WO2008119009A3 - Materials and methods for efficient alanine production - Google Patents

Materials and methods for efficient alanine production Download PDF

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Publication number
WO2008119009A3
WO2008119009A3 PCT/US2008/058410 US2008058410W WO2008119009A3 WO 2008119009 A3 WO2008119009 A3 WO 2008119009A3 US 2008058410 W US2008058410 W US 2008058410W WO 2008119009 A3 WO2008119009 A3 WO 2008119009A3
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WO
WIPO (PCT)
Prior art keywords
sugars
alanine
efficient
materials
methods
Prior art date
Application number
PCT/US2008/058410
Other languages
French (fr)
Other versions
WO2008119009A2 (en
WO2008119009A4 (en
Inventor
Xueli Zhang Zhang
Kaemwich Jantama
Jonathan C Moore
Keelnatham T Shanmugam
Lonnie O'neal Ingram
Original Assignee
Univ Florida
Xueli Zhang Zhang
Kaemwich Jantama
Jonathan C Moore
Keelnatham T Shanmugam
Lonnie O'neal Ingram
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida, Xueli Zhang Zhang, Kaemwich Jantama, Jonathan C Moore, Keelnatham T Shanmugam, Lonnie O'neal Ingram filed Critical Univ Florida
Publication of WO2008119009A2 publication Critical patent/WO2008119009A2/en
Publication of WO2008119009A3 publication Critical patent/WO2008119009A3/en
Publication of WO2008119009A4 publication Critical patent/WO2008119009A4/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The subject application provides genetically engineered microorganisms that produce L-alanine as the primary fermentation product from sugars. Pentose sugars, such as xylose, and hexose sugars, such as glucose, can be effectively fermented to L-alanine. The strains described herein have the ability to metabolize all sugars that are constituents of lignocellulosic biomass and a variety of disaccharides, including lactose, maltose, sucrose and others.
PCT/US2008/058410 2007-03-27 2008-03-27 Materials and methods for efficient alanine production WO2008119009A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90823407P 2007-03-27 2007-03-27
US60/908,234 2007-03-27

Publications (3)

Publication Number Publication Date
WO2008119009A2 WO2008119009A2 (en) 2008-10-02
WO2008119009A3 true WO2008119009A3 (en) 2009-02-26
WO2008119009A4 WO2008119009A4 (en) 2009-04-16

Family

ID=39789270

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/058410 WO2008119009A2 (en) 2007-03-27 2008-03-27 Materials and methods for efficient alanine production

Country Status (1)

Country Link
WO (1) WO2008119009A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040152159A1 (en) 2002-11-06 2004-08-05 Causey Thomas B. Materials and methods for the efficient production of acetate and other products
AU2008228948B2 (en) 2007-03-20 2014-04-24 University Of Florida Research Foundation, Inc. Materials and methods for efficient succinate and malate production
WO2010059616A2 (en) 2008-11-18 2010-05-27 University Of Florida Research Foundation, Inc. Biocatalysts and methods for conversion of hemicellulose hydrolsates to biobased products
AU2010232533A1 (en) 2009-04-02 2011-10-13 University Of Florida Research Foundation, Inc. Engineering the pathway for succinate production
CN101974476A (en) * 2010-08-31 2011-02-16 安徽华恒生物工程有限公司 XZ-A26 bacterial strain for producing L-alanine with high yield as well as construction method and application thereof
US20150247174A1 (en) * 2012-12-28 2015-09-03 Anhui Huaheng Bioengineering Co., Ltd, Engineering bacteria for producing dl-alanine and method for producing dl-alanine by using engineering bacteria
CN103045528B (en) * 2012-12-28 2014-05-07 安徽华恒生物工程有限公司 Engineering bacteria producing DL-alanine and method of producing DL-alanine by using engineering bacteria
JP2016529901A (en) * 2013-08-30 2016-09-29 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ Modified microorganisms for improving alanine production
JP6685910B2 (en) * 2013-12-13 2020-04-22 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ Recombinant microorganisms for improving fine chemical production
JP2017514464A (en) * 2014-04-30 2017-06-08 エボニック デグサ ゲーエムベーハーＥｖｏｎｉｋ Ｄｅｇｕｓｓａ ＧｍｂＨ Method for producing L-amino acid using alkalophilic bacteria
JP6914846B2 (en) 2015-03-18 2021-08-04 ビーエイエスエフ・ソシエタス・エウロパエアＢａｓｆ Ｓｅ Recombinant microorganisms for improving fine chemical production
CN104774790B (en) * 2015-04-03 2020-11-06 江南大学 Escherichia coli for efficiently fermenting and producing L-alanine
WO2016193351A2 (en) 2015-06-04 2016-12-08 Basf Se Recombinant microorganism for improved production of fine chemicals
MY189932A (en) * 2015-06-12 2022-03-22 Basf Se Recombinant microorganism for improved production of alanine
CN110904062B (en) * 2018-09-18 2022-03-15 安徽华恒生物科技股份有限公司 Strain capable of producing L-alanine at high yield
CN110982857A (en) * 2019-09-23 2020-04-10 安徽丰原生物化学股份有限公司 Fermentation production method of L-alanine
CN114457123B (en) * 2020-05-13 2023-06-20 安徽华恒生物科技股份有限公司 Recombinant microorganism for producing L-valine as well as construction method and application thereof
CN115960942A (en) * 2021-10-08 2023-04-14 安徽丰原生物技术股份有限公司 Genetic engineering strain for producing L-alanine and construction method and application thereof
CN115960941A (en) * 2021-10-08 2023-04-14 安徽丰原生物技术股份有限公司 Genetic engineering strain for producing DL-alanine and construction method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6372457B1 (en) * 1997-01-14 2002-04-16 Arkion Life Sciences Llc Process and materials for production of glucosamine
US7037504B2 (en) * 2001-10-23 2006-05-02 Waratah Pharmaceuticals, Inc. Epidermal growth factor protein and gene, and methods of use therefor
US7052883B2 (en) * 2001-04-03 2006-05-30 Degussa Ag Process for the production of L-amino acids using strains of the family Enterobacteriaceae that contain an attenuated fruR gene
US7083942B2 (en) * 2002-03-09 2006-08-01 Degussa Ag Alleles of the aceA gene from coryneform bacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6372457B1 (en) * 1997-01-14 2002-04-16 Arkion Life Sciences Llc Process and materials for production of glucosamine
US7052883B2 (en) * 2001-04-03 2006-05-30 Degussa Ag Process for the production of L-amino acids using strains of the family Enterobacteriaceae that contain an attenuated fruR gene
US7037504B2 (en) * 2001-10-23 2006-05-02 Waratah Pharmaceuticals, Inc. Epidermal growth factor protein and gene, and methods of use therefor
US7083942B2 (en) * 2002-03-09 2006-08-01 Degussa Ag Alleles of the aceA gene from coryneform bacteria

Also Published As

Publication number Publication date
WO2008119009A2 (en) 2008-10-02
WO2008119009A4 (en) 2009-04-16

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