WO2007006180A1 - A system for analyzing trace quantity of organic substances in urine and its application - Google Patents
A system for analyzing trace quantity of organic substances in urine and its application Download PDFInfo
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- WO2007006180A1 WO2007006180A1 PCT/CN2005/001044 CN2005001044W WO2007006180A1 WO 2007006180 A1 WO2007006180 A1 WO 2007006180A1 CN 2005001044 W CN2005001044 W CN 2005001044W WO 2007006180 A1 WO2007006180 A1 WO 2007006180A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/493—Physical analysis of biological material of liquid biological material urine
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- the present invention relates to a system for analyzing trace organic substances in biological urine, and more particularly to a method for measuring the type and concentration of organic compounds in urine using a polarography technique using solid electrodes.
- the disclosed method can provide physiological information of the organism for further analysis and judgment purposes. Background technique
- the metabolic reaction of the organism is manifested in changes in the type and content of trace organic substances in the urine of the organism.
- humans such as pregnancy or not, drug use, steroid use, AIDS infection, etc.
- urine detection techniques and products not just humans and animals, such as veterinarians or livestock, are applied to urine testing techniques. Therefore, it is known that the detection of trace organic substances in urine is an important technique for diagnosing the physiological state of living organisms.
- the main difficulty lies in how to detect certain traces of specific organic substances in complex urine samples.
- Polarographs can analyze not only the elements of the periodic table, but also most of the organic matter. For example, monosaccharides, open rather than cyclic ketones or guanidines, some pure amino acids, such as: cysteine (CYSTEINES histidine (HISTIDINE), aspartic acid (ASPARTIC ACID), arginine (ARGININE) , asparagine (ASPARAGINE), methionine (METHIONINE), etc.
- This method can be qualitative and quantitative at the same time, and different substances are measured in the same experiment, with high sensitivity and accuracy.
- Faster features, and the results are more objective, can automatically obtain information, can also measure the trace solution and biological body fluids, and the results of these tests can provide information to the experimenter to the physiological state of the tested organism Judge.
- the configuration of a conventional polar polarography is filled with a solution to be tested in an electrolytic cell 03, in which a mercury drop electrode 01 and a calomel electrode 02 are immersed.
- the calomel electrode 02 is an anode (reference electrode)
- the mercury droplet electrode 01 is a cathode (working electrode).
- the mercury drop electrode is composed of a mercury storage tank 011 connected to the capillary 013 through a rubber tube 012. The speed at which the mercury droplets fall can be adjusted by adjusting the height of the mercury storage tank, typically 2 to 3 drops per 10 seconds. '
- mercury has many disadvantages, such as high risk, especially for experimental or clinical use. On, it is not suitable. Mercury causes many acute and chronic diseases. Therefore, the location of mercury must be isolated and special equipment must be provided. Otherwise, it will cause danger, and mercury vapor will cause big problems in non-ventilated workplaces. In the industry, when the voltage exceeds 0.3 volts, it can not be used, because the dissolution of mercury will occur at this time.
- the conventional polarograph still has other shortcomings, that is, in the analysis of the results, the tester needs to read the value or pointer displayed by the ammeter or the voltmeter as a judgment.
- the disadvantages are: First, the lack of precision, using the current meter, the pointer or waveform displayed by the voltmeter to identify, this is still basically a "visual" method, the smaller waveform and pointer changes will not be able to The subject is identified. Second, because the observation accuracy of the waveform is related to the experience of the observer, it takes a long time to train the operator. Third, different detectors may have slightly different interpretations of the same waveform, and the results of the detection cannot be objective.
- the object of the present invention is to provide a detection system for analyzing trace organic substances in urine, which is a system using solid-state electrode polarography or a combination of the solid-state electrode polarograph and computer information technology.
- a detection system for analyzing trace organic substances in urine which is a system using solid-state electrode polarography or a combination of the solid-state electrode polarograph and computer information technology.
- systems combining solid-state electrode polarography and computer information technology can automatically analyze the results of the test by comparing "measured polarographic maps" with "judged standard data”.
- Another object of the present invention is to provide a detection system for analyzing the concentration of trace organic substances in urine, which has at least one connecting end by using a polarograph; a graphite electrode connected to a connecting end of the polarimeter through a wire. As a working electrode; and a metal electrode connected to a connection end of the polarimeter by a wire as a reference electrode, the system can detect trace organic substances in urine such as: monosaccharide, open rather than cyclic ketone or Terpenoids, concentrations of some pure amino acids, etc.
- the detection system of the solid-state electrode polarograph which uses the graphite electrode processed by the degassing filling process as the working electrode, and must use the following steps when using the detection system: 1. Collecting urine of a living organism as a carcass, which may be a human or a domestic animal;
- the tester knows the type and concentration of trace organic matter in the urine of the test organism from the voltage-current waveform on the polarogram;
- the physiological state of the organism is judged by the "type and concentration of trace organic substances in the urine".
- the waveform of the polarogram is nothing more than a voltage-current signal that changes with time, it can be read, memorized and stored in a computer system by electronic information technology. Therefore, the pole of the solid-state electrode
- the spectrometer is connected to the computer system, and transmits the waveform value of the polarogram to the computer system through a communication interface such as RS232, USB, etc., and stores it in the memory, and then uses the comparison module to the pole
- the spectrum is compared with "judgment standard data", which is a number or figure from which the physiological condition corresponding to the polarogram can be judged. In this way, electronic information technology can be used to automatically analyze the test results to assist or replace the original judgment method.
- FIG. 2 is a schematic diagram of a system for analyzing trace organic substances in urine for use in the present invention.
- 3 is a system for automatically analyzing a trace amount of organic matter in urine according to the present invention.
- Fig. 4 is a comparative flow chart of a system for automatically analyzing trace organic substances in urine according to the present invention.
- the main part represents the mark description
- Electrolytic cell 04 Voltmeter 05: Ammeter 06: Polarograph
- Step 110 Steps to collect urine samples
- Step 120 Step of placing a urine sample into the reference electrode and the working electrode of the polarograph to generate a voltage-current signal
- Step 130 Steps of transmitting a voltage-current signal to a computer device to generate a polarogram a
- Step 140 Steps of recording the polarogram ⁇ into the memory device
- Step. 15Q Steps to analyze the physiological state of the polarogram ⁇ by comparing the modules and judging the criteria
- Step 160 Step of displaying the compared result on the display
- the figure is a schematic diagram of a system for analyzing trace organic matter in urine. It includes a polarograph 06 (integrating the original voltage, galvanometer, voltmeter, etc. components of the polarograph in Figure 1 into a single case), the anode of which is connected to the metal electrode 07 through a wire (Reference) Electrode), the cathode of the polarograph 06 is connected to a graphite electrode 08 (working electrode).
- the system can be used to detect trace amounts of organic substances in urine such as monosaccharides, open rather than cyclic ketones or acids, and some pure amino acids such as: cysteine (CYSTEINE), histidine (HISTIDINE), aspartame Acid (ASPARTIC ACID).
- the polarograph 06 can further comprise a connection output device such as: a general printer or display Wait.
- Degassing process removing the air molecules in the graphite electrode structure by using a vacuum system
- the filler is filled into the gap of the graphite electrode structure.
- the purpose of this "degassing filling process” is to remove the air in the voids in the graphite electrode structure to reduce the molecules that interfere with the electrolytic reaction, and the other purpose is to remove the air molecules present in the voids of the graphite electrode. It is also advantageous for the filler to fill the voids in the graphite electrode structure so that the air does not enter the voids again.
- FIG 3 is a system for automatically analyzing trace organic matter in urine.
- the polarograph 10 is connected to a processed graphite electrode (working electrode) 08.
- the graphite electricity The main material of the pole 08 is graphite, and the others may be glass graphite, stone carbon paste, and spectral carbon.
- the outer edge of the graphite electrode 08 is coated with a layer of filler.
- the filler is paraffin, and other equivalents such as wax, glue, rosin, and the like.
- the material of the metal electrode (reference electrode) 07, aluminum is used in this embodiment, and other equivalents such as: gold, platinum, silver, titanium, molybdenum, vanadium, tungsten, hard aluminum.
- the metal electrode 07 is formed into a cup shape in which a urine sample 31 is placed.
- the graphite electrode 08 is immersed in the urine sample 31 (but not in contact with the metal electrode 07).
- the polarimeter 10 is connected to the information analysis device 40.
- the information analysis device 40 includes a waveform access module 50.
- the waveform access module 50 can read the polarographic device 10 over time.
- the voltage-current value i.e., the polarogram
- the memory 60 is stored in the memory 60.
- the comparison module 70 is connected to the memory 60, and can read the polarogram stored in the memory 60 and compare it with the number or the graphic in the judgment standard module 80 to obtain the comparison result 90, and output the comparison result.
- the display 100 can be a computer screen, a printer, or a wired, wireless network system, so that the results can be output to the other end of the distance.
- the waveform access module 50 reads the polarogram measured by the polarimeter 10 every 0.001 seconds, and reads a total of 5 seconds, for a total of 5000. Voltage-current value.
- the 5000 data which is the polarographic waveform data of the urine sample 31, is simply referred to as the polarogram ⁇ for the convenience of description.
- the comparison module 70 has a specific comparison method.
- the program is:
Abstract
A system for analyzing trace quantity of organic substances in urine comprises a polarograph, a graphite electrode, a metallic electrode and an information-analyzing device. The information-analyzing device comprises a memorizer stored with a judgment criterion module, a control module and a display. The system is used for urine detecting. It stores the polarograph created by the polarograph into the memorizer, compares with the normal module through the control module, obtains the component and quantity of organic substances in urine, and displays the result.
Description
技术领域 Technical field
本发明涉及一种分析生物尿液中微量有机物质的***，特别是涉 及一种有关于使用固态电极的极谱仪 (Polarography)技术，对尿液中 的有机化合物进行种类和浓度的测量，本发明所揭示的方法可以提供 生物体的生理上的信息， 以供作进一步的分析与判断用途。 背景技术 The present invention relates to a system for analyzing trace organic substances in biological urine, and more particularly to a method for measuring the type and concentration of organic compounds in urine using a polarography technique using solid electrodes. The disclosed method can provide physiological information of the organism for further analysis and judgment purposes. Background technique
生物体的代谢反应， 不论是饮食、 生病、用药、脏器的生理病变 等等，都会表现于生物体尿液中微量有机物质的种类和含量的变化上 面。 以人类为例， 例如怀孕与否、 是否有吸毒、 类固醇的服用、 爱滋 病的感染等等，都有相关的尿液检测技术和产品。而不单单是人类和 动物， 如兽医或是畜牧， 都会应用到尿液检测的技术。 因此可知， 尿 液中微量有机物质的检测， 为一种诊断生物体生理况状上重要的技 术。 在尿液检测技术的开发上，主要的困难点在于如何在成分复杂的 尿液检体中， 检测某几种微量的、特定的有机物质。在各种检测技术 中， 电化分析中的极谱仪 (Polarography)检测法， 为一种在特殊条件 下，对待测物质的溶液进行电解，并对所得到的电流一电压曲线进行 分析的方法。 其理论基础在于对待测物质的电解物质， 进行电观测，
并依照对电的依赖性，判断该受测物的性质和数量。极谱仪包含有两 个电极： 参考电极和工作电极。 测试时， 需将受试物置于电解液中， 接上该电极进行电解后， 以收集电解质作为判断的依据。 The metabolic reaction of the organism, whether it is diet, illness, medication, physiological lesions of organs, etc., is manifested in changes in the type and content of trace organic substances in the urine of the organism. For example, humans, such as pregnancy or not, drug use, steroid use, AIDS infection, etc., have related urine detection techniques and products. Not just humans and animals, such as veterinarians or livestock, are applied to urine testing techniques. Therefore, it is known that the detection of trace organic substances in urine is an important technique for diagnosing the physiological state of living organisms. In the development of urine detection technology, the main difficulty lies in how to detect certain traces of specific organic substances in complex urine samples. Among various detection techniques, the Polarography method in electrochemical analysis is a method of electrolyzing a solution of a substance to be tested under special conditions and analyzing the obtained current-voltage curve. The theoretical basis is based on the electrolytic substance of the substance to be measured, and electrical observation is performed. And according to the dependence on electricity, determine the nature and quantity of the tested object. The polarograph contains two electrodes: a reference electrode and a working electrode. During the test, the test object should be placed in the electrolyte, and after the electrode is connected for electrolysis, the electrolyte is collected as the basis for judgment.
极谱仪不仅可以分析周期表中的元素，也可以分析大部分的有机 物质。例如，单醣、开放而非环状的酮或酴类、一些纯氨基酸，例如： 半胱氨酸（CYSTEINES组氨酸（HISTIDINE)、天门冬氨酸（ASPARTIC ACID ) , 精氨酸 （ARGININE )、 天冬酰胺 （ ASPARAGINE )、 蛋氨酸 (METHIONINE)等等。此方法同时可以定性和定量， 并在同一实验中 测出不同的物质， 具有高度的感度和精确性。和其它方法相比较， 则 具有较快速的特点， 且结果较为客观， 可自动获得信息， 也可测微量 溶液和生物的体液，而这一些检测的结果，便可以提供信息给实验者 来对受检测的生物体的生理状况进行判断。 Polarographs can analyze not only the elements of the periodic table, but also most of the organic matter. For example, monosaccharides, open rather than cyclic ketones or guanidines, some pure amino acids, such as: cysteine (CYSTEINES histidine (HISTIDINE), aspartic acid (ASPARTIC ACID), arginine (ARGININE) , asparagine (ASPARAGINE), methionine (METHIONINE), etc. This method can be qualitative and quantitative at the same time, and different substances are measured in the same experiment, with high sensitivity and accuracy. Compared with other methods, Faster features, and the results are more objective, can automatically obtain information, can also measure the trace solution and biological body fluids, and the results of these tests can provide information to the experimenter to the physiological state of the tested organism Judge.
传统式极谱仪（classical polarography)的构造， 如图 1所示， 在电解池 03中装有待测溶液， 其中浸入滴汞电极 01和甘汞电极 02。 此时， 甘汞电极 02为阳极 (参考电极）， 滴汞电极 01则为阴极 (工作 电极)。 滴汞电极是由蓄汞槽 011通过橡皮管 012与毛细管 013相连 接而组成的。通过调节蓄汞槽的高度可调节汞滴下落的速度，一般为 每 10秒 2〜3滴。 ' The configuration of a conventional polar polarography, as shown in Fig. 1, is filled with a solution to be tested in an electrolytic cell 03, in which a mercury drop electrode 01 and a calomel electrode 02 are immersed. At this time, the calomel electrode 02 is an anode (reference electrode), and the mercury droplet electrode 01 is a cathode (working electrode). The mercury drop electrode is composed of a mercury storage tank 011 connected to the capillary 013 through a rubber tube 012. The speed at which the mercury droplets fall can be adjusted by adjusting the height of the mercury storage tank, typically 2 to 3 drops per 10 seconds. '
电解时， 加一电位差于该两个电极上， 并以电压计 04指示其数 值， 电流计 05记录相应的电流值。 然而， 汞有很多的缺点， 如危险性高， 特别是使用于实验或临床
上， 极不适用。汞造成很多急慢性疾病， 因此汞的工乍地点， 必需隔 离， 也必需有特别的设备， 否则会产生危险， 且汞的蒸气在不通风的 工作场所， 也将造成大问题。在工业上， 当电压超过 0. 3伏特时也无 法使用， 因为此时将会发生汞阳离性的溶解， 当电解速度增加时， 汞 从毛细管中流出，示波器上所显示的半波电位在示波器的进程中，半 波电位产生， 并且在时间轴的变换中， 电脉冲已经达到另一滴汞， 汞 液滴的伸展常常不允许在需要的电场进行分析，也无法从示波器中显 示出来。在其它同样的条件之下， 汞液滴的间隔， 将随着所失电位的 大小而定， 如此一来， 在副电压扩大得大于电微管极大值， 极谱仪就 产生扩散电流的下降，装置电流常是阻碍的因素，汞液滴无法跟得上 电极的反应， 换言之， 检测的速度是受限的。 另外， 在高温时汞很快 的蒸发，蒸发的汞也会带走部分的待测物质，这对于微量物质的捡测 上， 会造成检测结果不精确的问题。 During electrolysis, a potential difference is applied to the two electrodes, and the value is indicated by a voltmeter 04, and the current meter 05 records the corresponding current value. However, mercury has many disadvantages, such as high risk, especially for experimental or clinical use. On, it is not suitable. Mercury causes many acute and chronic diseases. Therefore, the location of mercury must be isolated and special equipment must be provided. Otherwise, it will cause danger, and mercury vapor will cause big problems in non-ventilated workplaces. In the industry, when the voltage exceeds 0.3 volts, it can not be used, because the dissolution of mercury will occur at this time. When the electrolysis speed increases, mercury flows out of the capillary, and the half-wave potential shown on the oscilloscope is In the course of the oscilloscope, the half-wave potential is generated, and in the time-axis transformation, the electric pulse has reached another drop of mercury. The stretching of the mercury droplets is often not allowed to be analyzed at the required electric field, nor can it be displayed from the oscilloscope. Under other similar conditions, the interval of mercury droplets will vary with the magnitude of the potential lost. As a result, when the sub-voltage is amplified larger than the maximum value of the micro-tube, the polarograph generates a diffusion current. Falling, the device current is often a hindrance factor, and the mercury droplets cannot keep up with the reaction of the electrodes. In other words, the speed of detection is limited. In addition, mercury evaporates quickly at high temperatures, and the evaporated mercury also carries away some of the substance to be tested. This may cause inaccurate detection results for the detection of trace substances.
除了因使用汞电极而产生的问题， 传统式极谱仪仍有其它缺点， 那就是在结果分析上，检测者需读取电流计、电压计所显示的数值或 指针作为判断。 此缺点在于： 第一， 精确度的不足， 以电流计、 电压 计所显示的指针或波形来进行辨别， 这基本上仍是一种 "目测"的方 法， 较小的波形与指针变化将无法被检测者所辨别。第二， 因为波形 的观察准确度，与观察者的经验有关，所以需要较长的时间来培训操 作人员。第三， 不同的检测者对相同的波形的解释， 可能会出现些微 的差异， 检测的结果无法客观化。 In addition to the problems caused by the use of mercury electrodes, the conventional polarograph still has other shortcomings, that is, in the analysis of the results, the tester needs to read the value or pointer displayed by the ammeter or the voltmeter as a judgment. The disadvantages are: First, the lack of precision, using the current meter, the pointer or waveform displayed by the voltmeter to identify, this is still basically a "visual" method, the smaller waveform and pointer changes will not be able to The subject is identified. Second, because the observation accuracy of the waveform is related to the experience of the observer, it takes a long time to train the operator. Third, different detectors may have slightly different interpretations of the same waveform, and the results of the detection cannot be objective.
总合上述， 我们可知在传统技术中， 采用汞作为电极的极谱仪，
在大量临床应用上所具有的一些问题， 还有显示检测结果的显示器， 例如示波器， 在检测结果的判断和分析上， 仍存在有不够精确， 以及 需要较长时间来培训操作人员的问题，尚未可称为可大量应用于临床 上。 因此， 如何解决汞电极的问题与提升 "分析检测结果"的能力， 便是我们所需面对与克服的问题。 发明内容 In summary, we know that in the traditional technology, mercury is used as the polarograph of the electrode. Some problems in a large number of clinical applications, as well as displays that display test results, such as oscilloscopes, there are still inaccuracies in the judgment and analysis of test results, and it takes a long time to train operators. It can be said to be widely used in clinical practice. Therefore, how to solve the problem of mercury electrodes and improve the ability to "analyze test results" is what we need to face and overcome. Summary of the invention
本发明的目的，在于提出一种分析尿液中微量有机物质的检测系 统，该检测***为一种使用固态电极极谱仪 (polarography) ,或结合 该固态电极极谱仪和计算机信息科技的***，特别是结合固态电极极 谱仪和计算机信息科技的***， 能够通过对比 "所测得的极谱图"和 "判断标准数据"， 自动的分析出检测的结果。 The object of the present invention is to provide a detection system for analyzing trace organic substances in urine, which is a system using solid-state electrode polarography or a combination of the solid-state electrode polarograph and computer information technology. In particular, systems combining solid-state electrode polarography and computer information technology can automatically analyze the results of the test by comparing "measured polarographic maps" with "judged standard data".
本发明的另一目的，在于提出一种分析尿液中微量有机物质浓度 的检测***， 利用极谱仪， 具有至少一个连接端； 石墨电极， 透过导 线连接于该极谱仪的一连接端， 作为工作电极； 及金属电极， 通过导 线连接于该极谱仪的一连接端，作为参考电极，利用本***可以检测 尿液中微量有机物质如： 单醣、开放而非环状的酮或酴类、一些纯氨 基酸的浓度等。 Another object of the present invention is to provide a detection system for analyzing the concentration of trace organic substances in urine, which has at least one connecting end by using a polarograph; a graphite electrode connected to a connecting end of the polarimeter through a wire. As a working electrode; and a metal electrode connected to a connection end of the polarimeter by a wire as a reference electrode, the system can detect trace organic substances in urine such as: monosaccharide, open rather than cyclic ketone or Terpenoids, concentrations of some pure amino acids, etc.
固态电极极谱仪的检测***，该极谱仪使用除气填充工艺加工的 石墨电极作为工作电极，在使用该检测***时必需要经过下面几个步 骤：
1.收集生物的尿液作为捡体， 该生物体可以是人类或 是家畜； The detection system of the solid-state electrode polarograph, which uses the graphite electrode processed by the degassing filling process as the working electrode, and must use the following steps when using the detection system: 1. Collecting urine of a living organism as a carcass, which may be a human or a domestic animal;
2.利用该固态电极极谱来检测该尿液，并得到极谱图； 2. Using the solid electrode polarography to detect the urine and obtain a polarogram;
3.检测人员依其知识和经验， 由该极谱图上的电压一 电流波形， 得知受测生物尿液中微量有机物质的种类和浓 度； 3. Based on its knowledge and experience, the tester knows the type and concentration of trace organic matter in the urine of the test organism from the voltage-current waveform on the polarogram;
4.由该 "尿液中微量有机物质的种类和浓度"来判断 该生物的生理状态。 4. The physiological state of the organism is judged by the "type and concentration of trace organic substances in the urine".
由于极谱图的波形， 不外乎是一种随时间变化的电压一电流信 号，完全可以利用电子信息科技来将之读取、记忆并储存于计算机系 统中， 因此， 将该固态电极的极谱仪， 与计算机***相连接， 通过如 RS232, USB…这一类的通讯接口，将极谱图的波形数值传送至该计算 机***内并储存于存储器中，而后，利用对比模块，将该极谱图与 "判 断标准数据"做比较，该判断标准数据是一种能据以判断该极谱图所 对应的生理状况的数字或图形。如此， 便可以利用电子信息科技， 来 自动分析检测结果， 以此来辅助， 或是取代原先的判断方法。 附图说明 Because the waveform of the polarogram is nothing more than a voltage-current signal that changes with time, it can be read, memorized and stored in a computer system by electronic information technology. Therefore, the pole of the solid-state electrode The spectrometer is connected to the computer system, and transmits the waveform value of the polarogram to the computer system through a communication interface such as RS232, USB, etc., and stores it in the memory, and then uses the comparison module to the pole The spectrum is compared with "judgment standard data", which is a number or figure from which the physiological condition corresponding to the polarogram can be judged. In this way, electronic information technology can be used to automatically analyze the test results to assist or replace the original judgment method. DRAWINGS
图 1为公知的极谱仪。 Figure 1 shows a well-known polarograph.
图 2为应用于本发明的分析尿液中微量有机物质的***的示意 图。
图 3为应用于本发明的自动分析尿液中微量有机物质的***的 2 is a schematic diagram of a system for analyzing trace organic substances in urine for use in the present invention. 3 is a system for automatically analyzing a trace amount of organic matter in urine according to the present invention.
图 4为应用于本发明的自动分析尿液中微量有机物质的***的 对比流程图。 Fig. 4 is a comparative flow chart of a system for automatically analyzing trace organic substances in urine according to the present invention.
主要部分代表标记说明 The main part represents the mark description
〔公知〕 〔known〕
01： 滴汞电极 01: Mercury drop electrode
011： 蓄汞槽 011: Mercury storage tank
012： 橡皮管 013： 毛细管 02： 甘汞电极 012: Rubber tube 013: Capillary 02: Calomel electrode
03： 电解池 04： 电压计 05： 电流计 06： 极谱仪 03: Electrolytic cell 04: Voltmeter 05: Ammeter 06: Polarograph
07： 金属电极 (参考电极） 08： 石墨电极 (工作电极） 〔本发明〕 07: Metal electrode (reference electrode) 08: Graphite electrode (working electrode) [Invention]
10： 极谱仪 31： 尿液检体
40： 信息分析装置 10: Polarograph 31: Urine sample 40: Information Analysis Device
50： 波形存取模块 50: Waveform Access Module
60： 存储器 60: memory
70： 对比模块 70: Comparison module
80： 判断标准模块 80: Judging the standard module
90： 对比结果 90: Comparison results
100： 显示器 100: display
步骤 110: 采集尿液检体的步骤 Step 110: Steps to collect urine samples
步骤 120: 将尿液检体置入极谱仪的参考电极与工作电极以产 生电压一电流信号的步骤 Step 120: Step of placing a urine sample into the reference electrode and the working electrode of the polarograph to generate a voltage-current signal
步骤 130: 将电压一电流信号传至计算机装置以产生极谱图 a 的步骤 Step 130: Steps of transmitting a voltage-current signal to a computer device to generate a polarogram a
步骤 140: 将极谱图 α记录至记忆装置内的步骤 Step 140: Steps of recording the polarogram α into the memory device
步骤.15Q: 以对比模块并依判断标准来分析极谱图 α的生理状 况的步骤 Step. 15Q: Steps to analyze the physiological state of the polarogram α by comparing the modules and judging the criteria
步骤 160: 于显示器上显示比较后的结果的步骤 具体实施方式 Step 160: Step of displaying the compared result on the display
<实施例一， 分析尿液中微量有机物质的*** > <Example 1 System for analyzing trace organic substances in urine>
如图 2所示， 该图为分析尿液中微量有机物质的***的示意图，
其中包含极谱仪 06 (将图 1中的极谱仪原有的电压、 电流计、 电压计 等等组件与电路整合于一个机壳中）， 其阳极通过导线， 连接有金属 电极 07 (参考电极）， 该极谱仪 06的阴极， 则连接有石墨电极 08 (工 作电极）。 利用本***可以捡测尿液中微量有机物质如单醣、 开放而 非环状的酮或酸类、 一些纯氨基酸例如： 半胱氨酸 （CYSTEINE)、 组 氨酸（HISTIDINE)、天门冬氨酸（ASPARTIC ACID).精氨酸（ ARGININE)、 天冬酰胺（ASPARAGINE)、 蛋氨酸（METHIONINE) 等的浓度， 同时为 利于判读， 极谱仪 06进一步可以包含连接输出装置如： 一般的打印 机或显示器等。 As shown in Figure 2, the figure is a schematic diagram of a system for analyzing trace organic matter in urine. It includes a polarograph 06 (integrating the original voltage, galvanometer, voltmeter, etc. components of the polarograph in Figure 1 into a single case), the anode of which is connected to the metal electrode 07 through a wire (Reference) Electrode), the cathode of the polarograph 06 is connected to a graphite electrode 08 (working electrode). The system can be used to detect trace amounts of organic substances in urine such as monosaccharides, open rather than cyclic ketones or acids, and some pure amino acids such as: cysteine (CYSTEINE), histidine (HISTIDINE), aspartame Acid (ASPARTIC ACID). The concentration of arginine (ARGININE), asparagine (ASPARAGINE), methionine (METHIONINE), etc., and for the convenience of interpretation, the polarograph 06 can further comprise a connection output device such as: a general printer or display Wait.
该石墨电极 08的制造方法， 经过. "除气填充工艺"的加工， 该 除气填充工艺包含有- The method for manufacturing the graphite electrode 08 is processed by a "degassing filling process", and the degassing filling process includes -
1、 除气工艺： 利用真空***将该石墨电极结构中的空气分子 除去； 1. Degassing process: removing the air molecules in the graphite electrode structure by using a vacuum system;
2、 填充工艺： 将填充物， 填入该石墨电极结构的空隙中。 此"除气填充工艺"的目的， 是除去石墨电极结构中的空隙里的 空气， 以减少这一些会干扰电解反应的分子， 另一目的是抽去这些存 在于石墨电极空隙中的空气分子，也有利于填充物去填满该石墨电极 结构上的空隙， 使空气不致于再度进入这些空隙中。 2. Filling process: The filler is filled into the gap of the graphite electrode structure. The purpose of this "degassing filling process" is to remove the air in the voids in the graphite electrode structure to reduce the molecules that interfere with the electrolytic reaction, and the other purpose is to remove the air molecules present in the voids of the graphite electrode. It is also advantageous for the filler to fill the voids in the graphite electrode structure so that the air does not enter the voids again.
<实施例二， 自动分析尿液中微量有机物质的***> <Example 2, System for automatically analyzing trace organic substances in urine>
请参见图 3，该图为自动分析尿液中微量有机物质的***。其中， 极谱仪 10， 其连接有经加工后的石墨电极 (工作电极) 08。 该石墨电
极 08的主材料为石墨， 其它亦可为玻璃石墨、 石碳膏， 以及光谱碳 等。 该石墨电极 08的外缘， 涂布有一层填充物， 于本实施例中， 该 填充物为石蜡， 其它的等同物如： 蜡、 胶水、 松脂等。 Please refer to Figure 3, which is a system for automatically analyzing trace organic matter in urine. The polarograph 10 is connected to a processed graphite electrode (working electrode) 08. The graphite electricity The main material of the pole 08 is graphite, and the others may be glass graphite, stone carbon paste, and spectral carbon. The outer edge of the graphite electrode 08 is coated with a layer of filler. In the present embodiment, the filler is paraffin, and other equivalents such as wax, glue, rosin, and the like.
金属电极 (参考电极) 07 的材料， 本实施例中采用铝， 其它等同 物如： 金、 白金、 银、 钛、 钼、 钒、 钨、 硬铝…。 该金属电极 07被 制造成杯状， 其内装有尿液检体 31。 该石墨电极 08则浸入该尿液检 体 31中（但不与该金属电极 07接触）。 The material of the metal electrode (reference electrode) 07, aluminum is used in this embodiment, and other equivalents such as: gold, platinum, silver, titanium, molybdenum, vanadium, tungsten, hard aluminum. The metal electrode 07 is formed into a cup shape in which a urine sample 31 is placed. The graphite electrode 08 is immersed in the urine sample 31 (but not in contact with the metal electrode 07).
该极谱仪 10， 与信息分析装置 40相连接， 该信息分析装置 40 中，包含有波形存取模块 50，该波形存取模块 50可以随时间的变化， 读取该极谱仪 10上的电压一电流值 (即极谱图），并将所得的极谱图， 储存于存储器 60中。 The polarimeter 10 is connected to the information analysis device 40. The information analysis device 40 includes a waveform access module 50. The waveform access module 50 can read the polarographic device 10 over time. The voltage-current value (i.e., the polarogram) is stored in the memory 60.
对比模块 70，连接于该存储器 60，可以读取该存储器 60中所储 存的极谱图， 而和判断标准模块 80中的数字或图形来做对比， 以得 到对比结果 90， 并输出该对比结果 90于显示器 100， 该显示器 100 可以是计算机屏幕、打印机， 或是有线、无线网络***， 如此便可以 把结果输出于远距的另一端点。 The comparison module 70 is connected to the memory 60, and can read the polarogram stored in the memory 60 and compare it with the number or the graphic in the judgment standard module 80 to obtain the comparison result 90, and output the comparison result. 90, in display 100, the display 100 can be a computer screen, a printer, or a wired, wireless network system, so that the results can be output to the other end of the distance.
该判断标准模块 80, 是一种能据以判断该极谱图所对应的生理 状况的分析标准，其来源是通过检测生理状况已知的受测人，而得到 的极谱图。 其取得的步骤如下所示： The criterion module 80 is an analysis standard according to which the physiological condition corresponding to the polarogram can be judged, and the source is a polarogram obtained by detecting a subject whose physiological condition is known. The steps taken are as follows:
1.找一群已确定其生理状况的受测人若干， 例如已知 其感染有特定的病毒、 或是服用有特定的药物…；
2.依标准的医学临床实验的方法， 收集该受测人的尿 液， 并以该固态电极的极谱仪检测之； 1. Find a group of subjects who have determined their physiological condition, such as being known to be infected with a specific virus, or taking a specific drug... 2. collecting the urine of the test subject according to a standard medical clinical test method, and detecting the polarogram of the solid electrode;
3.将所得的极谱图储存于该信息分析装置 40中，并分 别记录各极谱图所对应的生理状况， 以成为 "极谱图一生 3. The obtained polarographic map is stored in the information analyzing device 40, and the physiological conditions corresponding to the respective polarographic maps are recorded separately to become a "polarographic map lifetime".
. 理状况数据库"， 作为该判断标准模块 80。 The status database ", as the judgment criterion module 80.
本实施例中更具体的实施方式， 首先， 由该波形存取模块 50， 于每 0. 001秒读取一次该极谱仪 10所测得的极谱图，一共读取 5秒， 总共 5000个电压一电流值。 该 5000个数据， 即为尿液检体 31的极 谱图波形数据， 为描述上的便利起见， 我们简称之为极谱图 α。 In a more specific embodiment of the present embodiment, first, the waveform access module 50 reads the polarogram measured by the polarimeter 10 every 0.001 seconds, and reads a total of 5 seconds, for a total of 5000. Voltage-current value. The 5000 data, which is the polarographic waveform data of the urine sample 31, is simply referred to as the polarogram α for the convenience of description.
该判断标准模块 80中， 记录有各种生理状况的极谱图 Ν张。 对 比模块 70， 其功能用于比较该极谱图 α 和 "判断标准模块 80中的 波形数据"， 简单的说就像是 "查阅索引"。 请参考图 4， 为本发明实 施例的对比流程图，首先采集尿液检体 31 (步骤 110)，并将该尿液检 体 31置入至该极谱仪 06的参考电极 07与工作电极 08浸入于该尿液 检体 31中， 而产生电压一电流信号 (步骤 120)， 该电压一电流信号 通过极谱仪 10传送至计算机装置， 产生极谱图 α (步骤 130)， 并将 该极谱图 α记录至记忆装置内（步骤 140) , 以对比模块 70依判断标 准模块 80， 来分析该极谱图 α辨别受检生物的生理状况 (步骤 150)， 假设该极谱图 α ,被该对比模块 70判断为与该判断标准模块 80中的 第 X个波形数据相似，而该第 X个波形数据，来自于具有某特定异常 生理状况的生物体的尿液检体， 如此， 则代表该尿液检体 31的提供 者 Ρ (受测者 Ρ)， 亦应有同样于该异常生物体的生理状况， 最后于显
示器显示比较后的结果 (步骤 160) 。 In the judgment criterion module 80, polarographic maps of various physiological conditions are recorded. The comparison module 70 has a function for comparing the polarogram α with "the waveform data in the judgment standard module 80", which is simply referred to as "reference index". Please refer to FIG. 4, which is a comparative flowchart of an embodiment of the present invention. First, the urine sample 31 is collected (step 110), and the urine sample 31 is placed into the reference electrode 07 and the working electrode of the polarograph 06. 08 is immersed in the urine sample 31 to generate a voltage-current signal (step 120), and the voltage-current signal is transmitted to the computer device through the polarimeter 10 to generate a polarogram α (step 130), and the The polarogram α is recorded into the memory device (step 140), and the comparison module 70 analyzes the polarographic map α to determine the physiological condition of the detected organism according to the criterion module 80 (step 150), assuming the polarogram α, It is determined by the comparison module 70 that it is similar to the X-th waveform data in the determination criterion module 80, and the X-th waveform data is from a urine sample of a living body having a certain abnormal physiological condition, and thus, The provider Ρ (subject Ρ) representing the urine sample 31 should also have the same physiological condition as the abnormal organism, and finally The display shows the result of the comparison (step 160).
该对比模块 70具体的比较方法， 于本实施例中， 其程序为： The comparison module 70 has a specific comparison method. In this embodiment, the program is:
1.由存储器 60中， 读取该极谱图 α； 1. From the memory 60, reading the polarogram α;
2.将该极谱图 α的 5000.个数据， 与该判断标准模块 80中的第 1张极谱图的 5000数据分别相减， 并分别取绝 对值。 如， 取该极谱图 α的第 1个数据， 与该判断标准模 块 80中的第 1张极谱图的第 1个电压值相减后， 取得第 1 个绝对值， 如此重复至做完 5000个电压值数据的相减和， 和取得 5000个绝对值； 2. The 5000 data of the polarogram α is subtracted from the 5000 data of the first polarographic map in the judgment standard module 80, and the absolute values are respectively taken. For example, the first data of the polarogram α is subtracted from the first voltage value of the first polarogram in the determination standard module 80, and then the first absolute value is obtained, and thus the repetition is completed. Subtracting the sum of 5000 voltage value data, and obtaining 5000 absolute values;
3.将该 5000个绝对值相加， 记为第 1个差异值；3. Add the 5000 absolute values and record the first difference value;
4.重复第 2、 第 3步骤， 直至比较完该判断标准模块 80中全部的极谱图。此时，若该判断标准模块 80中有 1000 张极谱图， 就有 1000个差异值数据，各自对应于该判断标 准模块 80中的 1000张极谱图； 4. Repeat steps 2 and 3 until all polarographs in the judgment standard module 80 are compared. At this time, if there are 1000 polarograms in the judgment standard module 80, there are 1000 difference value data, which respectively correspond to 1000 polarograms in the judgment standard module 80;
5.将各个差异值， 依大小排序； 5. Sort each difference value by size;
6.取差异值最小的 10个，并将其所对应于该判断标准 模块 80中的极谱图，连同所对应的生理状况，传送至对比 结果 90; 6. Take the 10 smallest difference values, and transmit the polarographic map corresponding to the judgment standard module 80, together with the corresponding physiological condition, to the comparison result 90;
7.显示该对比结果 90。 7. Display the comparison result 90.
本实施例中， 该信息分析装置 40中的各种模块， 可以为硬件也 可以为软件，这点对信息领域的人士而言，都是用熟悉的技术便可实
现的。 In this embodiment, various modules in the information analysis device 40 may be hardware or software, which is true to those in the information field by using familiar techniques. Now.
对比模块 70的对比方法，于本实施例中采用绝对值相加的方法。 然而， 在数学运算上， 判断差异大小的方式并非仅此一种， 例如将相 减的差值取平方数后再相加的方法，亦为所属技术领域的技术人员所 熟知， 应视为本发明的等同范围。 In the comparison method of the comparison module 70, the absolute value addition method is employed in this embodiment. However, in mathematical operations, the manner of judging the magnitude of the difference is not the only one. For example, the method of taking the subtracted difference by the square number and then adding them is also well known to those skilled in the art, and should be regarded as The equivalent scope of the invention.
判断标准模块 80中的数据， 亦可以是各种生理状况的极谱图的 特征， 而非全图， 对比模块 70在进行对比时， 便可针对这一部分的 特征来进行， 以更精确地进行分析。 The data in the criterion module 80 can also be a feature of the polarogram of various physiological conditions, rather than a full map. When comparing the comparison module 70, the comparison can be performed on the features of this part for more precise analysis.
另外，运用本发明，还可以利用网络信息技术作大规模及区域性 的生物体测量与信息统计工作，以了解与分析群体生物体的异常生理 状况。 In addition, with the present invention, network information technology can also be utilized for large-scale and regional biometrics and information statistics to understand and analyze abnormal physiological conditions of group organisms.
以上所述仅为本发明的较佳实施例而已，并非用以限定本发明的 实施范围。所属技术领域的技术人员应明白各种不同的实施例与变化 得在不脱离本发明的总的思想与范围下施行，本发明的范围乃由权利 要求所限定，与本发明的权利要求意义相等及在权利要求范围之内所 做的各种修改均被视为包含于本发明的权利要求内。
The above is only the preferred embodiment of the present invention and is not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that the various embodiments and variations may be practiced without departing from the scope of the inventions. And various modifications made within the scope of the claims are deemed to be included in the claims of the invention.
Claims
1. 一种分析尿液中微量有机物质的***， 其特征是至少包含： 极谱仪， 具有至少一个电路连接端； A system for analyzing trace organic matter in urine, characterized in that it comprises at least: a polarograph having at least one circuit connection end;
石墨电极,通过导线连接于该极谱仪的一连接端，作为工作电极; 金属电极,通过导线连接于该极谱仪的一连接端，作为参考电极; 及 a graphite electrode connected to a connection end of the polarimeter as a working electrode; a metal electrode connected to a connection end of the polarimeter as a reference electrode; and
信息分析装置，连接于该极谱仪的一电路连接端，用以输出自动 分析的信息结果。 The information analysis device is connected to a circuit connection end of the polarimeter for outputting the information result of the automatic analysis.
2..根据权利要求 1所述的分析尿液中微量有机物质的***， 其 特征是该信息分析装置进一步包含有 - 波形存取模块，连接于该极谱仪的输出端，可读取储存该极谱仪 所测得的极谱图 α ; 2. The system for analyzing trace organic matter in urine according to claim 1, wherein the information analysis device further comprises a waveform access module connected to the output of the polarimeter for reading and storing. The polarogram α measured by the polarograph;
存储器， 连接于该波形存取模块， 可储存该极谱图 α； 判断标准模块，存有可据以判断该极谱图 α所对应的生理状况的 标准； a memory connected to the waveform access module for storing the polarogram α; a criterion module having a criterion for determining a physiological condition corresponding to the polarogram α;
对比模块，连接于该存储器和该判断标准模块，可以读取该存储 器和该判断标准模块中的数据， 以进行对比， 并输出对比结果； 及 . 显示器， 可以显示该对比结果。 The comparison module is connected to the memory and the judgment standard module, and can read the data in the memory and the judgment standard module for comparison, and output a comparison result; and a display, which can display the comparison result.
3.根据权利要求 1所述的分析尿液中微量有机物质的***， 其 特征是该石墨电极的材料是选用自一般石墨、玻璃石墨、石碳膏及光 谱碳。
3. The system for analyzing trace organic matter in urine according to claim 1, wherein the material of the graphite electrode is selected from the group consisting of general graphite, glass graphite, stone carbon paste and spectral carbon.
4. 根据权利要求 1所述的分析尿液中微量有机物质的***， 其 特征是该金属电极的材料是选用自铝、 金、 白金、 银、 钛、 钼、 钒、 钨、 硬铝及合金。 4. The system for analyzing trace organic matter in urine according to claim 1, wherein the material of the metal electrode is selected from the group consisting of aluminum, gold, platinum, silver, titanium, molybdenum, vanadium, tungsten, hard aluminum and alloy. .
5.根据权利要求 2所述的分析尿液中微量有机物质的***， 其 特征是该判断标准模块中的数据，为由已知生理状况的受测者的极谱 图数据所建立。 A system for analyzing trace organic substances in urine according to claim 2, wherein the data in the judgment criterion module is established by polarographic data of a subject having a known physiological condition.
6. 一种分析检测尿液中微量有机物质的方法， 其特征是使用改 进的极谱仪与相对的数据分析技术， 其分析步骤包含： 6. A method for the analysis of trace amounts of organic matter in urine, characterized by the use of an improved polarograph and relative data analysis techniques, the analysis steps of which include:
釆集尿液检体； Collect urine samples;
将该尿液检体加入至该极谱仪的参考电极及将该极谱仪的工作 电极浸入于该尿液检体中，使得工作电极与该参考电极之间产生电压 一电流信号； Adding the urine sample to the reference electrode of the polarimeter and immersing the working electrode of the polarograph in the urine sample, so that a voltage-current signal is generated between the working electrode and the reference electrode;
将该电压一电流信号通过极谱仪传送至计算机装置，产生极谱图 α； Passing the voltage-current signal to the computer device through the polarograph to generate a polarogram α;
将该极谱图 α记录至记忆装置内； Recording the polarogram α into the memory device;
以对比模块，依判断标准，来分析该极谱图 α辨别受检生物的生 理状况； 及 Using the comparison module, according to the judgment standard, analyzing the polarographic map α to distinguish the biological condition of the tested organism;
于显示器显示比较后的结果。 The result of the comparison is displayed on the display.
7.根据权利要求 6所述的分析检测尿液中微量有机物质的方法, 其特征是该以对比模块，依判断标准，来分析该极谱图 α辨别受检生 物的生理状况的步骤进一步包含： The method for analyzing and detecting trace organic substances in urine according to claim 6, characterized in that the step of analyzing the polarographic map α to distinguish the physiological condition of the test organism according to the judgment module further comprises :
由该存储器中， 读取由该波形存取模块所记录的该极谱图 α ;
将该极谱图 α，与该判断标准模块中的数据分别比较， 以得出差 异值； 及 Reading, by the memory, the polarogram α recorded by the waveform access module ; Comparing the polarogram α with the data in the judgment standard module to obtain a difference value;
取至少一个， 差异值最小的波形数据， 作为比较的结果。 Take at least one waveform data with the smallest difference value as the result of the comparison.
8. 根据权利要求 6所述的分析检测尿液中微量有机物质的方法， 其特征是该以对比模块，依判断标准，来分析该极谱图 α辨别受检生 物的生理状况的步骤进一步包含： 8. The method for analyzing and detecting trace organic substances in urine according to claim 6, wherein the step of analyzing the polarographic map α to determine the physiological condition of the biological organism according to the judgment module further comprises: :
该对比模块读取该记忆装置中的极谱图 α，并与判断标准模块中 的多张极谱图分别相减并取差异的绝对值； 及 The comparison module reads the polarogram α in the memory device and subtracts the multiple polarograms in the standard module and takes the absolute value of the difference;
选出差异最小的一个， 作为对比的结果。 Select the one with the smallest difference as a result of the comparison.
9. 一种分析尿液中微量有机物质***的检测装置， 其特征是至 少包含： 9. A detection device for analyzing a system of trace organic substances in urine, characterized in that it comprises at least:
极谱仪， 具有至少一个连接端； a polarograph having at least one connection end;
石墨电极，通过导线连接于该极谱仪的一连接端，作为工作电极； 及 a graphite electrode connected to a connection end of the polarimeter as a working electrode;
金属电极，通过导线连接于该极谱仪的一连接端，作为参考电极。 A metal electrode is connected to a connection end of the polarimeter by a wire as a reference electrode.
10.根据权利要求 9所述的分析尿液中微量有机物质***的检测 装置， 其特征是该极谱仪进一步包含连接有输出装置。
10. The apparatus for detecting a trace organic matter system in urine according to claim 9, wherein the polar spectrometer further comprises an output device connected thereto.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/988,097 US8092669B2 (en) | 2005-07-14 | 2005-07-14 | System and method of examining trace organic matters in living being's urine |
| CNA2005800502562A CN101208600A (en) | 2005-07-14 | 2005-07-14 | System and method for analyzing trace organic substance in biological urine |
| PCT/CN2005/001044 WO2007006180A1 (en) | 2005-07-14 | 2005-07-14 | A system for analyzing trace quantity of organic substances in urine and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2005/001044 WO2007006180A1 (en) | 2005-07-14 | 2005-07-14 | A system for analyzing trace quantity of organic substances in urine and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2007006180A1 true WO2007006180A1 (en) | 2007-01-18 |
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| PCT/CN2005/001044 WO2007006180A1 (en) | 2005-07-14 | 2005-07-14 | A system for analyzing trace quantity of organic substances in urine and its application |
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| Country | Link |
|---|---|
| US (1) | US8092669B2 (en) |
| CN (1) | CN101208600A (en) |
| WO (1) | WO2007006180A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699448A (en) * | 2016-01-14 | 2016-06-22 | 京东方科技集团股份有限公司 | Urine detection method and device |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548326B (en) * | 2015-12-10 | 2017-12-22 | 苏州国环环境检测有限公司 | Organic substance detection method in a kind of waste water |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3937577A1 (en) * | 1989-11-11 | 1991-05-16 | Lutz Ehrlich | Voltametric analysis cell - with holder cavity holding oversize mercury droplet |
| JPH08304336A (en) * | 1995-05-10 | 1996-11-22 | Toto Ltd | Polarographic urine analyzing method and device therefor |
| CN1291722A (en) * | 2000-12-12 | 2001-04-18 | 天津市超净过滤技术研究所 | Method for measuring trace elements by pulse polarography |
| US20010045357A1 (en) * | 1999-06-08 | 2001-11-29 | Scott Broadley | Novel reference electrode having a microfluidic flowing liquid junction |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3922205A (en) * | 1973-08-20 | 1975-11-25 | Dow Chemical Co | Portable polarographic analyzer and quick polarographic determinations |
| USRE29703E (en) * | 1974-01-17 | 1978-07-18 | The Regents Of The University Of California | Non-invasively measuring arterial oxygen tension |
| GB2216267A (en) * | 1988-03-28 | 1989-10-04 | Cubio Ltd | Paracetamol sensor |
-
2005
- 2005-07-14 WO PCT/CN2005/001044 patent/WO2007006180A1/en active Application Filing
- 2005-07-14 US US11/988,097 patent/US8092669B2/en not_active Expired - Fee Related
- 2005-07-14 CN CNA2005800502562A patent/CN101208600A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3937577A1 (en) * | 1989-11-11 | 1991-05-16 | Lutz Ehrlich | Voltametric analysis cell - with holder cavity holding oversize mercury droplet |
| JPH08304336A (en) * | 1995-05-10 | 1996-11-22 | Toto Ltd | Polarographic urine analyzing method and device therefor |
| US20010045357A1 (en) * | 1999-06-08 | 2001-11-29 | Scott Broadley | Novel reference electrode having a microfluidic flowing liquid junction |
| CN1291722A (en) * | 2000-12-12 | 2001-04-18 | 天津市超净过滤技术研究所 | Method for measuring trace elements by pulse polarography |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699448A (en) * | 2016-01-14 | 2016-06-22 | 京东方科技集团股份有限公司 | Urine detection method and device |
| CN105699448B (en) * | 2016-01-14 | 2018-02-06 | 京东方科技集团股份有限公司 | A kind of urine detection method and urine detector |
| US10261070B2 (en) | 2016-01-14 | 2019-04-16 | Boe Technology Group Co., Ltd. | Urine detection method and urine detection device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101208600A (en) | 2008-06-25 |
| US8092669B2 (en) | 2012-01-10 |
| US20090152131A1 (en) | 2009-06-18 |
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