WO2005085279A1 - Gene et proteine osasr1 inductibles par le stress et renforçant la tolerance au stress abiotique - Google Patents

Gene et proteine osasr1 inductibles par le stress et renforçant la tolerance au stress abiotique Download PDF

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Publication number
WO2005085279A1
WO2005085279A1 PCT/KR2005/000663 KR2005000663W WO2005085279A1 WO 2005085279 A1 WO2005085279 A1 WO 2005085279A1 KR 2005000663 W KR2005000663 W KR 2005000663W WO 2005085279 A1 WO2005085279 A1 WO 2005085279A1
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WIPO (PCT)
Prior art keywords
stress
osasrl
plant
cold
plants
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PCT/KR2005/000663
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English (en)
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Seong Ryong Kim
Soo Jin Kim
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Industry-University Cooperation Foundation Sogang University
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Publication of WO2005085279A1 publication Critical patent/WO2005085279A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B23—MACHINE TOOLS; METAL-WORKING NOT OTHERWISE PROVIDED FOR
    • B23B—TURNING; BORING
    • B23B31/00—Chucks; Expansion mandrels; Adaptations thereof for remote control
    • B23B31/02—Chucks
    • B23B31/24—Chucks characterised by features relating primarily to remote control of the gripping means
    • B23B31/28—Chucks characterised by features relating primarily to remote control of the gripping means using electric or magnetic means in the chuck
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
    • G05B19/00—Programme-control systems
    • G05B19/02—Programme-control systems electric
    • G05B19/18—Numerical control [NC], i.e. automatically operating machines, in particular machine tools, e.g. in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form
    • G05B19/406—Numerical control [NC], i.e. automatically operating machines, in particular machine tools, e.g. in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form characterised by monitoring or safety

Definitions

  • CBLl CalcineurinB-like 1 ⁇ CBLl gene is highly inducible by drought, salt and cold stresses.
  • CBLl-overexpressing plants showed enhanced tolerance to salt and drought (Cheong, Y.H., Kim, K.N. , Pandey, G.K., Gupta, R., Grant, J.J., andLuan, S.2003.
  • CBLl a calciumsensor that differentially regulates salt, drought, and cold responses in Arabidopsis . Plant Cell 15:1833-1845) .
  • the Asr genes which are responsive to ABA, osmotic stress, and ripening, have been identified in various species, including tomato, potato, apricot, loblolly pine, lily, maize, pummelo, grape and rice (Iusem et al . , 1993; Canel et al . , 1995; Silhavy et al . , 1995; Wang et al . , 1998; Chang et al . , 1996; Mbeguie-A-Mbeguie et al . , 1997;
  • ASR Late Embryo Abundant
  • dehydrin proteins suggests a possible role of ASR in the seed development (Maskin et al . , 2001; Silhavy et al . , 1995) .
  • Many known ASR proteins contain two conserved regions of aputative Zn-binding site at the N-terminal region andaputative nuclear localization sequence (NLS) at the C-terminal region of ⁇ 70 amino acids (Cakir et al . , 2003; Silhavy et al . , 1995) .
  • the vector system of this invention may be constructed according to the known methods in the art as described in Sambrook et al . , Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press (2001) , which is incorporated herein by reference.
  • the vector may be constructed for cloning or expression.
  • the vector may be constructed for use in prokaryotic or eukaryotic host cells.
  • the vector is constructed for expression in prokaryotic cells, it generally carries a strong promoter to initiate transcription (e.g., pL ⁇ promoter, trp promoter, lac promoter, T7 promoter and tac promoter) , a riboso e binding site for translation initiation and a transcription/translation termination sequence.
  • a promoter and operator i in operon for tryptophan biosynthesis in E. coli (Yanofsky, C, J.
  • An embodiment of a method using Agrobacterium tumefaciens-binary vector system comprises the steps of : (a') inoculatinganexplantmaterial from the plant with Agrobacterium tumefaciens harboring a vector, in which the vector is capable of being inserted into a genome of a cell from the plant and contains the following nucleotide sequences: (i) the nucleotide sequence encoding abiotic stress-inducible OsAsrl protein; (ii) a promoter that functions in plant cells to cause the production of an RNA molecule operably linked to the nucleotide sequence of (i) ; and (iii) a 3 ' -non-translated region that functions in plant cells to cause the polyadenylation of the 3 '-end of the RNA molecule;
  • Figure 4d represents analysis of OsAsrl expression under various abiotic stresses and ABA. SalT was used for the drought-, cold-, salt-, and ABA-response controls . Transcript of rice actin gene shows an 'internal control for PCR analysis.
  • Figure 4e represents analysis of OsAsrl expression levels using real-time PCR. Error bars represent standard deviation. 8-d seedlings were treated with 4°C (c) , 250 mM NaCl (s) , drought (d) or 100 ⁇ M ABA (A) and then were periodically harvested.
  • Figures 5a and 5b represent in si tu localization of OsAsrl mRNA.
  • Cross sections of the rice flowers of 4 days before heading and leaves exposed to 12 °C for 4 days were hybridized with digoxygenin-labeled antisense (A, C, E, and G) or sense (B, D, F, and H) OsAsrl probes. Higher magnification of the cross section is shown (C, D, G, and H) .
  • An, anther; lm, lemma; pa, palea; l.e.p, lower epidermis of palea; LVB, large vascular bundle; mc, motor cell; xy, xylem; ph, phloem; me, mesophyll . Bar 0.3 mm.
  • Figures 6a-6c represent analysis of transgenic plants expressing OsAsrl in sense and antisense oritentation.
  • Figure 6a represents construction of OsAsrl sense (pSK167) and antisense (pSK168) expression vector for rice transformation.
  • P UBI maize ubiqutin promoter; P 3Ss / CaMV
  • RNA in situ hybridization Rice flowers and leaves were fixed overnight at 4 °C in 2% (wt/vol) paraformaldehyde plus 2.5% (vol/vol) glutaraldehyde in a 50-mM PIPES buffer (pH 7.2). The fixed tissues were dehydrated by graded concentrations of ethanol, then embedded in a paraplast medium (Oxford labware, USA) . The embedded tissues were sliced into 7- ⁇ m sections with a rotary microtome (Leica, Germany) , and each section was attached to a silanized glass slide (Matsunami, Japan) . Paraffin was removed through a graded series of ethanol concentrations, and the samples were dried for 1 h.
  • the transcript was present at high levels in the shoots and roots of seedlings, sheath of flag leaves, and most abundantly in the internodes between node I and II, demonstrating organ-preferential expression.
  • OsAsrl was expressed at the basal level in the leaves of mature plants, but more abundantly in mature flowers.
  • low temperatures elevated overall transcript levels in both organ types ( Figure 4b) .
  • Cold treatment increased the transcript level also at the seedling stage, but more significantly at 12 °C instead of 4 °C ( Figure 4c) .
  • the cold-responsive OsAsrl accumulation was restricted to shoots, demonstrating the organ-specific stress response of the gene expression. Because OsAsrl is ABA-inducible (Vaidyanathan et al .
  • Chlorophyll fluorescence of transgenic plants under cold stress Chlorophyll fluorescence was measured as an indicator of chilling tolerance after cold treatment (4°C) .
  • the ratio of Fv to Fm which represents the activity of Photosystem II, is used to assess functional damage in plants (Genty et al . , 1989) .
  • Fv/Fm progressively decreased following chilling. This decline illustrates the extent of photoinhibition causedby cold stress (Krause, 1994) . Values for Fv/Fm had been 0.84+0.01 before stress was induced. Following the 6-h cold treatment, Fv/Fm decreased slightly, and no significant difference in values was found between the transgenic and the wild-type segregants.
  • Arabidopsis thaliana CBFl encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad. Sci. USA 94: 1035-1040.

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Manufacturing & Machinery (AREA)
  • Human Computer Interaction (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Automation & Control Theory (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mechanical Engineering (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention porte sur le gène et la protéine OsAsr1 inductibles par le stress abiotique et renforçant la tolérance de plantes au froid, au sel et à la sécheresse là où l'OsAsr1 est surexprimé dans une plante. Le gène et la protéine OsAsr1 de l'invention s'avèrent donc très utiles pour renforcer la tolérance de plantes au froid, au sel et à la sécheresse.
PCT/KR2005/000663 2004-03-09 2005-03-09 Gene et proteine osasr1 inductibles par le stress et renforçant la tolerance au stress abiotique WO2005085279A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2004-0015804 2004-03-09
KR1020040015804A KR20050117630A (ko) 2004-03-09 2004-03-09 저온 내성을 증진시키는 저온-유도성 ＯｓＡｓｒ1 유전자및 단백질

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WO2005085279A1 true WO2005085279A1 (fr) 2005-09-15

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114292318A (zh) * 2021-12-31 2022-04-08 江西农业大学 一种增强植物非生物胁迫抗性的蛋白、编码基因、引物对、表达载体及其应用
CN116891521A (zh) * 2023-09-11 2023-10-17 中国科学院昆明植物研究所 调控植物抗旱性和耐盐性的SpDREB2B蛋白及其应用

Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
WO2014025137A1 (fr) * 2012-08-06 2014-02-13 명지대학교 산학협력단 Gène uip1 pour augmenter la résistance de plantes au stress de sécheresse et utilisation de celui-ci

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BR9610780A (pt) 1995-10-12 1999-07-13 Cornnel Research Foundation In Process de produzir uma celula ou protoplasto de planta cereal planta cereal transgenica semente processo de aumentar a tolerancia de uma planta cereal e celula ou protoplasto de planta cereal
JP4582853B2 (ja) 2000-02-25 2010-11-17 岩手県 グルタチオン−ｓ−トランスフェラーゼ遺伝子を導入した低温抵抗性イネ
KR100614418B1 (ko) * 2004-02-04 2006-08-21 대한민국 환경스트레스 칼슘신호전달 관련 인산화 효소와 유전자

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AJAY K.G. ET AL: "Trealose accumulation in rice plants confers high tolerance levels to different abiotic stresses.", PNAS., vol. 99, November 2002 (2002-11-01), pages 15898 - 15903 *
DATABASE GENPEPT [online] 17 March 2000 (2000-03-17), Database accession no. (AAB96681) *
HONG J.K. ET AL: "Induction by pathogen, salt and drought for a basic class II chitinase mRNA and its in situ localization in peper (Capsicum annuum).", PHYSIOL. PLANT., vol. 114, no. 4, April 2002 (2002-04-01), pages 549 - 558 *
KIM T.E. ET AL: "ABA and polymines act independently in primary leavels of cold-stressed tomato (Lycopersicon esculentum).", PHYSIOL.PLANT., vol. 115, no. 3, July 2002 (2002-07-01), pages 370 - 376 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114292318A (zh) * 2021-12-31 2022-04-08 江西农业大学 一种增强植物非生物胁迫抗性的蛋白、编码基因、引物对、表达载体及其应用
CN114292318B (zh) * 2021-12-31 2023-05-05 江西农业大学 一种增强植物非生物胁迫抗性的蛋白、编码基因、引物对、表达载体及其应用
CN116891521A (zh) * 2023-09-11 2023-10-17 中国科学院昆明植物研究所 调控植物抗旱性和耐盐性的SpDREB2B蛋白及其应用
CN116891521B (zh) * 2023-09-11 2023-11-28 中国科学院昆明植物研究所 调控植物抗旱性和耐盐性的SpDREB2B蛋白及其应用

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KR100695072B1 (ko) 2007-03-14
KR20060043798A (ko) 2006-05-15
KR20050117630A (ko) 2005-12-15

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