WO2007034169A3 - Cell selection and reporter system - Google Patents
Cell selection and reporter system Download PDFInfo
- Publication number
- WO2007034169A3 WO2007034169A3 PCT/GB2006/003484 GB2006003484W WO2007034169A3 WO 2007034169 A3 WO2007034169 A3 WO 2007034169A3 GB 2006003484 W GB2006003484 W GB 2006003484W WO 2007034169 A3 WO2007034169 A3 WO 2007034169A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- fusion
- enzyme
- cell selection
- sorting
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A fusion protein has three domains, being an extracellular cell surface antigen or a fluorescent protein for sorting uses, an enzyme that enables staining of cells in tissues or tissue sections and an enzyme, such as antibiotic resistance, which protects cells from the effects of a toxic factor in culture medium. The fusion is used for purification and sorting of cells expressing the fusion under control of a cell or tissue-specific promoter and subsequent tracking and analyses of cells post transplantation.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0519197A GB0519197D0 (en) | 2005-09-20 | 2005-09-20 | Cell selection and reporter system |
GB0519197.8 | 2005-09-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007034169A2 WO2007034169A2 (en) | 2007-03-29 |
WO2007034169A3 true WO2007034169A3 (en) | 2007-05-31 |
Family
ID=35249114
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2006/003484 WO2007034169A2 (en) | 2005-09-20 | 2006-09-20 | Cell selection and reporter system |
Country Status (3)
Country | Link |
---|---|
GB (1) | GB0519197D0 (en) |
TW (1) | TW200804598A (en) |
WO (1) | WO2007034169A2 (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0731169A1 (en) * | 1995-01-10 | 1996-09-11 | University Of Edinburgh | Vectors and use thereof for capturing target genes |
WO2003014361A1 (en) * | 2001-08-02 | 2003-02-20 | Altana Pharma Ag | Novel recombinant gene expression method by stop codon suppression |
-
2005
- 2005-09-20 GB GB0519197A patent/GB0519197D0/en not_active Ceased
-
2006
- 2006-09-20 WO PCT/GB2006/003484 patent/WO2007034169A2/en active Application Filing
- 2006-09-20 TW TW95134772A patent/TW200804598A/en unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0731169A1 (en) * | 1995-01-10 | 1996-09-11 | University Of Edinburgh | Vectors and use thereof for capturing target genes |
WO2003014361A1 (en) * | 2001-08-02 | 2003-02-20 | Altana Pharma Ag | Novel recombinant gene expression method by stop codon suppression |
Also Published As
Publication number | Publication date |
---|---|
WO2007034169A2 (en) | 2007-03-29 |
GB0519197D0 (en) | 2005-10-26 |
TW200804598A (en) | 2008-01-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2007028106A3 (en) | Host cell lines for production of antibody constant region with enhanced effector function | |
Krupinska et al. | New insights into plastid nucleoid structure and functionality | |
DE602005016218D1 (en) | CONTROLLED READING OF STOP CODONS | |
WO2005100395A3 (en) | EXPRESSION SYSTEM FOR PREPARING IL-15/Fc FUSION PROTEINS AND ITS USE | |
WO2007095350A3 (en) | Compositions and methods for use of pigment epithelial derived factor (pedf) peptide fragments | |
WO2006127809A3 (en) | Methods for expansion and analysis of cultured hematopoietic stem cells | |
WO2006050050A3 (en) | Chemically defined media compositions | |
WO2002077210A3 (en) | Methods to culture circovirus | |
WO2006124641A3 (en) | Anti-mesothelin antibodies useful for immunological assays | |
WO2006116181A3 (en) | Regulatory t cell mediator proteins and uses thereof | |
WO2007075873A3 (en) | Protein engineering strategies to optimize activity of surface attached proteins | |
WO2006069403A3 (en) | Methods for producing soluble multi-membrane-spanning proteins | |
WO2006062877A3 (en) | Protein subcellular localization assays using split fluorescent proteins | |
WO1999061619A3 (en) | Cell cycle genes, proteins and uses thereof | |
GB2429211A (en) | Feeder independent extended culture of embryonic stem cells | |
EP3275901A8 (en) | Human embryonic stem cell methods and podxl expression | |
WO2007044418A3 (en) | Cell culture media, kits and methods of use | |
EP2641911A3 (en) | Compositions and methods for re-programming cells without genetic modification | |
WO2008121324A3 (en) | Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells | |
WO2007055823A3 (en) | Gpcr expressing cell lines and antibodies | |
EP1637607A3 (en) | Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof | |
WO2006107813A3 (en) | Site specific incorporation of phosphoserine into polypeptides using phosphoseryl-trna synthetase | |
WO2004042003A3 (en) | Cell lysis compositions, methods of use, apparatus, and kit | |
WO2009083790A3 (en) | 'apoptosis inducing positive control for expression modulating experiments' | |
Schulz | Long-distance trafficking: lost in transit or stopped at the gate? |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DPE1 | Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101) | ||
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 06779489 Country of ref document: EP Kind code of ref document: A2 |