WO2005116074A3 - Nucleoproteins displaying native t cell receptor libraries - Google Patents

Nucleoproteins displaying native t cell receptor libraries Download PDF

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Publication number
WO2005116074A3
WO2005116074A3 PCT/GB2005/001948 GB2005001948W WO2005116074A3 WO 2005116074 A3 WO2005116074 A3 WO 2005116074A3 GB 2005001948 W GB2005001948 W GB 2005001948W WO 2005116074 A3 WO2005116074 A3 WO 2005116074A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleoproteins
cell receptor
variable domain
native
native tcr
Prior art date
Application number
PCT/GB2005/001948
Other languages
French (fr)
Other versions
WO2005116074A2 (en
Inventor
Jonathan Michael Boulter
Torben Bent Andersen
Bent Karsten Jakobsen
Peter Eamon Molloy
Yi Li
Original Assignee
Avidex Ltd
Jonathan Michael Boulter
Torben Bent Andersen
Bent Karsten Jakobsen
Peter Eamon Molloy
Yi Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avidex Ltd, Jonathan Michael Boulter, Torben Bent Andersen, Bent Karsten Jakobsen, Peter Eamon Molloy, Yi Li filed Critical Avidex Ltd
Priority to EP05745014A priority Critical patent/EP1781702A2/en
Publication of WO2005116074A2 publication Critical patent/WO2005116074A2/en
Publication of WO2005116074A3 publication Critical patent/WO2005116074A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/7051—T-cell receptor (TcR)-CD3 complex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70539—MHC-molecules, e.g. HLA-molecules

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A diverse library of nucleoproteins each displaying on its surface a polypeptide comprising a native TCR α variable domain sequence or native TCR ß variable domain sequence, in which library the diversity resides in the variety of native TCR α variable domain or native TCR ß variable domain sequences displayed. Such a library is useful for identifying a ligand or ligands of a target peptide-MHC or CD1-antigen.
PCT/GB2005/001948 2004-05-26 2005-05-18 Nucleoproteins displaying native t cell receptor libraries WO2005116074A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05745014A EP1781702A2 (en) 2004-05-26 2005-05-18 Nucleoproteins displaying native t cell receptor libraries

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0411771.9 2004-05-26
GB0411771A GB0411771D0 (en) 2004-05-26 2004-05-26 Nucleoproteins displaying native T cell receptor libraries

Publications (2)

Publication Number Publication Date
WO2005116074A2 WO2005116074A2 (en) 2005-12-08
WO2005116074A3 true WO2005116074A3 (en) 2006-01-19

Family

ID=32671107

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/001948 WO2005116074A2 (en) 2004-05-26 2005-05-18 Nucleoproteins displaying native t cell receptor libraries

Country Status (3)

Country Link
EP (1) EP1781702A2 (en)
GB (1) GB0411771D0 (en)
WO (1) WO2005116074A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7471867B2 (en) 2014-03-14 2024-04-22 イムノコア リミテッド TCR Library

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2338509B1 (en) 2003-06-27 2014-06-18 International Institute of Cancer Immunology, Inc. Method of selecting patients suitable for WT1 vaccine
EP2857508A3 (en) 2007-03-05 2015-04-15 International Institute of Cancer Immunology, Inc. Cancer antigen-specific T-cell receptor gene, peptide encoded by the gene, and use of them
EP2746393A4 (en) 2011-06-28 2015-06-10 Int Inst Cancer Immunology Inc Receptor gene for peptide cancer antigen-specific t cell
EA201792661A1 (en) 2015-06-01 2018-05-31 Медиджин Иммьюнотерапиз Гмбх METHOD OF OBTAINING ANTIBODIES AGAINST T-CELL RECEPTOR
WO2016193299A1 (en) * 2015-06-01 2016-12-08 Medigene Immunotherapies Gmbh T cell receptor library
GB201516277D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR libraries
GB201516276D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516272D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516269D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516270D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516265D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516275D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516274D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
WO2017089786A1 (en) 2015-11-23 2017-06-01 Immunocore Limited Peptides
JP6697562B2 (en) 2015-12-23 2020-05-20 メディジーン イミュノテラピーズ ゲーエムベーハー Dendritic cell composition

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998039482A1 (en) * 1997-03-07 1998-09-11 Sunol Molecular Corporation Fusion proteins comprising bacteriophage coat protein and a single-chain t cell receptor
WO1998048008A1 (en) * 1997-04-23 1998-10-29 Plueckthun Andreas Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
WO1999018129A1 (en) * 1997-10-02 1999-04-15 Sunol Molecular Corporation Soluble single-chain t-cell receptor proteins
WO2001062908A2 (en) * 2000-02-22 2001-08-30 Ahuva Nissim Chimeric and tcr phage display libraries, chimeric and tcr reagents and methods of use thereof
WO2001092291A2 (en) * 2000-05-30 2001-12-06 Galim - Galil Immunology Ltd. Antibody and t-cell receptor libraries
WO2003020763A2 (en) * 2001-08-31 2003-03-13 Avidex Limited Soluble t cell receptor
WO2004044004A2 (en) * 2002-11-09 2004-05-27 Avidex Limited T cell receptor display

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0411773D0 (en) * 2004-05-26 2004-06-30 Avidex Ltd Method for the identification of polypeptides which bind to a given peptide mhc complex or cd 1-antigen complex

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998039482A1 (en) * 1997-03-07 1998-09-11 Sunol Molecular Corporation Fusion proteins comprising bacteriophage coat protein and a single-chain t cell receptor
WO1998048008A1 (en) * 1997-04-23 1998-10-29 Plueckthun Andreas Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
WO1999018129A1 (en) * 1997-10-02 1999-04-15 Sunol Molecular Corporation Soluble single-chain t-cell receptor proteins
WO2001062908A2 (en) * 2000-02-22 2001-08-30 Ahuva Nissim Chimeric and tcr phage display libraries, chimeric and tcr reagents and methods of use thereof
WO2001092291A2 (en) * 2000-05-30 2001-12-06 Galim - Galil Immunology Ltd. Antibody and t-cell receptor libraries
WO2003020763A2 (en) * 2001-08-31 2003-03-13 Avidex Limited Soluble t cell receptor
WO2004044004A2 (en) * 2002-11-09 2004-05-27 Avidex Limited T cell receptor display

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AMSTUTZ PATRICK ET AL: "In vitro display technologies: Novel developments and applications", CURRENT OPINION IN BIOTECHNOLOGY, vol. 12, no. 4, August 2001 (2001-08-01), pages 400 - 405, XP002351987, ISSN: 0958-1669 *
BOULTER JONATHAN M ET AL: "Stable, soluble T-cell receptor molecules for crystallization and therapeutics", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 16, no. 9, September 2003 (2003-09-01), pages 707 - 711, XP002275339, ISSN: 0269-2139 *
KIEKE M C ET AL: "High affinity T cell receptors from yeast display libraries block T cell activation by superantigens", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 307, no. 5, 13 April 2001 (2001-04-13), pages 1305 - 1315, XP004466060, ISSN: 0022-2836 *
ONDA T ET AL: "A PHAGE DISPLAY SYSTEM FOR DETECTION OF T CELL RECEPTOR-ANTIGEN INTERACTIONS", MOLECULAR IMMUNOLOGY, ELMSFORD, NY, US, vol. 32, no. 17/18, 1995, pages 1387 - 1397, XP002912582, ISSN: 0161-5890 *
See also references of EP1781702A2 *
WEIDANZ J A ET AL: "Display of functional alphabeta single-chain T-cell receptor molecules on the surface of bacteriophage", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 221, no. 1-2, December 1998 (1998-12-01), pages 59 - 76, XP004153560, ISSN: 0022-1759 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7471867B2 (en) 2014-03-14 2024-04-22 イムノコア リミテッド TCR Library

Also Published As

Publication number Publication date
EP1781702A2 (en) 2007-05-09
WO2005116074A2 (en) 2005-12-08
GB0411771D0 (en) 2004-06-30

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