WO2008062310A3 - Elimination of the bacteria that cause pierce's disease - Google Patents
Elimination of the bacteria that cause pierce's disease Download PDFInfo
- Publication number
- WO2008062310A3 WO2008062310A3 PCT/IB2007/004184 IB2007004184W WO2008062310A3 WO 2008062310 A3 WO2008062310 A3 WO 2008062310A3 IB 2007004184 W IB2007004184 W IB 2007004184W WO 2008062310 A3 WO2008062310 A3 WO 2008062310A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- bacteria
- culture
- fastidiosa
- eliminates
- disease
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
- C07K14/4354—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from nematodes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Pest Control & Pesticides (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agronomy & Crop Science (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
XyIeIIa fastidiosa is the cause of the Pierce's Disease in grapevines and other cultures of economic importance that are susceptible to the bacteria. Anti-Xylella fastidiosa substances were developed comprising hen scFv antibodies attached to a lytic peptide Cecropin Pl (CPI), a derivative of a nematode, expressed in M13 phages or in soluble form. The recombinant substance specifically eliminates 100% of the XyIeIIa fastidiosa in culture while using a concentration of 3.6 x IO(5) phages/bacterial in 28 hours of incubation. A scFv expressed in M13 phage eliminates 100% of the bacteria in a culture between 28 and 48 hours. A soluble scFv by itself or attached to the lytic peptide CP1 eliminates almost 100% of the XyIeIIa fastidiosa bacteria in culture. This strategy has a potential application to solve diseases in plants caused by bacteria.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MXNL06000436 | 2006-05-19 | ||
MXNL/2006/000436 | 2006-05-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008062310A2 WO2008062310A2 (en) | 2008-05-29 |
WO2008062310A3 true WO2008062310A3 (en) | 2009-04-23 |
Family
ID=39430117
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2007/004184 WO2008062310A2 (en) | 2006-05-19 | 2007-05-18 | Elimination of the bacteria that cause pierce's disease |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2008062310A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102093998B (en) * | 2010-12-09 | 2012-11-07 | 山东大学 | Preparation method of antibacterial peptide cecropin feed additive |
US10499651B2 (en) | 2012-10-19 | 2019-12-10 | The Texas A&M University System | Method for treatment and control of plant disease |
ES2774291T3 (en) * | 2012-10-19 | 2020-07-20 | Texas A & M Univ Sys | Methods and compositions for the treatment and control of plant diseases |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030182686A1 (en) * | 1996-06-26 | 2003-09-25 | Ralph Scorza | Disease resistance in Vitis |
US20040208873A1 (en) * | 2002-12-16 | 2004-10-21 | Medarex, Inc. | Human monoclonal antibodies against interleukin 8 (IL-8) |
US6825325B1 (en) * | 1998-10-16 | 2004-11-30 | Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Molecular pathogenicide mediated plant disease resistance |
-
2007
- 2007-05-18 WO PCT/IB2007/004184 patent/WO2008062310A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030182686A1 (en) * | 1996-06-26 | 2003-09-25 | Ralph Scorza | Disease resistance in Vitis |
US6825325B1 (en) * | 1998-10-16 | 2004-11-30 | Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Molecular pathogenicide mediated plant disease resistance |
US20040208873A1 (en) * | 2002-12-16 | 2004-10-21 | Medarex, Inc. | Human monoclonal antibodies against interleukin 8 (IL-8) |
Non-Patent Citations (1)
Title |
---|
PILLAI ET AL.: "Cecropin P1 and novel nematode cecropins: a bacteria-inducible antimicrobial peptide family in the nematode Ascaris suum.", BIOCHEM. J., vol. 390, 2005, pages 207 - 214 * |
Also Published As
Publication number | Publication date |
---|---|
WO2008062310A2 (en) | 2008-05-29 |
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