WO2005059505A3 - Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real - Google Patents

Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real Download PDF

Info

Publication number
WO2005059505A3
WO2005059505A3 PCT/ES2004/070105 ES2004070105W WO2005059505A3 WO 2005059505 A3 WO2005059505 A3 WO 2005059505A3 ES 2004070105 W ES2004070105 W ES 2004070105W WO 2005059505 A3 WO2005059505 A3 WO 2005059505A3
Authority
WO
WIPO (PCT)
Prior art keywords
kit
allele
hla
real time
genotypification
Prior art date
Application number
PCT/ES2004/070105
Other languages
English (en)
French (fr)
Other versions
WO2005059505A2 (es
Inventor
Otero Manuel Juan
Ponces Natalia Casamitjana
Borrell Ricardo Pujol
Oriol Roger Colobran
Rivera Eduardo Palou
Canet Maria Rosa Faner
Crusafont Ana Ribera
Original Assignee
Univ Barcelona Autonoma
Otero Manuel Juan
Ponces Natalia Casamitjana
Borrell Ricardo Pujol
Oriol Roger Colobran
Rivera Eduardo Palou
Canet Maria Rosa Faner
Crusafont Ana Ribera
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Barcelona Autonoma, Otero Manuel Juan, Ponces Natalia Casamitjana, Borrell Ricardo Pujol, Oriol Roger Colobran, Rivera Eduardo Palou, Canet Maria Rosa Faner, Crusafont Ana Ribera filed Critical Univ Barcelona Autonoma
Priority to EP04805142A priority Critical patent/EP1741791A2/en
Publication of WO2005059505A2 publication Critical patent/WO2005059505A2/es
Publication of WO2005059505A3 publication Critical patent/WO2005059505A3/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención se relaciona con un método para determinar alelos del grupo alélico 27 del locus B del antígeno leucocitario humano (HLA-B27) a partir de una muestra de ácido nucleico y con un kit para Ilevar a cabo este método. El kit comprende cebadores específicos de alelo para usar en la reacción en cadena de la polimerasa (PCR) en tiempo real, y sondas específicas de alelo marcadas fluorescentemente, o un agente intercalador, para detectar señales de fluorescencia. El análisis se completa con la determinación de las temperaturas de fusión de las secuencias amplificadas de ácidos nucleicos. Supera algunas de las limitaciones de los métodos convencionales de tipificación basados en ADN, en términos de tiempo, posibilidad de automatización, y aumento de la resolución, mientras se reduce el número de reacciones PCR. Es útil p.ej. para determinar la predisposición a una enfermedad específica.
PCT/ES2004/070105 2003-12-17 2004-12-03 Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real WO2005059505A2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04805142A EP1741791A2 (en) 2003-12-17 2004-12-03 Method and kit for the genotypification of hla-b27 based on real time pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200400009 2003-12-17
ES200400009 2003-12-17

Publications (2)

Publication Number Publication Date
WO2005059505A2 WO2005059505A2 (es) 2005-06-30
WO2005059505A3 true WO2005059505A3 (es) 2005-12-29

Family

ID=34684848

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2004/070105 WO2005059505A2 (es) 2003-12-17 2004-12-03 Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real

Country Status (2)

Country Link
EP (1) EP1741791A2 (es)
WO (1) WO2005059505A2 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2276630B1 (es) * 2005-12-13 2008-06-16 Universitat Autonoma De Barcelona Metodo y kit para genotipificacion de hla-b basados en la pcr en tiempo real.
KR101352470B1 (ko) 2011-05-25 2014-01-16 김현숙 인간 백혈구 항원 ｂ２７과 ｂ５１ 유전자형 진단을 위한 프라이머 및 프로브, 이를 이용한 검사 방법 및 이를 포함하는 검사 키트
CN103255209A (zh) * 2013-03-27 2013-08-21 天津市秀鹏生物技术开发有限公司 用于检测人类白细胞hla-b27抗原基因的引物组及试剂盒
CA2933252A1 (en) * 2013-12-10 2015-06-18 Conexio Genomics Pty Ltd Methods and probes for identifying gene alleles
CN109182491B (zh) * 2018-09-04 2021-10-22 江苏中济万泰生物医药有限公司 一种人类白细胞hla-b*27基因分型引物组及应用
CN110632071A (zh) * 2019-09-18 2019-12-31 南京华银医学检验所有限公司 一种白细胞抗原b27检测方法及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1229128A1 (en) 2001-01-31 2002-08-07 Boehringer Mannheim Gmbh New method for genotype determination

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BERNARD PS ET AL: "Homogeneous amplification and variant detection by fluorescent hybridization probes.", CLIN CHEM., vol. 46, no. 2, 2000, pages 147 - 148, XP008072923 *
BON MA ET AL: "Genotyping of HLA-B27 by real-time PCR without hybridization probes.", CLIN CHEM., vol. 46, no. 7, 2000, pages 1000 - 1002, XP008072915 *
FANER R ET AL: "HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR.", HUM IMMUNOL., vol. 65, no. 8, 2004, pages 826 - 838, XP004546057 *
GARCIA-FERNANDEZ S ET AL: "New insights regarding HLA-B27 diversity in the Asian population.", TISSUE ANTIGENS., vol. 58, no. 4, 2001, pages 259 - 262, XP008072921 *
GELSTHORPE AR ET AL: "High-throughput class I HLA genotyping using fluorescence resonance energy transfer (FRET) probes and sequence-specific primer-polymerase chain reaction (SSP-PCR).", TISSUE ANTIGENS., vol. 54, no. 6, 1999, pages 603 - 614, XP008072919 *
OLERUP O ET AL: "HLA-B27 typing by a group-specific PCR amplification.", TISSUE ANTIGENS., vol. 43, no. 4, 1994, pages 253 - 256, XP008072922 *
PAPP AC ET AL: "Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves.", BIOTECHNIQUES., vol. 34, no. 5, 2003, pages 1068 - 1072, XP001208020 *
TIEMANN C ET AL: "Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology.", CLIN LAB., vol. 47, no. 3-4, 2001, pages 131 - 134, XP008072918 *

Also Published As

Publication number Publication date
EP1741791A2 (en) 2007-01-10
EP1741791A9 (en) 2009-07-29
WO2005059505A2 (es) 2005-06-30

Similar Documents

Publication Publication Date Title
US7078168B2 (en) Method for determining allele frequencies
CA2239896C (en) Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of hla types
EP1241266A1 (en) Method of detecting nucleotide polymorphism
Syvänen et al. A primer-guided nucleotide incorporation assay in the genotyping of apolipoprotein E
US8507205B2 (en) Single cell analysis by polymerase cycling assembly
EP0907752B1 (en) Method for determination of nucleic acid sequences and diagnostic applications thereof
CN107385040B (zh) 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
US20030148301A1 (en) Method of detecting nucleotide polymorphism
ATE328115T1 (de) Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden
Csako Present and future of rapid and/or high-throughput methods for nucleic acid testing
DE69528706T2 (de) Gekoppeltes ampflikation- und ligationverfahren
CA2497740A1 (en) Multiplexed analysis of polymorphic loci by probe elongation-mediated detection
EP1926834A2 (en) A method for testing milk
CA2908619A1 (en) Non-invasive early detection of solid organ transplant rejection by quantitative analysis of mixtures by deep sequencing of hla gene amplicons using next generation systems
CA3108364A1 (en) Method for carrying out real-time pcr
WO2005059505A3 (es) Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real
EP1964929B1 (en) Hla-b genotyping method and kit based on real-time pcr
EP1300473B1 (en) Method of detecting nucleotide polymorphism
US11530455B2 (en) Penta E polymorphisms for human identification
Milla et al. Use of the Restriction Enzyme Ava I and Exo− Bst Polymerase in Strand Displacement Amplification
Bakar et al. Optimisation of polymerase chain reaction conditions to amplify D-loop region in the Malaysian mousedeer genomic DNA
US20030104421A1 (en) Methods and compositions for nucleic acid amplification
US20060078881A1 (en) Method and kit for detection of mutations in mitochondrial dna
Hue-Roye et al. Principles of PCR-based assays
WO2007039101A1 (en) Method for the quantification of nucleic acids, in particular bisulfite treated dna

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 2004805142

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004805142

Country of ref document: EP