WO2008038296A3 - Recombinant calcitonin fused to interleukin-2 - Google Patents

Recombinant calcitonin fused to interleukin-2 Download PDF

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Publication number
WO2008038296A3
WO2008038296A3 PCT/IN2007/000252 IN2007000252W WO2008038296A3 WO 2008038296 A3 WO2008038296 A3 WO 2008038296A3 IN 2007000252 W IN2007000252 W IN 2007000252W WO 2008038296 A3 WO2008038296 A3 WO 2008038296A3
Authority
WO
WIPO (PCT)
Prior art keywords
interleukin
calcitonin
fusion proteins
compositions
fused
Prior art date
Application number
PCT/IN2007/000252
Other languages
French (fr)
Other versions
WO2008038296A2 (en
Inventor
Shivraj Livy
Krishna Chand Gadiraju Sri
Ramana Kondiboyina Venkata
Original Assignee
Reliance Life Sciences Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reliance Life Sciences Pvt Ltd filed Critical Reliance Life Sciences Pvt Ltd
Publication of WO2008038296A2 publication Critical patent/WO2008038296A2/en
Publication of WO2008038296A3 publication Critical patent/WO2008038296A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention provides fusion proteins of Calcitonin with Interleukin 2 that have enhanced biological activity compared to Calcitonin. In particular, the present invention provides the process of preparing and using compositions of the fusion protein of Calcitonin with Interleukin 2. Also provided are calcitonin prodrugs and fusion proteins comprising IL2 and calcitonin that are designed to cleave and release calcitonin and/or interleukin-2 on administration and uses thereof. Compositions for treating cancer comprising said fusion proteins are provided.
PCT/IN2007/000252 2006-06-21 2007-06-21 Recombinant calcitonin fused to interleukin-2 WO2008038296A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN973/MUM/2006 2006-06-21
IN973MU2006 2006-06-21

Publications (2)

Publication Number Publication Date
WO2008038296A2 WO2008038296A2 (en) 2008-04-03
WO2008038296A3 true WO2008038296A3 (en) 2008-10-09

Family

ID=39230683

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2007/000252 WO2008038296A2 (en) 2006-06-21 2007-06-21 Recombinant calcitonin fused to interleukin-2

Country Status (1)

Country Link
WO (1) WO2008038296A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230002442A1 (en) * 2020-01-03 2023-01-05 The Texas A&M University System An activated cysteine-directed polypeptide ligation technique
CN114591991B (en) * 2022-03-31 2023-05-09 西南交通大学 Method for preparing calcipotriol key chiral intermediate based on short-chain carbonyl reductase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997029127A1 (en) * 1996-02-06 1997-08-14 Bionebraska, Inc. Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
CA2540446A1 (en) * 2003-09-30 2005-04-07 Daiichi Asubio Pharma Co., Ltd. Polypeptide cleavage method using ompt protease variant
GB2415904A (en) * 2005-02-07 2006-01-11 Chiron Corp Interleukin-2 composition

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997029127A1 (en) * 1996-02-06 1997-08-14 Bionebraska, Inc. Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
CA2540446A1 (en) * 2003-09-30 2005-04-07 Daiichi Asubio Pharma Co., Ltd. Polypeptide cleavage method using ompt protease variant
GB2415904A (en) * 2005-02-07 2006-01-11 Chiron Corp Interleukin-2 composition

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CELLULAR AND MOLECULAR NEUROBIOLOGY APR 1994, vol. 14, no. 2, April 1994 (1994-04-01), pages 175 - 184, ISSN: 0272-4340 *
CHA H J ET AL: "Purification of human interleukin-2 fusion protein produced in insect larvae is facilitated by fusion with green fluorescent protein and metal affinity ligand.", BIOTECHNOLOGY PROGRESS 1999 MAR-APR, vol. 15, no. 2, March 1999 (1999-03-01), pages 283 - 286, XP002121721, ISSN: 8756-7938 *
DATABASE Geneseq [online] 7 April 2005 (2005-04-07), "Human albumin fusion-related interleukin-2 (IL-2) mutant DNA - SEQ 529.", XP002488401, retrieved from EBI accession no. GSN:ADW45525 Database accession no. ADW45525 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; April 1994 (1994-04-01), SAWADA M ET AL: "Inhibition of the calcitonin-induced outward current in identified Aplysia neurons by interleukin-1 and interleukin-2.", XP002488402, Database accession no. NLM7842475 *
MATTHEWS J L: "Effect of calcitonin on bone cell ultrastructure", BONE AND MINERAL 1992 NL, vol. 16, no. 3, 1992, pages 178 - 181, XP002488399, ISSN: 0169-6009 *
RIES W L ET AL: "Interleukin-2 stimulates osteoclastic activity: Increased acid production and radioactive calcium release", JOURNAL OF PERIODONTAL RESEARCH 1989 DK, vol. 24, no. 4, 1989, pages 242 - 246, XP002488398, ISSN: 0022-3484 *
WANG F ET AL: "Calcitonin gene-related peptide inhibits interleukin 2 production by murine T lymphocytes.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 15 OCT 1992, vol. 267, no. 29, 15 October 1992 (1992-10-15), pages 21052 - 21057, XP002488400, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2008038296A2 (en) 2008-04-03

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