WO2008005459A3 - Methods for fragmentation and labeling of nucleic acids - Google Patents
Methods for fragmentation and labeling of nucleic acids Download PDFInfo
- Publication number
- WO2008005459A3 WO2008005459A3 PCT/US2007/015409 US2007015409W WO2008005459A3 WO 2008005459 A3 WO2008005459 A3 WO 2008005459A3 US 2007015409 W US2007015409 W US 2007015409W WO 2008005459 A3 WO2008005459 A3 WO 2008005459A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- nucleic acids
- fragmentation
- labeling
- fragments
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/305,633 US20100022403A1 (en) | 2006-06-30 | 2007-07-02 | Methods for fragmentation and labeling of nucleic acids |
CA002656315A CA2656315A1 (en) | 2006-06-30 | 2007-07-02 | Methods for fragmentation and labeling of nucleic acids |
EP07810169.8A EP2038429B1 (en) | 2006-06-30 | 2007-07-02 | Methods for fragmentation and labeling of nucleic acids |
US13/411,170 US8551709B2 (en) | 2006-06-30 | 2012-03-02 | Methods for fragmentation and labeling of nucleic acids |
US14/012,409 US20140065692A1 (en) | 2006-06-30 | 2013-08-28 | Methods for Fragmentation and Labeling of Nucleic Acids |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81789006P | 2006-06-30 | 2006-06-30 | |
US60/817,890 | 2006-06-30 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/305,633 A-371-Of-International US20100022403A1 (en) | 2006-06-30 | 2007-07-02 | Methods for fragmentation and labeling of nucleic acids |
US13/411,170 Continuation US8551709B2 (en) | 2006-06-30 | 2012-03-02 | Methods for fragmentation and labeling of nucleic acids |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008005459A2 WO2008005459A2 (en) | 2008-01-10 |
WO2008005459A3 true WO2008005459A3 (en) | 2008-02-21 |
Family
ID=38895198
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2007/015409 WO2008005459A2 (en) | 2006-06-30 | 2007-07-02 | Methods for fragmentation and labeling of nucleic acids |
Country Status (4)
Country | Link |
---|---|
US (3) | US20100022403A1 (en) |
EP (1) | EP2038429B1 (en) |
CA (1) | CA2656315A1 (en) |
WO (1) | WO2008005459A2 (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7771934B2 (en) | 2000-12-13 | 2010-08-10 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7771946B2 (en) | 2001-03-09 | 2010-08-10 | Nugen Technologies, Inc. | Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US8465950B2 (en) | 2003-04-14 | 2013-06-18 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004513617A (en) * | 2000-06-26 | 2004-05-13 | ニューゲン テクノロジーズ, インコーポレイテッド | Methods and compositions for transcription-based nucleic acid amplification |
EP1573056A4 (en) * | 2002-05-17 | 2007-11-28 | Nugen Technologies Inc | Methods for fragmentation, labeling and immobilization of nucleic acids |
WO2007136717A1 (en) * | 2006-05-16 | 2007-11-29 | Nugen Technologies, Inc. | Nucleic acid separation and purification method based on reversible charge interactions |
CA2656315A1 (en) | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US8669061B2 (en) * | 2008-06-26 | 2014-03-11 | Roche Molecular Systems, Inc. | Method for the prevention of carryover contamination in nucleic acid amplification technologies |
US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
US20110224105A1 (en) * | 2009-08-12 | 2011-09-15 | Nugen Technologies, Inc. | Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid |
US20110189679A1 (en) * | 2009-09-11 | 2011-08-04 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
ES2445709T3 (en) * | 2010-12-31 | 2014-03-04 | Progenika Biopharma, S.A. | Method for the identification by molecular techniques of genetic variants that do not encode D (D-) antigen and encode altered C (C + W) antigen |
DK3037536T3 (en) | 2011-01-28 | 2020-01-13 | Illumina Inc | OLIGONUCLEOTID REPLACEMENT FOR DI-TAGGED AND DIRECTORY LIBRARIES |
WO2012106546A2 (en) | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
WO2012149042A2 (en) | 2011-04-25 | 2012-11-01 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
CN104093890B (en) | 2012-01-26 | 2016-04-20 | 纽亘技术公司 | The composition produced for the enrichment of target nucleotide sequence and efficient library and method |
CN108018343B (en) | 2012-05-10 | 2023-01-03 | 通用医疗公司 | Method for determining nucleotide sequence |
JP6181751B2 (en) | 2012-06-18 | 2017-08-16 | ニューゲン テクノロジーズ, インコーポレイテッド | Compositions and methods for negative selection of unwanted nucleic acid sequences |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
CA3094792A1 (en) | 2013-03-13 | 2014-09-18 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
US10519502B2 (en) * | 2013-10-31 | 2019-12-31 | The Children's Hospital Of Philadelphia | Mitochondrial disease genetic diagnostics |
EP3068883B1 (en) | 2013-11-13 | 2020-04-29 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
CN106414765A (en) | 2013-12-20 | 2017-02-15 | Illumina公司 | Preserving genomic connectivity information in fragmented genomic DNA samples |
CN106661612A (en) | 2014-01-27 | 2017-05-10 | 通用医疗公司 | Methods of preparing nucleic acids for sequencing |
US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
WO2015189636A1 (en) | 2014-06-13 | 2015-12-17 | Illumina Cambridge Limited | Methods and compositions for preparing sequencing libraries |
CN112430641A (en) | 2014-06-30 | 2021-03-02 | 亿明达股份有限公司 | Methods and compositions using unilateral transposition |
JP6803327B2 (en) | 2014-08-06 | 2020-12-23 | ニューゲン テクノロジーズ, インコーポレイテッド | Digital measurements from targeted sequencing |
BR112017007912A2 (en) | 2014-10-17 | 2018-01-23 | Illumina Cambridge Limited | contiguity preservation transposon |
ES2903357T3 (en) | 2016-09-15 | 2022-04-01 | Archerdx Llc | Nucleic Acid Sample Preparation Methods |
CA3037190A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
US11667951B2 (en) | 2016-10-24 | 2023-06-06 | Geneinfosec, Inc. | Concealing information present within nucleic acids |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
CN108486100A (en) * | 2018-03-22 | 2018-09-04 | 苏州泰康吉安仪器科技有限公司 | A kind of controllable fragmentation methods of DNA length and its application in building library |
EP3827011A4 (en) * | 2018-07-24 | 2022-06-22 | Salish Bioscience Inc. | Methods and composition for targeted genomic analysis |
NL2022043B1 (en) | 2018-11-21 | 2020-06-03 | Akershus Univ Hf | Tagmentation-Associated Multiplex PCR Enrichment Sequencing |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5573913A (en) * | 1994-02-28 | 1996-11-12 | Boehringer Mannheim Gmbh | 3'-RNA labelling with terminal transferase |
US20040005614A1 (en) * | 2002-05-17 | 2004-01-08 | Nurith Kurn | Methods for fragmentation, labeling and immobilization of nucleic acids |
Family Cites Families (85)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4876187A (en) * | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
US5011769A (en) * | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
US4996143A (en) * | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
US6270961B1 (en) * | 1987-04-01 | 2001-08-07 | Hyseq, Inc. | Methods and apparatus for DNA sequencing and DNA identification |
US6090591A (en) * | 1987-07-31 | 2000-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
JP2650159B2 (en) * | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | Nucleic acid amplification method |
CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
US5082830A (en) * | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
ES2091192T3 (en) * | 1989-01-05 | 1996-11-01 | Leti Lab | APPLICATION OF SPECIFIC PROPERTIES OF ALLERGENS, ALLERGENS FROM ANIMAL AND VEGETABLE SOURCES AND METHODS FOR THEIR ISOLATION. |
US5708154A (en) * | 1989-02-24 | 1998-01-13 | City Of Hope | RNA-DNA hybrid molecules of nucleic acid |
US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5683896A (en) * | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
US5035996A (en) * | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
US5143854A (en) * | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
DK0408295T3 (en) * | 1989-07-11 | 1996-09-16 | Gen Probe Inc | Methods for amplifying nucleic acid sequences |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US5391785A (en) * | 1990-01-16 | 1995-02-21 | La Jolla Pharmaceutial Company | Intermediates for providing functional groups on the 5' end of oligonucleotides |
CA2037349C (en) * | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
HU218095B (en) * | 1990-05-01 | 2000-05-28 | Amgen Inc. | Process for reducing transitional contaminations in amplification processes |
US5667976A (en) * | 1990-05-11 | 1997-09-16 | Becton Dickinson And Company | Solid supports for nucleic acid hybridization assays |
US5194370A (en) * | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
DK0540693T3 (en) * | 1990-07-24 | 1999-09-13 | Hoffmann La Roche | Reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid |
US5090591A (en) * | 1991-03-18 | 1992-02-25 | Longford Equipment International Limited | Article dispenser for use with continuous strip of articles |
ATE205542T1 (en) * | 1992-03-04 | 2001-09-15 | Univ California | COMPARATIVE GENOME HYBRIDIZATION |
US5965362A (en) * | 1992-03-04 | 1999-10-12 | The Regents Of The University Of California | Comparative genomic hybridization (CGH) |
WO1993018176A1 (en) * | 1992-03-11 | 1993-09-16 | Dana-Farber Cancer Institute | METHODS TO CLONE mRNA |
CA2135073C (en) * | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
US6172208B1 (en) * | 1992-07-06 | 2001-01-09 | Genzyme Corporation | Oligonucleotides modified with conjugate groups |
DK0652973T3 (en) * | 1992-07-31 | 1997-09-15 | Behringwerke Ag | Method for inserting defined sequences at the 3-end of polynucleotides |
US6280935B1 (en) * | 1994-10-13 | 2001-08-28 | Lynx Therapeutics, Inc. | Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags |
US5556752A (en) * | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
US6309843B1 (en) * | 1994-10-25 | 2001-10-30 | The Curators Of The University Of Missouri | Glycoprotein for use in determining endometrial receptivity |
US6194211B1 (en) * | 1994-11-18 | 2001-02-27 | Glaxo Wellcome Inc. | Transcriptional regulatory sequence of carcinoembryonic antigen for expression targeting |
WO1997003210A1 (en) * | 1995-07-11 | 1997-01-30 | FORFÁS (trading as BIORESEARCH IRELAND) | Glycosylase mediated detection of nucleotide sequences at candidate loci |
FR2737223B1 (en) * | 1995-07-24 | 1997-09-12 | Bio Merieux | METHOD OF AMPLIFYING NUCLEIC ACID SEQUENCES BY MOVEMENT USING CHIMERIC PRIMERS |
US6068829A (en) * | 1995-09-11 | 2000-05-30 | The Burnham Institute | Method of identifying molecules that home to a selected organ in vivo |
US6190865B1 (en) * | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
US6197557B1 (en) * | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
EP0991779A1 (en) * | 1997-08-28 | 2000-04-12 | The Perkin-Elmer Corporation | Improved detection of mutations in nucleic acids by chemical cleavage |
US6169194B1 (en) * | 1997-10-16 | 2001-01-02 | Michael Thompson | High surface density covalent immobilization of oligonucleotide monolayers using a 1-(thiotrifluoroacetato)-11-(trichlorososilyl)-undecane linker |
US6090553A (en) * | 1997-10-29 | 2000-07-18 | Beckman Coulter, Inc. | Use of uracil-DNA glycosylase in genetic analysis |
US20010000077A1 (en) * | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
US6174680B1 (en) * | 1998-12-30 | 2001-01-16 | Dana-Farber Cancer Institute, Inc. | Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof |
US6087103A (en) * | 1998-03-04 | 2000-07-11 | Lifespan Biosciences, Inc. | Tagged ligand arrays for identifying target-ligand interactions |
US6225451B1 (en) * | 1998-04-06 | 2001-05-01 | Myriad Genetics, Inc. | Chromosome 11-linked coronary heart disease susceptibility gene CHD1 |
US6440705B1 (en) * | 1998-10-01 | 2002-08-27 | Vincent P. Stanton, Jr. | Method for analyzing polynucleotides |
WO2000039345A1 (en) * | 1998-12-30 | 2000-07-06 | Dana-Farber Cancer Institute, Inc. | Mutation scanning array, and methods of use thereof |
US7074556B2 (en) * | 1999-03-02 | 2006-07-11 | Invitrogen Corporation | cDNA synthesis improvements |
US6339147B1 (en) * | 1999-07-29 | 2002-01-15 | Epoch Biosciences, Inc. | Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids |
EP1218542B1 (en) * | 1999-09-13 | 2004-03-24 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6077674A (en) * | 1999-10-27 | 2000-06-20 | Agilent Technologies Inc. | Method of producing oligonucleotide arrays with features of high purity |
US20010031739A1 (en) * | 1999-12-21 | 2001-10-18 | Dare Akintade Oyedele | Method and kit for quantitating genomic DNA damage and repair capicity |
JP2004513617A (en) * | 2000-06-26 | 2004-05-13 | ニューゲン テクノロジーズ, インコーポレイテッド | Methods and compositions for transcription-based nucleic acid amplification |
US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US6815164B2 (en) * | 2000-10-06 | 2004-11-09 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
CA2430329A1 (en) * | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
EP1390537B1 (en) * | 2001-03-09 | 2013-11-13 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
BR0205268A (en) * | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processes and compositions for mRNA sequence mplification |
US7060441B2 (en) * | 2001-05-04 | 2006-06-13 | Biomerieux | Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling |
EP1275735A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
US20040002371A1 (en) * | 2001-10-01 | 2004-01-01 | Claude Paquin | Method and apparatus for automated system for validating a set of collectible lottery tickets |
US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
US7176025B2 (en) * | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
WO2003078593A2 (en) * | 2002-03-15 | 2003-09-25 | Epigenomics Ag | Discovery and diagnostic methods using 5-methylcytosine dna glycosylase |
EP1490514A4 (en) * | 2002-03-29 | 2008-12-17 | Nugen Technologies Inc | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
US20040137456A1 (en) * | 2002-04-04 | 2004-07-15 | Hiroki Yokota | Method for identifying and characterizing individual dna molecules |
AU2003240795A1 (en) * | 2002-05-24 | 2003-12-12 | Invitrogen Corporation | Nested pcr employing degradable primers |
US7189512B2 (en) * | 2003-02-20 | 2007-03-13 | Noga Porat | Methods for variation detection |
WO2004092418A2 (en) * | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
WO2005003304A2 (en) | 2003-06-20 | 2005-01-13 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
US20050123956A1 (en) * | 2003-09-25 | 2005-06-09 | Affymetrix, Inc. | Methods for modifying DNA for microarray analysis |
US20050136417A1 (en) * | 2003-12-19 | 2005-06-23 | Affymetrix, Inc. | Amplification of nucleic acids |
JP2007524407A (en) * | 2003-12-29 | 2007-08-30 | ニューゲン テクノロジーズ, インコーポレイテッド | Methods for analyzing the methylation status of nucleic acids and methods for fragmentation, labeling and immobilization of nucleic acids |
US20050191682A1 (en) * | 2004-02-17 | 2005-09-01 | Affymetrix, Inc. | Methods for fragmenting DNA |
US7939258B2 (en) * | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
CA2656315A1 (en) | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
WO2009117698A2 (en) * | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
EP2272976A1 (en) | 2009-07-06 | 2011-01-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for differentiation of polynucleotide strands |
US20110224105A1 (en) | 2009-08-12 | 2011-09-15 | Nugen Technologies, Inc. | Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid |
US20110189679A1 (en) | 2009-09-11 | 2011-08-04 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
US20110105364A1 (en) | 2009-11-02 | 2011-05-05 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence selection and amplification |
-
2007
- 2007-07-02 CA CA002656315A patent/CA2656315A1/en not_active Abandoned
- 2007-07-02 US US12/305,633 patent/US20100022403A1/en not_active Abandoned
- 2007-07-02 EP EP07810169.8A patent/EP2038429B1/en not_active Not-in-force
- 2007-07-02 WO PCT/US2007/015409 patent/WO2008005459A2/en active Application Filing
-
2012
- 2012-03-02 US US13/411,170 patent/US8551709B2/en active Active
-
2013
- 2013-08-28 US US14/012,409 patent/US20140065692A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5573913A (en) * | 1994-02-28 | 1996-11-12 | Boehringer Mannheim Gmbh | 3'-RNA labelling with terminal transferase |
US20040005614A1 (en) * | 2002-05-17 | 2004-01-08 | Nurith Kurn | Methods for fragmentation, labeling and immobilization of nucleic acids |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US8334116B2 (en) | 2000-12-13 | 2012-12-18 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7771934B2 (en) | 2000-12-13 | 2010-08-10 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US8071311B2 (en) | 2001-03-09 | 2011-12-06 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US7771946B2 (en) | 2001-03-09 | 2010-08-10 | Nugen Technologies, Inc. | Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences |
US9181582B2 (en) | 2001-03-09 | 2015-11-10 | Nugen Technologies, Inc. | Compositions for amplification of RNA sequences using composite primers |
US8465950B2 (en) | 2003-04-14 | 2013-06-18 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US9175325B2 (en) | 2003-04-14 | 2015-11-03 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8852867B2 (en) | 2005-09-07 | 2014-10-07 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Also Published As
Publication number | Publication date |
---|---|
CA2656315A1 (en) | 2008-01-10 |
EP2038429A2 (en) | 2009-03-25 |
US20140065692A1 (en) | 2014-03-06 |
EP2038429A4 (en) | 2010-03-17 |
EP2038429B1 (en) | 2013-08-21 |
US20120220483A1 (en) | 2012-08-30 |
WO2008005459A2 (en) | 2008-01-10 |
US8551709B2 (en) | 2013-10-08 |
US20100022403A1 (en) | 2010-01-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2008005459A3 (en) | Methods for fragmentation and labeling of nucleic acids | |
WO2007112263A3 (en) | Monomer compositions for the synthesis of rna, methods of synthesis, and methods of deprotection | |
WO2005050171A3 (en) | Methods and compositions relating to single reactive center reagents | |
WO2005065321A3 (en) | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids | |
WO2009135212A3 (en) | Selective 5' ligation tagging of rna | |
BR0010749B1 (en) | improved process for agrobacterium-mediated cotton transformation | |
WO2005012487A3 (en) | Compositions and methods for preparing short rna molecules and other nucleic acids | |
WO2007139723A8 (en) | Chemicallymodified oligonucleotide primers for nucleic acid amplification | |
WO2008046609A3 (en) | Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides | |
WO2008058020A3 (en) | Biological polymeric compositions and methods related thereto | |
WO2008058282A3 (en) | Methods and compositions for large-scale analysis of nucleic acids using dna deletions | |
WO2006120208A8 (en) | Processes for preparing of glucopyranosyl-substituted benzyl-benzene derivatives and intermediates therein | |
WO2008028117A3 (en) | Glp-2 mimetibodies, polypeptides, compositions, methods and uses | |
WO2006044322A3 (en) | Micrornas | |
WO2007142859A3 (en) | Polycrystalline diamond tool for cutting | |
WO2007120624A3 (en) | Concerted nucleic acid assembly reactions | |
WO2007136835A3 (en) | Methods and cells for creating functional diversity and uses thereof | |
WO2005123956A3 (en) | Rapid production of oligonucleotides | |
WO2008058936A3 (en) | Calcium formiate fertiliser | |
WO2005089268A3 (en) | Compositions and methods for optimizing cleavage of rna by rnase h | |
WO2009045067A3 (en) | Primers for pcr amplification comprising abasic parts within the primer sequences | |
WO2008073960A3 (en) | Oligonucleotides containing high concentrations of guanine monomers | |
WO2006074334A3 (en) | Identification of rna targets using helicases | |
WO2006028496A3 (en) | Lesion repair polymerase compositions | |
WO2006046076A3 (en) | Applications of isolated nucleic acid fragments comprising cpg islands |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 07810169 Country of ref document: EP Kind code of ref document: A2 |
|
ENP | Entry into the national phase |
Ref document number: 2656315 Country of ref document: CA |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2007810169 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: RU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12305633 Country of ref document: US |