WO2007034169A3 - Cell selection and reporter system - Google Patents

Cell selection and reporter system Download PDF

Info

Publication number
WO2007034169A3
WO2007034169A3 PCT/GB2006/003484 GB2006003484W WO2007034169A3 WO 2007034169 A3 WO2007034169 A3 WO 2007034169A3 GB 2006003484 W GB2006003484 W GB 2006003484W WO 2007034169 A3 WO2007034169 A3 WO 2007034169A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
fusion
enzyme
cell selection
sorting
Prior art date
Application number
PCT/GB2006/003484
Other languages
French (fr)
Other versions
WO2007034169A2 (en
Inventor
Timothy Allsopp
Lillian Alexandra Hook
Original Assignee
Stem Cell Sciences Uk Ltd
Timothy Allsopp
Lillian Alexandra Hook
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stem Cell Sciences Uk Ltd, Timothy Allsopp, Lillian Alexandra Hook filed Critical Stem Cell Sciences Uk Ltd
Publication of WO2007034169A2 publication Critical patent/WO2007034169A2/en
Publication of WO2007034169A3 publication Critical patent/WO2007034169A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A fusion protein has three domains, being an extracellular cell surface antigen or a fluorescent protein for sorting uses, an enzyme that enables staining of cells in tissues or tissue sections and an enzyme, such as antibiotic resistance, which protects cells from the effects of a toxic factor in culture medium. The fusion is used for purification and sorting of cells expressing the fusion under control of a cell or tissue-specific promoter and subsequent tracking and analyses of cells post transplantation.
PCT/GB2006/003484 2005-09-20 2006-09-20 Cell selection and reporter system WO2007034169A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0519197A GB0519197D0 (en) 2005-09-20 2005-09-20 Cell selection and reporter system
GB0519197.8 2005-09-20

Publications (2)

Publication Number Publication Date
WO2007034169A2 WO2007034169A2 (en) 2007-03-29
WO2007034169A3 true WO2007034169A3 (en) 2007-05-31

Family

ID=35249114

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/003484 WO2007034169A2 (en) 2005-09-20 2006-09-20 Cell selection and reporter system

Country Status (3)

Country Link
GB (1) GB0519197D0 (en)
TW (1) TW200804598A (en)
WO (1) WO2007034169A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0731169A1 (en) * 1995-01-10 1996-09-11 University Of Edinburgh Vectors and use thereof for capturing target genes
WO2003014361A1 (en) * 2001-08-02 2003-02-20 Altana Pharma Ag Novel recombinant gene expression method by stop codon suppression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0731169A1 (en) * 1995-01-10 1996-09-11 University Of Edinburgh Vectors and use thereof for capturing target genes
WO2003014361A1 (en) * 2001-08-02 2003-02-20 Altana Pharma Ag Novel recombinant gene expression method by stop codon suppression

Also Published As

Publication number Publication date
WO2007034169A2 (en) 2007-03-29
GB0519197D0 (en) 2005-10-26
TW200804598A (en) 2008-01-16

Similar Documents

Publication Publication Date Title
WO2007028106A3 (en) Host cell lines for production of antibody constant region with enhanced effector function
Krupinska et al. New insights into plastid nucleoid structure and functionality
DE602005016218D1 (en) CONTROLLED READING OF STOP CODONS
WO2005100395A3 (en) EXPRESSION SYSTEM FOR PREPARING IL-15/Fc FUSION PROTEINS AND ITS USE
WO2007095350A3 (en) Compositions and methods for use of pigment epithelial derived factor (pedf) peptide fragments
WO2006127809A3 (en) Methods for expansion and analysis of cultured hematopoietic stem cells
WO2006050050A3 (en) Chemically defined media compositions
WO2002077210A3 (en) Methods to culture circovirus
WO2006124641A3 (en) Anti-mesothelin antibodies useful for immunological assays
WO2006116181A3 (en) Regulatory t cell mediator proteins and uses thereof
WO2007075873A3 (en) Protein engineering strategies to optimize activity of surface attached proteins
WO2006069403A3 (en) Methods for producing soluble multi-membrane-spanning proteins
WO2006062877A3 (en) Protein subcellular localization assays using split fluorescent proteins
WO1999061619A3 (en) Cell cycle genes, proteins and uses thereof
GB2429211A (en) Feeder independent extended culture of embryonic stem cells
EP3275901A8 (en) Human embryonic stem cell methods and podxl expression
WO2007044418A3 (en) Cell culture media, kits and methods of use
EP2641911A3 (en) Compositions and methods for re-programming cells without genetic modification
WO2008121324A3 (en) Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells
WO2007055823A3 (en) Gpcr expressing cell lines and antibodies
EP1637607A3 (en) Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof
WO2006107813A3 (en) Site specific incorporation of phosphoserine into polypeptides using phosphoseryl-trna synthetase
WO2004042003A3 (en) Cell lysis compositions, methods of use, apparatus, and kit
WO2009083790A3 (en) 'apoptosis inducing positive control for expression modulating experiments'
Schulz Long-distance trafficking: lost in transit or stopped at the gate?

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 06779489

Country of ref document: EP

Kind code of ref document: A2