WO2008068589A3 - Neural stem cell medium and differentiation method - Google Patents

Neural stem cell medium and differentiation method Download PDF

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Publication number
WO2008068589A3
WO2008068589A3 PCT/IB2007/003748 IB2007003748W WO2008068589A3 WO 2008068589 A3 WO2008068589 A3 WO 2008068589A3 IB 2007003748 W IB2007003748 W IB 2007003748W WO 2008068589 A3 WO2008068589 A3 WO 2008068589A3
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WO
WIPO (PCT)
Prior art keywords
neural stem
stem cell
negative
cell medium
differentiation method
Prior art date
Application number
PCT/IB2007/003748
Other languages
French (fr)
Other versions
WO2008068589A2 (en
Inventor
Elena Cattaneo
Luciano Conti
Erika Reitano
Donato Goffredo
Original Assignee
Dialectica S R L
Elena Cattaneo
Luciano Conti
Erika Reitano
Donato Goffredo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP06025095A external-priority patent/EP1930412A1/en
Application filed by Dialectica S R L, Elena Cattaneo, Luciano Conti, Erika Reitano, Donato Goffredo filed Critical Dialectica S R L
Publication of WO2008068589A2 publication Critical patent/WO2008068589A2/en
Publication of WO2008068589A3 publication Critical patent/WO2008068589A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Neural stem cells are differentiated to form neurons using a medium containing a combination of a neurotrophin, an FGF receptor agonist and a glial differentiation inhibitor. Neural stem cells are preconditioned prior to transplantation so that the preconditioned cells have a marker profile which is nestin negative, Tuj1 positive, doublecortin positive, N-CAM positive, ki67 negative and phosphohistone (3) negative.
PCT/IB2007/003748 2006-12-05 2007-12-04 Neural stem cell medium and differentiation method WO2008068589A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP06025095A EP1930412A1 (en) 2006-12-05 2006-12-05 Neural stem cell medium and differentiation method
EP06025095.8 2006-12-05
EP07005026.5 2007-03-12
EP07005026 2007-03-12

Publications (2)

Publication Number Publication Date
WO2008068589A2 WO2008068589A2 (en) 2008-06-12
WO2008068589A3 true WO2008068589A3 (en) 2008-09-04

Family

ID=39365914

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2007/003748 WO2008068589A2 (en) 2006-12-05 2007-12-04 Neural stem cell medium and differentiation method

Country Status (1)

Country Link
WO (1) WO2008068589A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010147803A2 (en) * 2009-06-08 2010-12-23 Regents Of The University Of Michigan Adult cerebellum-derived neural stem cells and compositions and methods for producing oligodendrocytes
EP2970897B1 (en) 2013-03-14 2019-11-27 The Regents of The University of California In vitro production of medial ganglionic eminence precursor cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005121318A2 (en) * 2004-06-09 2005-12-22 The University Court Of The University Of Edinburgh Neural stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005121318A2 (en) * 2004-06-09 2005-12-22 The University Court Of The University Of Edinburgh Neural stem cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHOI ET AL: "Analysis of neuron-like differentiation of human bone marrow mesenchymal stem cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 350, no. 1, 10 November 2006 (2006-11-10), pages 138 - 146, XP005676306, ISSN: 0006-291X *
CONTI L ET AL: "Niche-independent symmetrical self-renewal of a mammalian tissue stem cell", PLOS BIOLOGY, vol. 3, no. 9, September 2005 (2005-09-01), pages 1594 - 1606, XP002350601, ISSN: 1544-9173 *
CONTI L ET AL: "Protocol S1 Derivation and manipulation of NS cell lines", PLOS BIOLOGY, September 2005 (2005-09-01), pages 1 - 5, XP002431294, Retrieved from the Internet <URL:http://biology.plosjournals.org/archive/1545-7885/3/9/supinfo/10.1371_journal.pbio.0030283.sd001.doc> [retrieved on 20070426] *
CONTI LUCIANO ET AL: "Neural stem cell systems: diversities and properties after transplantation in animal models of diseases.", BRAIN PATHOLOGY (ZURICH, SWITZERLAND) APR 2006, vol. 16, no. 2, April 2006 (2006-04-01), pages 143 - 154, XP002431136, ISSN: 1015-6305 *
HSIEH J ET AL: "Epigenetic control of neural stem cell fate", CURRENT OPINION IN GENETICS & DEVELOPMENT, CURRENT BIOLOGY LTD, vol. 14, no. 5, October 2004 (2004-10-01), pages 461 - 469, XP004566197, ISSN: 0959-437X *
PALMER T D ET AL: "THE ADULT RAT HIPPOCAMPUS CONTAINS PRIMORDIAL NEURAL STEM CELLS", MOLECULAR AND CELLULAR NEUROSCIENCES, SAN DIEGO, CA, US, vol. 8, no. 6, 1997, pages 389 - 404, XP002034682 *
RAO ET AL: "Fetal hippocampal CA3 cell grafts enriched with FGF-2 and BDNF exhibit robust long-term survival and integration and suppress aberrant mossy fiber sprouting in the injured middle-aged hippocampus", NEUROBIOLOGY OF DISEASE, BLACKWELL SCIENCE, OXFORD, GB, vol. 21, no. 2, February 2006 (2006-02-01), pages 276 - 290, XP005243140, ISSN: 0969-9961 *

Also Published As

Publication number Publication date
WO2008068589A2 (en) 2008-06-12

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