WO2005116228A8 - Fermentative herstellung von feinchemikalien - Google Patents

Fermentative herstellung von feinchemikalien

Info

Publication number
WO2005116228A8
WO2005116228A8 PCT/EP2005/005728 EP2005005728W WO2005116228A8 WO 2005116228 A8 WO2005116228 A8 WO 2005116228A8 EP 2005005728 W EP2005005728 W EP 2005005728W WO 2005116228 A8 WO2005116228 A8 WO 2005116228A8
Authority
WO
WIPO (PCT)
Prior art keywords
sugar
liquid medium
metabolic product
containing liquid
starch source
Prior art date
Application number
PCT/EP2005/005728
Other languages
English (en)
French (fr)
Other versions
WO2005116228A2 (de
WO2005116228A3 (de
Inventor
Markus Pompejus
Stephan Freyer
Markus Lohscheidt
Oskar Zelder
Matthias Boy
Original Assignee
Basf Ag
Markus Pompejus
Stephan Freyer
Markus Lohscheidt
Oskar Zelder
Matthias Boy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy filed Critical Basf Ag
Priority to CN2005800224514A priority Critical patent/CN1981045B/zh
Priority to EP05754405A priority patent/EP1753868A2/de
Priority to UAA200614015A priority patent/UA89785C2/uk
Priority to KR1020067024942A priority patent/KR101317064B1/ko
Priority to US11/597,782 priority patent/US9109244B2/en
Priority to AU2005248061A priority patent/AU2005248061B2/en
Priority to BRPI0511601-5A priority patent/BRPI0511601A/pt
Priority to KR1020127020012A priority patent/KR101479583B1/ko
Priority to MXPA06013512 priority patent/MX291201B/es
Priority to EA200602068A priority patent/EA015492B9/ru
Priority to JP2007513814A priority patent/JP4659825B2/ja
Priority to CA002566475A priority patent/CA2566475A1/en
Publication of WO2005116228A2 publication Critical patent/WO2005116228A2/de
Publication of WO2005116228A3 publication Critical patent/WO2005116228A3/de
Publication of WO2005116228A8 publication Critical patent/WO2005116228A8/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/24—Proline; Hydroxyproline; Histidine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Herstellung wenigstens eines mikrobiellen Stoffwechselprodukts mit mindestens 3 C-Atomen oder mit mindestens 2 C-Atomen und mindestens 1 N-Atom durch zuckerbasierte mikrobielle Fermentation, umfassend: a) die Herstellung eines zuckerhaltigen Flüssigmediums mit einem Monosaccharidgehalt von mehr als 20 Gew.-% aus einer Stärkequelle, wobei das zuckerhaltige Flüssigmedium auch nicht-stärkehaltige feste Bestandteile der Stärkequelle umfasst; b) die Fermentation des zuckerhaltigen Flüssigmediums zur Produktion des(der) Stoffwechselprodukte(s); und c) Abreicherung oder Isolierung wenigstens eines Stoffwechselprodukts aus der Fermentationsbrühe, wobei man einen das(die) gewünschte(n) Stoffwechselprodukt(e) produzierenden Mikroorganismenstamm mit dem zuckerhaltigen Flüssigmedium kultiviert, welches man erhält durch: a1) Vermahlen der Stärkequelle; und a2) Verflüssigen des Mahlguts in einer wässrigen Flüssigkeit in Gegenwart mindestens eines Stärke verflüssigenden Enzyms und anschließendes Verzuckern unter Verwendung mindestens eines verzuckernden Enzyms, wobei man mindestens eine Teilmenge des Mahlguts durch kontinuierliche oder diskontinuierliche Zugabe zur wässrigen Flüssigkeit verflüssigt.
PCT/EP2005/005728 2004-05-28 2005-05-27 Fermentative herstellung von feinchemikalien WO2005116228A2 (de)

Priority Applications (12)

Application Number Priority Date Filing Date Title
CN2005800224514A CN1981045B (zh) 2004-05-28 2005-05-27 精细化学品的发酵产生
EP05754405A EP1753868A2 (de) 2004-05-28 2005-05-27 Fermentative herstellung von feinchemikalien unter verwendung eines fermentationsmediums, welches verflüssigte und verzuckerte stärke aus einer vermahlenen stärkequelle als zuckerquelle beinhaltet
UAA200614015A UA89785C2 (uk) 2004-05-28 2005-05-27 Спосіб одержання щонайменше одного продукту мікробіологічного обміну речовин та білкова композиція, яку одержують згідно зі способом
KR1020067024942A KR101317064B1 (ko) 2004-05-28 2005-05-27 정밀 화학물질의 발효 제조
US11/597,782 US9109244B2 (en) 2004-05-28 2005-05-27 Fermentative production of fine chemicals
AU2005248061A AU2005248061B2 (en) 2004-05-28 2005-05-27 Fermentative production of fine chemicals
BRPI0511601-5A BRPI0511601A (pt) 2004-05-28 2005-05-27 processo para a produção de pelo menos um metabólito microbiano, composição de proteìna, e, uso de um meio liquido contendo açúcar
KR1020127020012A KR101479583B1 (ko) 2004-05-28 2005-05-27 정밀 화학물질의 발효 제조
MXPA06013512 MX291201B (es) 2004-05-28 2005-05-27 Produccion fermentativa de compuestos quimicos finos.
EA200602068A EA015492B9 (ru) 2004-05-28 2005-05-27 Способ получения по меньшей мере одного продукта микробного метаболизма, содежащего по крайней мере три атома углерода или по крайней мере два атома углерода и по крайней мере один атом азота, путем микробиологической ферментации сахаров и композиция на основе протеинов, полученная данным способом
JP2007513814A JP4659825B2 (ja) 2004-05-28 2005-05-27 ファインケミカルの発酵生産
CA002566475A CA2566475A1 (en) 2004-05-28 2005-05-27 Fermentative production of fine chemicals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004026152.0 2004-05-28
DE102004026152A DE102004026152A1 (de) 2004-05-28 2004-05-28 Fermentative Herstellung von Feinchemikalien

Publications (3)

Publication Number Publication Date
WO2005116228A2 WO2005116228A2 (de) 2005-12-08
WO2005116228A3 WO2005116228A3 (de) 2006-05-11
WO2005116228A8 true WO2005116228A8 (de) 2007-03-15

Family

ID=35404441

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/005728 WO2005116228A2 (de) 2004-05-28 2005-05-27 Fermentative herstellung von feinchemikalien

Country Status (18)

Country Link
US (1) US9109244B2 (de)
EP (1) EP1753868A2 (de)
JP (1) JP4659825B2 (de)
KR (2) KR101317064B1 (de)
CN (1) CN1981045B (de)
AR (1) AR049122A1 (de)
AU (1) AU2005248061B2 (de)
BR (1) BRPI0511601A (de)
CA (1) CA2566475A1 (de)
DE (1) DE102004026152A1 (de)
EA (1) EA015492B9 (de)
MX (1) MX291201B (de)
MY (1) MY148904A (de)
PH (1) PH12006502292B1 (de)
TW (1) TWI386488B (de)
UA (1) UA89785C2 (de)
WO (1) WO2005116228A2 (de)
ZA (1) ZA200610738B (de)

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EA015492B1 (ru) 2011-08-30
TWI386488B (zh) 2013-02-21
EP1753868A2 (de) 2007-02-21
AU2005248061B2 (en) 2010-02-11
PH12006502292B1 (en) 2014-02-13
US20090162892A1 (en) 2009-06-25
CA2566475A1 (en) 2005-12-08
DE102004026152A1 (de) 2005-12-15
AU2005248061A1 (en) 2005-12-08
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AR049122A1 (es) 2006-06-28
MX291201B (es) 2011-10-20
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UA89785C2 (uk) 2010-03-10
CN1981045B (zh) 2012-09-26
KR101317064B1 (ko) 2013-10-16
EA200602068A1 (ru) 2007-06-29
US9109244B2 (en) 2015-08-18
WO2005116228A2 (de) 2005-12-08
WO2005116228A3 (de) 2006-05-11
JP4659825B2 (ja) 2011-03-30
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