WO2008007082A3 - Cell growth medium - Google Patents

Cell growth medium Download PDF

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Publication number
WO2008007082A3
WO2008007082A3 PCT/GB2007/002584 GB2007002584W WO2008007082A3 WO 2008007082 A3 WO2008007082 A3 WO 2008007082A3 GB 2007002584 W GB2007002584 W GB 2007002584W WO 2008007082 A3 WO2008007082 A3 WO 2008007082A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell growth
growth medium
feeder
stem cells
relates
Prior art date
Application number
PCT/GB2007/002584
Other languages
French (fr)
Other versions
WO2008007082A2 (en
Inventor
Miho Furue
Peter Andrews
Tetsuji Okamoto
Denry J Sato
Original Assignee
Univ Sheffield
Miho Furue
Peter Andrews
Tetsuji Okamoto
Denry J Sato
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sheffield, Miho Furue, Peter Andrews, Tetsuji Okamoto, Denry J Sato filed Critical Univ Sheffield
Priority to EP07766180A priority Critical patent/EP2038404A2/en
Priority to CA002657539A priority patent/CA2657539A1/en
Priority to AU2007274065A priority patent/AU2007274065A1/en
Priority to JP2009518958A priority patent/JP5227318B2/en
Publication of WO2008007082A2 publication Critical patent/WO2008007082A2/en
Publication of WO2008007082A3 publication Critical patent/WO2008007082A3/en
Priority to GB0823575A priority patent/GB2452456A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Abstract

The invention relates to a method to culture primate embryonic stem cells in feeder and serum free conditions.
PCT/GB2007/002584 2006-07-12 2007-07-10 Cell growth medium WO2008007082A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP07766180A EP2038404A2 (en) 2006-07-12 2007-07-10 Cell growth medium
CA002657539A CA2657539A1 (en) 2006-07-12 2007-07-10 Cell growth medium
AU2007274065A AU2007274065A1 (en) 2006-07-12 2007-07-10 Cell growth medium
JP2009518958A JP5227318B2 (en) 2006-07-12 2007-07-10 Cell growth medium
GB0823575A GB2452456A (en) 2006-07-12 2008-12-29 Cell growth medium

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0613756.6 2006-07-12
GBGB0613756.6A GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium

Publications (2)

Publication Number Publication Date
WO2008007082A2 WO2008007082A2 (en) 2008-01-17
WO2008007082A3 true WO2008007082A3 (en) 2008-03-06

Family

ID=36955441

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/002584 WO2008007082A2 (en) 2006-07-12 2007-07-10 Cell growth medium

Country Status (7)

Country Link
EP (1) EP2038404A2 (en)
JP (1) JP5227318B2 (en)
CN (1) CN101490243A (en)
AU (1) AU2007274065A1 (en)
CA (1) CA2657539A1 (en)
GB (2) GB0613756D0 (en)
WO (1) WO2008007082A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells

Families Citing this family (30)

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Publication number Priority date Publication date Assignee Title
DK3441459T3 (en) 2006-08-02 2021-06-07 Technion Res & Dev Foundation PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE
US9688956B2 (en) 2008-06-05 2017-06-27 National Cheng Kung University Method for preserving proliferation and differentiation potential of mesenchymal stem cells
CA2765670C (en) 2008-06-24 2018-05-15 Bioactive Surgical, Inc. Surgical sutures incorporated with stem cells or other bioactive materials
KR20110042107A (en) 2008-08-07 2011-04-22 바이오엑티브 써지컬, 아이엔씨. Stem cell capture and immobilization coatings for medical devices and implants
CA2747398C (en) * 2008-12-17 2023-06-20 The Scripps Research Institute Generation and maintenance of stem cells
EP2456461A1 (en) * 2009-07-21 2012-05-30 Transgene SA Enzymatic composition for the digestion of chicken embryos
CN101812480B (en) * 2009-11-16 2013-04-10 西北农林科技大学 Method utilizing transcription factor to transfect bovine body cell into induced pluripotent stem cell
JP5714267B2 (en) * 2010-08-26 2015-05-07 日本メナード化粧品株式会社 Stem cell undifferentiation maintenance agent and proliferation promoter
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
CN102586176B (en) * 2012-01-11 2013-05-08 中国科学院生物物理研究所 Novel animal source-free and feed layer-free human pluripotent stem cell culture system
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
KR102211266B1 (en) 2013-01-31 2021-02-04 아지노모토 가부시키가이샤 Culture method for stable undifferentiated proliferation of pluripotent stem cells
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
US10066211B2 (en) * 2013-06-28 2018-09-04 Kaneka Corporation Method for screening for pluripotent stem cell growth-promoting factor
WO2015042356A1 (en) * 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CN104357379B (en) * 2014-09-30 2017-08-08 刘兴宇 Stem cell media
CN106032527B (en) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 It is a kind of tolerance low-density without feeder layer human pluripotent stem cells culture medium
CN105907705B (en) * 2016-06-28 2020-01-24 广州市搏克生物技术有限公司 Pluripotent stem cell culture medium
JP7049347B2 (en) * 2017-01-23 2022-04-06 ステムセル テクノロジーズ カナダ インコーポレイテッド Mediums and Methods to Enhance Stem Cell Survival and Proliferation
CN106754715A (en) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 A kind of trophoblastic preparation method for candidate stem cell culture
CN106754652B (en) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 IPS cell differentiation at ectoderm progenitor cells serum-free induced medium and abductive approach
CN106754657B (en) * 2017-03-28 2022-07-22 北京赛斯达生物技术有限公司 Serum-free medium for monkey embryonic stem cells
EP3436568B1 (en) * 2017-05-31 2023-06-14 PromoCell GmbH Culture medium for pluripotent stem cells
CN110157662A (en) * 2018-02-15 2019-08-23 郭永珍 A kind of cell additive and preparation method thereof
KR102147780B1 (en) * 2018-03-16 2020-08-25 사회복지법인 삼성생명공익재단 Culture medium composition for promoting the proliferation of stem cell comprising FGF-17 as an effective ingredient and method of culturing stem cells using the same
EP3816279A4 (en) * 2018-06-27 2022-04-20 Ajinomoto Co., Inc. Additive for culturing stem cells, culturing medium, and culturing method
CN110684716A (en) * 2018-07-08 2020-01-14 郭永珍 Preparation method of cell additive and product thereof
CN109486766B (en) * 2018-11-26 2021-05-07 中山大学 Lacrimal gland stem cell, culture system and culture method of lacrimal gland stem cell
CN111484970B (en) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 Serum-free and feeder-layer-free embryo and pluripotent stem cell culture medium with low protein content
WO2023150293A2 (en) * 2022-02-03 2023-08-10 Steakholder Foods Ltd. Accelerated myotube formation

Citations (6)

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WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
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WO2006029197A1 (en) * 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
WO2006036925A1 (en) * 2004-09-28 2006-04-06 Wicell Research Institute, Inc. Cultivation of primate embryonic stem cells

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ATE466073T1 (en) * 2003-12-26 2010-05-15 Makoto Asashima BASAL MEDIUM FOR CULTIVATION OF ES CELLS

Patent Citations (6)

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WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
WO2006017370A2 (en) * 2004-07-13 2006-02-16 Geron Corporation Medium for growing human embryonic stem cells
WO2006029197A1 (en) * 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
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Non-Patent Citations (4)

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LI Y ET AL: "Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 91, no. 6, 20 September 2005 (2005-09-20), pages 688 - 698, XP002351170, ISSN: 0006-3592 *
REN WENWEN ET AL: "bFGF and heparin but not laminin are necessary factors in the mediums that affect NSCs differentiation into cholinergic neurons.", NEUROLOGICAL RESEARCH JAN 2006, vol. 28, no. 1, January 2006 (2006-01-01), pages 87 - 90, XP002462651, ISSN: 0161-6412 *
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
JP7053551B2 (en) 2009-11-12 2022-04-12 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド Mediums, cell cultures and methods for culturing pluripotent stem cells in an undifferentiated state

Also Published As

Publication number Publication date
CA2657539A1 (en) 2008-01-17
WO2008007082A2 (en) 2008-01-17
JP5227318B2 (en) 2013-07-03
CN101490243A (en) 2009-07-22
GB0823575D0 (en) 2009-01-28
EP2038404A2 (en) 2009-03-25
JP2009542247A (en) 2009-12-03
GB0613756D0 (en) 2006-08-23
AU2007274065A1 (en) 2008-01-17
GB2452456A (en) 2009-03-04

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