WO2005085417A3 - Bakterielles system zum proteintransport in eukaryontische zellen - Google Patents

Bakterielles system zum proteintransport in eukaryontische zellen Download PDF

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Publication number
WO2005085417A3
WO2005085417A3 PCT/DE2005/000330 DE2005000330W WO2005085417A3 WO 2005085417 A3 WO2005085417 A3 WO 2005085417A3 DE 2005000330 W DE2005000330 W DE 2005000330W WO 2005085417 A3 WO2005085417 A3 WO 2005085417A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
eukaryotic cells
bacterial
transport
hpab
Prior art date
Application number
PCT/DE2005/000330
Other languages
English (en)
French (fr)
Other versions
WO2005085417A2 (de
Inventor
Ulla Bonas
Daniela Buettner
Original Assignee
Univ Halle Wittenberg
Ulla Bonas
Daniela Buettner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Halle Wittenberg, Ulla Bonas, Daniela Buettner filed Critical Univ Halle Wittenberg
Priority to DE112005001016T priority Critical patent/DE112005001016A5/de
Publication of WO2005085417A2 publication Critical patent/WO2005085417A2/de
Publication of WO2005085417A3 publication Critical patent/WO2005085417A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Der Erfindung liegt das Problem zu Grunde, ein System zum gezielten Transport von Proteinen in eukaryontische Zellen zu entwickeln. Der spezifische Transport von Proteinen in eukaryontische Zellen erfolgt durch den Einsatz eines Typ III-Sekretionssystems unter Verwendung von Bakterienstämmen, die in hpaB oder homologen Genen mutiert sind. Ausgehend von einem Gramnegativen pathogenen Bakterienstamm wird ein nicht-pathogener Stamm generiert, der im hpaB-Gen bzw. einem homologen Gen mutiert ist, wobei dieser keine signifikante Translokation von bakteriellen Effektorproteinen vermittelt. Überexprimierte Effektorproteine oder Proteine, die das Typ 111-Sekretionssignal eines Nicht-Effektorproteins besitzen, können jedoch unter Verwendung des bakterieneigenen TTSS in eukaryontische Zellen transloziert werden. Das erfindungsgemässe bakterielle System wird zum Transport von bakteriellen Proteinen in eukaryontische Zellen eingesetzt, um zelluläre Prozesse wie Genexpression, Wachstum, Entwicklung und Abwehr-Resistenzmechanismen zu beeinflussen bzw. zu modifizieren.
PCT/DE2005/000330 2004-03-02 2005-02-26 Bakterielles system zum proteintransport in eukaryontische zellen WO2005085417A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112005001016T DE112005001016A5 (de) 2004-03-02 2005-02-26 Bakterielles System zum Proteintransport in eukaryontische Zellen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200410010023 DE102004010023A1 (de) 2004-03-02 2004-03-02 Bakterielles System zum Proteintransport in eukaryontische Zellen
DE102004010023.3 2004-03-02

Publications (2)

Publication Number Publication Date
WO2005085417A2 WO2005085417A2 (de) 2005-09-15
WO2005085417A3 true WO2005085417A3 (de) 2006-01-12

Family

ID=34877228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2005/000330 WO2005085417A2 (de) 2004-03-02 2005-02-26 Bakterielles system zum proteintransport in eukaryontische zellen

Country Status (2)

Country Link
DE (2) DE102004010023A1 (de)
WO (1) WO2005085417A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2504430A4 (de) 2009-11-27 2013-06-05 Basf Plant Science Co Gmbh Chimäre endonukleasen und ihre verwendung
CA2781693C (en) 2009-11-27 2018-12-18 Basf Plant Science Company Gmbh Chimeric endonucleases and uses thereof
EP2612918A1 (de) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta-Rekombination
EP4200448A1 (de) * 2020-08-20 2023-06-28 Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement Modifizierte xanthomonas und verfahren zur beurteilung der krankheitserregererkennung durch pflanzen

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000002996A2 (en) * 1998-07-10 2000-01-20 Cornell Research Foundation, Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000002996A2 (en) * 1998-07-10 2000-01-20 Cornell Research Foundation, Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BÜTTNER DANIELA ET AL: "Getting across--bacterial type III effector proteins on their way to the plant cell.", THE EMBO JOURNAL. 15 OCT 2002, vol. 21, no. 20, 15 October 2002 (2002-10-15), pages 5313 - 5322, XP002345956, ISSN: 0261-4189 *
BÜTTNER DANIELA ET AL: "HpaB from Xanthomonas campestris pv. vesicatoria acts as an exit control protein in type III-dependent protein secretion.", MOLECULAR MICROBIOLOGY. NOV 2004, vol. 54, no. 3, November 2004 (2004-11-01), pages 755 - 768, XP002345957, ISSN: 0950-382X *
KIM JUNG-GUN ET AL: "Characterization of the Xanthomonas axonopodis pv. glycines Hrp pathogenicity island.", JOURNAL OF BACTERIOLOGY, vol. 185, no. 10, May 2003 (2003-05-01), pages 3155 - 3166, XP002345955, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
DE112005001016A5 (de) 2007-05-24
WO2005085417A2 (de) 2005-09-15
DE102004010023A1 (de) 2005-09-22

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