WO2007035585A3 - Systems and methods for enrichment of analytes - Google Patents

Systems and methods for enrichment of analytes Download PDF

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Publication number
WO2007035585A3
WO2007035585A3 PCT/US2006/036202 US2006036202W WO2007035585A3 WO 2007035585 A3 WO2007035585 A3 WO 2007035585A3 US 2006036202 W US2006036202 W US 2006036202W WO 2007035585 A3 WO2007035585 A3 WO 2007035585A3
Authority
WO
WIPO (PCT)
Prior art keywords
analytes
methods
enrichment
systems
analyte
Prior art date
Application number
PCT/US2006/036202
Other languages
French (fr)
Other versions
WO2007035585A2 (en
Inventor
Ravi Kapur
Mehmet Toner
Lotien Richard Huang
Tom Barber
Bruce Carvalho
Darren Gray
Ulysses Balis
John Walsh
Michael Grisham
Ron Tompkins
Martin Schmidt
Original Assignee
Living Microsystems Inc
Gen Hospital Corp
Ravi Kapur
Mehmet Toner
Lotien Richard Huang
Tom Barber
Bruce Carvalho
Darren Gray
Ulysses Balis
John Walsh
Michael Grisham
Ron Tompkins
Martin Schmidt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/229,332 external-priority patent/US20070059719A1/en
Priority claimed from US11/228,454 external-priority patent/US20070059716A1/en
Priority claimed from US11/229,037 external-priority patent/US20070059774A1/en
Priority claimed from US11/228,462 external-priority patent/US20070059680A1/en
Priority claimed from US11/229,336 external-priority patent/US20070059781A1/en
Priority claimed from US11/229,328 external-priority patent/US20070059718A1/en
Priority to EP06803736A priority Critical patent/EP1931800A4/en
Priority to AU2006292394A priority patent/AU2006292394A1/en
Application filed by Living Microsystems Inc, Gen Hospital Corp, Ravi Kapur, Mehmet Toner, Lotien Richard Huang, Tom Barber, Bruce Carvalho, Darren Gray, Ulysses Balis, John Walsh, Michael Grisham, Ron Tompkins, Martin Schmidt filed Critical Living Microsystems Inc
Priority to CA002622745A priority patent/CA2622745A1/en
Priority to JP2008531396A priority patent/JP2009509143A/en
Publication of WO2007035585A2 publication Critical patent/WO2007035585A2/en
Publication of WO2007035585A3 publication Critical patent/WO2007035585A3/en
Priority to IL190090A priority patent/IL190090A0/en

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Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/805—Haemoglobins; Myoglobins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/38—Pediatrics
    • G01N2800/385—Congenital anomalies
    • G01N2800/387—Down syndrome; Trisomy 18; Trisomy 13
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Genetics & Genomics (AREA)
  • Theoretical Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention relates to one or more size-based separation modules adapted to increase a concentration of a first analyte in a sample by at least 10,000 fold, wherein said first analyte has an initial concentration in said sample of less than 1 x 10 -3 analytes/µL, and an analyzer for analyzing said first analytes in an enriched medium. Methods of using the separation modules for identifying a characteristic associated with a condition in a patient e.g. a fetal abnormality are also provided.
PCT/US2006/036202 2005-09-15 2006-09-15 Systems and methods for enrichment of analytes WO2007035585A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2008531396A JP2009509143A (en) 2005-09-15 2006-09-15 Analysis improvement system and method
CA002622745A CA2622745A1 (en) 2005-09-15 2006-09-15 Systems and methods for enrichment of analytes
EP06803736A EP1931800A4 (en) 2005-09-15 2006-09-15 Systems and methods for enrichment of analytes
AU2006292394A AU2006292394A1 (en) 2005-09-15 2006-09-15 Systems and methods for enrichment of analytes
IL190090A IL190090A0 (en) 2005-09-15 2008-03-11 Systems and methods for enrichment of analytes

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US11/229,336 2005-09-15
US11/229,332 2005-09-15
US11/229,332 US20070059719A1 (en) 2005-09-15 2005-09-15 Business methods for prenatal Diagnosis
US11/228,462 2005-09-15
US11/229,328 US20070059718A1 (en) 2005-09-15 2005-09-15 Systems and methods for enrichment of analytes
US11/229,336 US20070059781A1 (en) 2005-09-15 2005-09-15 System for size based separation and analysis
US11/228,462 US20070059680A1 (en) 2005-09-15 2005-09-15 System for cell enrichment
US11/229,037 US20070059774A1 (en) 2005-09-15 2005-09-15 Kits for Prenatal Testing
US11/229,037 2005-09-15
US11/228,454 US20070059716A1 (en) 2005-09-15 2005-09-15 Methods for detecting fetal abnormality
US11/228,454 2005-09-15
US11/229,328 2005-09-15

Publications (2)

Publication Number Publication Date
WO2007035585A2 WO2007035585A2 (en) 2007-03-29
WO2007035585A3 true WO2007035585A3 (en) 2007-11-29

Family

ID=37889398

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/036202 WO2007035585A2 (en) 2005-09-15 2006-09-15 Systems and methods for enrichment of analytes

Country Status (6)

Country Link
EP (1) EP1931800A4 (en)
JP (1) JP2009509143A (en)
AU (1) AU2006292394A1 (en)
CA (1) CA2622745A1 (en)
IL (1) IL190090A0 (en)
WO (1) WO2007035585A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US9212977B2 (en) 2005-01-18 2015-12-15 Biocept, Inc. Cell separation using microchannel having patterned posts

Families Citing this family (25)

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WO2006078470A2 (en) 2005-01-18 2006-07-27 Biocept, Inc. Cell separation using microchannel having patterned posts
US8158410B2 (en) 2005-01-18 2012-04-17 Biocept, Inc. Recovery of rare cells using a microchannel apparatus with patterned posts
GB2427468B (en) * 2005-04-05 2011-03-02 Cellpoint Diagnostics Cell separation device and method for the detection of EpCAM positive cells
US7695956B2 (en) 2006-01-12 2010-04-13 Biocept, Inc. Device for cell separation and analysis and method of using
US20080026390A1 (en) * 2006-06-14 2008-01-31 Roland Stoughton Diagnosis of Fetal Abnormalities by Comparative Genomic Hybridization Analysis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
FR2931141B1 (en) * 2008-05-13 2011-07-01 Commissariat Energie Atomique MICROFLUIDIC SYSTEM AND METHOD FOR THE SORTING OF AMAS FROM CELLS AND PREFERENCE FOR CONTINUOUS ENCAPSULATION THROUGH THEIR SORTING
US20110212440A1 (en) * 2008-10-10 2011-09-01 Cnrs-Dae Cell sorting device
WO2010122158A1 (en) * 2009-04-23 2010-10-28 Dublin City University A lateral flow assay device for coagulation monitoring and method thereof
SG184592A1 (en) * 2011-03-18 2012-10-30 Univ Singapore Isolating target cells from a biological fluid
WO2012008839A2 (en) 2010-07-16 2012-01-19 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patiëntenzorg A method of analysing a blood sample of a subject for the presence of a disease marker
JP5626727B2 (en) * 2010-09-21 2014-11-19 国立大学法人東京農工大学 Analysis method of leukocyte population from micro blood
AU2012231884B2 (en) 2011-03-18 2017-03-09 Stichting Vumc A method of analysing a blood sample of a subject for the presence of a disease marker
CN105247042B (en) 2013-03-15 2021-06-11 普林斯顿大学理事会 Method and apparatus for high throughput purification
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
CN110186835B (en) 2013-03-15 2022-05-31 Gpb科学有限公司 On-chip microfluidic processing of particles
JP6403190B2 (en) * 2013-08-06 2018-10-10 旭化成株式会社 Microchannel structure and particle separation method
ES1111981Y (en) 2014-05-22 2014-09-03 Ayet Félix Checa SURGICAL PROTESIS FOR THE TREATMENT OF INGUINAL HERNIAS
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
EP3754011B1 (en) 2015-09-09 2022-02-16 Drawbridge Health, Inc. Devices for sample collection, stabilization and preservation
CN115251923A (en) 2017-01-10 2022-11-01 集联健康有限公司 Devices, systems, and methods for sample collection
CA3074495A1 (en) 2017-09-01 2019-03-07 Gpb Scientific, Llc Methods for preparing therapeutically active cells using microfluidics
JPWO2020184500A1 (en) * 2019-03-14 2020-09-17
CN116018522A (en) * 2020-09-29 2023-04-25 Nok株式会社 White blood cell capturing device
CN116312809B (en) * 2023-04-10 2023-08-29 杭州美赛生物医药科技有限公司 Cell screening method based on antibody coupled magnetic beads

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US20040048360A1 (en) * 1999-08-26 2004-03-11 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
WO2004029221A2 (en) * 2002-09-27 2004-04-08 The General Hospital Corporation Microfluidic device for cell separation and uses thereof

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US6074827A (en) * 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
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US7150812B2 (en) * 2002-10-23 2006-12-19 The Trustees Of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
US20040048360A1 (en) * 1999-08-26 2004-03-11 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
WO2004029221A2 (en) * 2002-09-27 2004-04-08 The General Hospital Corporation Microfluidic device for cell separation and uses thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9212977B2 (en) 2005-01-18 2015-12-15 Biocept, Inc. Cell separation using microchannel having patterned posts
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags

Also Published As

Publication number Publication date
EP1931800A2 (en) 2008-06-18
IL190090A0 (en) 2008-08-07
WO2007035585A2 (en) 2007-03-29
JP2009509143A (en) 2009-03-05
EP1931800A4 (en) 2011-06-15
CA2622745A1 (en) 2007-03-29
AU2006292394A1 (en) 2007-03-29

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