WO2006024788A8 - Procede et dispositif de mesure d'une activite enzymatique dans un fluide biologique - Google Patents

Procede et dispositif de mesure d'une activite enzymatique dans un fluide biologique

Info

Publication number
WO2006024788A8
WO2006024788A8 PCT/FR2005/002031 FR2005002031W WO2006024788A8 WO 2006024788 A8 WO2006024788 A8 WO 2006024788A8 FR 2005002031 W FR2005002031 W FR 2005002031W WO 2006024788 A8 WO2006024788 A8 WO 2006024788A8
Authority
WO
WIPO (PCT)
Prior art keywords
measuring
body fluid
enzymatic activity
substrate
electroactive
Prior art date
Application number
PCT/FR2005/002031
Other languages
English (en)
Other versions
WO2006024788A1 (fr
Inventor
Jean-Maxime Nigretto
Guillaume Lamblin
Marielle Paris
Norbert Benattar
Original Assignee
Biopep
Univ Cergy Pontoise
Jean-Maxime Nigretto
Guillaume Lamblin
Marielle Paris
Norbert Benattar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biopep, Univ Cergy Pontoise, Jean-Maxime Nigretto, Guillaume Lamblin, Marielle Paris, Norbert Benattar filed Critical Biopep
Priority to EP05796245A priority Critical patent/EP1781804A1/fr
Priority to US11/573,797 priority patent/US20070269854A1/en
Priority to JP2007526506A priority patent/JP2008509693A/ja
Publication of WO2006024788A1 publication Critical patent/WO2006024788A1/fr
Publication of WO2006024788A8 publication Critical patent/WO2006024788A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé et un dispositif pour mesurer une activité enzymatique dans un fluide biologique. Le procédé comprend les opérations suivantes : a) fournir un support présentant une surface conductrice sur laquelle est immobilisé un substrat spécifique de l'activité enzymatique à mesurer, le substrat comportant un résidu électroactif ; b) faire réagir un échantillon du fluide biologique avec le substrat de manière à provoquer une réaction enzymatique engendrant un produit électroactif ; c) détecter la quantité du produit électroactif par une mesure ampérométrique au moyen du support en contact avec le milieu contenant l'enzyme. Application notamment à la mesure d'activités enzymatiques liées à la coagulation du sang.
PCT/FR2005/002031 2004-08-18 2005-08-05 Procede et dispositif de mesure d'une activite enzymatique dans un fluide biologique WO2006024788A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP05796245A EP1781804A1 (fr) 2004-08-18 2005-08-05 Procede et dispositif de mesure d'une activite enzymatique dans un fluide biologique
US11/573,797 US20070269854A1 (en) 2004-08-18 2005-08-05 Method and Device for Measuring an Enzymatic Activity in a Body Fluid
JP2007526506A JP2008509693A (ja) 2004-08-18 2005-08-05 体液中の酵素活性を測定するための方法及び装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0408960A FR2874385B1 (fr) 2004-08-18 2004-08-18 Procede et dispositif de mesure d'une activite enzymatique dans un fluide biologique
FR0408960 2004-08-18

Publications (2)

Publication Number Publication Date
WO2006024788A1 WO2006024788A1 (fr) 2006-03-09
WO2006024788A8 true WO2006024788A8 (fr) 2007-03-15

Family

ID=34950074

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2005/002031 WO2006024788A1 (fr) 2004-08-18 2005-08-05 Procede et dispositif de mesure d'une activite enzymatique dans un fluide biologique

Country Status (5)

Country Link
US (1) US20070269854A1 (fr)
EP (1) EP1781804A1 (fr)
JP (1) JP2008509693A (fr)
FR (1) FR2874385B1 (fr)
WO (1) WO2006024788A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI516601B (zh) * 2007-10-26 2016-01-11 環球生物醫療感測器私人有限公司 電化學檢測之裝置及方法
CA2880104A1 (fr) * 2012-07-27 2014-01-30 Ohmx Corporation Mesure electrique de monocouches apres detection pro-clivage de la presence et de l'activite d'enzymes et d'autres analytes cibles
US11760170B2 (en) 2020-08-20 2023-09-19 Denso International America, Inc. Olfaction sensor preservation systems and methods
US11760169B2 (en) 2020-08-20 2023-09-19 Denso International America, Inc. Particulate control systems and methods for olfaction sensors
US11828210B2 (en) 2020-08-20 2023-11-28 Denso International America, Inc. Diagnostic systems and methods of vehicles using olfaction
US11881093B2 (en) 2020-08-20 2024-01-23 Denso International America, Inc. Systems and methods for identifying smoking in vehicles
US11932080B2 (en) 2020-08-20 2024-03-19 Denso International America, Inc. Diagnostic and recirculation control systems and methods
US11636870B2 (en) 2020-08-20 2023-04-25 Denso International America, Inc. Smoking cessation systems and methods
US11813926B2 (en) 2020-08-20 2023-11-14 Denso International America, Inc. Binding agent and olfaction sensor

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4140662A (en) * 1977-03-25 1979-02-20 Ortho Diagnostics, Inc. Attachment of proteins to inert particles
EP0018002B1 (fr) * 1979-04-24 1983-02-09 Marcel Jozefonvicz Procédé pour la détermination de protéases et d'anti-protéases
US4379780A (en) * 1980-09-29 1983-04-12 American Home Products Corporation 17 α-Dihydroequilin hapten and assay method
US4680338A (en) * 1985-10-17 1987-07-14 Immunomedics, Inc. Bifunctional linker
US5491097A (en) * 1989-06-15 1996-02-13 Biocircuits Corporation Analyte detection with multilayered bioelectronic conductivity sensors
US6126899A (en) * 1996-04-03 2000-10-03 The Perkins-Elmer Corporation Device for multiple analyte detection
EP1157029B1 (fr) * 1999-02-23 2005-12-28 Pentapharm AG Derives d'oligopeptides pour la mesure electrochimique de l'activite de proteases
EP2042610A1 (fr) * 1999-02-23 2009-04-01 Asulab S.A. Système électrochimique pour la détermination d'un temps de coagulation du sang
AU1763401A (en) * 1999-11-15 2001-05-30 I-Stat Corporation Apparatus and method for assaying coagulation in fluid samples
CA2400651A1 (fr) * 2000-02-21 2001-08-30 F. Hoffmann-La Roche Ag Capteur electrochimique permettant de determiner la coagulation sanguine, systeme correspondant de mesure de la coagulation sanguine et procede permettant de determiner la coagulation sanguine
JP4435454B2 (ja) * 2001-09-03 2010-03-17 富士フイルム株式会社 バイオセンサー用表面

Also Published As

Publication number Publication date
US20070269854A1 (en) 2007-11-22
EP1781804A1 (fr) 2007-05-09
JP2008509693A (ja) 2008-04-03
FR2874385A1 (fr) 2006-02-24
WO2006024788A1 (fr) 2006-03-09
FR2874385B1 (fr) 2006-12-08

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