WO2006040104A2 - Sequencing of nucleic acids with strand displacement - Google Patents

Sequencing of nucleic acids with strand displacement Download PDF

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Publication number
WO2006040104A2
WO2006040104A2 PCT/EP2005/010869 EP2005010869W WO2006040104A2 WO 2006040104 A2 WO2006040104 A2 WO 2006040104A2 EP 2005010869 W EP2005010869 W EP 2005010869W WO 2006040104 A2 WO2006040104 A2 WO 2006040104A2
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
nucleic acid
strand displacement
nucleic acids
strand
Prior art date
Application number
PCT/EP2005/010869
Other languages
German (de)
French (fr)
Other versions
WO2006040104A3 (en
Inventor
Achim Fischer
Original Assignee
Achim Fischer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Achim Fischer filed Critical Achim Fischer
Publication of WO2006040104A2 publication Critical patent/WO2006040104A2/en
Publication of WO2006040104A3 publication Critical patent/WO2006040104A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for nucleic acid sequencing, comprising the steps: (a) preparation of a nucleic acid for sequencing in immobilised and at least partly double-stranded form, (b) hybridisation of a sequencing primer on one of the strands of the nucleic acid, (c) sequencing of at least a section of the nucleic acid with extension of the sequencing primer and strand displacement of that nucleic acid strand which was present in hybridised form on the primer binding strand.

Description

       

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Claims

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PCT/EP2005/010869 2004-10-09 2005-10-08 Sequencing of nucleic acids with strand displacement WO2006040104A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200410049532 DE102004049532A1 (en) 2004-10-09 2004-10-09 Sequencing of Nucleic Acids via Beach Displacement
DE102004049532.7 2004-10-09

Publications (2)

Publication Number Publication Date
WO2006040104A2 true WO2006040104A2 (en) 2006-04-20
WO2006040104A3 WO2006040104A3 (en) 2006-07-20

Family

ID=35355917

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010869 WO2006040104A2 (en) 2004-10-09 2005-10-08 Sequencing of nucleic acids with strand displacement

Country Status (2)

Country Link
DE (1) DE102004049532A1 (en)
WO (1) WO2006040104A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2453023A3 (en) * 2006-10-06 2012-08-22 Illumina Cambridge Limited Method for sequencing a polynucleotide template

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5981190A (en) * 1997-01-08 1999-11-09 Ontogeny, Inc. Analysis of gene expression, methods and reagents therefor
AU2001254771A1 (en) * 2000-04-03 2001-10-15 Axaron Bioscience Ag Novel method for the parallel sequencing of a nucleic acid mixture on a surface

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2453023A3 (en) * 2006-10-06 2012-08-22 Illumina Cambridge Limited Method for sequencing a polynucleotide template
US8431348B2 (en) 2006-10-06 2013-04-30 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
US8765381B2 (en) 2006-10-06 2014-07-01 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
US9267173B2 (en) 2006-10-06 2016-02-23 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
US10221452B2 (en) 2006-10-06 2019-03-05 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
EP3670672A1 (en) * 2006-10-06 2020-06-24 Illumina Cambridge Limited Method for sequencing a polynucleotide template

Also Published As

Publication number Publication date
DE102004049532A1 (en) 2006-04-13
WO2006040104A3 (en) 2006-07-20

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