WO2008099451A1 - Method of designing high-functional protein by comprehensive amino acid substitution - Google Patents

Method of designing high-functional protein by comprehensive amino acid substitution Download PDF

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Publication number
WO2008099451A1
WO2008099451A1 PCT/JP2007/052342 JP2007052342W WO2008099451A1 WO 2008099451 A1 WO2008099451 A1 WO 2008099451A1 JP 2007052342 W JP2007052342 W JP 2007052342W WO 2008099451 A1 WO2008099451 A1 WO 2008099451A1
Authority
WO
WIPO (PCT)
Prior art keywords
prion
prion protein
amino acid
type
acid substitution
Prior art date
Application number
PCT/JP2007/052342
Other languages
French (fr)
Japanese (ja)
Inventor
Tetsuyuki Kitamoto
Atsushi Kobayashi
Masahiro Asano
Original Assignee
Central Institute For Experimental Animals
Tohoku University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Central Institute For Experimental Animals, Tohoku University filed Critical Central Institute For Experimental Animals
Priority to PCT/JP2007/052342 priority Critical patent/WO2008099451A1/en
Publication of WO2008099451A1 publication Critical patent/WO2008099451A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2790/00—Viroids or subviral agents
    • C12N2790/00011—Details
    • C12N2790/10011—Prions
    • C12N2790/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

It is intended to design a prion protein molecule, which is easily converted into an abnormal prion, by transferring a point mutation into a prion protein. Namely, a method of designing a prion protein having an amino acid substitution, whereby the conversion ability of a prion protein from a normal type into an abnormal type is promoted, which comprises: constructing mutant prion proteins comprising a plural number of prion proteins having been subjected to comprehensive amino acid substitutions in a prion protein, measuring the conversion ability of these mutant prion proteins from a normal type into an abnormal type, and selecting a mutant prion protein having a promoted conversion ability of a prion protein from a normal type into an abnormal type compared with the wild type prion protein.
PCT/JP2007/052342 2007-02-09 2007-02-09 Method of designing high-functional protein by comprehensive amino acid substitution WO2008099451A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/052342 WO2008099451A1 (en) 2007-02-09 2007-02-09 Method of designing high-functional protein by comprehensive amino acid substitution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/052342 WO2008099451A1 (en) 2007-02-09 2007-02-09 Method of designing high-functional protein by comprehensive amino acid substitution

Publications (1)

Publication Number Publication Date
WO2008099451A1 true WO2008099451A1 (en) 2008-08-21

Family

ID=39689711

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/052342 WO2008099451A1 (en) 2007-02-09 2007-02-09 Method of designing high-functional protein by comprehensive amino acid substitution

Country Status (1)

Country Link
WO (1) WO2008099451A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998036059A2 (en) * 1997-02-14 1998-08-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Prion propagation inhibition by dominant-negative prion protein mutants
JPH1156365A (en) * 1997-08-21 1999-03-02 Seibutsu Bunshi Kogaku Kenkyusho:Kk Variant kexstatin
WO2001027152A1 (en) * 1999-10-15 2001-04-19 Isis Innovation Limited Method of altering polypeptide aggregation
WO2001042453A1 (en) * 1999-12-06 2001-06-14 Biomolecular Engineering Research Institute Structural coordinate and nmr chemical shift of protein and utilization thereof
WO2004007546A1 (en) * 2002-07-11 2004-01-22 Eidgenoessische Technische Hochschule Zuerich Mutant proteins and use thereof for the manufacture of medicaments and the treatment of humans or animals suffering from conformational diseases

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998036059A2 (en) * 1997-02-14 1998-08-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Prion propagation inhibition by dominant-negative prion protein mutants
JPH1156365A (en) * 1997-08-21 1999-03-02 Seibutsu Bunshi Kogaku Kenkyusho:Kk Variant kexstatin
WO2001027152A1 (en) * 1999-10-15 2001-04-19 Isis Innovation Limited Method of altering polypeptide aggregation
WO2001042453A1 (en) * 1999-12-06 2001-06-14 Biomolecular Engineering Research Institute Structural coordinate and nmr chemical shift of protein and utilization thereof
WO2004007546A1 (en) * 2002-07-11 2004-01-22 Eidgenoessische Technische Hochschule Zuerich Mutant proteins and use thereof for the manufacture of medicaments and the treatment of humans or animals suffering from conformational diseases

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