WO2008123019A1 - Method of detecting change in gene and detection device - Google Patents

Method of detecting change in gene and detection device Download PDF

Info

Publication number
WO2008123019A1
WO2008123019A1 PCT/JP2008/050895 JP2008050895W WO2008123019A1 WO 2008123019 A1 WO2008123019 A1 WO 2008123019A1 JP 2008050895 W JP2008050895 W JP 2008050895W WO 2008123019 A1 WO2008123019 A1 WO 2008123019A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
gene
acid amplification
reaction container
change
Prior art date
Application number
PCT/JP2008/050895
Other languages
French (fr)
Japanese (ja)
Inventor
Hiromi Sanuki
Shigeru Komiyama
Original Assignee
Olympus Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corporation filed Critical Olympus Corporation
Priority to JP2008533810A priority Critical patent/JPWO2008123019A1/en
Publication of WO2008123019A1 publication Critical patent/WO2008123019A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

It is intended to provide a technique suitable for the automated detection whereby a change in a gene in a minute amount of a biological sample can be efficiently detected. Namely, a method of detecting a change in a gene which comprises: the step of extracting a nucleic acid from a biological sample; the step of pipetting 0.5 to 5 μl portions of the nucleic acid solution obtained in the above step 1) into different positions on a single reaction container located on a reaction container table allowing temperature control; the step of drying the nucleic acid solution on the reaction container by controlling the temperature of the unit allowing temperature control; the step of pipetting 0.5 to 5 μl portions of a nucleic acid amplification reagent to the dried nucleic acid on the reaction container; the step of pipetting 3 to 10 μl portions of a solution for preventing the nucleic acid amplification solution from evaporation to the nucleic acid amplification reagent pipetted on the above step; and the step of conducting the nucleic acid amplification reaction by controlling the temperature of the unit allowing temperature control; followed by the detection of the product obtained after the completion of the nucleic acid amplification reaction and the analysis of the change in the gene.
PCT/JP2008/050895 2007-03-05 2008-01-23 Method of detecting change in gene and detection device WO2008123019A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008533810A JPWO2008123019A1 (en) 2007-03-05 2008-01-23 Gene change detection method and detection apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-054708 2007-03-05
JP2007054708 2007-03-05

Publications (1)

Publication Number Publication Date
WO2008123019A1 true WO2008123019A1 (en) 2008-10-16

Family

ID=39830522

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050895 WO2008123019A1 (en) 2007-03-05 2008-01-23 Method of detecting change in gene and detection device

Country Status (2)

Country Link
JP (1) JPWO2008123019A1 (en)
WO (1) WO2008123019A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011024947A1 (en) * 2009-08-31 2011-03-03 株式会社ハウステック Molded structure
WO2011040504A1 (en) * 2009-09-30 2011-04-07 凸版印刷株式会社 Nucleic acid analyzer
JP2012075413A (en) * 2010-10-05 2012-04-19 Hitachi High-Technologies Corp Apparatus for inspecting nucleic acid and method of conveying container

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001149097A (en) * 1999-11-29 2001-06-05 Olympus Optical Co Ltd Automatic nucleic acid examination apparatus
WO2002025289A1 (en) * 2000-09-18 2002-03-28 I-Card Corporation Micro well array and method of sealing liquid using the micro well array
JP2003061684A (en) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Method for dna base sequence determination
JP2003304899A (en) * 2002-04-11 2003-10-28 Yoshinobu Baba Method and apparatus for analyzing nucleic acid
JP2005515792A (en) * 2002-01-29 2005-06-02 グローバル ジェノミクス アクティエボラーグ Methods and means for manipulating nucleic acids

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001149097A (en) * 1999-11-29 2001-06-05 Olympus Optical Co Ltd Automatic nucleic acid examination apparatus
WO2002025289A1 (en) * 2000-09-18 2002-03-28 I-Card Corporation Micro well array and method of sealing liquid using the micro well array
JP2003061684A (en) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Method for dna base sequence determination
JP2005515792A (en) * 2002-01-29 2005-06-02 グローバル ジェノミクス アクティエボラーグ Methods and means for manipulating nucleic acids
JP2003304899A (en) * 2002-04-11 2003-10-28 Yoshinobu Baba Method and apparatus for analyzing nucleic acid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TOJO H. ET AL.: "A rapid methods for screening transgenic mice by PCR with small amounts of blood", EXP. ANIM., vol. 40, no. 2, 1991, pages 235 - 238 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011024947A1 (en) * 2009-08-31 2011-03-03 株式会社ハウステック Molded structure
JP2011053334A (en) * 2009-08-31 2011-03-17 Housetec Inc Molded structure
WO2011040504A1 (en) * 2009-09-30 2011-04-07 凸版印刷株式会社 Nucleic acid analyzer
JP2012161340A (en) * 2009-09-30 2012-08-30 Toppan Printing Co Ltd Nucleic acid analyzer, and method for isolating and purifying nucleic acid
US9267890B2 (en) 2009-09-30 2016-02-23 Toppan Printing Co., Ltd. Nucleic acid analyzer
JP2012075413A (en) * 2010-10-05 2012-04-19 Hitachi High-Technologies Corp Apparatus for inspecting nucleic acid and method of conveying container

Also Published As

Publication number Publication date
JPWO2008123019A1 (en) 2010-07-15

Similar Documents

Publication Publication Date Title
WO2007044974A3 (en) Absolute pcr quantification
WO2007002567A3 (en) Selective isolation and concentration of nucleic acids from complex samples
WO2007044091A3 (en) Analysis using microfluidic partitioning devices
WO2006117161A3 (en) New labelling strategies for the sensitive detection of analytes
EP1661988A4 (en) Kit for nucleic acid detection
WO2010104345A2 (en) Apparatus for integrated real-time nucleic acid analysis, and method for detecting a target nucleic acid using same
WO2012049066A3 (en) Real-time amplification and micro-array based detection of nucleic acid targets in a flow chip assay
WO2004106547A3 (en) Fast method for detecting micro-organisms in food samples
WO2006053770A8 (en) Real-time pcr of targets on a micro-array
WO2007024840A3 (en) Method of quantitating nucleic acids by flow cytometry microparticle-based array
WO2012057548A3 (en) Automatic real-time pcr system for the various analysis of biological smaple
EP2484781A3 (en) Detection of analytes and nucleic acids
EP1375673A4 (en) Gene analysis method and analyzer therefor
WO2006077102A3 (en) Method for the detection of hpv and probes, primers and kits
EP1795612A4 (en) Nucleic acid analysis method
WO2008136868A3 (en) Detection and quantification of biomolecules using mass spectrometry
WO2008034896A3 (en) Identification and quantification of a plurality of biological (micro)organisms or their components
WO2009007719A3 (en) Detection of micro-organisms based on their nad-dependent dna ligase activity
EP1890146A4 (en) Method for detecting of nucleic acid
WO2007087336A3 (en) Methods for detecting nucleic acids using multiple signals
EP1829964A4 (en) Method of examining gene sequence
WO2008123019A1 (en) Method of detecting change in gene and detection device
EP1721991A4 (en) Method of detecting nucleic acid and utilization thereof
WO2008054432A3 (en) Oligonucleotide microarray for identification of pathogens
WO2007060707A3 (en) Reagents and method for simultaneous nucleic acid amplification and detection

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 2008533810

Country of ref document: JP

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08703727

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08703727

Country of ref document: EP

Kind code of ref document: A1