WO2006071895A3 - Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences - Google Patents

Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences Download PDF

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Publication number
WO2006071895A3
WO2006071895A3 PCT/US2005/047143 US2005047143W WO2006071895A3 WO 2006071895 A3 WO2006071895 A3 WO 2006071895A3 US 2005047143 W US2005047143 W US 2005047143W WO 2006071895 A3 WO2006071895 A3 WO 2006071895A3
Authority
WO
WIPO (PCT)
Prior art keywords
electrodes
dna sequence
recognition
target dna
polynucleotide sequences
Prior art date
Application number
PCT/US2005/047143
Other languages
French (fr)
Other versions
WO2006071895A2 (en
Inventor
Jean-Christophe P Gabriel
Shripal Gandhi
George Gruner
Charles Steven Joiner Jr
Ron Sosnowski
Alexander Star
Eugene Tu
Christian Valcke
Original Assignee
Nanomix Inc
Niemann Joseph
Jean-Christophe P Gabriel
Shripal Gandhi
George Gruner
Charles Steven Joiner Jr
Ron Sosnowski
Alexander Star
Eugene Tu
Christian Valcke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/212,026 external-priority patent/US20070178477A1/en
Priority claimed from US11/274,747 external-priority patent/US20070208243A1/en
Application filed by Nanomix Inc, Niemann Joseph, Jean-Christophe P Gabriel, Shripal Gandhi, George Gruner, Charles Steven Joiner Jr, Ron Sosnowski, Alexander Star, Eugene Tu, Christian Valcke filed Critical Nanomix Inc
Priority to EP05855662A priority Critical patent/EP1831670A4/en
Priority to JP2007549560A priority patent/JP2008525822A/en
Publication of WO2006071895A2 publication Critical patent/WO2006071895A2/en
Publication of WO2006071895A3 publication Critical patent/WO2006071895A3/en
Priority to US14/961,572 priority patent/US20160187276A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
    • H01L2924/00—Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
    • H01L2924/0001—Technical content checked by a classifier
    • H01L2924/0002—Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00

Abstract

A nanotube device is configured as an electronic sensor for a target DNA sequence. A film of nanotubes is deposited over electrodes on a substrate. A solution of single-strand DNA is prepared so as to be complementary to a target DNA sequence. The DNA solution is deposited over the electrodes, dried, and removed from the substrate except in a region between the electrodes. The resulting structure includes strands of the desired DNA sequence in direct contact with nanotubes between opposing electrodes, to form a sensor that is electrically responsive to the presence of target DNA strands. Alternative assay embodiments are described which employ linker groups to attach ssDNA probes to the nanotube sensor device.
PCT/US2005/047143 2004-05-14 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences WO2006071895A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP05855662A EP1831670A4 (en) 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences
JP2007549560A JP2008525822A (en) 2004-12-28 2005-12-23 Nanoelectronic device for DNA detection / recognition of polynucleotide sequences
US14/961,572 US20160187276A1 (en) 2004-05-14 2015-12-07 Electrochemical nanosensors for biomolecule detection

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US63995404P 2004-12-28 2004-12-28
US60/639,954 2004-12-28
US65727505P 2005-02-28 2005-02-28
US60/657,275 2005-02-28
US66887905P 2005-04-05 2005-04-05
US60/668,879 2005-04-05
US11/212,026 2005-08-24
US11/212,026 US20070178477A1 (en) 2002-01-16 2005-08-24 Nanotube sensor devices for DNA detection
US73090505P 2005-10-27 2005-10-27
US60/730,905 2005-10-27
US11/274,747 2005-11-15
US11/274,747 US20070208243A1 (en) 2002-01-16 2005-11-15 Nanoelectronic glucose sensors
US73869405P 2005-11-21 2005-11-21
US60/738,694 2005-11-21
US74883405P 2005-12-09 2005-12-09
US60/748,834 2005-12-09

Publications (2)

Publication Number Publication Date
WO2006071895A2 WO2006071895A2 (en) 2006-07-06
WO2006071895A3 true WO2006071895A3 (en) 2007-02-08

Family

ID=36615493

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/047143 WO2006071895A2 (en) 2004-05-14 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Country Status (3)

Country Link
EP (1) EP1831670A4 (en)
JP (1) JP2008525822A (en)
WO (1) WO2006071895A2 (en)

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US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection
US9103775B2 (en) 2002-01-16 2015-08-11 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US9291613B2 (en) 2002-06-21 2016-03-22 Nanomix, Inc. Sensor having a thin-film inhibition layer

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JP2008511008A (en) * 2004-08-24 2008-04-10 ナノミックス・インコーポレーテッド Nanotube sensor device for DNA detection
EP2007379A4 (en) * 2006-04-04 2009-12-09 Nanomix Inc Nanoelectronic detection of biomolecules employing analyte amplification and reporters
ES2289948B1 (en) * 2006-07-19 2008-09-16 Starlab Barcelona, S.L. ELECTROPHYSIOLOGICAL SENSOR.
JP5061342B2 (en) * 2006-09-11 2012-10-31 国立大学法人大阪大学 Carbon nanotube electrode and sensor using the electrode
US8465634B2 (en) * 2007-05-23 2013-06-18 Arizona Board Of Regents Systems and methods for integrated electrochemical and electrical detection
CN101939329A (en) * 2007-08-30 2011-01-05 滑铁卢大学 Amino acid pairing-based self assembling peptides and methods
WO2010005080A1 (en) * 2008-07-10 2010-01-14 株式会社イデアルスター Gas sensor
CA2739370A1 (en) 2008-10-02 2010-04-08 University Of New Haven Bionanosensor detection device
GB0822733D0 (en) * 2008-12-12 2009-01-21 Univ Warwick Nanotube electrochemistry
JP2011220803A (en) * 2010-04-08 2011-11-04 Mitsumi Electric Co Ltd Biosensor including field-effect transistor element
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9187783B2 (en) 2010-10-04 2015-11-17 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
CN103717753B (en) * 2011-05-27 2016-12-07 吉纳普赛斯股份有限公司 For heredity and the system and method for bioanalysis
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
RU2638132C2 (en) 2012-10-16 2017-12-11 Конинклейке Филипс Н.В. Integrated circuit with nano-conductive sensors in field transistors, manufactured by chemical method, sensor device, method of measurement and method of manufacture
CN102938397B (en) * 2012-12-05 2015-09-09 苏州纳格光电科技有限公司 Electronic device and preparation method thereof
CN105051214B (en) 2013-03-15 2018-12-28 吉纳普赛斯股份有限公司 System and method for bioanalysis
US9683957B2 (en) * 2013-05-29 2017-06-20 Csir Field effect transistor and a gas detector including a plurality of field effect transistors
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
CN105980581B (en) * 2013-12-12 2021-03-26 阿尔查技术有限公司 Capacitive sensor and method of use
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
WO2016168734A1 (en) * 2015-04-15 2016-10-20 Malcolm Alastair J Methods of manufacturing semiconductor arrays
WO2017033922A1 (en) * 2015-08-24 2017-03-02 国立大学法人 東京大学 Method of detecting target substance using field-effect transistor
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
WO2019060628A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
US20190219512A1 (en) * 2018-01-12 2019-07-18 Clifford H. Kern, III Device, procedure and system for detecting bacterial pathogens including methicillin-resistant staphylococcus aureus or clostridium difficile
CN113588605A (en) * 2021-06-18 2021-11-02 山东师范大学 Method for detecting multiple environmental estrogens and application thereof
US11845079B2 (en) * 2021-12-08 2023-12-19 International Business Machines Corporation Integrated silicon platform for electronic biosensors

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Publication number Priority date Publication date Assignee Title
US9103775B2 (en) 2002-01-16 2015-08-11 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US9291613B2 (en) 2002-06-21 2016-03-22 Nanomix, Inc. Sensor having a thin-film inhibition layer
US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection

Also Published As

Publication number Publication date
EP1831670A2 (en) 2007-09-12
EP1831670A4 (en) 2010-09-15
JP2008525822A (en) 2008-07-17
WO2006071895A2 (en) 2006-07-06

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