the entire area were blast.
  • the thymol solution itself is less active than the thymol dissolved in the herbal extract
  • any one of the hyssop oil combinations is less active than those of the other two oils (5 ⁇ 4 ⁇ 3 ⁇ 2),
  • composition No. 2 possesses extraordinary results
  • the invention relates to an apiary composition
  • an apiary composition comprising as active ingredients
  • volatile oil and/or a component of a volatile oil selected from the group consisting of cinnamon oil, anisic oil, hyssop oil, thymol,
  • an aqueous-alcoholic extract of herbs selected from the group consisting thyme (Thymus vulgaris), wild marjoram (Organum vulgare), walnut leaf (Junglandis folium), marjoram (Organum majoranna), with carriers and auxiliaries known per se, preferably water and ethanol.
  • the composition contains 1-5% by weight thymol, 0,1-3 % by weight cinnamon oil, 0,1-1 % by weight anisic oil and an aqueous-alcoholic extract of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare) along with carriers and auxiliaries known perse, preferably water and ethanol.
  • the composition contains as active ingredient 1-4% by weight thymol, 0,1-3 % by weight cinnamon oil, 0,1-1 % by weight anisic oil and an aqueous-alcoholic extract of thyme (Thymus vulgaris), walnut leaf (Junglandis folium), marjoram (Organum majoranna) and wild marjoram (Or- ganum vulgare) along with carriers and auxiliaries known per se, preferably water and ethanol.
  • the herbal extract component of the composition according to the invention is prepared by well-known extracting methods (the process is illustrated in the following examples).
  • anisic oil (Aetheroleum anisi) dissolved in the aqueous-alcoholic extract of the herb combination consisting of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare). 2. 4% by weight thymol (3-p-cimenol)
  • cinnamon oil (Aetheroleum cinnamoni) dissolved in the aqueous-alcoholic extract of the herb combination consisting of thyme (Thymus vulgaris) and wild marjoram (Organum vulgare).
  • cinnamon oil (Aetheroleum cinnamoni) dissolved in the aqueous-alcoholic extract of the herb combination consisting of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Or- ganum majoranna) and wild marjoram (Organum vulgare).
  • cinnamon oil (Aetheroleum cinnamoni) dissolved in the aqueous-alcoholic extract of the herb combination consisting of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare).

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to an apiary composition comprising as active ingredients (i) volatile oil and/or a component of a volatile oil selected from the group consisting of cinnamon oil, anisic oil, hyssop oil, thymol, (ii) an aqueous-alcoholic extract of herbs selected from the group consisting thyme (Thymus vulgaris), wild marjoram (Organum vulgare), walnut leaf (Junglandis folium), marjoram (Organum majoranna), with carriers and auxiliaries known per se, preferably water and ethanol.

Description

Novel apiary composition
Technical field
The invention relates to a novel apiary composition which is useful for preventing American Foulbrood disease (Histolysis infectiosa perniciosa larvarum) of honey bees (Apis mellifera) and for treatment of the established disease.
Background
Foulbrood disease is an ancient disease of the brood of honey bees which had already been reported in ancient times. Pathogen of the disease is the bacterium Paenibacillus larvae. The vegetative form of the microorganism is bacilliform with cilia on all sides, serving for motion. Inside of the bacilli, spore formations are present. While vegetative bacilli are not resistant, the spores retain their germinative ability for more than 10 years. It is characteristic to the resistance of the spores the surviving ability in honey boiled for 10 minutes while for total destruction at least heating at 1120C for 1 hour is necessary.
Foulbrood disease may occur any time in the brooding period. The natural progress of extension of the infection caused by the pathogen is slow, espe- cially as the adult bees remove most of the spores and only the youngest larva is specifically susceptible to the infection. Adult bees never get sick.
However, the infected larva dies from the toxin produced by the P. larvae. All this takes place after termination of brooding, after the larva ceases feeding.
The color of the decayed larva turns into brown from china white and long, sticky thread can be stretched from the body. Its smell reminds of glue. Finally, the dried rest of body, jams against the cell wall or bottom and turns to a scutum-like crust. The crust comprises millions of spores of the pathogen thus forming a further source of infection. Destruction of the brooding gets stronger and stronger and extension of the disease may even result in a complete destruction of the family. The honey supply of the destructed colony will be car- ried off by other strong, healthy colonies and thus, the disease could be expanded. The disease can also spread by hiving swarms of bees of unknown origin or through instruments originating from other infected apicultures.
It is well known in the art that foulbrood disease may be combated by two ba- sic methods: one is the radical route, the other may be some kind of medication.
In case of the radical route, the diseased bee colonies are killed and the instruments capable of spreading the infection are destroyed (burned). Wherein the extension of disease is of lower grade, usually the radical route is applied (for example, in Hungary). Medical treatment is usually used only in countries where the disease has already strongly extended and there is no hope for localization or possibly for full elimination.
Until the appearance of antibiotics, sulfonamides were used as medical treatment, with little success. Since the 1950's, oxytetracycline-type antibiotics have been used, but even such treatment have not brought sufficient success. Antibiotics (but even sulfonamides) destroy the vegetative form of the pathogen but not the spores. After termination of medication, the disease may be resumed any time. An other backlog of the treatment is the contamination of the honey which may be hazardous to the consumer.
The ethylene dioxide method has become known from the 1960's, which consists of disinfecting the honeycomb for 18 hours at 270C. Though this method is effective, however expensive, complicated and dangerous (the ethylene dioxide is explosive). Thus, this method has not been widely extended in the profession and remained in the experimental phase only. It is apparent therefore, that no method or medication has been known which would have successfully prevented the disease or cured the disease already developed.
Disclosure of Invention
It is the aim of the present invention to provide an effective composition which, by a simple administration method, successfully combats the disease or prevents foulbrood disease.
Our experiments were focused on the development of natural (herbal) -based compositions. The aim of the long in vitro experiments has been to develop compositions of natural base which effectively and significantly inhibit or kill the pathogen (Paenibacillus larvae ) bred artificially.
The first step of the experiments was culturing the pathogen. The long-term experiments were carried out by agar diffusion methods. The method consisted of dripping the test material of defined quantity, concurrently with the application of the pathogen, in the holes of solid medium in Petri plates. The plates were incubated at permanent temperatures and thus, after 72 hours, well-defined and measurable inhibition zones were obtained. The sizes of the inhibition zones were used for evaluating the activity of the test materials used, i.e. a higher inhibition zone was evaluated as more effective (measured in mm, by deducting the diameter of the hole). The experiments aimed to select the best composition meeting the above criteria.
The experiments have shown that some combinations of the active substances of natural origin, i.e. herbal origin, possessed extremely good effect. These good results could significantly be improved by complementing the combinations by the addition of thymol (which is a component of many volatile oils) and/or some volatile oils, especially cinnamon and anise oil. The experimental results are shown in the following table:
Figure imgf000005_0001
Explanation:
The meanings of the capital letters in the table are as follows:
A: the active materials were applied in an amount of 30μl (1 drop) B: the active materials were applied in an amount of 60μl (2 drop) C: the concentration of the test materials (in case of columns 2, 3, 4, 5 and 8) was raised from 0,1 % to 0,5% and applied in an amount of 30μl (1 drop) K: the size of inhibition zone is not detectable as the pathogen colonies on the entire area were blast.
The bold numbers of the first column have the following meaning:
1. herbs (4 different) in aqueous alcoholic extract + 2,0% thymol
2. herbs (4 different) in aqueous alcoholic extract + 2,2% thymol + 0,1% cinnamon oil 3. as item 2 + 0, 1 % anisic oil 4. as item 3 + 0,1 % hyssop oil 5. as item 2 + 0,1% hyssop oil
6. 20% thymol dissolved in 20% alcohol in water
7. 1 % aqueous sorbic acid solution
8. as item 1 + 0,8% cinnamon oil + 0,2% anisic oil
The conclusions drawn from the above table are as follows:
- the sorbic acid itself is inactive,
- the thymol solution itself is less active than the thymol dissolved in the herbal extract,
- any one of the hyssop oil combinations is less active than those of the other two oils (5<4<3<2),
- composition No. 2 possesses extraordinary results,
- combination No. 8 shows very good results as well.
Accordingly, the invention relates to an apiary composition comprising as active ingredients
(i) volatile oil and/or a component of a volatile oil selected from the group consisting of cinnamon oil, anisic oil, hyssop oil, thymol,
(ii) an aqueous-alcoholic extract of herbs selected from the group consisting thyme (Thymus vulgaris), wild marjoram (Organum vulgare), walnut leaf (Junglandis folium), marjoram (Organum majoranna), with carriers and auxiliaries known per se, preferably water and ethanol.
In a preferred embodiment of the invention the composition contains 1-5% by weight thymol, 0,1-3 % by weight cinnamon oil, 0,1-1 % by weight anisic oil and an aqueous-alcoholic extract of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare) along with carriers and auxiliaries known perse, preferably water and ethanol.
According to an other preferred embodiment of the invention the composition contains as active ingredient 1-4% by weight thymol, 0,1-3 % by weight cinnamon oil, 0,1-1 % by weight anisic oil and an aqueous-alcoholic extract of thyme (Thymus vulgaris), walnut leaf (Junglandis folium), marjoram (Organum majoranna) and wild marjoram (Or- ganum vulgare) along with carriers and auxiliaries known per se, preferably water and ethanol.
The herbal extract component of the composition according to the invention is prepared by well-known extracting methods (the process is illustrated in the following examples).
Best mode of Carrying out the Invention
Preliminary in vivo tests carried out with the compositions according to the invention fully confirmed the favorable in vitro results. The in vivo tests were carried our with the compositions as illustrated below:
1. 2% by weight thymol (3-p-cimenol) 0,8% by weight cinnamon oil (Aetheroleum cinnamoni)
0,2% by weight anisic oil (Aetheroleum anisi) dissolved in the aqueous-alcoholic extract of the herb combination consisting of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare). 2. 4% by weight thymol (3-p-cimenol)
1 ,0% by weight cinnamon oil (Aetheroleum cinnamoni) dissolved in the aqueous-alcoholic extract of the herb combination consisting of thyme (Thymus vulgaris) and wild marjoram (Organum vulgare).
3. 2% by weight thymol (3-p-cimenol)
0,8% by weight cinnamon oil (Aetheroleum cinnamoni) dissolved in the aqueous-alcoholic extract of the herb combination consisting of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Or- ganum majoranna) and wild marjoram (Organum vulgare).
4. 2% by weight thymol (3-p-cimenol)
1 ,0% by weight cinnamon oil (Aetheroleum cinnamoni) dissolved in the aqueous-alcoholic extract of the herb combination consisting of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare).
The following examples serve for illustrating the invention more in details. It is, however, to be understood that the scope of the invention may no way be Nm- ited to those described in the examples.
Example 1
Preparation of 100 ml herb extract:
Into 70 ml water at 7O0C 4 g walnut leaf, 8 g thyme, 4 g marjoram and 8 g wild marjoram are placed and stirred for 2 hours. The mixture is completed with 45 ml water of 2O0C and 25 ml 96% ethyl alcohol. The slurry is kept at a temperature of 38-420C and stirred for 5 minutes in 30 minutes intervals.
After 48 hours extraction, the plant phase is separated from the fluid. The alcohol content of the extract is adjusted to 15-20% (v/v). Example 2
Apiary composition
93 ml herbal extract of Example 1 4 ml ethyl alcohol 2 g thymol 1 ml cinnamic oil
Example 3
Apiary composition
93 ml herbal extract of Example 1
4 ml ethyl alcohol
2 g thymol
0,8 ml cinnamic oil 0,2 ml anisic oil

Claims

Claims
1. An apiary composition comprising as active ingredients
(i) volatile oil and/or a component of a volatile oil selected from the group consisting of cinnamon oil, anisic oil, hyssop oil, thymol,
(ii) an aqueous-alcoholic extract of herbs selected from the group consisting thyme (Thymus vulgaris), wild marjoram (Organum vulgare), walnut leaf (Junglandis folium), marjoram (Organum majoranna), with carriers and auxiliaries known per se, preferably water and ethanol.
2. The composition according to claim 1 which contains
1-5% by weight thymol, 0,1-3 % by weight cinnamon oil, optionally 0,1-1 % by weight anisic oil and an aqueous-alcoholic extract of thyme (Thymus vulgaris), wild marjoram (Organum vulgare), optionally walnut leaf (Junglandis folium) and marjoram (Organum majoranna) along with carriers and auxiliaries known perse, preferably water and ethanol.
3. The composition according to claim 1 which contains
1-4% by weight thymol, 0,1-2 % by weight cinnamon oil, 0,1-1 % by weight anisic oil and an aqueous-alcoholic extract of walnut leaf (Junglandis folium), thyme (Thymus vulgaris), marjoram (Organum majoranna) and wild marjoram (Organum vulgare) along with carriers and auxiliaries known perse, preferably water and ethanol.
PCT/HU2008/000038 2007-04-27 2008-04-24 Novel apiary composition WO2008132524A1 (en)

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HUP0700310 2007-04-27
HU0700310A HUP0700310A2 (en) 2007-04-27 2007-04-27 New veterinary product for honeybees

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102273495A (en) * 2010-06-11 2011-12-14 滕纯森 Baofengjian
ITRM20110450A1 (en) * 2011-08-26 2013-02-27 Vecchio Francesca Del EQUIPMENT METHOD INGREDIENTS AND FORMULATIONS FOR PRODUCING SOLUTIONS WITH A HIGH NOURISHING AND CURIOUS RECONSTITUTING NOURISHING EFFECT FOR A COMPLETE AND AUTOMATED SYSTEM TO PREVENT AND FIGHT THE BEALS SPLASH SYNDROME
RU2496310C1 (en) * 2012-06-22 2013-10-27 Общество с ограниченной ответственностью "УНИФАРМ" (ООО "УНИФАРМ") Agent for prevention and treatment of bees and bee brood
WO2015113175A1 (en) 2014-01-30 2015-08-06 Universidad Del Desarrollo Biocide composition for controlling pests affecting european honey bees, comprising a water-soluble olea europaea extract
WO2017091915A1 (en) 2015-12-02 2017-06-08 Universidad De Chile Composition, method and use for controlling fungal infection caused by nosema ceranae fungus in apis mellifera bee colonies, comprising application, as a syrup (pj-cd14) and aerosol (pa-cd14), of an effective quantity of an essential oil (cd14) obtained from cryptocaria alba (peumo) leaves

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Title
ALIPPI A M ET AL: "Antimicrobial activity of some essential oils against Paenibacillus larvae, the causal agent of American Foulbrood Disease", JOURNAL OF HERBS, SPICES AND MEDICINAL PLANTS, FOOD PRODUCTS PRESS, BINGHAM, NY, US, vol. 4, no. 2, 1 January 1996 (1996-01-01), pages 9 - 16, XP009103416, ISSN: 1049-6475 *
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102273495A (en) * 2010-06-11 2011-12-14 滕纯森 Baofengjian
ITRM20110450A1 (en) * 2011-08-26 2013-02-27 Vecchio Francesca Del EQUIPMENT METHOD INGREDIENTS AND FORMULATIONS FOR PRODUCING SOLUTIONS WITH A HIGH NOURISHING AND CURIOUS RECONSTITUTING NOURISHING EFFECT FOR A COMPLETE AND AUTOMATED SYSTEM TO PREVENT AND FIGHT THE BEALS SPLASH SYNDROME
WO2013030854A1 (en) * 2011-08-26 2013-03-07 Beesfree Inc. Method, apparatus and compositions for the prophylaxis and treatment of colony collapse disorder
US9981001B2 (en) 2011-08-26 2018-05-29 Healthy Bees, Llc. Method, apparatus and compositions for the prophylaxis and treatment of colony collapse disorder
RU2496310C1 (en) * 2012-06-22 2013-10-27 Общество с ограниченной ответственностью "УНИФАРМ" (ООО "УНИФАРМ") Agent for prevention and treatment of bees and bee brood
WO2015113175A1 (en) 2014-01-30 2015-08-06 Universidad Del Desarrollo Biocide composition for controlling pests affecting european honey bees, comprising a water-soluble olea europaea extract
WO2017091915A1 (en) 2015-12-02 2017-06-08 Universidad De Chile Composition, method and use for controlling fungal infection caused by nosema ceranae fungus in apis mellifera bee colonies, comprising application, as a syrup (pj-cd14) and aerosol (pa-cd14), of an effective quantity of an essential oil (cd14) obtained from cryptocaria alba (peumo) leaves

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