WO2006017741A3 - Radiation inducible iex-1 promoter - Google Patents

Radiation inducible iex-1 promoter Download PDF

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Publication number
WO2006017741A3
WO2006017741A3 PCT/US2005/027906 US2005027906W WO2006017741A3 WO 2006017741 A3 WO2006017741 A3 WO 2006017741A3 US 2005027906 W US2005027906 W US 2005027906W WO 2006017741 A3 WO2006017741 A3 WO 2006017741A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
promoter
iex
radiation
useful
Prior art date
Application number
PCT/US2005/027906
Other languages
French (fr)
Other versions
WO2006017741A2 (en
Inventor
Mira Jung
Anatoly Dritschilo
Original Assignee
Univ Georgetown
Mira Jung
Anatoly Dritschilo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgetown, Mira Jung, Anatoly Dritschilo filed Critical Univ Georgetown
Publication of WO2006017741A2 publication Critical patent/WO2006017741A2/en
Publication of WO2006017741A3 publication Critical patent/WO2006017741A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

DNA molecules, such as expression vectors, comprising a radiation-inducible IEX-1 promoter operably coupled to a sequence encoding a biologically-active protein or polypeptide are useful for gene transfer applications. Preferably, the promoter is an IEX-1 promoter that is modified to provide increased radiation response and or specificity. Methods for delivery of such a DNA expression vector to tissues of a host animal, and other methods of using such a molecule are described. The vectors and methods are useful for research purposes, for example, for the controlled modification and study of cellular response to radiation using in vitro and in vivo methods. The vectors and methods are also useful in, and relate to, therapeutic compositions and methods, for example in the treatment of cancers, such as prostate cancer.
PCT/US2005/027906 2004-08-06 2005-08-05 Radiation inducible iex-1 promoter WO2006017741A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59902804P 2004-08-06 2004-08-06
US60/599,028 2004-08-06

Publications (2)

Publication Number Publication Date
WO2006017741A2 WO2006017741A2 (en) 2006-02-16
WO2006017741A3 true WO2006017741A3 (en) 2006-09-14

Family

ID=35839943

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/027906 WO2006017741A2 (en) 2004-08-06 2005-08-05 Radiation inducible iex-1 promoter

Country Status (1)

Country Link
WO (1) WO2006017741A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2022163653A1 (en) * 2021-01-29 2022-08-04

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156736A (en) * 1990-12-20 2000-12-05 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156736A (en) * 1990-12-20 2000-12-05 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
IM ET AL.: "Divergent regulation of the growth-promoting gene IEX-1 by the p53 tumor suppressor and Sp1", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 17, 26 April 2002 (2002-04-26), pages 14612 - 14621, XP003001922 *
KONDRATYEV ET AL.: "Identification and characterization of a radiation-inducible glycosylated human early-response gene", CANCER RESEARCH, vol. 56, 1 April 1996 (1996-04-01), pages 1498 - 1502, XP001247067 *
KUMAR ET AL.: "A novel immediate early response gene, IEX-1, is induced by ultraviolet radiation in human keratinocytes", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 253, 1998, pages 336 - 341, XP003001923 *

Also Published As

Publication number Publication date
WO2006017741A2 (en) 2006-02-16

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