WO2008099451A1 - Method of designing high-functional protein by comprehensive amino acid substitution - Google Patents
Method of designing high-functional protein by comprehensive amino acid substitution Download PDFInfo
- Publication number
- WO2008099451A1 WO2008099451A1 PCT/JP2007/052342 JP2007052342W WO2008099451A1 WO 2008099451 A1 WO2008099451 A1 WO 2008099451A1 JP 2007052342 W JP2007052342 W JP 2007052342W WO 2008099451 A1 WO2008099451 A1 WO 2008099451A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- prion
- prion protein
- amino acid
- type
- acid substitution
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2790/00—Viroids or subviral agents
- C12N2790/00011—Details
- C12N2790/10011—Prions
- C12N2790/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
It is intended to design a prion protein molecule, which is easily converted into an abnormal prion, by transferring a point mutation into a prion protein. Namely, a method of designing a prion protein having an amino acid substitution, whereby the conversion ability of a prion protein from a normal type into an abnormal type is promoted, which comprises: constructing mutant prion proteins comprising a plural number of prion proteins having been subjected to comprehensive amino acid substitutions in a prion protein, measuring the conversion ability of these mutant prion proteins from a normal type into an abnormal type, and selecting a mutant prion protein having a promoted conversion ability of a prion protein from a normal type into an abnormal type compared with the wild type prion protein.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2007/052342 WO2008099451A1 (en) | 2007-02-09 | 2007-02-09 | Method of designing high-functional protein by comprehensive amino acid substitution |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2007/052342 WO2008099451A1 (en) | 2007-02-09 | 2007-02-09 | Method of designing high-functional protein by comprehensive amino acid substitution |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2008099451A1 true WO2008099451A1 (en) | 2008-08-21 |
Family
ID=39689711
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2007/052342 WO2008099451A1 (en) | 2007-02-09 | 2007-02-09 | Method of designing high-functional protein by comprehensive amino acid substitution |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2008099451A1 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998036059A2 (en) * | 1997-02-14 | 1998-08-20 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Prion propagation inhibition by dominant-negative prion protein mutants |
JPH1156365A (en) * | 1997-08-21 | 1999-03-02 | Seibutsu Bunshi Kogaku Kenkyusho:Kk | Variant kexstatin |
WO2001027152A1 (en) * | 1999-10-15 | 2001-04-19 | Isis Innovation Limited | Method of altering polypeptide aggregation |
WO2001042453A1 (en) * | 1999-12-06 | 2001-06-14 | Biomolecular Engineering Research Institute | Structural coordinate and nmr chemical shift of protein and utilization thereof |
WO2004007546A1 (en) * | 2002-07-11 | 2004-01-22 | Eidgenoessische Technische Hochschule Zuerich | Mutant proteins and use thereof for the manufacture of medicaments and the treatment of humans or animals suffering from conformational diseases |
-
2007
- 2007-02-09 WO PCT/JP2007/052342 patent/WO2008099451A1/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998036059A2 (en) * | 1997-02-14 | 1998-08-20 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Prion propagation inhibition by dominant-negative prion protein mutants |
JPH1156365A (en) * | 1997-08-21 | 1999-03-02 | Seibutsu Bunshi Kogaku Kenkyusho:Kk | Variant kexstatin |
WO2001027152A1 (en) * | 1999-10-15 | 2001-04-19 | Isis Innovation Limited | Method of altering polypeptide aggregation |
WO2001042453A1 (en) * | 1999-12-06 | 2001-06-14 | Biomolecular Engineering Research Institute | Structural coordinate and nmr chemical shift of protein and utilization thereof |
WO2004007546A1 (en) * | 2002-07-11 | 2004-01-22 | Eidgenoessische Technische Hochschule Zuerich | Mutant proteins and use thereof for the manufacture of medicaments and the treatment of humans or animals suffering from conformational diseases |
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