WO2007056062A3 - Methods for adapting mammalian cells - Google Patents

Methods for adapting mammalian cells Download PDF

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Publication number
WO2007056062A3
WO2007056062A3 PCT/US2006/042815 US2006042815W WO2007056062A3 WO 2007056062 A3 WO2007056062 A3 WO 2007056062A3 US 2006042815 W US2006042815 W US 2006042815W WO 2007056062 A3 WO2007056062 A3 WO 2007056062A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
mammalian cells
adapting
cells
adapting mammalian
Prior art date
Application number
PCT/US2006/042815
Other languages
French (fr)
Other versions
WO2007056062A2 (en
Inventor
Gene W Lee
D Troy Richards
Kathleen Kopycinski
Jill K Fecko
Timothy S Charlebois
Mark Melville
Robin A Heller-Harrison
Martin S Sinacore
Mark Leonard
Original Assignee
Wyeth Corp
Gene W Lee
D Troy Richards
Kathleen Kopycinski
Jill K Fecko
Timothy S Charlebois
Mark Melville
Robin A Heller-Harrison
Martin S Sinacore
Mark Leonard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp, Gene W Lee, D Troy Richards, Kathleen Kopycinski, Jill K Fecko, Timothy S Charlebois, Mark Melville, Robin A Heller-Harrison, Martin S Sinacore, Mark Leonard filed Critical Wyeth Corp
Priority to EP06827375A priority Critical patent/EP1943333A2/en
Priority to JP2008539028A priority patent/JP2009514532A/en
Priority to AU2006312014A priority patent/AU2006312014A1/en
Priority to CA002628340A priority patent/CA2628340A1/en
Priority to BRPI0618178-3A priority patent/BRPI0618178A2/en
Publication of WO2007056062A2 publication Critical patent/WO2007056062A2/en
Publication of WO2007056062A3 publication Critical patent/WO2007056062A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods of adapting cells, e.g., mammalian cells, to a cell culture process are provided. When the adapted cells are genetically modified and used for protein production, they exhibit beneficial characteristics, such as being able to attain higher cell densities and/or achieve a higher overall yield of the produced protein.
PCT/US2006/042815 2005-11-02 2006-11-02 Methods for adapting mammalian cells WO2007056062A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP06827375A EP1943333A2 (en) 2005-11-02 2006-11-02 Methods for adapting mammalian cells
JP2008539028A JP2009514532A (en) 2005-11-02 2006-11-02 Methods for adapting mammalian cells
AU2006312014A AU2006312014A1 (en) 2005-11-02 2006-11-02 Methods for adapting mammalian cells
CA002628340A CA2628340A1 (en) 2005-11-02 2006-11-02 Methods for adapting mammalian cells
BRPI0618178-3A BRPI0618178A2 (en) 2005-11-02 2006-11-02 methods for adapting mammalian cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73281805P 2005-11-02 2005-11-02
US60/732,818 2005-11-02

Publications (2)

Publication Number Publication Date
WO2007056062A2 WO2007056062A2 (en) 2007-05-18
WO2007056062A3 true WO2007056062A3 (en) 2007-08-16

Family

ID=37831644

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/042815 WO2007056062A2 (en) 2005-11-02 2006-11-02 Methods for adapting mammalian cells

Country Status (7)

Country Link
EP (1) EP1943333A2 (en)
JP (1) JP2009514532A (en)
CN (1) CN101300342A (en)
AU (1) AU2006312014A1 (en)
BR (1) BRPI0618178A2 (en)
CA (1) CA2628340A1 (en)
WO (1) WO2007056062A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3255141B1 (en) * 2006-07-13 2021-12-01 Wyeth LLC Production of antibodies with improved glycosylation pattern
PT2356247E (en) 2008-11-12 2015-10-26 Baxalta Inc Method of producing serum-free insulin-free factor vii
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
CN105637085A (en) * 2013-10-11 2016-06-01 瑞泽恩制药公司 Metabolically optimized cell culture
JP7317466B2 (en) * 2017-12-12 2023-07-31 株式会社日立製作所 Screening method for cell lines and culture conditions, and apparatus therefor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020182679A1 (en) * 1997-06-20 2002-12-05 Baxter Healthcare Corporation Recombinant cell clones having increased stability and methods of making and using the same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
JPH03180175A (en) * 1989-12-07 1991-08-06 Snow Brand Milk Prod Co Ltd Serum-free culture medium
EP0953041A4 (en) * 1996-08-30 2003-01-29 Life Technologies Inc Serum-free mammalian cell culture medium, and uses thereof
WO2002016584A2 (en) * 2000-08-23 2002-02-28 Pfizer Products Inc. Process for the preparation of neutrophil inhibitory factor
US6544072B2 (en) * 2001-06-12 2003-04-08 Berg Technologies Electrical connector with metallized polymeric housing
CA2542121A1 (en) * 2003-10-09 2005-04-21 Kyowa Hakko Kogyo Co., Ltd. Genomically modified cell neutralized to serum-free system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020182679A1 (en) * 1997-06-20 2002-12-05 Baxter Healthcare Corporation Recombinant cell clones having increased stability and methods of making and using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SCHUMPP B ET AL: "GROWTH STUDY OF LACTATE AND AMMONIA DOUBLE-RESISTANT CLONES OF HL-60 CELLS", CYTOTECHNOLOGY, vol. 8, no. 1, 1992, pages 39 - 44, XP009081042, ISSN: 0920-9069 *
SINACORE M S ET AL: "CHO DUKX cell lineages preadapted to growth in serum - free suspension culture enable rapid development of cell culture processes for the manufacture of recombinant proteins", BIOTECHNOLOGY AND BIOENGINEERING, INTERSCIENCE PUBLISHERS, LONDON, GB, 20 November 1996 (1996-11-20), XP002077942, ISSN: 0006-3592 *

Also Published As

Publication number Publication date
EP1943333A2 (en) 2008-07-16
JP2009514532A (en) 2009-04-09
CA2628340A1 (en) 2007-05-18
WO2007056062A2 (en) 2007-05-18
AU2006312014A1 (en) 2007-05-18
CN101300342A (en) 2008-11-05
BRPI0618178A2 (en) 2011-08-23

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