WO2008024319A8 - Microfluidic devices for nucleic acid assembly - Google Patents

Microfluidic devices for nucleic acid assembly

Info

Publication number
WO2008024319A8
WO2008024319A8 PCT/US2007/018437 US2007018437W WO2008024319A8 WO 2008024319 A8 WO2008024319 A8 WO 2008024319A8 US 2007018437 W US2007018437 W US 2007018437W WO 2008024319 A8 WO2008024319 A8 WO 2008024319A8
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
microfluidic devices
reagents
acid assembly
devices
Prior art date
Application number
PCT/US2007/018437
Other languages
French (fr)
Other versions
WO2008024319A3 (en
WO2008024319A2 (en
Inventor
Craig Douglas Muir
Christopher J Emig
Original Assignee
Codon Devices Inc
Craig Douglas Muir
Christopher J Emig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codon Devices Inc, Craig Douglas Muir, Christopher J Emig filed Critical Codon Devices Inc
Publication of WO2008024319A2 publication Critical patent/WO2008024319A2/en
Publication of WO2008024319A3 publication Critical patent/WO2008024319A3/en
Publication of WO2008024319A8 publication Critical patent/WO2008024319A8/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00423—Means for dispensing and evacuation of reagents using filtration, e.g. through porous frits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00905—Separation
    • B01J2219/00912—Separation by electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Certain aspects of the present invention provide devices and methods for assembling nucleic acid molecules on micro fluidic devices, e.g. via extension and/or ligation reactions. The invention also provides systems comprising one or more acoustic liquid handlers, one or more microfluidic devices, and/or one or more robotic liquid handlers for automating a nucleic acid assembly procedure. The microdevice may comprise the following components: a sipper, or means for transferring reagents (310); a main channel (315); a spitter, or means for transferring reagents (320), sample wells for storage of reagents or buffers (330, 335, 340), separation or concentration channels (350, 355) and sample or reaction wells (360, 365) e.g. a thermocycler.
PCT/US2007/018437 2006-08-20 2007-08-20 Microfluidic devices for nucleic acid assembly WO2008024319A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US83896006P 2006-08-20 2006-08-20
US60/838,960 2006-08-20
US87833206P 2006-12-31 2006-12-31
US87833006P 2006-12-31 2006-12-31
US60/878,330 2006-12-31
US60/878,332 2006-12-31

Publications (3)

Publication Number Publication Date
WO2008024319A2 WO2008024319A2 (en) 2008-02-28
WO2008024319A3 WO2008024319A3 (en) 2008-04-10
WO2008024319A8 true WO2008024319A8 (en) 2008-05-29

Family

ID=38996695

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/018437 WO2008024319A2 (en) 2006-08-20 2007-08-20 Microfluidic devices for nucleic acid assembly

Country Status (1)

Country Link
WO (1) WO2008024319A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023601B2 (en) 2002-09-12 2015-05-05 Gen9, Inc. Microarray synthesis and assembly of gene-length polynucleotides

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102759466A (en) 2004-09-15 2012-10-31 英特基因有限公司 Microfluidic devices
CA2641271A1 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US20110039303A1 (en) 2007-02-05 2011-02-17 Stevan Bogdan Jovanovich Microfluidic and nanofluidic devices, systems, and applications
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
GB0815472D0 (en) * 2008-08-22 2008-10-01 Iti Scotland Ltd Microfluidic system
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
WO2010077322A1 (en) 2008-12-31 2010-07-08 Microchip Biotechnologies, Inc. Instrument with microfluidic chip
WO2010141326A1 (en) 2009-06-02 2010-12-09 Integenx Inc. Fluidic devices with diaphragm valves
SG176669A1 (en) 2009-06-05 2012-01-30 Integenx Inc Universal sample preparation system and use in an integrated analysis system
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP3597771A1 (en) 2009-11-25 2020-01-22 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (en) 2010-11-12 2017-03-29 Gen9股份有限公司 The method and apparatus of nucleic acid synthesis
US10131903B2 (en) * 2011-04-01 2018-11-20 The Regents Of The University Of California Microfluidic platform for synthetic biology applications
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
LT2944693T (en) 2011-08-26 2019-08-26 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
LT2841601T (en) 2012-04-24 2019-07-10 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
EP3483311A1 (en) 2012-06-25 2019-05-15 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
EP4219706A1 (en) * 2012-08-16 2023-08-02 Synthetic Genomics, Inc. Automated dsdna synthesis using a digital to biological converter
CN110560187B (en) 2013-11-18 2022-01-11 尹特根埃克斯有限公司 Cartridge and instrument for sample analysis
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
US10690627B2 (en) 2014-10-22 2020-06-23 IntegenX, Inc. Systems and methods for sample preparation, processing and analysis
EP3366370A1 (en) 2017-02-22 2018-08-29 Briefcase Biotec GmbH Device for the synthesis of oligonucleotides
WO2019074512A1 (en) * 2017-10-12 2019-04-18 Hewlett-Packard Development Company, L.P. Embedded microfluidic devices
US11249100B2 (en) 2018-01-05 2022-02-15 Worcester Polytechnic Institute Modular robotic systems for delivering fluid to microfluidic devices
US10046322B1 (en) 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
WO2020018074A1 (en) 2018-07-17 2020-01-23 Hewlett-Packard Development Company, L.P. Droplet ejectors to provide fluids to droplet ejectors
WO2020018073A1 (en) 2018-07-17 2020-01-23 Hewlett-Packard Development Company, L.P. Droplet ejectors with target media
WO2019209273A1 (en) 2018-04-24 2019-10-31 Hewlett-Packard Development Company, L.P. Microfluidic devices
WO2019209374A1 (en) 2018-04-24 2019-10-31 Hewlett-Packard Development Company, L.P. Sequenced droplet ejection to deliver fluids
WO2020264260A1 (en) * 2019-06-27 2020-12-30 Zymergen Inc. Laboratory automation system implementing efficient path for material and labware transfers
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
WO1998045481A1 (en) * 1997-04-04 1998-10-15 Caliper Technologies Corporation Closed-loop biochemical analyzers
US6372484B1 (en) * 1999-01-25 2002-04-16 E.I. Dupont De Nemours And Company Apparatus for integrated polymerase chain reaction and capillary electrophoresis
US6613581B1 (en) * 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US7179423B2 (en) * 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7419784B2 (en) * 2002-04-02 2008-09-02 Dubrow Robert S Methods, systems and apparatus for separation and isolation of one or more sample components of a sample biological material
CA2501857A1 (en) * 2002-10-09 2004-04-22 The Board Of Trustees Of The University Of Illinois Microfluidic systems and components
JP2005319407A (en) * 2004-05-10 2005-11-17 Hitachi Ltd Instrument using piezoelectric device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023601B2 (en) 2002-09-12 2015-05-05 Gen9, Inc. Microarray synthesis and assembly of gene-length polynucleotides

Also Published As

Publication number Publication date
WO2008024319A3 (en) 2008-04-10
WO2008024319A2 (en) 2008-02-28

Similar Documents

Publication Publication Date Title
WO2008024319A8 (en) Microfluidic devices for nucleic acid assembly
WO2007120241A3 (en) Droplet-based biochemistry
Matosevic et al. Fundamentals and applications of immobilized microfluidic enzymatic reactors
WO2008063227A3 (en) Microfluidic devices
CA2756463C (en) Slip chip device and methods
US8748165B2 (en) Methods for generating short tandem repeat (STR) profiles
Kodzius et al. Inhibitory effect of common microfluidic materials on PCR outcome
WO2004058406A3 (en) Microfluidics devices and methods for diluting samples and reagents
WO2007081385A8 (en) Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2005068628A3 (en) Methods for nucleic acid isolation and kits using solid phase material
WO2001034302A3 (en) Biochannel assay for hybridization with biomaterial
WO2006124458A3 (en) Method and device for conducting biochemical or chemical reactions at multiple temperatures
AU5887000A (en) Devices and methods for the performance of miniaturized in vitro amplification assays
WO2007021813A3 (en) Microfluidic system and methods
WO2006127191A3 (en) Analysis engine and database for manipulating parameters for fluidic systems on a chip
CA2284779A1 (en) Controlled fluid transport in microfabricated polymeric substrates
WO2008030433A3 (en) Chip and cartridge design configuration for performing micro-fluidic assays
WO2008097559A3 (en) Manipulation of fluids and reactions in microfluidic systems
WO2006071470A3 (en) Microfluidic devices with chemical reaction circuits
US20160001284A1 (en) Fluidic Interfacing System and Assembly
AU2017202597A1 (en) Slip chip device and methods
Mathies et al. Fluid control structures in microfluidic devices
SG142188A1 (en) Lab-on-cd systems with magnetically actuated micro check valves and/or magnetic immobilization
Peeters et al. MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION (MLPA) ON-CHIP
SHOJI Nanotechnology and Micro System Technology for Laboratory Test

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07811442

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

NENP Non-entry into the national phase in:

Ref country code: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07811442

Country of ref document: EP

Kind code of ref document: A2