WO2005103299A3 - Rnai-based target identification and validation - Google Patents

Rnai-based target identification and validation Download PDF

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Publication number
WO2005103299A3
WO2005103299A3 PCT/US2005/013546 US2005013546W WO2005103299A3 WO 2005103299 A3 WO2005103299 A3 WO 2005103299A3 US 2005013546 W US2005013546 W US 2005013546W WO 2005103299 A3 WO2005103299 A3 WO 2005103299A3
Authority
WO
WIPO (PCT)
Prior art keywords
rnai
validation
target identification
based target
identification
Prior art date
Application number
PCT/US2005/013546
Other languages
French (fr)
Other versions
WO2005103299A2 (en
Inventor
Peter Blume-Jensen
Original Assignee
Applied Research Systems
Peter Blume-Jensen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems, Peter Blume-Jensen filed Critical Applied Research Systems
Priority to AU2005236064A priority Critical patent/AU2005236064A1/en
Priority to CA002562674A priority patent/CA2562674A1/en
Priority to JP2007509612A priority patent/JP2007533326A/en
Priority to EP05737635A priority patent/EP1756304A2/en
Publication of WO2005103299A2 publication Critical patent/WO2005103299A2/en
Publication of WO2005103299A3 publication Critical patent/WO2005103299A3/en
Priority to IL178736A priority patent/IL178736A0/en
Priority to NO20065312A priority patent/NO20065312L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides methods of using RNAi in a high throughput screening for the identification and validation of drug targets in a plysiological, disease-relevant context.
PCT/US2005/013546 2004-04-20 2005-04-20 Rnai-based target identification and validation WO2005103299A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2005236064A AU2005236064A1 (en) 2004-04-20 2005-04-20 RNAI-based target identification and validation
CA002562674A CA2562674A1 (en) 2004-04-20 2005-04-20 Rnai-based target identification and validation
JP2007509612A JP2007533326A (en) 2004-04-20 2005-04-20 Identification and validation of RNAi-based targets
EP05737635A EP1756304A2 (en) 2004-04-20 2005-04-20 Rnai-based target identification and validation
IL178736A IL178736A0 (en) 2004-04-20 2006-10-19 RNAi-BASED TARGET IDENTIFICATION AND VALIDATION
NO20065312A NO20065312L (en) 2004-04-20 2006-11-20 RNAi-based template identification and validation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56362804P 2004-04-20 2004-04-20
US60/563,628 2004-04-20

Publications (2)

Publication Number Publication Date
WO2005103299A2 WO2005103299A2 (en) 2005-11-03
WO2005103299A3 true WO2005103299A3 (en) 2006-02-16

Family

ID=35197560

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/013546 WO2005103299A2 (en) 2004-04-20 2005-04-20 Rnai-based target identification and validation

Country Status (7)

Country Link
EP (1) EP1756304A2 (en)
JP (1) JP2007533326A (en)
AU (1) AU2005236064A1 (en)
CA (1) CA2562674A1 (en)
IL (1) IL178736A0 (en)
NO (1) NO20065312L (en)
WO (1) WO2005103299A2 (en)

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
LAVERY K S ET AL: "ANTISENSE AND RNAI: POWERFUL TOOLS IN DRUG TARGET DISCOVERY AND VALIDATION", CURRENT OPINION IN DRUG DISCOVERY AND DEVELOPMENT, CURRENT DRUGS, LONDON, GB, vol. 6, no. 4, July 2003 (2003-07-01), pages 561 - 569, XP008054844, ISSN: 1367-6733 *
LUM LAWRENCE ET AL: "Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.", SCIENCE (WASHINGTON D C), vol. 299, no. 5615, 28 March 2003 (2003-03-28), pages 2039 - 2045, XP002353311, ISSN: 0036-8075 *
ROZEMA D B ET AL: "siRNA delivery technologies for mammalian systems", TARGETS, ELSEVIER, vol. 2, no. 6, 1 December 2003 (2003-12-01), pages 253 - 260, XP004886693, ISSN: 1477-3627 *
TUSCHL T: "Expanding small RNA interference", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 20, no. 5, May 2002 (2002-05-01), pages 446 - 448, XP002232258, ISSN: 1087-0156 *
WANG SHENLIANG ET AL: "Tools for target identification and validation", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 8, no. 4, 17 June 2004 (2004-06-17) - 17 June 2004 (2004-06-17), pages 371 - 377, XP002353312, ISSN: 1367-5931 *
WILLINGHAM AARRON T ET AL: "RNAi and HTS: exploring cancer by systematic loss-of-function", ONCOGENE, vol. 23, no. 51, 1 November 2004 (2004-11-01), pages 8392 - 8400, XP002353313, ISSN: 0950-9232 *
XIA H ET AL: "siRNA-mediated gene silencing in vitro and in vivo", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 20, no. 10, October 2002 (2002-10-01), pages 1006 - 1010, XP002251054, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2005103299A2 (en) 2005-11-03
JP2007533326A (en) 2007-11-22
IL178736A0 (en) 2007-02-11
EP1756304A2 (en) 2007-02-28
NO20065312L (en) 2006-11-20
CA2562674A1 (en) 2005-11-03
AU2005236064A1 (en) 2005-11-03

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